24 results about "Bacillus mycoides" patented technology

The invention discloses a multifunctional low-temperature corn straw degradation complex microbial inoculant, a preparation method and application, the complex microbial inoculant is compounded by a plurality of microorganisms with straw degradation, potassium dissolving and growth promoting functions, and the complex microbial inoculant comprises the following strains: bacillus subtilis K15, curveolate Prickia L11 and bacillus mycoides L31. The complex microbial inoculant disclosed by the invention realizes synergistic interaction and functional complementation, and through scientific compounding of strains, the straw decomposition rate is remarkably increased, and the decomposition period is obviously shortened; the adaptive capacity and colonization competitiveness of microorganisms in a low-temperature and complex soil environment in winter are enhanced. The complex microbial inoculant disclosed by the invention also has multiple functions of degrading straws, dissolving potassium, producing IAA (Indoleacetic Acid) and the like. The soil potassium which is difficult to utilize can be converted into quick-acting potassium while the straws are quickly decomposed to release nutrients.

The application discloses a kind of multifunctional low-temperature degradation corn stalk composite microbial inoculant, preparation method and application, the composite microbial inoculant is compounded by multiple microorganisms with straw degradation, potassium solution and promoting function, comprising the following strains: bacillus subtilis K15, curvularia geniculata L11 and bacillus mycoides L31.The composite microbial inoculant constructed in the application realizes synergistic effect and functional complementation, through strain scientific compounding, straw decomposition rate is significantly improved, and decomposition period is obviously shortened;Enhance the adaptability and colonization competitiveness of microorganisms in winter low temperature and complex soil environment.The composite microbial inoculant of the application also has multiple functions such as straw degradation, potassium solution and IAA production, etc.At the same time of releasing nutrients by rapid decomposition of straw, it can convert difficult-to-use soil potassium into available potassium.

The invention provides bacillus mycoides Y2 with functions of reducing cadmium content of crops and promoting growth and application of the bacillus mycoides Y2, and relates to the technical field of soil heavy metal pollution bioremediation and agricultural microorganism application. The bacillus mycoides Y2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 14, 2025, and the preservation number is CGMCC 35231. The strain can be applied to cadmium-polluted soil through root application, can reduce the cadmium content of edible parts of crops such as rice, oilseed rape and fast vegetables, and promotes crop growth and rice root surface iron plaque formation. The contaminated soil in Hunan, Henan and Tianjin is verified to be capable of stably colonizing and adapting to different soils and crops. The strain and the matched preparation are green, efficient, high in stability and wide in universality, and provide key technical support for safe utilization of cadmium-polluted cultivated land.

This invention discloses a strain of *Bacillus paramycoides* and its application in selenium oxidation, belonging to the field of agricultural microbiology. A strain of *Bacillus paramycoides* H1 was isolated from the soil of a selenium-nutrition experimental field at Huazhong Agricultural University and deposited at the China Center for Type Culture Collection (CCTCC) on June 27, 2023, with accession number CCTCCNO: M 20231107. Experimental verification showed that this strain can simultaneously oxidize elemental selenium and organic selenium. This invention utilizes this strain to convert organic and elemental selenium into water-soluble selenium that promotes plant absorption, laying a scientific foundation for the preparation of safe and low-cost plant selenium-enriching agents and selenium oxidants. This is of great significance for the research and agricultural cultivation of selenium conversion model strains.

The invention discloses a compound microbial fertilizer suitable for flue-cured tobacco production as well as a preparation method and application thereof. The compound microbial fertilizer is prepared from the following components in percentage by mass: 4-8% of a functional microbial agent, 12-18% of a first modifier, 1-2% of a second modifier, 30-70% of an inorganic material and the balance of pure water, wherein the first modifier is a polyglutamic acid stock solution in which the mass percent of polyglutamic acid is greater than or equal to 3.5%, and the second modifier is a gamma-aminobutyric acid stock solution in which the mass percent of gamma-aminobutyric acid is greater than or equal to 50%; the functional microbial agent is prepared by compounding a bacterial strain XNSD01 and a bacterial strain XNSD02, the bacterial strain XNSD01 is bacillus siamensis with the preservation number of CCTCC (China Center for Type Culture Collection) NO.M 20252934, and the bacterial strain XNSD02 is bacillus mycoides with the preservation number of CCTCC NO.M 20252935. And unification of disease resistance, growth promotion and successive cropping obstacle alleviation is realized.

