55 results about "Centrifugal microfluidics" patented technology

Disclosed are a centrifugal micro-fluidic device and an immunosorbent assay method using the same. In particular, a centrifugal micro-fluidic device having a plurality of micro-fluidic structures placed in a disc type platform to simultaneously conduct several immunosorbent assays, as well as an immunosorbent assay method using the same are provided.

The invention discloses a separate detection system and method of rare cells based on a centrifugal micro-fluidic technology. The system comprises a micro-fluidic chip similar to an optical disk, a centrifugal drive module and an optical detection module, wherein the micro-fluidic chip comprises a plurality of groups of micro-channels and micro-cavities arranged in a radiation manner; and the entire chip structure is composed of an elastic micro-column guide rail layer, a deformable film layer, a pipeline/cavity layer, a filtering membrane layer and a liquid waste collecting layer. The method comprises the steps of: firstly, leading a sample solution and immune modified microspheres into a liquid storage cavity through an injection port of the micro-fluidic chip in use, putting the sample solution and the immune modified microspheres on a centrifugal platform of the centrifugal drive module, assembling an elastic micro-column, and rotating at a low speed, so as to achieve fully mixing and reacting of the sample solution and immune modified microsphere liquid in the liquid storage cavity, separating by a high-speed rotary chip, and then dropwise adding a specifically recognized fluorescently-labeled antibody solution in each separate cell collection area, carrying out incubate reaction, adding a buffer solution and centrifuging, and finally identifying and analyzing through the optical detection module.

The application relates to a microfluidic chip and an analysis system. The microfluidic chip comprises a chip matrix, a sample adding chamber, an air outlet, a sample enrichment and pyrolysis chamber,a first waste liquid chamber, a dilution chamber, a capillary valve, a second waste liquid chamber, a sample adding chamber circulation pipeline, a pyrolysis chamber circulation pipeline, an air circulation pipeline, a sample output pipeline, a reagent distribution pipeline and a plurality of PCR amplification chambers; the sample output pipeline is provided with a phase change valve and besidesthe chip matrix is provided with an encapsulation hole communicating with the phase change valve; and positioning areas are arranged in the chip matrix. The microfluidic chip is suitable for centrifugal microfluidic analysis and can realize a molecular diagnostic function of nucleic acid-free purification, the phase change valve can be conveniently placed in the sample output pipeline through theencapsulation hole to control entering of samples in the sample enrichment and pyrolysis chamber into the dilution chamber under certain conditions, a design of the positioning areas is convenient forconveniently and quickly positioning and fixing the microfluidic chip, the microfluidic chip is applied to a centrifugal microfluidic technology to realize a molecular diagnosis based on PCR amplification, and a whole reaction process is in a closed microfluidic chip structure.

The invention relates to a full-extraction molecular diagnosis microfluidic chip and a microfluidic system. The full-extraction molecular diagnosis microfluidic chip includes a storage cavity, a sample cavity, a first liquid adding cavity, a first buffer cavity, at least one controlled liquid adding cavity, a second liquid adding cavity, a distribution cavity, a first liquid waste cavity, a buffering cavity, a second liquid waste cavity, at least one measuring cavity and at least one PCR amplification cavity. The portions, near the rotating center of a target, of the controlled liquid adding cavities are communicated with the second liquid adding cavity through air pipelines, the air pipelines are internally provided with phase change valves, and the controlled liquid adding cavities are communicated with the distribution cavity through liquid adding pipelines. Sample splitting, nucleic acid purification, equal distribution of reagents, amplification of nucleic acids in the chambers under restrictions of specific primers and other processes are all integrated in the centrifugal microfluidic chip, subsequent analysis work such as fluorescence signal collection and analysis can be achieved, a single-sample multi-index diagnosis mode is achieved, and it is possible to screen multiple pathogens of diseases through molecular diagnosis.

