64 results about "Rice root" patented technology

The invention belongs to the technical field of crop pollution prevention, and particularly relates to a rice heavy metal adsorption and isolation method based on iron agent regulation and control. According to the method, the iron agent is applied to the polluted rice field soil to induce the soil-rhizosphere interface to form an iron film, heavy metals (such as As, Cu, Pb and Cd) are fixed by virtue of adsorption and complexing effects of the iron agent, and migration of the heavy metals to rice roots is reduced, so that accumulation of the heavy metals at edible parts (leaves and ears) of the rice is reduced; the tolerance of the rice in a polluted environment is enhanced; and the risk of heavy metals in a food chain is reduced. Experiments show; through treatment of an iron film formed by a certain iron-based concentration, accumulation of the heavy metals in the rice leaves can be remarkably reduced, and the accumulation amount of the heavy metals in the rice leaves is far lower than the accumulation amount (1 / 40-1 / 120) of the heavy metals in rice roots. The invention provides an effective solution for farmland soil remediation and crop safety production.

The invention discloses an application of an OsSPL14 gene and a protein coded by the OsSPL14 gene in regulating and controlling the diameter of a plant root system, and belongs to the technical field of gene engineering. The technical problem to be solved by the invention is how to regulate and control the root diameter of a plant. In order to solve the technical problem, the invention provides application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the diameter of a plant root system. The protein has an amino acid sequence of sequence 2. The invention discloses that OsSPL14 is an important gene for regulating and controlling the diameter of the rice root for the first time. The expression of the OsSPL14 is driven by using a specific promoter, so that the regulation effect of the OsSPL14 in a rice root system can be specifically exerted, and the diameter of the adventitious root of the rice is increased. The OsSPL14 provided by the invention has important significance in cultivating a new rice variety with high and stable yield, high quality and good stress resistance.

The invention provides bacillus mycoides Y2 with functions of reducing cadmium content of crops and promoting growth and application of the bacillus mycoides Y2, and relates to the technical field of soil heavy metal pollution bioremediation and agricultural microorganism application. The bacillus mycoides Y2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 14, 2025, and the preservation number is CGMCC 35231. The strain can be applied to cadmium-polluted soil through root application, can reduce the cadmium content of edible parts of crops such as rice, oilseed rape and fast vegetables, and promotes crop growth and rice root surface iron plaque formation. The contaminated soil in Hunan, Henan and Tianjin is verified to be capable of stably colonizing and adapting to different soils and crops. The strain and the matched preparation are green, efficient, high in stability and wide in universality, and provide key technical support for safe utilization of cadmium-polluted cultivated land.

The invention belongs to the technical field of rice seedling raising devices, and particularly discloses a cadmium-reducing rice seedling raising device, which comprises a seedling raising tray, a plurality of seedling raising grids and a plurality of seedling raising trays, the bottom plate is detachably connected to the bottom of the seedling raising plate, and a plurality of water inlet holes are evenly formed in the positions, right opposite to the seedling raising grids, of the bottom plate. And the dredging device comprises a base, an inserting rod and a liquid pressing assembly, the base is horizontally arranged at the bottom of the bottom plate, and a plurality of supporting assemblies are symmetrically arranged between the edge position of the top of the base and the bottom plate. By arranging the dredging device, when a rice root system or a culture medium in the seedling raising plate blocks the water inlet holes of the bottom plate, a worker can press the seedling raising plate downwards, at the moment, the insertion rods can be accurately inserted into the corresponding water inlet holes, blockages are removed through the physical insertion effect, and the water passing and ventilation functions of the water inlet holes are rapidly recovered; the rice root system is always in an aerobic environment, the root rotting phenomenon is effectively reduced, and the seedling survival rate is improved.

The utility model relates to the technical field of observation devices, in particular to a rice root growth observation device which comprises a planting box and a water tank. The lower end of the planting box communicates with a water tank. According to the utility model, the adjusting plate is inserted into rice for hydroponic planting, so that the root system of the rice is completely exposed in a culture solution and is protected from being blocked and extruded by soil particles, branches, root hair forms and growth directions of the root system can be clearly observed by combining with the transparent window, and subsequent image acquisition by an optical imaging head is facilitated; subsequent analysis and research are facilitated, and the accuracy of image acquisition is improved; when the rice root system is planted and observed through the rolling plate, sunlight can be adjusted to penetrate through the transparent window, the influence of long-time illumination on the root system is avoided, the root system can grow under the appropriate illumination condition, and the experiment accuracy is improved.

