38 results about "Bacillus mycoides" patented technology

The invention provides a special charcoal-based slow-release fertilizer for sandy soil as well as a preparation method and application thereof, and belongs to the technical field of fertilizers. The special charcoal-based slow-release fertilizer for the sandy soil is prepared from amino modified biomass charcoal, urea, urea formaldehyde, monoammonium phosphate, potassium sulfate, polyglutamic acid, fulvic acid, clay, attapulgite, a compound microbial agent and sodium alginate. The compound microbial agent is prepared from halomonas (ACCC 11681), bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094), and the compound microbial agent is prepared from bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094). The invention further provides a preparation method and application of the special charcoal-based slow-release fertilizer for the sandy soil. The special charcoal-based slow-release fertilizer for the sandy soil can remarkably improve the water and fertilizer retention capacity of the sandy soil, improve the farmland productivity of the sandy soil, save fertilizer and water input and improve the yield and quality of crops.

The invention discloses a multifunctional low-temperature corn straw degradation complex microbial inoculant, a preparation method and application, the complex microbial inoculant is compounded by a plurality of microorganisms with straw degradation, potassium dissolving and growth promoting functions, and the complex microbial inoculant comprises the following strains: bacillus subtilis K15, curveolate Prickia L11 and bacillus mycoides L31. The complex microbial inoculant disclosed by the invention realizes synergistic interaction and functional complementation, and through scientific compounding of strains, the straw decomposition rate is remarkably increased, and the decomposition period is obviously shortened; the adaptive capacity and colonization competitiveness of microorganisms in a low-temperature and complex soil environment in winter are enhanced. The complex microbial inoculant disclosed by the invention also has multiple functions of degrading straws, dissolving potassium, producing IAA (Indoleacetic Acid) and the like. The soil potassium which is difficult to utilize can be converted into quick-acting potassium while the straws are quickly decomposed to release nutrients.

The application discloses a kind of multifunctional low-temperature degradation corn stalk composite microbial inoculant, preparation method and application, the composite microbial inoculant is compounded by multiple microorganisms with straw degradation, potassium solution and promoting function, comprising the following strains: bacillus subtilis K15, curvularia geniculata L11 and bacillus mycoides L31.The composite microbial inoculant constructed in the application realizes synergistic effect and functional complementation, through strain scientific compounding, straw decomposition rate is significantly improved, and decomposition period is obviously shortened;Enhance the adaptability and colonization competitiveness of microorganisms in winter low temperature and complex soil environment.The composite microbial inoculant of the application also has multiple functions such as straw degradation, potassium solution and IAA production, etc.At the same time of releasing nutrients by rapid decomposition of straw, it can convert difficult-to-use soil potassium into available potassium.

The invention discloses an aflatoxin B1 degrading bacterium and application thereof, and belongs to the technical field of biodegradation. The name of the degrading bacterium is bacillus paramycoides HS-1, and the preservation number of the degrading bacterium is GDMCC No: 66506. The invention further discloses a preparation method of the degrading bacterium. After the strain is cultured in an LB broth culture medium containing 2 [mu] g / mL of AFB1 for 16 h, the degradation rate of the AFB1 can reach 35% or above. The heat stability of the fermentation supernatant filtrate of the HS-1 strain for degrading the AFB1 is good, after the fermentation supernatant filtrate is treated in a boiling water bath at the temperature of 100 DEG C for 20 min, the 48-hour degradation rate of 2 microgram / mL AFB1 can be stabilized to be 70% or above, and 2 microgram / mL AFB1 can be completely degraded at the temperature of 70 DEG C, the pH value of 11 and 48 hours.

