46 results about "Bacillus mycoides" patented technology

The invention relates to the field of microorganism, and discloses a microbiological strain for repairing heavy metal contaminated soil, and a screening method and application thereof. The preservation number of the microbiological strain disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 10127. According to the screening method disclosed by the invention, a bacillus mycoides is screened from soil contaminated by heavy metal cadmium, exchangeable cadmium in the soil can be passivated by the bacillus mycoides, the content of the exchangeable cadmium can be obviously reduced, and meanwhile, a very strong tolerance on cadmium is also obtained; disturbance on the soil is small, a stronger biological repairing function is obtained, and the microbiological strain can be applied to repairing of the heavy metal contaminated soil and preparing of heavy metal contaminated repairing materials.

A method of inducing systemic acquired resistance to infection in a plant by applying a composition comprising a Bacillus control agent to the foliage of said plant wherein said plant is capable of producing defense proteins.

The invention provides a method for repairing lead-polluted soil by the combination of plants and microorganisms. The method comprises the following steps: uniformly scattering Indian leaf mustard seeds into lead-containing soil and watering to keep a moist soil condition; and after 10-15 days, adding a microorganism compound bacterial agent into the Indian leaf mustard root soil when the plants sprout and grow, so as to remove lead in the soil by the combination of the absorption effect on the lead of Indian leaf mustards and the strengthening effect of the microorganism compound bacterial agent, wherein the microorganism compound bacterial agent is prepared by mixing Bacillus mycoides, Shewanella and Pseudomonas aeruginosa in a mass ratio of (3-10) to 1 to 1. According to the method, the operation is convenient, the cost is low, the management is easy, secondary pollution cannot be caused, and the application prospect is wide.

The invention discloses forest tree rhizosphere bacteria Bacillus paramycoide s JYZ-SD5 and application thereof. The forest tree rhizosphere bacteria are classified and named as Bacillus paramycoidesJYZ-SD5, and preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO:M2018469. By detecting the biological characteristics of the bacteria JYZ-SD5, the bacteriahave the abilities of nitrogen fixation, phosphorus dissolution, potassium dissolution and IAA production, have an obvious promotion effect on the growth of forest trees, have certain inhibition effects on some forest tree pathogenic fungi, have high heavy metal tolerance, such as Ni2+, Gr6+ and Mn7+, and have a good application prospect.

The invention relates to special mixed strain microbial fertilizer for solanaceous vegetables and a preparation method of the special mixed strain microbial fertilizer and belongs to the technical field of agricultural microorganisms. The special mixed strain microbial fertilizer is characterized by comprising components of raw materials in percentage by weight as follows: 30%-35% of charcoal powder, 25%-30% of peat, 6%-10% of edible fungi residues, 6%-10% of humic acid, 1%-3% of potassium fulvate, 1%-3% of rare earth, 10% of Bacillus mycoides Gnyt1 strain fermentation broth and 10% of Bacillus subtilis FX2 strain fermentation broth. The mixed strain microbial fertilizer has functions of immobilizing nitrogen in air and dissolving indissolvable phosphorus in soil and can supply comprehensive, balanced and absorbable nutrients to the solanaceous vegetables, besides, strains in the microbial fertilizer have capacity of secreting plant hormones and antagonizing pathogenic bacteria, growthof the solanaceous vegetables can be adjusted, and diseases can be controlled.

The invention discloses a solid composite microbial inoculant for promoting aerobic composting of kitchen waste as well as preparation and application of solid composite microbial inoculant. The solid composite microbial inoculant is prepared from bacillus altitudinis, alcaligenes faecalis, lysinibacillus capsici, Bacillus velezensis, alcaligenes aquae, providencia, Bacillus paramycoides, bacillus licheniformis, bacillus thuringiensis, liquid culture media and bran. The solid-state microbial compound inoculant can shorten the temperature rise time of kitchen waste compost and prolong the high-temperature period time, so that the compost can quickly reach the thoroughly decomposed and harmless standards, and the condensation and aromatization of humus are promoted. In addition, the abundance of beneficial bacterium bacillus in the composting process of the kitchen waste is also remarkably improved.

