33 results about "Centrifugal microfluidics" patented technology

The invention discloses a rapid detection method for fusarium wilt in corn based on an RPA-CRISPR / Cas12b technology, and belongs to the technical field of nucleic acid detection. The invention provides a specific reagent combination, a kit and a detection method in order to solve the problems of high amplicon pollution risk, complicated operation and the like in the existing technologies such as RPA (recombinase polymerase amplification). The reagent combination comprises an RPA (recombinase polymerase amplification) primer pair with sequences as shown in SEQ ID NO.1 and SEQ ID NO.2, and sgRNA (single guide ribonucleic acid) with a sequence as shown in SEQ ID NO.3. The method comprises the following steps: carrying out RPA amplification by taking nucleic acid of a sample to be detected as a template, carrying out mixed incubation on an amplification product, sgRNA, Cas12b protein and fluorescent reporter molecules, and judging a result by detecting a fluorescent signal. The optimized RPA and CRISPR systems are integrated on the centrifugal micro-fluidic chip, so that the full-flow closed tube and automatic detection is realized, the detection can be completed within 30 minutes, the detection sensitivity reaches 1E2 copies / T, the risk of aerosol pollution is greatly reduced, and the centrifugal micro-fluidic chip is suitable for port quarantine and field on-site rapid detection.

The invention discloses a detection kit and method for detecting allergic inflammatory factors based on a centrifugal micro-fluidic chip, and relates to the field of kits, the detection kit comprises the centrifugal micro-fluidic chip and a preset reagent; the centrifugal micro-fluidic chip comprises a chip base body, a sample adding cavity, a liquid level sensor, a liquid level sensor, a liquid level sensor, a liquid level sensor and a liquid level sensor, the nucleic acid extraction and purification cavity comprises a plurality of amplification cavities; a reagent storage chamber; a waste liquid chamber; and a capillary valve. The functions of nucleic acid extraction, variable temperature amplification and multi-target synchronous detection are integrated through the centrifugal micro-fluidic chip, full-closed operation is achieved by means of centrifugal force or other driving force such as air pressure and electroosmotic flow, and the pollution risk caused by manual step-by-step operation is thoroughly eliminated; a multi-wavelength distinguishable fluorescent probe and a grouping preset primer combination are adopted to synchronously complete parallel detection of the allergic inflammatory factor mRNA and the drug accompanying diagnosis target, cross interference is avoided, and large instruments and equipment do not need to be relied on.

The invention provides a centrifugal micro-fluidic chip for a method for rapidly enriching nucleic acid by microbial biochar, and aims at a solid-phase carrier for adsorbing nucleic acid, and the centrifugal micro-fluidic chip capable of completing the whole process of nucleic acid elution, amplification and detection is designed. A quantitative cavity, an elution cavity and a detection area are arranged in the chip, the elution cavity is used for eluting nucleic acid to be detected from biochar, and the quantitative cavity is used for temporarily storing added eluent and quantifying the added eluent to accurately complete the elution process; the structure of the quantitative cavity, the structure of the elution cavity and the structure of the fluid channel are improved, and steps are additionally arranged in the fluid channel, so that a to-be-detected sample smoothly enters a detection area for detection through the capillary tube under the action of surface tension, and the capillary tube can be prevented from being blocked by solid-phase carriers used in pretreatment such as charcoal and magnetic beads; the accuracy of a detection result is ensured; the chip is simple in structure, low in cost, convenient to assemble and easy to layer and sterilize, can simultaneously realize high-throughput detection of more samples and more detection items, and improves the detection efficiency.

A centrifugal microfluidic technique for heat treating emulsion-divided independent reaction volumes (IRVs) within a centrifugal microfluidic chip, and displacing the emulsion into a monolayer presentation chamber (pc) for imaging. A deep treatment chamber (tc) is provided for the heat treatment, a nozzle having a hydrodynamic radius for forming the IRVs is provided for injecting a sample for the IRVs into the tc filled with a dense immiscible medium. The tc is adjacent a heat controlled element for collectively heat treating the IRVs within the tc, where the IRVs form a 3d packing arrangement. The tc is coupled to a presentation chamber (pc) by an opening through which the IRVs can be selectively displaced without collapsing. The pc is adjacent a window transparent to a wavelength for inspecting the pc.