The invention provides bacillus mycoides Y2 with functions of reducing cadmium content of crops and promoting growth and application of the bacillus mycoides Y2, and relates to the technical field of soil heavy metal pollution bioremediation and agricultural microorganism application. The bacillus mycoides Y2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 14, 2025, and the preservation number is CGMCC 35231. The strain can be applied to cadmium-polluted soil through root application, can reduce the cadmium content of edible parts of crops such as rice, oilseed rape and fast vegetables, and promotes crop growth and rice root surface iron plaque formation. The contaminated soil in Hunan, Henan and Tianjin is verified to be capable of stably colonizing and adapting to different soils and crops. The strain and the matched preparation are green, efficient, high in stability and wide in universality, and provide key technical support for safe utilization of cadmium-polluted cultivated land.

This invention discloses a strain of *Bacillus paramycoides* and its application in selenium oxidation, belonging to the field of agricultural microbiology. A strain of *Bacillus paramycoides* H1 was isolated from the soil of a selenium-nutrition experimental field at Huazhong Agricultural University and deposited at the China Center for Type Culture Collection (CCTCC) on June 27, 2023, with accession number CCTCCNO: M 20231107. Experimental verification showed that this strain can simultaneously oxidize elemental selenium and organic selenium. This invention utilizes this strain to convert organic and elemental selenium into water-soluble selenium that promotes plant absorption, laying a scientific foundation for the preparation of safe and low-cost plant selenium-enriching agents and selenium oxidants. This is of great significance for the research and agricultural cultivation of selenium conversion model strains.

The invention discloses a compound microbial fertilizer suitable for flue-cured tobacco production as well as a preparation method and application thereof. The compound microbial fertilizer is prepared from the following components in percentage by mass: 4-8% of a functional microbial agent, 12-18% of a first modifier, 1-2% of a second modifier, 30-70% of an inorganic material and the balance of pure water, wherein the first modifier is a polyglutamic acid stock solution in which the mass percent of polyglutamic acid is greater than or equal to 3.5%, and the second modifier is a gamma-aminobutyric acid stock solution in which the mass percent of gamma-aminobutyric acid is greater than or equal to 50%; the functional microbial agent is prepared by compounding a bacterial strain XNSD01 and a bacterial strain XNSD02, the bacterial strain XNSD01 is bacillus siamensis with the preservation number of CCTCC (China Center for Type Culture Collection) NO.M 20252934, and the bacterial strain XNSD02 is bacillus mycoides with the preservation number of CCTCC NO.M 20252935. And unification of disease resistance, growth promotion and successive cropping obstacle alleviation is realized.

The present application relates to the field of bio-agriculture, and particularly relates to a bio-agricultural compound microbial fertilizer and a preparation method thereof, which comprises, by weight, 2-8 parts of functional microbial flora, 20-48 parts of organic carrier, 5-20 parts of nutrient component, 0.5-4 parts of microbial body protective agent, and the balance of water; the functional microbial flora is composed of Pseudomonas fluorescens, Trichoderma harzianum, Saccharomyces cerevisiae and Bacillus mycoides; and the organic carrier is composed of a peanut shell-sugarcane residue mixture, modified diatomite and humic acid. The present application forms a complete compound microbial fertilizer technical solution through scientific compounding of multiple strains, directional modification of the organic carrier, balanced optimization of the nutrient component and low-temperature preparation process synergy, and has multiple outstanding beneficial effects.

The present application relates to the technical field of bio-organic fertilizer preparation, and particularly relates to a bio-organic fertilizer prepared by microbial fermentation and a preparation method thereof, which is prepared by fermentation of raw materials and compound microbial strains, wherein the raw materials include livestock and poultry manure, crop straw, industrial organic waste residue and a humification agent, and the compound microbial strains are a ternary combination of bacillus subtilis, bacillus mycoides and saccharomyces cerevisiae, or a quaternary combination of bacillus subtilis, bacillus mycoides, saccharomyces cerevisiae and bacillus mucilaginosus. The present application adopts a multi-element compound strain combination and optimizes the ratio, and the synergistic effect of each strain is significant, which greatly improves the organic matter degradation efficiency and effective nutrient activation capacity, and compared with single strain, the microbial activity is stronger, and the nutrient supply capacity of the fertilizer is better.