The invention relates to pear rhizosphere growth promoting bacillus mycoides Lzh-Z7 and application thereof. The bacillus mycoides Lzh-Z7 is preserved in the General Microbial Center of the China Committee for Culture Collection of Microorganisms, the preservation number is CGMCC No.13491, the preservation date is September 23rd, 2016, and the address of the preservation institution is No.3 No.1 yard Beichen west road Chaoyang district, Beijing. The invention further discloses application of the bacillus mycoides Lzh-Z7, fermentation liquor and a microbial fertilizer containing the bacillus mycoides Lzh-Z7 in the aspect of preventing and treating pear root rot. Experiments show that the microbial fertilizer prepared from the bacillus mycoides Lzh-Z7 disclosed by the invention can effectively prevent and treat pear brown spot diseases, improve pear quality and yield and has very good application potential.

The invention relates to special mixed-strain microbial fertilizer for onobrychis viciifolia and a preparation method thereof, and belongs to the technical field of agricultural fertilizer. The fertilizer is prepared from charcoal powder, flower cultivating earth, rotten cattle excrement, straw powder, potassium fulvate, rare earth, onobrychis-viciaefolia nitragin, bacillus mycoides fermentation broth, pseudomonas fermentation broth and bacillus subtilis fermentation broth. In addition, the invention also relates to the preparation method of the fertilizer. The preparation method comprises thefollowing steps of preparing a carrier, crushing, sieving, uniformly mixing and sterilizing raw materials; culturing bacterial broth and inoculating on the carrier. The mixed-strain microbial fertilizer has the effects of being capable of providing comprehensive, balanced and easily-absorbed nutrients for the onobrychis viciifolia, preventing the generation of a soil-borne disease on the onobrychis viciifolia, regulating the pH (Potential of Hydrogen) of soil, increasing the fertility of the soil and avoiding an environmental problem caused by the abuse of chemical fertilizer.

The invention discloses Bacillus paramycoides. The nucleotide sequence of the Bacillus paramycoides is shown in SEQ ID No. 1. The invention provides Bacillus paramycoides and the gene sequence of theBacillus paramycoides, in addition, the Bacillus paramycoides is utilized to degrade feathers, the degradation efficiency is high, no pre-treatment of the feathers is needed before degradation, and nopollution to the environment is caused in the degradation process.

The invention relates to a microbial foam extinguishing agent and mainly aims to solve the problem of environmental pollution existing in the prior art. The microbial foam extinguishing agent disclosed by the invention comprises microbes, a surfactant, a cosolvent and an additive, wherein the microbes are bacillus strains separated from soil; the bacillus is at least one of bacillus subtilis, bacillus macerans, bacillus alcalophilus, bacillus polymyxa, bacillus licheniformis, bacillus mycoides, bacillus natto and bacillus amyloliquefaciens; the surfactant is a compound of a hydrocarbon surfactant and a fluorocarbon surfactant and contains at least one fluorocarbon surfactant; the cosolvent is diethylene glycol momobutyl ether or triethylene glycol momobutyl ether; and the additive contains a stabilizing agent, a thickening agent, an anti-corrosive agent and inorganic nutritional substances. The technical scheme of the microbial foam extinguishing agent preferably solves the problems and the microbial foam extinguishing agent can be applied to fire protection and fire extinguishment.

The present invention relates to a composition comprising at least one biological control agent selected from the group consisting of Bacillus chitinosporus AQ746 (NRRL Accession No, B-21618), Bacillus mycoides AQ726 (NRRL Accession No, B-21664), Bacillus pumilus (NRRL Accession No, B-30087), Bacillus pumilus AQ717 (NRRL Accession No, B-21662), Bacillus sp, AQ175 (ATCC Accession No. 55608), Bacillus sp, AQ177 (ATCC Accession No, 55609), Bacillus sp. AQ178 (ATCC Accession No, 53522), Bacillus subtilis AQ743 (NRRL Accession No, B-21665), Bacillus subtilis AQ713 (NRRL Accession No, B- 21661), Bacillus subtilis AQ153 (ATCC Accession No, 55614), Bacillus thuringiensis BD#32 (NRRL Accession No. B-21530), Bacillus thuringiensis AQ52 (NRRL Accession No, B- 21619), Muscodor albus 620 (NRRL Accession No, 30547), Muscodor roseus A3-5 (NRRL Accession No. 30548), Rhodococcus globerulus AQ719 (NRRL Accession No. B-21663), Streptomyces galbus (NRRL Accession No. 30232), Streptomyces sp. (NRRL Accession No. B-30145), Bacillus thuringiensis subspec. kurstaki BMP 123, Bacillus subtilis AQ30002 (NRRL Accession No. B-50421), and Bacillus subtilis AQ 30004 (NRRL Accession No. B-50455) and / or a mutant of these strains having all the identifying characteristics of the respective strain, and / or a metabolite produced by the respective strain that exhibits activity against insects, mites, nematodes and / or phytopathogens and at least one fungicide (I) in a synergistically effective amount, Furthermore, the present invention relates to the use of this composition as well as a method for reducing overall damage of plants and plant parts.