The invention discloses a centrifugal micro-fluidic all-in-one machine structure, and relates to the field of chemical analysis equipment, the centrifugal micro-fluidic all-in-one machine structure comprises a control main body, one end of the control main body is provided with a centrifugal micro-fluidic structure, the centrifugal micro-fluidic structure comprises a mounting frame, and the mounting frame is connected with the control main body; the installation frame is arranged to be of a concentric circle structure composed of a large circular frame and a small circular frame, and a plurality of partition plates are arranged between the large circular frame and the small circular frame. The inner supporting plate and the outer supporting plate rotate independently, different rotating speeds are achieved in cooperation with the speed change gear set, the first detection chip and the second detection chip operate separately, the step breaks through the limitation of the single rotating speed, the multi-link centrifugal requirement is met, and the detection efficiency and flexibility are improved.

The invention discloses an external gyromagnetic drive centrifugal micro-fluidic chip and device suitable for distributed deployment. The centrifugal micro-fluidic chip comprises a chip body (3) and a magnetic driving structure arranged on the chip body, wherein the magnetic driving structure is arranged around the rotation center of the centrifugal micro-fluidic chip. The centrifugal micro-fluidic chip device comprises the centrifugal micro-fluidic chip and a driving device module, wherein the driving device module comprises a stator disc (1), a rotating magnetic field driving control circuit (6) and a driving part shell (5); a plurality of coils (11) are arranged on the stator disc, the rotating magnetic field driving control circuit can control all the coils to generate a rotating magnetic field, and the centrifugal micro-fluidic chip can rotate under the action of the rotating magnetic field. The centrifugal micro-fluidic chip and the centrifugal micro-fluidic device are realized by adopting a simple structure and do not need to occupy a lot of placement space.

The invention discloses a rapid detection method for Xanthomonas oryzae pv. Oryzae on the basis of RPA-CRISPR / Cas12b. The reagent combination comprises a specific RPA primer pair and sgRNA, and the sequences of the specific RPA primer pair and the sgRNA are respectively shown as SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The detection method comprises the following steps: taking nucleic acid of a sample to be detected as a template, and performing RPA isothermal amplification by utilizing the primer pair; the amplification product is mixed with sgRNA, Cas12b protein and fluorescent reporter molecules for incubation, and the trans-cleavage activity of Cas12b is activated; a result is judged by detecting a fluorescence signal. According to the invention, the optimized RPA and CRISPR systems are integrated on the centrifugal micro-fluidic chip, so that the full-flow closed tube and automatic detection is realized, the detection can be completed within 30 minutes, the sensitivity reaches 0.156 pg / reaction, no non-specific reaction is caused to five related bacteria such as Xa and Xap, the sensitivity to XOO3 subtype is high, but the kit is not suitable for XOO2 subtype, and the kit has the advantages of rapidness, sensitivity, specificity and low equipment dependence degree, and can be used for detecting the related bacteria of XOO3 subtype and XOO2 subtype. The method is suitable for non-diagnostic detection of field environments, port environments and the like.

This application discloses a reaction control device for a centrifugal microfluidic chip, relating to the field of in vitro diagnostic technology. The device includes: a chip holder for mounting the centrifugal microfluidic chip, which can accommodate a sample to be tested; a drive module connected to the chip holder and capable of rotating the chip holder; a heating and temperature control module connected to or located on one side of the chip holder, capable of controlling the temperature of the centrifugal microfluidic chip on the chip holder; a fluorescence detection module located below the chip holder, used to detect the reaction of the sample to be tested, wherein the chip holder has fluorescence detection holes adapted to the fluorescence detection module; and a magnetic bead manipulation module adapted to the heating and temperature control module, used to assist in the processing of the sample to be tested. This application achieves integrated operation of the centrifugal microfluidic chip from sample processing and reaction control to result detection through the organic integration and coordinated control of multiple modules.