The invention discloses a bio-based fertilizer and application thereof. The preparation method comprises the following steps: S1, crushing the mango pomace and the bagasse, sieving the crushed mango pomace and bagasse with a 40-60-mesh sieve, adding diluted hydrochloric acid and ferrous sulfate, mixing the crushed mango pomace and bagasse, carrying out a hydrolysis reaction, and filtering a hydrolysis product to obtain solid residues; s2, seed liquid of bacillus coagulans, bacillus pseudomycoides, trichoderma viride and saccharomyces cerevisiae is inoculated into the mixture of the solid residues and the cattle and sheep manure for fermentation, and a fermentation product is obtained; s3, mixing the fermentation product, charcoal and polyglutamic acid to prepare the fertilizer. The mango pomace, solid bagasse and cattle and sheep manure mixture are cooperatively fermented through compound bacteria, the porosity of the fertilizer is increased, the water and fertilizer retention capacity is improved, the fermentation product, biochar and polyglutamic acid are mixed and granulated to prepare the fertilizer, the disintegration rate can be regulated and controlled, nutrients are prevented from being instantly released, and root growth and microorganism colonization are promoted.

The invention relates to the technical field of organic fertilizers, and particularly discloses a crop organic fertilizer and a preparation method thereof.The organic fertilizer is prepared from, by weight, 45%-50% of castor cake meal, 20%-22% of rice bran oil meal, 10%-15% of sesame cake, 2%-3% of molasses, 5%-10% of seaweed extract powder, 2%-5% of humic acid and 5%-6% of a compound microbial agent. The compound microbial agent comprises bacillus subtilis, bacillus mucilaginosus, bacillus megaterium and saccharomyces cerevisiae, industrial processing by-products are used as main raw materials, efficient resource utilization of agricultural waste is achieved, environmental pollution caused by waste incineration or random discarding is reduced, the environment is protected, and the environment is protected. Meanwhile, after the fertilizer is used for a long time, the growth condition of crops can be remarkably improved, the use amount of pesticides is reduced, the pesticide residue risk of agricultural products is reduced, and the quality safety of the agricultural products is guaranteed.

The invention discloses a macroelement water-soluble fertilizer and production equipment thereof, and relates to the technical field of water-soluble fertilizer production, and the macroelement water-soluble fertilizer comprises, by weight, 25-30 parts of a nitrogen source, 25-30 parts of a phosphorus source, 20-25 parts of a potassium source, 15-20 parts of an anti-hardening agent, 8-15 parts of a soil improvement agent, 0.2-0.5 part of a microbial agent, and 1.2-1.5 parts of trace elements. Wherein the anti-hardening agent can stimulate plant root systems to secrete polysaccharide viscose substances, and soil particles are cemented to form a stable aggregate structure; meanwhile, alginate oligosaccharide in the anti-hardening agent can be matched with bacillus mucilaginosus to promote the bacillus mucilaginosus to secrete polysaccharide and organic acid, so that the stability of soil aggregates can be enhanced, and soil pores are improved; the polyglutamic acid can adsorb calcium and magnesium ions in the soil and reduce insoluble salt formed by the ions, phosphate radicals and sulfate radicals, so that soil hardening is reduced.

This invention provides a strain of Bacillus mycoides Y2 with functions of reducing cadmium content in crops and promoting growth, and its applications, relating to the fields of soil heavy metal pollution bioremediation and agricultural microbial application technology. Bacillus mycoides Y2 was deposited on July 14, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC35231. This strain can be applied to cadmium-contaminated soil via root application, reducing cadmium content in the edible parts of crops such as rice, rapeseed, and kale, and promoting crop growth and the formation of an iron film on the root surface of rice. Verification with contaminated soils in Hunan, Henan, and Tianjin has shown that it can stably colonize and adapt to different soils and crops. This strain and its accompanying formulation are green, efficient, highly stable, and widely applicable, providing key technical support for the safe utilization of cadmium-contaminated arable land.