The invention discloses a strain of parateratomycosis aveosphaeroides and application thereof, and belongs to the technical field of microorganism and biological prevention and control. In order to screen new species of fungi and provide alternative strains for application of plant beneficial bacteria in agriculture, a fungus SGSF1293 is separated, and the fungus SGSF1293 is identified to be a new species of the cystis clavatum and is named as the parateratosis aveoensis, and the new species of the cystis clavatum is named as the parateratosis aveoensis. According to the present invention, the activity research results show that the strain SGSF1293 has rice root-knot nematode killing activity, plant pathogen resistance (Brassica oleracea black rot xanthomonas, Pseudomonas syringae, Ralstonia solanacearum and Fusarium oxysporum) resistance, oxidation resistance and phosphorus solubilizing activity; besides, the secondary metabolite of the strain is separated and identified, two active compounds, namely, wintergreen ambrotic acid and brefeldin A, are separated, and the wintergreen ambrotic acid has the activity of killing rice root-knot nematode. The discovery of the strain SGSF1293 and the active metabolite thereof has certain application value in the agricultural fields of plant disease control and the like.

The application discloses application of a rice metal tolerance protein coding gene OsMTP7 The rice metal tolerance protein coding gene OsMTP7 has the accession number of AK241225.1 in Genbank. The gene coding protein has zinc transport activity, can increase the zinc content of rice root system and above-ground part, and significantly increases the accumulation of zinc in rice grains.

The invention discloses a compound fertilizer containing traditional Chinese medicine components and application of the compound fertilizer in prevention and treatment of rice sheath blight disease, and belongs to the technical field of fertilizer preparation. The compound fertilizer containing the traditional Chinese medicine components is prepared from the following raw materials in parts by mass: 3 to 5 parts of radix puerariae, 4 to 6 parts of onion, 2 to 3 parts of herba menthae, 2 to 3 parts of flos chrysanthemi indici, 20 to 30 parts of humic acid, 1 to 1.5 parts of compound amino acid, 2 to 3 parts of microbial agent, 12 to 15 parts of soybean meal, 5 to 8 parts of silicon fertilizer, 10 to 15 parts of phosphate fertilizer and 30 to 40 parts of straw fermentation product. The compound fertilizer containing the traditional Chinese medicine components can effectively prevent and treat rice sheath blight disease, stimulate rice growth, improve rice seedling quality, develop rice root systems and improve rice tillering amount, thereby improving disease resistance and yield of rice.

The invention discloses a gene OsARF1 for regulating and controlling the ability of a rice root system to penetrate through hard soil and application of the gene OsARF1. The amino acid sequence coded by the gene OsARF1 is as shown in SEQ ID No. 2. The nucleotide sequence of the gene OsARF1 is as shown in SEQ ID No. 1 (sequence identifier number 1). According to the invention, the OsARF1 gene is over-expressed in rice, so that the expression level of the OsARF1 gene in a rice variety is improved, and then a rice strain with enhanced root penetrating power in hard soil is obtained. The expression of the OsARF1 gene is up-regulated under the condition of hard soil, the expression of the downstream OsCESA6 gene is inhibited, the cellulose synthesis and cell wall thickening of a cortical cell wall are reduced, the thickening of a root system is promoted, and the ability of the root system penetrating through the hard soil is improved. The application of the invention can be used for cultivating a new rice variety suitable for growing in hard soil, and has important significance for improving rice planting under poor soil conditions such as saline-alkali soil.

The invention discloses a nano zeolite coupled biomass charcoal-based slow release fertilizer suitable for acidic rice soil, a preparation method and application, and aims to solve the problems of low pH value, high methane emission flux, low nutrient utilization rate and the like of the acidic rice soil. By constructing a nano zeolite-biomass charcoal-slow release fertilizer-silicon fertilizer synergistic regulation system, the stability of organic carbon in soil and the slow release capacity of nutrients are improved by utilizing a composite material formed by embedding nano zeolite into pores of biomass charcoal; the alkalinity and physiological functions of the silicon fertilizer are used for improving the soil pH and the oxygen secreting capacity of the rice root system, and cooperative emission reduction is achieved through the two paths of inhibiting methane generation and promoting methane oxidation. Field test results show that the pH value of acidic rice soil can be increased by 0.5-1.5 units in a rice growing season, the accumulated emission of methane is reduced by 27%-40%, and the rice yield is increased by 12%-20%. The invention has the advantages of easily available raw materials, simple process, good operability and the like, and can realize the synergistic effect of acid soil improvement, carbon sequestration and emission reduction and crop yield and efficiency increase.