The present application relates to the field of bio-agriculture, and particularly relates to a bio-agricultural compound microbial fertilizer and a preparation method thereof, which comprises, by weight, 2-8 parts of functional microbial flora, 20-48 parts of organic carrier, 5-20 parts of nutrient component, 0.5-4 parts of microbial body protective agent, and the balance of water; the functional microbial flora is composed of Pseudomonas fluorescens, Trichoderma harzianum, Saccharomyces cerevisiae and Bacillus mycoides; and the organic carrier is composed of a peanut shell-sugarcane residue mixture, modified diatomite and humic acid. The present application forms a complete compound microbial fertilizer technical solution through scientific compounding of multiple strains, directional modification of the organic carrier, balanced optimization of the nutrient component and low-temperature preparation process synergy, and has multiple outstanding beneficial effects.

The present application relates to the technical field of bio-organic fertilizer preparation, and particularly relates to a bio-organic fertilizer prepared by microbial fermentation and a preparation method thereof, which is prepared by fermentation of raw materials and compound microbial strains, wherein the raw materials include livestock and poultry manure, crop straw, industrial organic waste residue and a humification agent, and the compound microbial strains are a ternary combination of bacillus subtilis, bacillus mycoides and saccharomyces cerevisiae, or a quaternary combination of bacillus subtilis, bacillus mycoides, saccharomyces cerevisiae and bacillus mucilaginosus. The present application adopts a multi-element compound strain combination and optimizes the ratio, and the synergistic effect of each strain is significant, which greatly improves the organic matter degradation efficiency and effective nutrient activation capacity, and compared with single strain, the microbial activity is stronger, and the nutrient supply capacity of the fertilizer is better.

The invention discloses a macroelement water-soluble fertilizer and production equipment thereof, and relates to the technical field of water-soluble fertilizer production, and the macroelement water-soluble fertilizer comprises, by weight, 25-30 parts of a nitrogen source, 25-30 parts of a phosphorus source, 20-25 parts of a potassium source, 15-20 parts of an anti-hardening agent, 8-15 parts of a soil improvement agent, 0.2-0.5 part of a microbial agent, and 1.2-1.5 parts of trace elements. Wherein the anti-hardening agent can stimulate plant root systems to secrete polysaccharide viscose substances, and soil particles are cemented to form a stable aggregate structure; meanwhile, alginate oligosaccharide in the anti-hardening agent can be matched with bacillus mucilaginosus to promote the bacillus mucilaginosus to secrete polysaccharide and organic acid, so that the stability of soil aggregates can be enhanced, and soil pores are improved; the polyglutamic acid can adsorb calcium and magnesium ions in the soil and reduce insoluble salt formed by the ions, phosphate radicals and sulfate radicals, so that soil hardening is reduced.

This invention provides a strain of Bacillus mycoides Y2 with functions of reducing cadmium content in crops and promoting growth, and its applications, relating to the fields of soil heavy metal pollution bioremediation and agricultural microbial application technology. Bacillus mycoides Y2 was deposited on July 14, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC35231. This strain can be applied to cadmium-contaminated soil via root application, reducing cadmium content in the edible parts of crops such as rice, rapeseed, and kale, and promoting crop growth and the formation of an iron film on the root surface of rice. Verification with contaminated soils in Hunan, Henan, and Tianjin has shown that it can stably colonize and adapt to different soils and crops. This strain and its accompanying formulation are green, efficient, highly stable, and widely applicable, providing key technical support for the safe utilization of cadmium-contaminated arable land.

The application discloses a bacillus mycoides BMS-39 and application thereof, and belongs to the technical field of microorganisms and agricultural biological control. Fulvia fulva The strain BMS-39 has a very strong inhibiting effect, the inhibiting rate reaches 92.3% by means of a flat-plate confrontation method, the inhibiting rate of 10% fermentation filtrate reaches 87.6%, the potting prevention effect reaches 87.01% in 14 days, and the field prevention effect reaches 87.81%. Even when the concentration of the bacterial liquid is reduced to 10 6 CFU / mL, the prevention effect of the BMS-39 can still reach 81.46%, which means that the fermentation cost and application cost of the application can be greatly reduced under the premise of meeting the same or even better prevention effect, and the application has significant market competitiveness.

