37 results about "Hydrodynamic radius" patented technology

The invention belongs to the technical field of preparation of nano-materials, and discloses lignin nano-particles with high ultraviolet protection performance and a preparation method and application of the lignin nano-particles. The preparation method comprises the following steps: (1) dissolving 0.1-50 parts by weight of lignin in 1,000-2,000 parts by weight of an acetone / water mixed solvent to obtain a lignin organic solution; and (2) mixing the lignin organic solution in the step (1) with 1,000-4,000 parts by weight of water, and stirring to obtain the lignin nano-particles. The hydrodynamic radius of the lignin nano-particles is 50-300 nm, the lignin nano-particles are solid spherical particles of which hydrophilic groups are positioned externally and hydrophobic groups are positioned internally, the number of phenolic hydroxyl groups distributed on the unit area is large, the ultraviolet protection performance is the best, and the oxidation resistance is the most excellent. The size is controllable, the field of large-scale and high-value application of the lignin is expanded, and particularly, the lignin nano-particles which are used as ultraviolet protecting agents have huge application prospect in the field of high-end daily chemical products such as sun block and skin care.

System and method for loading the front line anticancer drug, doxorubicin (DOX) onto DNA-capped gold nanoparticles whose duplex DNA has been designed for specific DOX intercalation. Since each AuNP contains about 108 high affinity drug sites, this design allows for a high local DOX concentration on the particle. Drug binding was confirmed by monitoring the increase in DNA melting temperature, the shift in the plasmon resonance maximum, and the increase in the NP hydrodynamic radius as a function of [DOX] / [DNA] ratio. The feasibility of the nanoparticles as a drug delivery system was demonstrated by showing that particle-bound DOX could be transferred to a target DNA.

The invention relates to the field of pharmacology. More specific, the invention relates to a controlled release composition.This invention is related to a controlled release composition comprising a cross-linked gelatin and at least one therapeutic protein wherein the ratio of the average mesh size (ξ) of the gelatin matrix and the average hydrodynamic radius (RH) of the therapeutic protein is smaller than 2, preferably smaller than 1.5.

The invention discloses a high-fluidity high-strength high-toughness polypropylene composite material. The high-fluidity high-strength high-toughness polypropylene composite material comprises the following raw materials of: in percent by weight, 69-97% of polypropylene, 1-20% of inorganic nano filler, 1-30% of hyperbranched polymer and 0.1-1% of an antioxidant. Compared with traditional linear toughening agents, the hyperbranched polymer has a spatial spherical structure and a smaller hydrodynamic radius, and exhibits the characteristics of remarkable lubricity and low melt viscosity, so thatthe impact strength of the material is improved, and meanwhile the melt fluidity of PP and other polyolefin materials is improved greatly; in addition, a better property of dispersion of inorganic nanoparticles is achieved, and therefore a reinforcing effect of the inorganic nanoparticles on the material can be further achieved, so that the product has excellent performance especially in an inorganic nano-reinforced system.

The invention relates to the field of pharmacology. More specific, the invention relates to a controlled release composition.This invention is related to a controlled release composition comprising a cross-linked gelatin and at least one therapeutic protein wherein the ratio of the average mesh size (ξ) of the gelatin matrix and the average hydrodynamic radius (RH) of the therapeutic protein is smaller than 2, preferably smaller than 1.5.

This invention enables high throughput detection of small molecule effectors of particle association, as well as quantification of association constants, stoichiometry, and conformation. Given a set of particle solutions having different concentrations, dynamic light scattering measurements are used to determine the average hydrodynamic radius, as a function of concentration. The series of average hydrodynamic radii as a function of concentration are fitted with stoichiometric association models containing the parameters of molar mass, modeled concentrations, and modeled hydrodynamic radii of the associated complexes. In addition to the average hydrodynamic radii value analysis, the experimental data may be fit / analyzed in alternate ways. This method may be applied to a single species that is self-associating or to multiple species that are hetero-associating. This method may also be used to characterize and quantify the association between a modulator and the associating species.