The application provides a strain of Rhodococcus ZJUHULJT1 and application of the strain in prevention and treatment of paratyphaceae root-knot nematodes, and belongs to the technical field of biological prevention and treatment of root-knot nematodes. Rhodococcus The Rhodococcus (sp.) ZJUHULJT1 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251182; the Rhodococcus ZJUHULJT1 can significantly inhibit the infection and reproduction of root-knot nematodes, reduce the number of rice root knots, promote the synthesis of defense-related hormones of rice roots, and improve the disease resistance of rice; through the multiple inhibition of paratyphaceae root-knot nematodes, the Rhodococcus ZJUHULJT1 can be applied to the prevention and treatment of rice root-knot nematodes, has good prevention and treatment effect, and provides a new technical means for the pollution-free reduction of paratyphaceae root-knot nematode diseases.

The invention relates to the technical field of rice stress-resistant cultivation, and discloses a rice root system salt tolerance adjusting method based on exogenous spermidine, which comprises the following core steps: in the critical period of rice root system development, directionally applying a spermidine solution subjected to purity verification to a rice root system area, by accurately regulating and controlling the application concentration, frequency, duration and application environment conditions of spermidine, the osmotic regulation capability and the anti-oxidation system function of a rice root system in a high-salt environment with the soil conductivity larger than or equal to 2 dS / m are improved, the salt tolerance of the root system is remarkably improved, and then growth and development of rice are guaranteed. The spermidine is a straight-chain polyamine compound with good biocompatibility, the chemical purity is not lower than 98%, no heavy metal is left, and the contents of lead, cadmium and mercury are all smaller than or equal to 0.1 mg / kg. According to the invention, precise targeted regulation and control of the salt tolerance of the rice root system are realized, and the regulation effect is stable and remarkable.

This invention provides a non-destructive detection method for rice tillering angle based on YOLO11, with the following steps: (1) Input a frontal image of rice in the tillering stage acquired under natural light; (2) Perform light homogenization processing on the input image; (3) Construct and train a rice tillering key point detection model based on YOLO11-Pose; (4) Detect tillering key points using the trained YOLO11-Pose detection model and form two tillering feature lines; (5) Calculate the angle between the two tillering feature lines, which is the rice tillering angle, and visualize the tillering angle. The YOLO11-Pose detection model construction process includes training data acquisition and preprocessing, model architecture construction, training parameter setting, model training, and model validation. This invention relates to a non-destructive testing method for rice tillering angle based on YOLO11. This method does not damage the original rice root and stem angle during the measurement process and can complete non-contact tillering angle measurement under different lighting conditions, thereby improving the efficiency of tillering angle measurement.

The application of Microbispora longispora in simultaneously enhancing the lodging resistance and tillering ability of rice belongs to the field of biological bacterial manure and rice planting technology. In order to solve the technical problems that the existing technology lacks an effective microbial technical solution capable of simultaneously solving the requirements of'strong stem' and 'tillering' of rice, the seed soaking and inoculation method cannot be compatible with the conventional seed treatment of bactericidal agents, the effective period is short, and the application effect of Microbispora in actual agricultural production is seriously restricted, the application of Microbispora longispora in simultaneously enhancing the lodging resistance and tillering ability of rice is provided, and specifically, the rice seedbed soil is treated by Microbispora longispora, more contact opportunities and time are given to Microbispora and rice roots, the conventional seed bactericide can be compatible, the lodging resistance and tillering ability of rice can be simultaneously enhanced, and the yield of rice is improved.

The utility model discloses an agricultural robot, including 2 walking devices and at least 1 loading mechanism, and the loading mechanism includes loading platform and support mechanism, and each walking device includes a spiral advancing mechanism and at least one motor, and the spiral advancing mechanism is the cylindrical structure that is provided with the external thread outside, and each spiral advancing mechanism is connected with at least one motor, including at least one battery, and the physical axis of 2 spiral advancing mechanisms is parallel to each other, and the rotation direction of the external thread of 2 spiral advancing mechanisms set up on 2 walking devices is opposite, through to the specific setting of walking device, has realized the agricultural robot for rice field this specific use environment can prevent the injury rice root, can scatter the soil structure in the process of rotating and advancing, cut off the weed root system, increases the oxygen content and the air permeability of soil simultaneously, promotes crop root system respiration and growth, makes this agricultural robot can be completely efficiently used in the rice field.