The application discloses a bacillus mycoides BMS-39 and application thereof, and belongs to the technical field of microorganisms and agricultural biological control. Fulvia fulva The strain BMS-39 has a very strong inhibiting effect, the inhibiting rate reaches 92.3% by means of a flat-plate confrontation method, the inhibiting rate of 10% fermentation filtrate reaches 87.6%, the potting prevention effect reaches 87.01% in 14 days, and the field prevention effect reaches 87.81%. Even when the concentration of the bacterial liquid is reduced to 10 6 CFU / mL, the prevention effect of the BMS-39 can still reach 81.46%, which means that the fermentation cost and application cost of the application can be greatly reduced under the premise of meeting the same or even better prevention effect, and the application has significant market competitiveness.

The invention discloses a construction method of a novel composite biocontrol flora for preventing and treating cotton verticillium wilt, and relates to the technical field of biological prevention and treatment of agricultural plant diseases. The bacillus polymyxa and the bacillus cereus with the optimal antagonistic activity are respectively screened out through plate confrontation and buckling tests, and the bacillus pseudomycoides is obtained through screening by taking the growth promoting capability of dissolving phosphorus and producing indoleacetic acid as indexes. Through pairwise cross culture verification, the three strains are completely compatible and have no mutual inhibition effect. The method comprises the following steps: respectively and independently activating the three strains, carrying out enlarged culture, preparing single bacterial liquid with the OD600 value of 1.0, and uniformly mixing according to a volume ratio, so as to obtain the novel composite biocontrol flora for preventing and treating the cotton verticillium wilt. According to the invention, antagonistic bacillus and growth-promoting bacillus with complementary functions are screened, single bacteria are independently cultured and then are compounded according to a ratio of 1: 1: 1 to construct a composite flora, so that the synergistic interaction of efficient prevention and control of cotton verticillium wilt, cotton growth promotion and soil phosphorus solubilization is realized.

The invention relates to a novel bacterial strain and application thereof. The bacterial strain is bacillus paramycosis KCTC14777BP; the invention also relates to a preparation method of a fermentation product and a preparation method of a fraction comprising the polypeptide. And a cosmetic composition, a foreign medicine composition, or a food composition comprising at least one substance selected from the group consisting of the strain, a culture of the strain, a fermented product of the strain, and a fraction of the culture or the fermented product. A strain of Saccharomyces cerevisiae, Lactobacillus fructovorans, Bacillus paramycosis, or Bacillus megaterium, a culture of the strain, a fermented product of the strain, or a culture or a fraction of the fermented product has excellent effects of improving skin and calming sensitive skin. Therefore, the use ratio of the composition as a skin-improving cosmetic composition, a pharmaceutical composition, and a food composition is high. Therefore, various industrial applications such as cosmetics, foreign drugs and food can be expected.

The invention provides bacillus mycoides and an application of the bacillus mycoides in synthesis of manganese oxide. The bacillus mycoides CP203 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 24208. The invention further provides application of the bacillus mycoides in biosynthesis of manganese oxide, divalent manganese ions in a solution can be oxidized into manganese oxide under the non-contact condition, and the synthesized manganese oxide product does not contain organisms such as thalli and spores; the bivalent manganese ions and other harmful substances in the raw materials do not generate adverse effects on the growth of microorganisms, the purification cost of biosynthetic manganese oxide can be remarkably reduced, and the variety and range of biologically-treatable manganese ion wastewater can be expanded, so that the method has important application in the fields of biosynthesis of manganese oxide, hazardous wastewater treatment and the like.

The invention relates to a novel bacterial strain and application thereof. The bacterial strain is lactobacillus fructovorans KCTC14776BP; the invention also relates to a preparation method of a fermentation product and a preparation method of a fraction comprising the polypeptide. And a cosmetic composition, a foreign medicine composition, or a food composition comprising at least one substance selected from the group consisting of the strain, a culture of the strain, a fermented product of the strain, and a fraction of the culture or the fermented product. A strain of Saccharomyces cerevisiae, Lactobacillus fructovorans, Bacillus paramycosis, or Bacillus megaterium, a culture of the strain, a fermented product of the strain, or a culture or a fraction of the fermented product has excellent effects of improving skin and calming sensitive skin. Therefore, the use ratio of the composition as a skin-improving cosmetic composition, a pharmaceutical composition, and a food composition is high. Therefore, various industrial applications such as cosmetics, foreign drugs and food can be expected.