The invention discloses a construction method of a novel composite biocontrol flora for preventing and treating cotton verticillium wilt, and relates to the technical field of biological prevention and treatment of agricultural plant diseases. The bacillus polymyxa and the bacillus cereus with the optimal antagonistic activity are respectively screened out through plate confrontation and buckling tests, and the bacillus pseudomycoides is obtained through screening by taking the growth promoting capability of dissolving phosphorus and producing indoleacetic acid as indexes. Through pairwise cross culture verification, the three strains are completely compatible and have no mutual inhibition effect. The method comprises the following steps: respectively and independently activating the three strains, carrying out enlarged culture, preparing single bacterial liquid with the OD600 value of 1.0, and uniformly mixing according to a volume ratio, so as to obtain the novel composite biocontrol flora for preventing and treating the cotton verticillium wilt. According to the invention, antagonistic bacillus and growth-promoting bacillus with complementary functions are screened, single bacteria are independently cultured and then are compounded according to a ratio of 1: 1: 1 to construct a composite flora, so that the synergistic interaction of efficient prevention and control of cotton verticillium wilt, cotton growth promotion and soil phosphorus solubilization is realized.

The application discloses a preparation method of an extractive for preventing and treating citrus anthracnose, and comprises the following steps: drying garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed, and then crushing the dried garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed; adding the crushed garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed into boiling water and stirring until uniform; continuing to cook; freezing; thawing after the freezing is completed; obtaining a freeze-thawed material; adding sodium dihydrogen phosphate into the freeze-thawed material; placing the freeze-thawed material in a fermentation tank; inoculating bacillus subtilis and bacillus mycoides for aerobic fermentation; inoculating bacillus licheniformis, bacillus laterosporus and bacillus thuringiensis for aerobic fermentation; completing the aerobic fermentation; carrying out anaerobic fermentation on the fermented material; stirring the fermented material and pure water until uniform; filtering after standing; taking filter residue and precipitate as extractive A; adjusting the PH value of filtrate to obtain extractive B; adding bagasse powder into the extractive A, compacting, packaging and bagging; and sealing and storing the extractive B. The extractive prepared by the application can efficiently prevent and treat citrus anthracnose, and has the characteristics of low production cost and no pollution to citrus planting environment.

The invention relates to the technical field of planting, in particular to a fungicide and fertilizer suitable for planting landscape plants. The microbial inoculum disclosed by the invention is prepared from the following components in parts by mass: 5 to 10 parts of bacillus coagulans microbial inoculum, 4 to 8 parts of bacillus laterosporus microbial inoculum, 10 to 18 parts of bacillus mucilaginosus microbial inoculum, 6 to 10 parts of bacillus velezensis microbial inoculum, 2 to 6 parts of trichoderma viride, 6 to 10 parts of bacillus amyloliquefaciens microbial inoculum and 2 to 6 parts of paecilomyces lilacinus. Through compatibility of various microbial agents, the soil structure can be improved, and the soil fertility can be improved. The microbial agent disclosed by the invention is prepared into a fertilizer, and the obtained fertilizer also has the effects of regulating the soil microhabitat, improving the soil structure and improving the soil fertility. The growth of garden plant roots can be promoted, and the growth performance of garden plants is improved. And a basis is provided for planting of landscape plants.

The application relates to the technical field of biological fertilizer, and particularly discloses a compound microbial fertilizer and a preparation method thereof. The Klebsiella pneumoniae, Bacillus megaterium, Bacillus mycoides and Bacillus subtilis are compounded, the Klebsiella pneumoniae converts nitrogen in the air into nitrogen available for plants, the Bacillus megaterium decomposes insoluble phosphorus in soil into effective phosphorus, the Bacillus mycoides decomposes potassium silicate minerals in soil to release effective potassium, the Bacillus subtilis produces biological substances to stimulate root growth and secretes antibacterial substances to inhibit pathogenic bacteria in roots. The modified biochar carrier is used to provide stable living space for the compound microbial fertilizer, so that the compound microbial fertilizer is sustainable, efficient and green.

The application discloses a saline-alkali soil conditioner and a preparation method thereof, and belongs to the technical field of soil improvement. The soil conditioner is prepared from the following raw materials in parts by weight: modified phosphogypsum 100-200 parts, microbial agent 5-10 parts, and activator 10-20 parts; the modified phosphogypsum is prepared from phosphogypsum and modified coal gangue after compounding and rare earth modification; the microbial agent is prepared from Bacillus subtilis, Streptomyces cellulans and Bacillus mycoides. The chemical-physical-biological improvement technology is combined, the physical and chemical properties of the saline-alkali soil are effectively improved, the soil biological activity is increased, the growth of crops is promoted, the yield and disease resistance of crops are improved, and the resource utilization rate of phosphogypsum is improved.