This useful solution addresses the technical process of rice cultivation, focusing on establishing a uniform nutrient environment throughout the entire soil layer before planting. Specifically, it involves applying the entire prescribed amount of inorganic fertilizer for the entire crop, combined with soil preparation to evenly mix fertilizer and straw into the cultivated soil layer before planting. This creates a safe and favorable nutrient environment for the rice root system to grow and develop throughout its cycle, helping to improve yield, quality, efficiency, and reduce environmental pollution, contributing to sustainable agricultural development. This solution does not conflict with other cultivation practices such as: new varieties, mechanization, irrigation, and planting seasons. Therefore, this solution can be applied to the entire rice growing area of ​​Vietnam (7 million hectares / year) and worldwide (approximately 170 million hectares / year). In addition, the technical principles of the solution can be applied to some other short-term crops with similar effectiveness.

The application discloses a rice OsRDR5 gene and a coding protein, a site-directed editing system, application and an application method, a nucleotide sequence of the OsRDR5 gene is shown as SEQ ID NO. 1; and an amino acid sequence of the coding protein of the rice OsRDR5 gene is shown as SEQ ID NO. 2. The gene has a Coa3_cc domain and belongs to a cytochrome c oxidase assembly factor 3 gene family. Knockout mutants of the OsRDR5 gene show a higher deep root ratio, and simultaneously change the gravitropism of rice root systems. The rice OsRDR5 gene of the application regulates the drought avoidance of rice, the knockout mutants have a higher deep root ratio, and can be applied in breeding of new water-saving and drought-resistant rice varieties, so that the drought resistance of rice is improved.

The present application relates to the technical field of rice salt tolerance identification, and discloses a rice seedling stage root length non-damage rapid detection salt tolerance identification device, which comprises an identification tank, the front of the identification tank is a transparent observation window, a culture floating plate is movably arranged in the middle of the identification tank, culture openings are uniformly arranged in the middle of the culture floating plate, culture nets are fixedly installed at the bottom of the culture openings, support bases are fixedly connected to the two sides of the bottom of the identification tank, and movable tracks are fixedly connected to the front and back of the middle of the support bases. The movable supporting plate is additionally arranged at the bottom of the identification tank, and the rotating shaft magnetic strip on the movable supporting plate drives the rotation of the rotor in the identification tank to form a water vortex, so that a downward pulling force is formed on the center line of the vortex, the rice root system at the bottom of the culture opening is gathered and stretched, and the experimenter can directly observe the growth length of the rice seedling root system, thereby avoiding the complicated operation of root length measurement and damage to the rice seedling root system.

The application relates to the technical field of monitoring of salt-tolerant root activity of rice seedlings, and discloses a convenient monitoring device for salt-tolerant root activity of rice seedlings, which comprises a culture pool and a detection pool, the middle of the culture pool is fixedly connected with a partition frame, the top of the partition frame is fixedly connected with a water-proof ring, the water-proof ring is movably placed above a culture plate, five groups of culture openings are formed in the left and right sides of the culture plate, culture nets are fixedly installed in the middle of the culture openings, and the bottom of the culture plate is movably connected with a separate auxiliary plate. The application utilizes the adsorption capacity of the surface of rice root systems, obtains monitoring and recording data of root activity of the same group of rice seeds at different stages under the premise that irreversible damage is not caused to the rice root systems, the isolation barrels on the separate auxiliary plates are used to isolate the rice root systems, the root systems are prevented from being entangled, root activity data of multiple groups of rice seeds is obtained at one time, and the monitoring efficiency is greatly improved.

This invention belongs to the field of biotechnology and relates to a... OsFBX148 Application of genes in regulating plant cold tolerance. This invention reveals a gene in rice called... OsFBX148 Novel functions of the gene and its encoded protein were discovered. Tissue-specific expression analysis showed that the gene was highly expressed in rice roots, and its expression level was significantly upregulated by low-temperature stress. Targeted mutation was performed using gene editing technologies (such as CRISPR / Cas9). OsFBX148 The gene can significantly enhance the cold resistance of rice plants, indicating that the gene plays an important role in regulating the cold resistance of rice. This invention provides an important gene resource for the genetic improvement of cold resistance in rice, and has important application value for breeding crop varieties with enhanced cold resistance and ensuring food security in high-altitude and cold regions.