The invention discloses special mixed bacterial fertilizer for umbelliferae. The bacterial fertilizer is prepared from the following raw materials in parts by weight: 25-35 parts of edible mushroom dreg, 15-25 parts of flower cultivating soil, 5-15 parts of charcoal dust, 3-8 parts of potato pulp, 3-8 parts of rotten cow dung, 3-8 parts of turf, 2-5 parts of potassium fulvic acid, 1-3 parts of rare-earth attapulgite, 8-12 parts of bacillus mycoides bacterial fermentation broth and 8-12 parts of bacillus subtilis bacterial fermentation broth. The bacterial fertilizer has the effects of efficiently antagonizing multiple phytopathogens, is environment-friendly, has multiple functions of solubilizing phosphate, fixing nitrogen, secreting plant hormones and the like, has the effects of conditioning soil as well as preventing soil degradation and soil caking, and has low cost.

The invention discloses a bacillus mycoides strain and application thereof, particularly the bacillus mycoides strain WWM09 with a preservation number of CGMCC NO.11047. The invention further discloses a biological control preparation containing the bacillus mycoides strain WWM09 and a preparation method of the biological control preparation. Experiments show that the bacillus mycoides strain WWM09 is large in diameter, resistant to hypoxia, wide in action spectrum and high in adaptability, and can be rapidly grown and multiplied in the low-nutrition state, thereby having a good application prospect; the biological control preparation prepared from the bacillus mycoides strain WWM09 can serve as biological pesticide or bio-fertilizer to control cucumber powdery mildew, grape downy mildew, rose powdery mildew and other leaf diseases, and further to control cotton damping-off, watermelon blight and other soil-borne plant diseases.

The invention discloses a complex microbial inoculant. The complex microbial inoculant comprises, by weight, 30-50 parts of bacillus subtilis fermented liquid, 20-30 parts of bacillus mycoides fermented liquid, 10-20 parts of coral nocardiaceae fermented liquid, 10-20 parts of functional microbial inoculants and 60-80 parts of carriers. The invention further provides a preparation method and application of the complex microbial inoculant. The prepared complex microbial inoculant has the advantages that the complex microbial inoculant contains enough effective viable bacteria and can take effect stably and efficiently; by the complex microbial inoculant, the soil organic matter and the content of nutritional elements such as nitrogen, phosphorus and potassium can be increased remarkably, nutrient conversion rate and the number of beneficial microorganisms in products can be also increased, soil remediation can be promoted, soil fertility can be improved, and consumption of chemical fertilizer can be reduced; disease resistance of crops can be enhanced, crop quality can be improved, investment can be reduced, and yield and income can be increased.

The invention discloses a cow dung treatment method. The cow dung treatment method comprises the following steps: (1) pre-dehydrating: dehydrating cow dung until the moisture percentage content of thecow dung is 50 percent to 60 percent; (2) pre-fermenting: adding bacillus mycoides and bacillus subtilis into the cow dung and then pre-fermenting; (3) preparing materials: adding an auxiliary material composed of traditional Chinese medicine dreg, lime and germ bran into a fermentation tank and mixing and stirring uniformly; (5) carrying out main fermentation: adding a fermentation bacterium agent composed of cellulose degradation bacteria, xylan degradation bacteria and EM bacteria into the fermentation tank and carrying out the main fermentation; (6) drying: drying the cow dung by utilizing infrared rays until the moisture percentage content of the cow dung is 5 percent to 10 percent. A product obtained by the cow dung treatment method disclosed by the invention can be directly used for cultivating edible mushrooms; the treatment method is simple and convenient, high in efficiency and strong in practicability.

The invention discloses a feed. The feed is characterized by being prepared from by steps: (a) preparation of a fermentation raw material, to be specific, proportionally adding culture solution to feathers to obtain a fermentation raw material; (b) inoculation of a strain capable of degrading the feathers, to be specific, inoculating the obtained fermentation raw material with bacillus paramycoides in a nucleotide sequence shown as SEQ ID NO.1; (c) fermentation, to be specific, fermenting for 20H-80H at 30-45 DEG C under the condition that a PH value is 5-8, so that liquid feed is obtained after fermentation; (d) preparation of solid feed, to be specific, adding a base material into the obtained liquid feed according to a certain proportion, and drying to obtain the solid feed. The feed ishigh in nutrient content, especially high in content of amino acid components and suitable for preparation of preparation of feed or other nutrient products. The feed is mainly prepared from the feathers, so that the feathers are recycled effectively, waste of the feathers is reduced, and the problem of difficulty in treatment of the feathers is solved.