The application discloses a preparation method of an extractive for preventing and treating citrus anthracnose, and comprises the following steps: drying garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed, and then crushing the dried garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed; adding the crushed garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed into boiling water and stirring until uniform; continuing to cook; freezing; thawing after the freezing is completed; obtaining a freeze-thawed material; adding sodium dihydrogen phosphate into the freeze-thawed material; placing the freeze-thawed material in a fermentation tank; inoculating bacillus subtilis and bacillus mycoides for aerobic fermentation; inoculating bacillus licheniformis, bacillus laterosporus and bacillus thuringiensis for aerobic fermentation; completing the aerobic fermentation; carrying out anaerobic fermentation on the fermented material; stirring the fermented material and pure water until uniform; filtering after standing; taking filter residue and precipitate as extractive A; adjusting the PH value of filtrate to obtain extractive B; adding bagasse powder into the extractive A, compacting, packaging and bagging; and sealing and storing the extractive B. The extractive prepared by the application can efficiently prevent and treat citrus anthracnose, and has the characteristics of low production cost and no pollution to citrus planting environment.

The invention relates to the technical field of planting, in particular to a fungicide and fertilizer suitable for planting landscape plants. The microbial inoculum disclosed by the invention is prepared from the following components in parts by mass: 5 to 10 parts of bacillus coagulans microbial inoculum, 4 to 8 parts of bacillus laterosporus microbial inoculum, 10 to 18 parts of bacillus mucilaginosus microbial inoculum, 6 to 10 parts of bacillus velezensis microbial inoculum, 2 to 6 parts of trichoderma viride, 6 to 10 parts of bacillus amyloliquefaciens microbial inoculum and 2 to 6 parts of paecilomyces lilacinus. Through compatibility of various microbial agents, the soil structure can be improved, and the soil fertility can be improved. The microbial agent disclosed by the invention is prepared into a fertilizer, and the obtained fertilizer also has the effects of regulating the soil microhabitat, improving the soil structure and improving the soil fertility. The growth of garden plant roots can be promoted, and the growth performance of garden plants is improved. And a basis is provided for planting of landscape plants.

The invention relates to the technical field of molecular biology, in particular to a multiplex PCR (polymerase chain reaction) primer group and a reagent for identifying five bacilli and application of the multiplex PCR primer group and the reagent. The primer group comprises BT-F / R, BC-F / R, BL-F / R, BS-F / R and BM-F / R. When the primer group is used for multiple PCR amplification, the five bacilli, namely bacillus thuringiensis, bacillus cereus, bacillus licheniformis, bacillus subtilis and bacillus mycoides, can be simultaneously detected at one time, and the detection is high in specificity, convenient and efficient.

The application relates to the technical field of biological fertilizer, and particularly discloses a compound microbial fertilizer and a preparation method thereof. The Klebsiella pneumoniae, Bacillus megaterium, Bacillus mycoides and Bacillus subtilis are compounded, the Klebsiella pneumoniae converts nitrogen in the air into nitrogen available for plants, the Bacillus megaterium decomposes insoluble phosphorus in soil into effective phosphorus, the Bacillus mycoides decomposes potassium silicate minerals in soil to release effective potassium, the Bacillus subtilis produces biological substances to stimulate root growth and secretes antibacterial substances to inhibit pathogenic bacteria in roots. The modified biochar carrier is used to provide stable living space for the compound microbial fertilizer, so that the compound microbial fertilizer is sustainable, efficient and green.