The invention relates to the technical field of exosome enrichment, in particular to a centrifugal micro-fluidic chip and method for automatically and rapidly enriching and detecting exosomes. Comprising a sample chamber, a sample quantification chamber, a waste sample chamber, a reagent chamber, an efficient mixing chamber, a high-abundance protein removal chamber, an exosome sorting chamber, a carrier generation chamber, an exosome quantification storage chamber, an exosome total storage chamber, a gas generation chamber and a reagent storage chamber, the liquid conveying channel and the gas conveying channel are used for connecting the chambers and controlling the fluid to flow directionally; the rotating speed, direction and time of the chip are controlled through programming of a centrifugal machine, automatic enrichment of exosomes is achieved, and the exosome sorting cavity is configured to capture the exosomes through a bubble carrier and E-Am functional molecules. The exosome sorting device does not depend on a complex liquid pumping system and a mechanical arm, exosome sorting is achieved by programming the centrifugal sample feeding machine, the exosome is not damaged, the sorting speed is high, and the cost is low.

The invention relates to the field of pesticide residue detection, in particular to an application method and system of a pesticide residue detection micro-fluidic chip. The chip design of pesticide residue detection equipment is completed by starting with a micro-fluidic technology and combining with a pesticide residue enzyme inhibition detection method, and the application method of the pesticide residue detection micro-fluidic chip is based on a centrifugal micro-fluidic technology and an enzyme inhibition method detection principle. The design of a disposable pesticide residue detection chip and the design of a reagent freeze-drying scheme in the chip are completed, a high-light-transmittance PMMA plate is selected as a chip material according to detection optical characteristics, the thickness of the high-light-transmittance PMMA plate is 2.5 mm, the thickness of the high-light-transmittance PMMA plate is 0.5 mm, and a precise milling and viscose bonding method is selected according to the equipment development stage characteristics to manufacture the chip.

The invention discloses a rapid detection method for three pathogenic bacteria of rice based on an RPA-CRISPR / Cas12b technology, and belongs to the technical field of nucleic acid detection. The method provides a specific RPA (recombinase polymerase amplification) primer pair and an sgRNA (single guide ribonucleic acid) sequence for three quarantine pests, namely BG (rice blight), XOC (rice bacterial leaf streak) and XOO (rice bacterial leaf blight). Recombinase polymerase amplification (RPA) and CRISPR / Cas12b detection technologies are combined and are integrated on a centrifugal micro-fluidic chip, so that high-sensitivity and high-specificity detection on multi-target nucleic acid is realized, integrated detection of'sample input-result output 'can be completed within 30 minutes, uncovering operation is effectively avoided, the risk of aerosol pollution is greatly reduced, and the method is suitable for industrial production. The method is suitable for port quarantine and field on-site rapid detection, and an efficient technical means is provided for early diagnosis and prevention and control of rice diseases.

This invention belongs to the field of microfluidics technology, specifically relating to a multi-channel centrifugal microfluidic reactor with controllable diameter and multi-layer coating. It includes a body, a centrifugal microfluidic unit, and a liquid accumulation unit. The multi-layer coated droplet initial formation unit within the centrifugal microfluidic unit includes a driven shaft. The output end of a rotation drive unit is connected to the driven shaft, driving it to rotate. A cylindrical liquid injection chamber is centrally located inside the driven shaft, surrounded by multiple annular liquid injection chambers. Microfluidic connectors, the same number as the vertical channels, are connected below the driven shaft. These connectors communicate with both horizontal and vertical channels, and each connector contains a liquid confluence point. A needle is connected below each connector. The liquid accumulation unit includes a liquid accumulation cup. This invention features high throughput, low coefficient of variation, precise controllable liquid capsule size, and the ability to achieve multi-layer coating.

Disclosed are packaging trays, tray assemblies, supporting packs and other devices for use in centrifugal microfluidics. A tray includes a stepped support for stacking a plurality of disc assemblies. The tray also includes retaining members for preventing undesired movement of a disc assembly when it is placed in the tray.

This invention discloses an externally rotating magnetically driven centrifugal microfluidic chip and device suitable for distributed deployment. The centrifugal microfluidic chip of this invention includes a chip body (3) and a magnetic drive structure disposed on the chip body, the magnetic drive structure being arranged around the rotation center of the centrifugal microfluidic chip. The centrifugal microfluidic chip device includes the centrifugal microfluidic chip and a drive device module. The drive device module includes a stator disk (1), a rotating magnetic field drive control circuit (6), and a drive housing (5). Several coils (11) are disposed on the stator disk, and the rotating magnetic field drive control circuit can control all coils to generate a rotating magnetic field, allowing the centrifugal microfluidic chip to rotate under the action of the rotating magnetic field. The centrifugal microfluidic chip and device of this invention are implemented with a simple structure, requiring no large placement space.