The invention discloses a sphingomonas strain capable of degrading cellulose, promoting growth and relieving NaCl stress of plants and application of the sphingomonas strain. The invention relates to the field of microorganisms, and provides sphingomonas sphingomonas or a progeny thereof, the sphingomonas sphingomonas is rice root sphingomonas sphingomonas, the strain number of the sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas The strain RS6 provided by the invention is a new strain of sphingomonas, and has the capability of degrading cellulose. A pot experiment shows that compared with a control group without inoculation, the overground fresh weight and underground fresh weight of rice can be improved after the strain is inoculated under the stress of NaCl, and the strain can be used as a microbial organic fertilizer for improving soil fertility, relieving the NaCl stress of crops and improving the adaptability of the crops under the stress of the NaCl.

This invention discloses a method for regulating root silicon deposition and stress resistance based on gene overexpression and silicon supply strategies. This invention is the first to discover a gene that plays a key role in silicon deposition in rice roots. Overexpression of this gene alone or silicon supply alone is not very effective for non-silicon-accumulating plants, but the gene overexpression + silicon supply strategy of this invention can significantly improve the resistance of non-silicon-accumulating plants to heavy metals (such as hexavalent chromium and cadmium) and high-temperature stress.

The invention discloses a chitin leaf fertilizer based on rice cultivation and a preparation method thereof, and relates to the technical field of rice leaf fertilizers. The chitin foliar fertilizer can stimulate the autoimmune system of rice and significantly reduce the incidence rate of fungal and bacterial diseases such as rice blast, banded sclerotial blight and bacterial blight, the molecular structure of the chitin foliar fertilizer can have a certain repelling effect on pests such as aphids and plant hoppers, meanwhile, the toughness of rice skin is enhanced, and the harm of pest gnawing is reduced; the tolerance of the rice to abiotic stress such as drought, low temperature, salt and alkali is improved; in a drought environment, the chitin foliar fertilizer can reduce water transpiration, maintain normal physiological functions, stimulate growth of rice roots, increase root quantity and root hair quantity, improve water and fertilizer absorption capability of the roots, promote tillering and straw strengthening, reduce lodging risk, promote grouting, improve maturing rate and thousand seed weight, and improve rice quality.

The invention relates to application of yellow microspora to simultaneous enhancement of lodging resistance and tillering resistance of rice, and belongs to the technical field of biological bacterial fertilizers and rice planting. The technical problems that in the prior art, an effective microbial technical scheme capable of integrally meeting the requirements of'stalk strengthening 'and'tiller increasing' of rice is lacked, a seed soaking and inoculating method cannot be compatible with a conventional bactericide seed treatment agriculture technology, the effective action period is short, and the application effect of yellow microspora fungi in actual agricultural production is seriously restricted are solved. The invention provides application of yellow microspora to simultaneously enhancing lodging resistance and tillering resistance of rice, and particularly relates to application of yellow microspora to treating rice seedbed soil by utilizing yellow microspora, providing more opportunities and time for contact between yellow microspora and rice roots, being compatible with conventional seed bactericides, and being beneficial to improving the lodging resistance and tillering resistance of rice. And the lodging resistance and tillering resistance of the rice can be synchronously enhanced, so that the yield of the rice is increased.

The invention discloses a traditional Chinese medicine composite agent, a preparation method and a rice isolation reducing method, and relates to the technical field of agricultural planting. The composition is used for reducing cadmium in rice and comprises ginkgo leaves, radix puerariae, radix scutellariae, radix astragali, radix bupleuri, radix sophorae flavescentis, rhizoma coptidis and cortex phellodendri. Wherein the folium ginkgo, the radix puerariae and the radix scutellariae are used for improving the oxidation resistance of the rice; the astragalus membranaceus and the radix bupleuri are used for forming a protective isolation layer at the rhizosphere of the rice; effective components of various traditional Chinese medicines are used for directly blocking cadmium absorption of rice roots, a protective isolation layer is formed at rice rhizosphere, and cadmium absorption of the rice roots is inhibited. The problem of cadmium absorption of the rice is fundamentally solved from the source, so that the effect of reducing cadmium in the rice is achieved.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating cancer-related fatigue, which is prepared from astragalus mongholicus, ginseng, dogwood, lucid ganoderma, herba epimedii, medlar, radix ophiopogonis, schisandra chinensis, glutinous rice root, light wheat, oyster, paris polyphylla, turmeric, salvia miltiorrhiza and scorpion. According to the composition, the core pathogenesis of great injury of primordial qi, deficiency of spleen and kidney and internal retention of residual toxins after prostatic cancer (ADT) is subjected to androgen deprivation treatment, the composition is mainly used for reinforcing primordial qi, replenishing qi, strengthening the spleen and tonifying the kidney, is also used for promoting blood circulation and removing toxins, and can remarkably improve fatigue symptoms, life quality and traditional Chinese medicine clinical syndromes of ADT-related cancer-related fatigue patients. The invention further provides a preparation method of the composition and application of the composition in preparation of drugs for treating cancer-related fatigue, and the composition is good in safety and has important clinical application value.