The invention discloses a special compound microbial inoculant for a natural alpine grassland and a preparation method of the special compound microbial inoculant. The special compound microbial inoculant is prepared from a bacterial strain culture solution of bacillus mycoides Gnyt1, a bacterial strain culture solution of bacillus subtilis GAU-00667, a bacterial strain culture solution of pseudomonas pseudomonascus GAU-00690 and a bacterial strain culture solution of pseudomonas pseudomonascus GAU-00689. The preparation method comprises the following steps: preparing a microbial strain culture solution of bacillus mycoides Gnyt1, a bacterial strain culture solution of bacillus subtilis GAU-00667, a bacterial strain culture solution of pseudomonas pseudomonascus GAU-00690 and a bacterial strain culture solution of pseudomonas pseudomonascus GAU-00690, and preparing a microbial strain culture solution of The compound microbial inoculant provided by the invention has multiple characteristics of nitrogen fixation, phosphorus dissolution, growth hormone secretion and the like, can effectively antagonize pathogenic fungi, can be suitable for improvement of alpine grassland and treatment of degraded alpine grassland, can be used in cooperation with measures such as organic fertilizer, nitrogen, pasture reseeding, turf scratching, fence breeding and the like, and can also be used independently; the seed dressing agent can be used as a pasture seed dressing agent and can also be directly sprayed. The microbial agent not only can increase soil nutrients, but also can efficiently promote the growth of dominant plants. In addition, the microbial agent also has the advantages of being green, environment-friendly, economical, practical and good in continuity.

The application discloses a saline-alkali soil conditioner and a preparation method thereof, and belongs to the technical field of soil improvement. The soil conditioner is prepared from the following raw materials in parts by weight: modified phosphogypsum 100-200 parts, microbial agent 5-10 parts, and activator 10-20 parts; the modified phosphogypsum is prepared from phosphogypsum and modified coal gangue after compounding and rare earth modification; the microbial agent is prepared from Bacillus subtilis, Streptomyces cellulans and Bacillus mycoides. The chemical-physical-biological improvement technology is combined, the physical and chemical properties of the saline-alkali soil are effectively improved, the soil biological activity is increased, the growth of crops is promoted, the yield and disease resistance of crops are improved, and the resource utilization rate of phosphogypsum is improved.

The present application belongs to the technical field of drought resistance of Mesona chinensis Benth, and particularly relates to a microbial agent for improving drought resistance of Mesona chinensis Benth. The microbial agent for improving drought resistance of Mesona chinensis Benth is composed of Bacillus circulans bacterial liquid and Bacillus mycoides bacterial liquid, and the volume ratio of the Bacillus circulans bacterial liquid to the Bacillus mycoides bacterial liquid is 1:3-5. The microbial agent of the present application is composed of the Bacillus circulans bacterial liquid and the Bacillus mycoides bacterial liquid in a volume ratio of 1:3-5, and the Bacillus circulans bacterial liquid and the Bacillus mycoides bacterial liquid are respectively obtained by culturing corresponding strains in a liquid culture medium. During the planting of Mesona chinensis Benth, the microbial agent of the present application can significantly delay the damage to the Mesona chinensis Benth plants under drought stress, and is helpful to improve the drought resistance of the Mesona chinensis Benth, which is of great significance to the development of the Mesona chinensis Benth industry.

The invention relates to a novel bacterial strain and application thereof. The bacterial strain is saccharomyces cerevisiae KCTC14779BP; the invention also relates to a preparation method of a fermentation product and a preparation method of a fraction comprising the polypeptide. And a cosmetic composition, a foreign medicine composition, or a food composition comprising at least one substance selected from the group consisting of the strain, a culture of the strain, a fermented product of the strain, and a fraction of the culture or the fermented product. A strain of Saccharomyces cerevisiae, Lactobacillus fructovorans, Bacillus paramycosis, or Bacillus megaterium, a culture of the strain, a fermented product of the strain, or a culture or a fraction of the fermented product has excellent effects of improving skin and calming sensitive skin. Therefore, the use ratio of the composition as a skin-improving cosmetic composition, a pharmaceutical composition, and a food composition is high. Therefore, various industrial applications such as cosmetics, foreign drugs and food can be expected.