The application provides a chip and a full-automatic matching detection device for an interferon-gamma release assay, and belongs to the technical field of in-vitro diagnosis. The device comprises three linear stations of a warehouse position, a heating incubation station and a reaction detection station, the chip is a centrifugal microfluidic detection chip, and the chip is composed of a whole blood incubation and extraction chip and a reaction detection chip. The whole blood incubation and extraction chip is preloaded with liquid reagents suitable for specific detection items, and cooperates with the heating incubation station to complete sample addition and incubation of the sample to be detected. The reaction detection chip is preloaded with general reagents for IGRA detection, mainly cooperates with the reaction detection station to realize capture, feedback and output of the detection signal. The application realizes separation of specific reagents and general reagents for detection items through the double-chip structure, can flexibly replace the incubation chip according to different detection requirements, significantly reduces the cost while ensuring the detection efficiency, and improves the applicability and flexibility of IGRA detection.

The invention discloses a full-automatic nucleic acid extraction, amplification and detection system based on centrifugal microfluidics, and relates to the technical field of microfluidics. Comprising a chip, a magnet switching device and a magnetic attraction fixing device, the chip comprises a chip body and a bottom plate, the chip body and the bottom plate are positioned, stacked and hermetically connected, the chip completes sample splitting, nucleic acid extraction, purification, amplification and detection of molecular diagnosis, and the magnetic attraction fixing device fixes the chip on a supporting disc of the detection device. The magnet switching device is used for generating a controllable magnetic field and driving the first magnetic beads to move in the chip, so as to realize extraction and separation of nucleic acid. According to the invention, a centrifugal microfluidic technology is utilized to highly integrate key processes such as sample lysis, nucleic acid extraction, purification, amplification and detection required by molecular diagnosis and realize automation, so that a rapid, accurate and low-cost detection result capable of effectively preventing cross contamination is provided.

The invention discloses a centrifugal micro-fluidic chip for liquid drop digital nucleic acid amplification detection, the centrifugal micro-fluidic chip comprises a disc body and an adaptive pressure-sensitive adhesive film layer, and the disc body comprises a sample loading area arranged at the periphery of a centrifugal shaft hole; the front end of the extraction channel is connected to the sample loading area, and the rear end is connected with the switching valve; the magnetic bead nucleic acid extraction area is distributed on the outer edge of the extraction channel and is provided with an embedded magnet array; the collecting cavity is connected to the output end of the switching valve; a quantitative distribution channel; a gelatin-based thermosensitive valve; and the plurality of water-in-oil droplet generation and reaction cavities are annularly and uniformly distributed at the edge of the disc body and are communicated with the collection cavity. The chip realizes full-process automation of nucleic acid extraction, droplet generation and amplification detection through cooperation of centrifugal force and a functional structure, is simple and convenient to operate, high in sensitivity and accurate in result, and is suitable for the fields of gene diagnosis, pathogen detection and the like.

A centrifugal microfluidic platform is combined with a stationary liquid pumping system which pumps liquids into microfluidic chips by dripping through a stationary dispensing nozzle without any physical contact or coupling between the nozzles and the microfluidic chips.

The application discloses a centrifugal microfluidic chip for allergen detection, comprising a chip main body and a plurality of microstructures, wherein the chip main body has a rotation center, and the microstructures comprise: a waste chamber located at the farthest end of the rotation center; a reaction detection chamber provided with a carrier, the carrier being coated with anti-human IgE antibodies; a circular truncated cone-shaped channel, the narrow end of which is connected with the other end of the reaction detection chamber, and the wide end of which is connected with a main channel; a serum separation structure comprising a blood cell chamber and a serum chamber, the serum chamber being connected with the main channel through a siphon valve; a first buffer solution chamber; an allergen chamber; a second buffer solution chamber; an enzyme storage chamber; a third buffer solution chamber; a bottom liquid chamber; and a termination liquid chamber. The application avoids contamination and evaporation of reagents, does not need additional driving equipment, is conducive to integration and miniaturization of the equipment, saves consumption of sample whole blood and reagents, reduces cost and time, and is simple to operate; the application needs small amounts of sample and reagents, has low cost, short detection time, and high detection sensitivity.