The invention discloses a cellulose degradation complex microbial inoculant and a preparation method thereof. The cellulose degradation complex microbial inoculant is prepared from bacillus mycoides, serratia marcescens, corynebacterium glutamicum, trichoderma reesei and aspergillus fumigatus. The total viable count of the complex microbial inoculant is greater than or equal to 3.75 * 10 < 8 > cfu / mL. The complex microbial inoculant provided by the invention can improve the production efficiency of the organic fertilizer taking the high-cellulose-content organic waste as the fermentation raw material, promote the resource utilization of the high-cellulose-content organic waste such as straw and phytophagous animal manure, and provide a technical basis for the production of the high-quality organic fertilizer and the development of an agricultural waste green treatment mode.

The invention discloses a Bacillus mycoides R2 strain and application thereof in preventing and controlling plant root-knot nematodes. The Bacillus mycoides R2 strain is collected under a number of CCTCC NO: M2015251. The Bacillus mycoides R2 strain is confirmed to be Bacillus mycoides through 16S rRNA analysis and other morphological analysis and named as Bacillus mycoides R2. The Bacillus mycoides R2 strain which is screened out has higher activity in resisting nematodes, so that theories of Bacillus nematicides are enriched. N,N-diethyl-m-toluamide (DEET) and styrene which are generated by the Bacillus mycoides R2 strain has a repelling effect on nematodes, so that a theoretical foundation is laid for development of nematode repellents and the application thereof in nematode prevention and control. Interrelation and action molecule mechanism between pathogenic microorganisms and hosts are clarified, so that a theoretical foundation is laid for application of semiochemicals in the aspects of biological prevention and control of nematodes.

The present invention is intended to provide a reducing agent effective for color development of meat and uses therefor. The present invention provides a reducing agent containing a heme reductase derived from a microorganism belonging to the genus Bacillus. Preferably, crushed bacterial cells of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus natto, Bacillus thuringiensis, or Bacillus mycoides are used.

The invention discloses a bacillus paramycoides fermentation method. The fermentation method is characterized by comprising the following steps: (a) production of a fermentation substrate: sterilizingfeather, and adding a nutrient solution in a certain proportion to obtain the fermentation substrate; (b) inoculation with strains capable of degrading the feather: inoculating the obtained fermentation substrate with a certain proportion of the strains capable of degrading the feather; and (c) fermentation: carrying out fermentation at certain temperature and PH value for 20 H-80 H. The bacillusparamycoides fermentation method which can rapidly and efficiently degrade the feather is provided and can rapidly degrade feather, nutrient ingredients such as amino acid can be obtained after fermentation, the problem that the feather is difficult to treat is solved, and the fermentation process basically causes no environmental pollution.

The invention discloses a composite microbial deodorization microbial agent as well as a preparation method and an application thereof. The composite microbial deodorization microbial agent is prepared from bacillus mycoides, saccharomyces cerevisiae, lactobacillus zeae, bacillus licheniformis, bacillus subtilis, actinomycetes and bacillus aceticus. The preparation method comprises the following steps: S1, separating strains of degrading bacteria from the kitchen waste, and carrying out purification culture; S2, inoculating the culture into a kitchen waste sample, judging the deodorization effect of the culture, and primarily screening out the culture with relatively strong deodorization capability from the culture; S3, re-screening the primarily screened culture to further screen out a plurality of single colony cultures with a good deodorization effect; S4, identifying the plurality of single colony culture strains respectively to determine strain types; S5, performing antagonism identification of a plurality of pure strains; S6, compounding a plurality of pure strains which are not antagonistic to one another; and S7, carrying out deodorization effect measurement on the plurality of pure strains, and selecting out the plurality of strains with good deodorization effect to obtain the composite microbial deodorization microbial agent disclosed by the invention.