The invention belongs to the technical field of nanomaterials and discloses a lignin / surfactant composite nanoparticle and a preparation method thereof. The preparation method comprises the followingsteps: (1) dispersing 100 parts by mass of lignin into alkaline liquid, adjusting the pH value of the system to 9-12, adding 10-25 parts by mass of epoxy chloropropane, carrying out cross-linking reaction at 80-95 DEG C for 1-3 hours, so as to obtain high molecular weight lignin; (2) adding high molecular weight lignin, alkali and a surfactant into water, so as to obtain a mixed solution with thepH value of 9-12; and (3) adding acid into the mixed solution until the pH value of the mixed solution is 4-6, and stirring, so as to obtain a water dispersion solution of the lignin / surfactant composite nanoparticle. The lignin / surfactant composite nanoparticle prepared by virtue of the preparation method has excellent water dispersion performance, the hydrodynamic radius is 40nm-500nm, and the lignin / surfactant composite nanoparticle can be very stably dispersed into water and can be easily mixed with other components.

This invention enables high throughput detection of small molecule effectors of particle association, as well as quantification of association constants, stoichiometry, and conformation. “Particle” refers to any discrete particle, such as a protein, nucleic acid, carbohydrate, liposome, virus, synthesized polymer, nanoparticle, colloid, latex sphere, etc. Given a set of particle solutions having different concentrations, dynamic light scattering measurements are used to determine the average hydrodynamic radius, ravg, as a function of concentration. The series of ravg as a function of concentration are fitted with stoichiometric association models containing the parameters of molar mass, modeled concentrations, and modeled hydrodynamic radii of the associated complexes. In addition to the ravg value analysis, the experimental data may be fit / analyzed in alternate ways. This method may be applied to a single species that is self-associating or to multiple species that are hetero-associating. This method may also be used to characterize and quantify the association between a modulator and the associating species.

The invention provides a PEGlated lipid material, and a preparation method and an application thereof. The material has a structure represented by formula (I). The material has oxidation responsiveness, and can be used as a drug carrier. An experiment result shows that the material is amphiphilic, undergoes phenylboronate oxidation removal in the presence of H2O2, and further undergoes a quinine-methide rearrangement reaction to realize the dissociation of a hydrophilic chain segment and a hydrophobic chain segment of the PEGlated lipid material; and the material entraps celecoxib and is self-assembled to form nano-micelles, the particle size of nano-particles is about 90 nm, and the hydrodynamic radius of the nano-micelles is 41.5-44.5 nm; and the entrapping rate is 54%, and the drug loading capacity is 5.4%.

This invention enables high throughput detection of small molecule effectors of particle association, as well as quantification of association constants, stoichiometry, and conformation. “Particle” refers to any discrete particle, such as a protein, nucleic acid, carbohydrate, liposome, virus, synthesized polymer, nanoparticle, colloid, latex sphere, etc. Given a set of particle solutions having different concentrations, dynamic light scattering measurements are used to determine the average hydrodynamic radius, ravg, as a function of concentration. The series of ravg as a function of concentration are fitted with stoichiometric association models containing the parameters of molar mass, modeled concentrations, and modeled hydrodynamic radii of the associated complexes. In addition to the ravg value analysis, the experimental data may be fit / analyzed in alternate ways. This method may be applied to a single species that is self-associating or to multiple species that are hetero-associating. This method may also be used to characterize and quantify the association between a modulator and the associating species.

The invention provides a hyper-branched macromolecular flocculation demulsifying agent, which has the characteristics of many terminated functional group, high dissolubility and low viscosity. The hyper-branched macromolecular flocculation demulsifying agent is not liable to bend in a saline medium and has large hydrodynamic radius, high flocculation capability and high flocculation speed. Meantime, the hyper-branched macromolecular flocculation demulsifying agent has a good demulsifying effect on oil-water emulsion due to the branched structure and high electric charge density.

The invention provides a polymer capable of releasing SO2, a preparation method and an application and nano-micell. The polymer has a structure shown in a formula I, the polymer material has amphipathy, and can be existed under cysteine or glutathione environment, a dinitrobenzene sulfonamide group in a formula I responses to a mercapto group, and is subjected to a rearrangement reaction to release SO2, and the material is subjected to self assembly in an aqueous solution to form the nano-micell. The polymer has good water dispersion performance, and can be endocytosed by cells, is capable ofreleasing SO2 under effect of glutathione in the cells, increases the active oxygen level in the cancer cells, and presents obvious killing effect for cancer cells. The polymer nanoparticles can reduce the cancer cell survival rate to 11+ / -1%, and the hydrodynamic radius of the nanoparticles is 29+ / -0.1 nm.

There is provided a method for assessing the quality of platelet concentrates for determining their suitability for transfusion. More specifically dynamic light scattering measurements of the samples containing platelets are obtained and parameters, such as hydrodynamic radius and relative number of platelet-derived microparticles, are derived from these measurements that are indicative of platelet quality.