The invention belongs to the field of virus detection, and particularly relates to a method for detecting geminivirus in tomato plants and bemisia tabaci based on an aMIRA-CRISPR / Cas14a system. The invention discloses a method for detecting geminivirus in a tomato plant and bemisia tabaci based on an aMIRA-CRISPR / Cas14a system. The method comprises the following steps: 1) performing DNA extraction on a tomato plant sample and the bemisia tabaci; 2) specifically amplifying target genes of geminivirus in tomato plants and bemisia tabaci through an asymmetric multi-enzyme constant-temperature rapid amplification technology to obtain an aMIRA amplification product; and 3) a CRISPR / Cas14a system is adopted, and specific sgRNA is designed to recognize single-stranded DNA in an amplification product, so that non-specific cleavage activity of Cas14a is activated, a fluorescent probe is cleaved, release of a fluorescent signal and interpretation of a detection result are realized, and accurate detection of a target virus is realized. The invention develops an AI enhanced MaC14a platform, based on a portable centrifugal microfluidic system, an aMIRA amplification technology and a CRISPR / Cas14a recognition mechanism are integrated, and onsite visual rapid and sensitive detection of geminivirus in tomato and bemisia tabaci can be realized.

This invention belongs to the field of biological detection technology and is used to solve the problem that existing technologies cannot identify specific distortion patterns caused by shared resource competition from multi-channel fluorescence data streams. Specifically, it is a method and system for detecting diarrhea virus multiplex centrifugal microfluidic chips, including: acquiring the growth coefficients of multiple detection channels during thermal cycling, recording the event cycle number of events that cause curve morphology abrupt changes; constructing a feature vector set containing event temporal features, spatial features, and correlation features, and calculating a competition fingerprint index; determining whether to enter a resource competition calibration mode based on the matching relationship between the competition fingerprint index of the feature vector set and a preset range; this invention maps curve morphology abrupt changes of different causes to differentiated quantitative index ranges; and effectively separates distortions caused by resource competition from distortions caused by global system anomalies, local physical anomalies, and temperature disturbances.

This invention discloses a non-clogging, multi-stage microplastic particle grading and sorting device based on centrifugal microfluidics, belonging to the field of microplastic particle analysis technology in aquatic environments. It includes one or more analysis units arranged around the centrifugal axis of a centrifugal microfluidic chip. Each analysis unit consists of a digestion module and a microplastic particle size sorting and capture module. The microplastic particle size sorting and capture module includes an enhanced liquid chamber, a particle size sorting zone, a squeezing microfluidic channel, a gradient particle size separation zone, and a waste liquid chamber. This invention utilizes the coupling of centrifugal force and Coriolis force, along with an anti-angle array, to classify microplastics of different particle size ranges. The angle between the centrifugal force direction and the anti-angle array is greater than 90°, achieving a non-clogging effect on the array, resulting in a high microplastic particle capture rate and small error. Furthermore, centrifugal force is used to achieve fine sorting of microplastics within different particle size ranges based on size, improving the problem of small particle size sorting range for microplastics on a single chip. This invention also discloses a non-clogging, multi-stage microplastic particle detection method based on centrifugal microfluidics, which can rapidly and accurately sort and detect target microplastics of different particle sizes and compositions, offering significant advantages in rapid on-site detection of microplastics.

The application discloses a centrifugal micro-fluidic chip with precise temperature control, which comprises a passive circuit board, a substrate and a heating device, a first conductive element is arranged between the passive circuit board and the substrate, a plurality of reaction cavities are arranged in the substrate, the heating device is used for providing heat for the temperature rise of liquid in the reaction cavities, a first temperature detecting element is arranged in one of the reaction cavities, the first temperature detecting element can reduce the temperature of the liquid to be detected, the first temperature detecting element is connected with the first conductive element, and the passive circuit board and the substrate are connected with a centrifugal rotating shaft. The application can realize the precise control of the temperature of liquid in the chip, the accuracy is up to ±0.1 DEG C, and the temperature change response is fast. The temperature detecting element is embedded in only one reaction cavity, so that the temperature detecting element embedded in the cavity is prevented from directly contacting with the reaction liquid, cross contamination is avoided, and the accuracy of subsequent biochemical reaction and detection is influenced.