A centrifugal microfluidic device is provided having a microfluidic circuit, a fluid reservoir for providing fluid in the microfluidic circuit, a hydrodynamic resistance element in fluid communication with the reservoir for controlling rate of flow of a fluid out of the reservoir, and a siphoned chamber in fluid communication with the hydrodynamic resistance element and the microfluidic circuit for receiving fluid from the hydrodynamic resistance element and for delaying and metering of the fluid into the microfluidic circuit. The microfluidic device is useful for performing a biological assay. Operation of the device is completely independent on the liquid-solid contact angle and wetting properties of the liquids on the solid material of the platform, and the device does not need a carefully controlled rotation protocol.

Provided are a centrifugal microfluidic device having a sample distribution structure and a centrifugal microfluidic system including the centrifugal microfluidic device. The centrifugal microfluidic device includes: a rotatable platform; a sample chamber which is disposed in the rotatable platform and houses a fluid sample; a distribution channel connected to an outlet of the sample chamber; a valve which is disposed in the outlet of the sample chamber; a plurality of non-vented reaction chambers which are disposed in the rotatable platform outside of the distribution channel in the radial direction; and a plurality of inlet channels connecting the distribution channel with the reaction chambers.

The invention provides a centrifuging micro-fluidic control chip for free nucleic acid extraction and a method for extracting free nucleic acid, and belongs to the technical field of free nucleic acidseparating and extracting devices. The centrifuging micro-fluidic control chip comprises a disc, plasma separating chambers, nucleic acid extraction sample cavities, immiscible phase cavities and eluting cavities, wherein the plasma separating chambers, the nucleic acid extraction sample cavities, the immiscible phase cavities and the eluting cavities are in rotary symmetric distribution; each plasma separating chamber communicates with the corresponding nucleic acid extraction sample cavity through a corresponding passive siphon; and each nucleic acid extraction sample cavity communicates with the corresponding immiscible phase cavity and the corresponding eluting cavity through arch capillary microchannels. According to the centrifuging micro-fluidic control chip disclosed by the invention, a dynamic centrifuging micro-fluidic control method and a static immiscible phase micro-fluidic control method are combined for the first time, and the designed immiscible phase nucleic acid extracting and purifying structure realizes uniform mixing under the premise that the interface stability is not influenced; the plasma separating structure is integrated on the centrifuging micro-fluidiccontrol chip, so that the purpose of extracting and purifying free nucleic acid from 4mL whole blood fully-automatically within 15 minutes can be realized, the centrifuging micro-fluidic control chipis the fastest device for extracting and purifying the free nucleic acid from the whole blood at present, and the centrifuging micro-fluidic control chip is hopefully applied to liquid biopsy of thefree nucleic acid.

The invention relates to a centrifugal microfluidic chip and a nucleic acid analysis system, wherein the centrifugal microfluidic chip comprises a chip substrate, a sample adding cavity, an air outlet, a sample enrichment cracking cavity, a first waste liquid cavity, a first capillary valve, a dilution cavity, a second capillary valve, a second waste liquid cavity and a plurality of PCR amplification cavities. According to the centrifugal microfluidic chip and the nucleic acid analysis system, through the ingeniously designed cavity structure, the PCR amplification technology can be applied tothe centrifugal microfluidic technology to achieve the molecular diagnosis based on the PCR amplification, and through the sequential control of the liquid in the sample adding cavity, the sample enrichment cracking cavity, the dilution cavity and the PCR amplification cavity, the PCR amplification is achieved in the PCR amplification cavity. The whole reaction process is in a closed microfluidicchip structure, so the burden of operators and the possibility of pollution are reduced, the whole molecular diagnosis process does not depend on a molecular diagnosis laboratory or on professional operators any more, thereby meeting the requirement of quick detection anytime and anywhere, and bringing great help for medical examinations and disease prevention and control.

The invention belongs to the technical field of micro-fluidic chips, and discloses a control valve arranged on a micro-fluidic channel (2) of a micro-fluidic chip. The micro-fluidic chip comprises a chip body (1), the control valve comprises a valve cavity (3) and a reversible water absorption mechanism (4), and the reversible water absorption mechanism (4) is arranged inside the valve cavity (3).The invention further discloses a centrifugal micro-fluidic chip comprising a valve body (1), a liquid inlet (7), a reaction cavity (8) and the micro-fluidic channel (2), wherein the centrifugal micro-fluidic chip further comprises a control valve arranged between the liquid inlet (7) and the reaction cavity (8), and the control valve is the control valve disclosed in the invention. According tothe control valve and the centrifugal micro-fluidic chip provided by the invention, the process is simple, complex processing is not needed, step-by-step release of a solution can be realized by changing the centrifugal rotational speed, and the control valve is convenient to use.