The invention belongs to the technical field of agricultural biology, and particularly discloses a functional seedling culture substrate for preventing and treating root-knot nematode and application of the functional seedling culture substrate. The functional seedling raising substrate is composed of a solid seedling raising substrate and a compound microbial agent uniformly dispersed in the solid seedling raising substrate. The compound microbial agent is prepared by carrying out mixed fermentation on bacillus velezensis RKN1111, bacillus laterosporus CDD-01 and paenibacillus mucilaginosus HX-02. The invention further discloses a preparation method of the compound microbial agent. When the functional seedling culture substrate is used for seedling culture, the colonization rate of the compound microbial agent in seedling rhizosphere is greatly improved, the inside and the outside of root lumps of cultured germchit are rich in active biocontrol bacteria during transplanting, the germchit can continuously play a high-efficiency and stable prevention and control role after transplanting, and the growth of the germchit is remarkably promoted. According to the method, the prevention and control opportunity of the root-knot nematode is greatly advanced from a field period to a controllable seedling raising period, active immunization is achieved, and the technical problems that colonization is difficult and the effect is unstable when a biocontrol microbial inoculum is applied to traditional root irrigation or fields are effectively solved.

The present application provides a bacillus mycoides and its application in synthesizing manganese oxide, and the bacillus mycoides (Bacillus mycoides) CP203 with a preservation number of CGMCC No. 24208 is provided. Bacillus mycoides The present application further provides the application of the aforementioned bacillus mycoides in biosynthesis of manganese oxide, which can oxidize divalent manganese ions in a solution into manganese oxide without contact, the synthesized manganese oxide product does not contain organisms such as bacteria and spores, and the divalent manganese ions and other harmful substances in raw materials will not adversely affect the growth of microorganisms, which can significantly reduce the purification cost of biosynthesis of manganese oxide, expand the types and range of biologically treated manganese ion wastewater, and therefore the present application has important applications in the fields of biosynthesis of manganese oxide and hazardous waste water treatment.

The application discloses high water permeable concrete and a preparation method thereof, and belongs to the technical field of concrete. The high water permeable concrete is prepared from the following raw materials in parts by weight: 1100-1300 parts of coarse aggregate, 320-350 parts of cement, 50-70 parts of reinforcing fiber, 2-4 parts of water reducing agent, 110-120 parts of water, 48-60 parts of fly ash, 46-75 parts of compound microcapsules and 22-32 parts of cementing reinforcing agent. The high water permeable concrete has high water permeability, excellent mechanical strength and durability by the synergistic effect of the compound microcapsules of Bacillus psuedofirmus and Bacillus sphaericus and the cementing reinforcing agent. In addition, the high water permeable concrete is used to prepare water permeable pavement, and has the advantages of convenient construction, quick elimination of the water accumulation phenomenon during raining and effective play of the water permeable concrete.

The invention discloses a method for preparing vegetation planting soil by utilizing industrial solid waste and microorganisms and application of the vegetation planting soil. The method comprises the following steps: mixing 80-90% of high titanium slag, 7-15% of sandy soil or phosphogypsum and 2-5% of titanium gypsum into a vegetation matrix according to specific particle size grading; inoculating a composite strain consisting of bacillus mucilaginosus and azotobacter chroococcum, fermenting at 30 DEG C for 12-18 days, and dynamically monitoring the contents of alkali-hydrolyzable nitrogen, available phosphorus and potassium and biologically extractable titanium; then, alfalfa is planted, arbuscular mycorrhizal fungi and rhizobium suspensions with the OD600 being 0.8-1.0 are regularly irrigated, and curing is conducted for 12-18 days; and irrigating and maintaining for 12-18 days by using chlorella propagation liquid every 4-6 days, and finally reserving the whole plant or harvesting to obtain the plant growing planting soil. According to the method, industrial solid waste is efficiently converted into a functional planting medium through the multi-stage biological-mineral synergistic effect, solid waste recycling and soil function reconstruction are achieved, and the method has the environment restoration and ecological reconstruction value.