This invention provides a black hair dye based on plant extracts and its preparation method. Effective components are extracted from plants such as rice roots, ginkgo leaves, arborvitae, rhubarb, cockscomb, chrysanthemum, mulberry, black tea, green tea, gentian, lichen, and catechu. It contains no harmful chemicals such as common paraphenylenediamine or ammonia. This hair dye enables even coloring of hair. The surfactant reduces the surface tension of the dye system, allowing the plant extracts and other components to disperse more evenly in the solution and better contact with the hair. During the dyeing process, it ensures that each hair strand fully absorbs the dyeing components, achieving a uniform coloring effect and avoiding uneven coloring. The color produced by this invention is natural and long-lasting, which is closely related to the synergistic effect of the plant extracts and the mordant ferrous sulfate. The hair dye of this invention performs better in maintaining color stability, allowing consumers to maintain their ideal hair color for a longer period.

This invention discloses a plant root-immersion method based on magnetite nanoparticles and its application, belonging to the field of agricultural biotechnology. Magnetite nanoparticles, or surface-modified magnetite nanoparticles, are used as the root-immersion treatment agent. During the rice seedling stage, the roots are briefly immersed in the nanoparticles, causing them to form a stable "artificial iron film" interface on the rice root surface. Rice seedlings treated with this method show significantly enhanced adsorption and retention capacity of arsenic on the root surface under arsenic concentrations of 0.1–0.5 mg / L. Furthermore, by regulating arsenic speciation and inhibiting arsenic-related transport processes, the accumulation of arsenic in rice roots and leaves is reduced. This invention innovatively employs magnetite nanoparticle root-immersion, significantly enhancing the adaptability of rice to arsenic environments. The method is simple and efficient, and has significant implications for the application of nanomaterials in the control of heavy metal pollution in crops.

The invention discloses a compound amino acid fertilizer and application thereof in preventing and controlling cadmium absorption of rice. The compound amino acid fertilizer comprises amino acid waste mother liquor powder and at least one of molasses and hydrocarbon paraffin. The compound amino acid fertilizer cooperates with the planting density to prevent and control rice plants from absorbing Cd, and the content of Cd in rice roots, stems, leaves, polished rice and brown rice is remarkably reduced. According to the compound amino acid fertilizer, the proportion of a weak acid soluble state and a reducible state of Cd can be reduced, and the proportion of a stable state is relatively increased. According to the compound amino acid fertilizer, the content of available Cd in soil can be remarkably reduced, the maximum reduction value of the available Cd reaches 0.1 mg / kg, and finally absorption of rice to combined pollution heavy metal is reduced; meanwhile, the root system density can be increased. By adopting the compound amino acid fertilizer to cooperate with planting density treatment, safe planting of rice is realized.

This invention discloses the application of the protein OsDIF in regulating drought resistance and root colonization of *Azotobacter brasiliensis* in rice. The amino acid sequence of the protein OsDIF is shown in SEQ ID No. 1. Experiments have demonstrated that it improves homozygous mutant strains. Osdif‑1 The expression level of the protein OsDIF can improve drought resistance and root colonization of *Azotobacter brasiliensis*; while using a protein containing... OsDIF Recombinant Agrobacterium with a gene knockout vector transformed the rice variety Ishikari Shiro-mae, enabling it to... OsDIF Gene editing occurs when two homologous chromosomes... OsDIF When all genes mutate, the activity of the protein OsDIF is lost; this loss of OsDIF activity results in transgenic rice with reduced root colonization of *Azotomyces brasiliensis* and decreased drought resistance. Therefore, the protein OsDIF can regulate drought resistance and root colonization of *Azotomyces brasiliensis* in rice. This invention has significant application value.