A centrifugal microfluidic chip mounting, kit and method include a swivel joint permitting a chip to rotate about an axis of the chip in a plane swept by a centrifuge, and a force applicator for controlling an angle of the swivel and for applying a force in proportion to a rotational rate of the centrifuge. The mounting includes: a blade part (18) that couples to, or defines, a blade (10) of a centrifuge at a radial distance from a centrifuge axis (12); a chip part (20) that holds the chip at an orientation having a normal not perpendicular to the axis; a one degree of freedom (DoF) joint (16) between the blade part and the chip part; and a force applicator (28) which bears on the chip part at a fixed set of one or more points, which do not surround, and are not surrounded by, the joint.

The invention provides a method for detecting small molecules in milk based on centrifugal microfluidics, and a special chip thereof. The whole detection method including pretreatment and test strip detection is completed through a centrifugal microfluidic system, manual operation is replaced by mechanical operation, the detection efficiency is greatly improved, the operation steps are simplified, and sources causing errors are reduced. Experiment steps can be effectively simplified, manual operation is replaced by mechanical operation, errors caused by manual operation are reduced, and the detection precision is improved. In the centrifugal microfluidic system, the microfluidic chip is used in cooperation with double-shaft centrifugal platform, the special structure of the microfluidic chip is matched with a rotating shaft and a centrifugal shaft of a double-shaft centrifugal platform, fluid is directionally driven, meanwhile, effective separation of magnetic beads and liquid can be achieved through the magnet, and in addition, the infrared light source heating function of the double-shaft centrifugal platform enables a liquid to be heated to release small molecules.

The invention relates to a liquid reagent storage box and a micro-fluidic chip structure. The storage box comprises a reagent box main body, a reagent box bottom plate and a sealing plug. The reagentbox bottom plate and the reagent box main body are matched with each other and form a storage cavity jointly. The position, away from the rotation center of the storage box, of the storage box is provided with an outlet. The outlet is located in the position, adjacent to the reagent box main body, of the reagent box bottom plate. The storage cavity communicates with the outlet and is used for communicating with the exterior through the outlet. The reagent box bottom plate is further provided with an outflow structure at the outlet. The outflow structure and other structures, adjacent to the outflow structure, of the reagent box bottom plate form a height difference. According to the liquid reagent storage box, on the one hand, through the design of the storage cavity, a liquid reagent needed by centrifugal micro-fluidic control can be stored conveniently effectively; and on the other hand, through the design of the height difference of the outflow structure at the outlet, the completeoutflow of liquid in the centrifugal process can be ensured, reagent waste or shortage is avoided, cost is reduced, and the reaction effect of centrifugal micro-fluidic control is ensured.

The invention, which belongs to the technical field of bacterial lysis devices, discloses a portable bacterial lysis device based on a centrifugal microfluidic technology and a using method thereof. The device comprises upper and lower circular plates, a disc micro-fluidic chip, and a rotating shaft, wherein the disc micro-fluidic chip and the rotating shaft are parallel to each other between theupper and lower circular plates. The rotating shaft passes through the parts, away from the circle centers, of the circulate plates but is not in contact with the circular plates; and the rotating shaft passes through the circle center of the disc micro-fluidic chip. A plurality of magnets are respectively arranged on the circular plates and are uniformly distributed on each circular plate, wherein the magnets are mutually staggered in a vertical position. A plurality of bacteria lysis pools are arranged on the disc micro-fluidic chip. According to the invention, the bacterial lysis efficiencyand the sample treatment flux can be effectively improved; and other operation units can be integrated to realize real-time on-site detection of bacteria. The designed bacterial lysis device is portable, low in cost, easy and convenient to operate; the operation is automatic; and the device is used flexibly. The portable bacterial lysis device can meet different requirements and has the broad application prospects.