The present application belongs to the technical field of drought resistance of Mesona chinensis Benth, and particularly relates to a microbial agent for improving drought resistance of Mesona chinensis Benth. The microbial agent for improving drought resistance of Mesona chinensis Benth is composed of Bacillus circulans bacterial liquid and Bacillus mycoides bacterial liquid, and the volume ratio of the Bacillus circulans bacterial liquid to the Bacillus mycoides bacterial liquid is 1:3-5. The microbial agent of the present application is composed of the Bacillus circulans bacterial liquid and the Bacillus mycoides bacterial liquid in a volume ratio of 1:3-5, and the Bacillus circulans bacterial liquid and the Bacillus mycoides bacterial liquid are respectively obtained by culturing corresponding strains in a liquid culture medium. During the planting of Mesona chinensis Benth, the microbial agent of the present application can significantly delay the damage to the Mesona chinensis Benth plants under drought stress, and is helpful to improve the drought resistance of the Mesona chinensis Benth, which is of great significance to the development of the Mesona chinensis Benth industry.

The invention belongs to the technical field of agricultural biology, and particularly discloses a functional seedling culture substrate for preventing and treating root-knot nematode and application of the functional seedling culture substrate. The functional seedling raising substrate is composed of a solid seedling raising substrate and a compound microbial agent uniformly dispersed in the solid seedling raising substrate. The compound microbial agent is prepared by carrying out mixed fermentation on bacillus velezensis RKN1111, bacillus laterosporus CDD-01 and paenibacillus mucilaginosus HX-02. The invention further discloses a preparation method of the compound microbial agent. When the functional seedling culture substrate is used for seedling culture, the colonization rate of the compound microbial agent in seedling rhizosphere is greatly improved, the inside and the outside of root lumps of cultured germchit are rich in active biocontrol bacteria during transplanting, the germchit can continuously play a high-efficiency and stable prevention and control role after transplanting, and the growth of the germchit is remarkably promoted. According to the method, the prevention and control opportunity of the root-knot nematode is greatly advanced from a field period to a controllable seedling raising period, active immunization is achieved, and the technical problems that colonization is difficult and the effect is unstable when a biocontrol microbial inoculum is applied to traditional root irrigation or fields are effectively solved.

The present application provides a bacillus mycoides and its application in synthesizing manganese oxide, and the bacillus mycoides (Bacillus mycoides) CP203 with a preservation number of CGMCC No. 24208 is provided. Bacillus mycoides The present application further provides the application of the aforementioned bacillus mycoides in biosynthesis of manganese oxide, which can oxidize divalent manganese ions in a solution into manganese oxide without contact, the synthesized manganese oxide product does not contain organisms such as bacteria and spores, and the divalent manganese ions and other harmful substances in raw materials will not adversely affect the growth of microorganisms, which can significantly reduce the purification cost of biosynthesis of manganese oxide, expand the types and range of biologically treated manganese ion wastewater, and therefore the present application has important applications in the fields of biosynthesis of manganese oxide and hazardous waste water treatment.

The invention discloses a method for preparing vegetation planting soil by utilizing industrial solid waste and microorganisms and application of the vegetation planting soil. The method comprises the following steps: mixing 80-90% of high titanium slag, 7-15% of sandy soil or phosphogypsum and 2-5% of titanium gypsum into a vegetation matrix according to specific particle size grading; inoculating a composite strain consisting of bacillus mucilaginosus and azotobacter chroococcum, fermenting at 30 DEG C for 12-18 days, and dynamically monitoring the contents of alkali-hydrolyzable nitrogen, available phosphorus and potassium and biologically extractable titanium; then, alfalfa is planted, arbuscular mycorrhizal fungi and rhizobium suspensions with the OD600 being 0.8-1.0 are regularly irrigated, and curing is conducted for 12-18 days; and irrigating and maintaining for 12-18 days by using chlorella propagation liquid every 4-6 days, and finally reserving the whole plant or harvesting to obtain the plant growing planting soil. According to the method, industrial solid waste is efficiently converted into a functional planting medium through the multi-stage biological-mineral synergistic effect, solid waste recycling and soil function reconstruction are achieved, and the method has the environment restoration and ecological reconstruction value.

The present invention addresses the problem of providing a nicotinamide mononucleotide (NMN) transport protein that can efficiently recover NMN that has been synthesized using a fermentative production method from an extracellular environment.　Provided is a PnuC mutant that has a mutation (an 81A mutation) that replaces the amino acid at the position that corresponds to the 81st position in the amino acid sequence of SEQ ID NO:1 with alanine in PnuC derived from Bacillus mycoides or an ortholog protein from another species of genus Bacillus or Paenibacillus (including different strains of the same species).