The invention discloses a special growth-promoting and pathogenic-bacterium-preventing agent for barley crops. The special growth-promoting and pathogenic-bacterium-preventing agent is prepared from the following raw materials: pseudomonas strain fermentation liquor, bacillus cereus strain fermentation liquor, Bacillus mycoides strain fermentation liquor, decomposed straw, potato residues, ediblemushroom residues, peat, rare earth attapulgite powder, humic acid, potassium fulvate and magnesium sulfate. The invention also provides a preparation method and application thereof. The beneficial effects of the agent are as follows: the agent is is suitable for planting barley crops such as barley and highland barley; the contained active strains can well survive on the surfaces of root systemsof barley crops and in rhizosphere soil; the symbiotic matching performance with barley crops is good; the agent has the effects of antagonizing pathogenic fungi, increasing the effective phosphorus content of soil, fixing nitrogen biologically, secreting plant hormones and the like, can play roles in promoting the growth of barley crops, preventing and treating diseases and improving the adverseenvironmental stress tolerance of the barley crops, and also has the advantages of environmental friendliness, economy, practicability, good sustainability and the like.

A Bacillus mycoides No.4 (CGMCC No.0676) is prepared by separating it from tobacco leaf. It can decrease the irritation of cigarette smoke and the protein and nicotin of cigarette, and improve the smell and taste of cigarette.

The invention discloses a method for large-scale production of a Bacillamide compound and a Bacillamide precursor. The method comprises the steps as follows: actinomycetes are inoculated onto a fermentation culture medium and cultured for 5-10 days; bacillus mycoides are also inoculated onto the fermentation culture medium and subjected to symbiotic culture for 10-20 days to obtain a co-cultured fermentation culture medium, and the Bacillamide compound and the Bacillamide precursor are obtained after separation. The method provided by the invention is low in the requirement on a culture medium, simple to operate, and low in industrial production cost, the fermentation product is simple, high in the content of effective compositions and low in the content of purities, and not only lowers the cost, but also can be in large-scale production to obtain the Bacillamide compound and the Bacillamide precursor, and very high application value and high industrial production feasibility are achieved.

The present invention is intended to provide a reducing agent effective for color development of meat and uses therefor. The present invention provides a reducing agent containing a heme reductase derived from a microorganism belonging to the genus Bacillus. Preferably, crushed bacterial cells of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus natto, Bacillus thuringiensis, or Bacillus mycoides are used.

The invention discloses bacillus mycoides FXY-WH10. The bacillus mycoides has been collected in the China Center for Type Culture Collection (CCTCC), with collection number being CCTCC NO:M 2012263 and collection date being July 1, 2012. The invention also discloses application of the bacillus mycoides for promoting growth of liquidambar formosana. The FXY-WH10 strains have stronger lecithin degradation capabilities. The FXY-WH10 strains are inoculated to the liquidambar formosana seedlings and the results show that the strains can obviously promote growth of the liquidambar formosana seedlings. Therefore, the invention provides excellent strain resources for developing liquidambar formosana phosphorus bacterial fertilizers.

The invention provides a compound microbial agent, and a preparation method and an application thereof to ginger, and belongs to the technical field of microbes. The compound microbial agent at leastcomprises Bacillus lincheniformis LCDD6, Bacillus thuringiensis TA-201, Bacillus thuringiensis TA-202, and Bacillus mycoides TA-203. The compound microbial agent of the invention can provide nutritionfor the growth of ginger. The compound microbial agent of the invention is used to treat ginger, the root activity of ginger can be enhanced, the growth of a ground diameter of ginger can be promoted, and the dry and wet weight of ginger stems and leaves can be increased, so that the plant biomass is improved, the biological yield and quality of ginger are improved, and thereby the compound microbial agent has good practical application values.

The invention discloses a microbial antibacterial agent and a preparation method thereof, and belongs to the technical field of microbes. The microbial antibacterial agent includes a mixed bacterial liquid and assistants, the mixed bacterial liquid is a liquid obtained by mixing Bacillus mycoides Gnyt1 with Pseudomonas sp.ChO19, and the assistants include Tween 80, sorbitol and methylparaben. Theabove two strains and the assistants have synergistic effects, so the microbial antibacterial agent can prevent diseases and promote the growth, and also has good wetting, antiseptic, dispersing and ultraviolet-protecting properties.

The invention belongs to the technical field of biology, and relates to bacillus for biodegradation of uric acid. The bacillus can generate enzyme for catalytic degradation of the uric acid, and the bacillus is a bacillus parabarisiformis YC-02 strain. The invention further relates to a bacillus paramushroom preparation for biodegrading uric acid. The bacillus paramushroom preparation contains one or more of bacterial cells, spores and crude enzyme of the bacillus. Research results show that the bacillus for biodegrading uric acid and the enzyme produced by the bacillus are safe to human bodies, can efficiently biodegrade uric acid, and have important application prospects in the aspects of removing uric acid and treating human body hyperuricemia and corresponding complications.