Packaging trays, tray assemblies, support packs, and other devices for use in centrifugal microfluidics are disclosed. The tray includes a stepped support for stacking a plurality of tray assemblies. The tray also includes a retaining member for preventing undesired movement of a tray assembly when the tray assembly is placed in the tray.

The application discloses a centrifugal microfluidic isothermal amplification chip, and relates to the technical field of in-vitro diagnosis. The centrifugal microfluidic isothermal amplification chip is a layered structure with a center expanding towards a periphery. The layered structure comprises, from the center to the periphery, a fixed hole, an isolation layer, a nucleic acid extraction layer and a liquid separation reaction layer. The layers are connected through interlayer channels, and fluid is driven to flow through each functional area in sequence through centrifugal force. At least part of the interlayer channels or each functional area is provided with a phase change material to form a phase change valve. The application has the technical effects of high efficiency, low pollution and easy control.

The application discloses a centrifugal microfluidic-based detection chip and application thereof. The detection chip comprises a flow channel layer arranged in sequence in a vertical direction, wherein a sample chamber, a reagent chamber and a reaction flow channel are arranged on the flow channel layer; the sample chamber is used for loading sample liquid, the reagent chamber is used for loading reagent, and the sample chamber and the reagent chamber are respectively communicated with the reaction flow channel and can respectively send out the sample liquid and the reagent to the reaction flow channel under different rotating speeds; and a substrate layer has a first surface adjacent to the flow channel layer, wherein a capture is fixed on the first surface and forms a strip, the strip at least partially overlaps with the reaction flow channel in the vertical direction to form a detection area, and the capture is used for capturing a target in the sample liquid. The detection chip combines the centrifugal microfluidic technology and the detection technology, coats the capture on the substrate layer, adds the sample and the reagent into the reaction flow channel by controlling the size of the rotating speed, and completes the steps of adding the sample and the reagent and incubating in the detection area, thereby improving the automation degree of detection.

The invention discloses a centrifugal microfluidic nucleic acid detection device and method for two-step nucleic acid amplification and detection. The two-step nucleic acid amplification and detection integrated micro-fluidic chip is designed for two-step nucleic acid amplification and detection experiments, mixing of reaction liquid is achieved based on a centrifugal mode, samples enter different chambers for reaction at different rotating speeds, and the problems of system reaction integration and automation are solved. Efficient amplification and high-specificity recognition of nucleic acid are completed, and the detection sensitivity and accuracy are remarkably improved. Through the structural design, a user is supported to load and replace the centrifugal micro-fluidic chip, the operation is simple, the power consumption is low, and the requirement of field detection can be met.

Various aspects disclosed relate to a centrifugal microfluidic device to perform dynamic solid phase sodium bisulfate conversion. The device includes a reaction assembly. The reaction assembly includes a plurality of individual chambers, each including a bisulfate conversion chamber, an elution chamber, a magnetic manipulation chamber, a waste chamber, and a buffer chamber. The reaction assembly further includes at least one valve configured to selectively establish or prevent fluid communication along a channel between at least two respective individual chambers amongst the plurality of individual chambers. Additionally, the reaction assembly is configured to establish fluidic transport in response to rotation about an axis intersecting a central region of the reaction assembly and perpendicular to a plane on which the reaction assembly is disposed.

According to the centrifugal micro-fluidic chip for nucleic acid detection and the rapid preparation method of the centrifugal micro-fluidic chip, the corrosive liquid is injected into the mold, a proper corrosive liquid formula is screened, and a micro-channel can be prepared on glass at high precision, so that the centrifugal micro-fluidic chip for nucleic acid detection is efficiently prepared at low cost; the chip adopts a sandwich structure consisting of a plastic upper layer, a silica gel inner film and a plastic lower layer, and is quickly assembled through two groups of semicircular acrylic buckles; by optimizing the components of the eluent and designing the structure of the micro-fluidic chip, the automatic elution of the adsorbed nucleic acid in the solid-phase carrier is realized, no redundant liquid transfer step is needed, the risk of aerosol pollution and human errors is reduced, and a rapid and convenient highly-integrated detection platform is constructed.