This invention relates to the field of ecological cultivation technology of Chinese medicinal materials, specifically to a comprehensive prevention and control method for iron deficiency in *Polygonatum sibiricum* under forest cover. This invention addresses the symptoms of iron deficiency in *Polygonatum sibiricum* under forest cover by adjusting soil pH, providing effective iron supply, and combining a compound microbial agent of *Bacillus subtilis*, *Bacillus pumilus*, *Bacillus tekirae*, *Bacillus mycoides*, and *Bacillus amyloliquefaciens* with *Pseudomonas fluorescens* to release iron carriers. This fundamentally solves the problems of chlorosis and yellowish-white discoloration in young leaves, oily yellowish-brown spots on leaves, wilting of young shoots, elongated internodes, and easy lodging. Using *Polygonatum sibiricum* as the material, this invention proposes a three-step comprehensive approach to eliminate deficiency symptoms caused by iron deficiency during its ecological cultivation in forests. This allows the chloroplast thylakoid membrane and chlorophyll synthesis in *Polygonatum sibiricum* plants under forest cover to recover, photosynthesis to return to normal, and chlorophyll fluorescence kinetic parameters to a healthy state.

The invention discloses a planting method for simultaneously improving saline-alkali soil health and crop yield. The method comprises the following steps: mixing bacillus subtilis, bacillus amyloliquefaciens and paenibacillus mucilaginosus to prepare a compound microbial agent; mixing the compound microbial agent with a chemical fertilizer to prepare a base fertilizer; in the crop sowing period, the base fertilizer is applied to the lower side of crop seeds; a microbial activator is conveyed; after the crops are mature, the leguminous crops and the cruciferous crops are subjected to multiple cropping; harvesting overground parts of the cruciferous crops as silage; crushing leguminous crops to cover the earth surface in a frost period; annual circulation is carried out in this way. Through integration of three technologies of microbial agent stress resistance, drip irrigation activation and green manure graded management, in-season improvement and high yield of moderate saline-alkali soil (the salt content gt is 0.6%) are achieved, soil available nutrients are improved, soil fertility is improved, spring wheat yield and grain quality are improved, and double improvement of soil health and crop yield is achieved.

The invention belongs to the technical field of pachira macrocarpa planting, and particularly relates to a water and fertilizer integrated planting method for pachira macrocarpa under a eucalyptus forest. S2, planting pachira macrocarpa; s3, constructing a water and fertilizer integrated system; s4, trickle irrigation of water and fertilizer: trickle irrigation of special fertilizer for pachira macrocarpa from field planting to outplanting; the special fertilizer for pachira macrocarpa is prepared by mixing a microbial fertilizer and a water-soluble fertilizer according to a mass ratio of 1: 8, wherein the microbiological bacterial fertilizer is prepared from bacillus safensis fermentation liquor and bacillus mycoides fermentation liquor; s5, performing field management. By the adoption of the planting method, the pachira macrocarpa is high in survival rate and good in growth situation, the average height, the average stem diameter and the average leaf number of plants are obviously better than those of plants in a traditional planting mode, leaves are dark green and glossy, stems are robust, root systems are developed, the outplanting time can be shortened, the high-quality outplanting standard can be met in advance, and the economic benefit of planting is further improved.

The invention belongs to the technical field of microorganisms, and relates to bacillus paramushroom for tobacco, a culture method and application in tobacco fermentation, the bacillus paramushroom is characterized in that the preservation number of a bacillus paramushroom strain S-65 is CGMCC No.35209, and the bacillus paramushroom strain S-65 is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 11, 2025; the bacillus parabarium strain S-65 disclosed by the invention can be used as a fermentation strain to act on redried tobacco leaves to play a role; according to the bacillus parabarium disclosed by the invention, the contents of 9-hydroxy-4, 7-megastigmatriene-3-ketone, dihydroactinidiolide, farnesyl acetone, geranyl acetone and 4, 7, 9-megastigmatriene-3-ketone in redried tobacco leaves can be increased, farnesyl alcohol, damascenone, (+)-limonene and capraldehyde are newly generated substances after fermentation, and the content of the 9-hydroxy-4, 7-megastigmatriene-3-ketone, the content of the dihydroactinidiolide, the farnesyl acetone, the geranyl acetone and the 4, 7, 9-megastigmatriene-3-ketone in the redried tobacco leaves can be increased. The smoke state is improved while the aroma substances are increased, the aroma texture and sweetness are increased, and the aftertaste comfort is improved.