The invention provides a chemiluminescence immunoassay disk based on a centrifugal microfluidics technology and a working method thereof. The chemiluminescence immunoassay disk based on the centrifugal microfluidics technology comprises a sample loading port, a first reagent port, a detecting groove and a waste liquid groove, and a valve, the valve controls communication between the detecting groove and the waste liquid groove, and comprises a meandering passage formed in the disk, a relationship between a minimum rotation radius R1 of the passage and a maximum rotation radius R2 of the detecting groove is that R1-R2 is less than 0. The chemiluminescence immunoassay disk based on the centrifugal microfluidics technology has the advantages of stability, reliability, efficiency, quickness and the like.

A microfluidic system for processing biological samples comprising a holding chamber adapted for holding a fluid and to be rotated on a platform, said holding chamber comprising an outlet through which fluid flow is controlled by an acceleration-primed valve system, wherein the acceleration-primed valve system comprises a capillary valve and an outlet channel. The invention provides a novel valving system, which retains fluids at low angular velocities, removes the need for hydrophilic surfaces, minimises disc real-estate and optimises certain microfluidic processes done in the holding chamber.

The invention belongs to the microfluidics field and discloses a clamping mechanism for a centrifugal microfluidics CD chip. The mechanism comprises chip support racks 1, a lever 2, a push rod 3, a positioning marble 4, a motor shaft clamping groove 5, a guide block 6 and a guide slot 7. The chip is inserted to the bottom along the guide slot 7 and can reach the accurate position by means of the horizontal positioning function of the guide block 6 and cooperation with the motor shaft clamping groove 5, and the chip is completely positioned when the positioning marble 4 in the motor shaft clamping groove 5 bounces; when the chip is required to be taken out, all that is required is to push the push rod 3 and the chip can automatically bounce under the action of the lever 2. The distance between the chip support racks 1 can be conveniently adjusted and thus the mechanism can adapt to chips with different sizes. The clamping mechanism for the centrifugal microfluidics CD chip is designed in the CD driver principle, can conveniently clamp and position the chip and has the advantages of being simple in structure, convenient to operate, reliable and high in adaptability.

The invention relates to a centrifugal microfluidic chip and a nucleic acid analysis system, wherein the centrifugal microfluidic chip includes a chip substrate, a magnetic adsorption component and amagnetic component; the chip substrate is also provided with a stirring chamber and a liquid injection hole connected with the stirring chamber; the magnetic adsorption component is accommodated in the stirring chamber; the chip substrate has a rotating center, and the magnetic component is arranged outside the chip substrate and is located at a position, close to the rotating center, of the stirring chamber. On one hand, when the magnetic adsorption component moves to a position away from the magnetic component, and under the action of centrifugal force, the magnetic adsorption component moves to the position, far from the rotating center, of the stirring chamber; on the other hand, when the magnetic adsorption component moves to a position close to the magnetic component, the magnetic adsorption component moves to a position, close to the rotating center, of the stirring chamber under the action of magnetic adsorption. An ideal mixing effect can be achieved, and a certain cracking effect is achieved and is stable and effective on the premise of simplifying the structure and needing no complicated instruments and equipment.

The invention relates to a controllable reagent container for centrifugal microfluidic control and a centrifugal microfluidic chip. The controllable reagent container comprises a container main body,wherein a reagent accommodating space is formed in the container main body and is provided with a release port, and an opening is formed in the reagent accommodating space; a top flange connected withthe container main body is further arranged at the opening and has a position protruding out of the container main body; and the container main body is provided with a diversion structure at the release port. The controllable reagent container has the advantages of simple structure, controllable reagent release function, suitability for batch preparation, convenience for rapid alignment and installation due to the design of the top flange, and convenience for use; firstly, reagents can be released in centrifugation, and the reagent container is applicable to both solid reagents and liquid reagents; secondly, the control of the reagent release interface can be realized in the centrifugal process without extra power or enabling, and the rotation of the chip does not need to be stopped; andfinally, various reagents can be contained, and the reagent releasing device is suitable for various steps of reagent releasing of POCT equipment.