The invention provides a preparation method of a pH response graft copolymer based on methacrylic acid amino ester. The preparation method comprises the following steps: firstly dissolving methacrylic acid macrogol ester and methacrylic acid amino ester in a solvent; adding a divinyl cross-linking agent; taking a thiols compound as a chain transfer agent, and triggering a polymerization reaction with a radical initiator; controlling the molar ratio of the cross-linking agent to the chain transfer agent to control the reaction so as to obtain the graft copolymer. When the pH of the graft copolymer is smaller than 6, aggregates with the hydrodynamic radius of 130-140 nm are aggregated; when the pH of the graft copolymer is about 7, the shapes of the aggregates change suddenly; and when the pH of the graft copolymer is greater than 8, the hydrodynamic radius of the aggregates becomes about 20 nm, and good pH response performance is represented. Moreover, the reactivity site points of amino groups, and carboxyl groups and the like on the surface of the graft copolymer are easily subjected to macromolecular modification, functional groups are introduced, and the graft copolymer has a wide application prospect in the fields of materials science, medical science, biology and the like.

The invention relates transiently attaching drag-tags to molecules during electrophoresis. The invention includes running buffers having drag-tags that transiently attach to lipophilic moieties attached to the molecules. The lipophilic moieties can be covalently or ionically bonded to the molecules. One particular aspect of the invention is a nucleoside analog or a nucleic acid analog comprising a lipophilic moiety. The invention is also directed to methods of separating molecules that comprise a lipophilic moiety. The methods generally comprise transiently attaching a drag-tag to the lipophilic moiety during a separation modality. These methods can be used to separate the molecules by size or weight, to measure a hydrodynamic radius of a drag-tag, or to separate a plurality of drag-tag by their hydrodynamic radius.

This invention provides a method for discriminating a liquid crystal alignment agent having coating defect generating possibility before the liquid crystal alignment agent is applied and to provide the liquid crystal alignment agent causing no coating defect using the same. In an evaluation method of an organic macromolecular polymer solution, printing properties when the organic macromolecular polymer solution is used as a liquid crystal alignment agent are evaluated by measuring dynamic light scattering of the organic macromolecular polymer solution and obtaining the maximum size or the position of a peak top of a flocculation body originating from an organic macromolecular polymer component from the measured dynamic light scattering. The liquid crystal alignment agent has <=20 [mu]m peak top hydrodynamic radius (RH) of the maximum peak or an average value thereof of a flocculation body originating from the organic macromolecular polymer component.

The invention discloses a method for detecting an acetate cellosilk bundle. The detection method is used for detecting a structure of the acetate cellosilk bundle by a light scattering method. The method comprises the following steps of S1, dissolving the acetate cellosilk bundle into acetone, performing ultracentrifugation, taking filtrate, and performing membrane filtration; S2, detecting the filtrate obtained by membrane filtration in the S1 by the light scattering method to obtain a fluid mechanics radius Rh, a mean square rotation radius Rg and a structural factor Rg / Rh; and S3, determining the existence state of the cellosilk bundle in an acetone solution to be a single chain, micelle or a micelle cluster according to the fluid mechanics radius Rh and a fluid mechanics radius distribution graph. The detection method can acquire detailed structural information of a polymer in the solution and has a great significance in synthesis and spinning of the tobacco acetate cellosilk bundle. According to the detection method disclosed by the invention, spinning processing of the acetate cellosilk bundle is instructed, and blocking in a processing process can be avoided.

An amphiphilic comb-shaped superplasticizer is disclosed. In order to study the effect of hydrophobic long side chains on the dispersibility of comb superplasticizers, an amphiphilic comb-shaped poly(dodecyl methacrylate)-isoprenol polyoxyethylene ether-maleic anhydride terpolymer (APC) is prepared by an aqueous solution free radical copolymerization by use of ammonium persulfate as an initiator.Comb type. Three monomers are well copolymerized. The weight average relative molecular mass and the number average relative molecular mass of a sample are 50625 and 10173, respectively. The relativemolecular mass distribution index is 4.97. Through introduction of a hydrophobic alkyl ester long side chain, the comb copolymer is self-assembled in a cement pore solution to form micelles with a diameter of more than 100 nm. A TEM (transmission electron microscope) observes the micelles to find that the micelles are spherical and cemented together to form agglomerates, the hydrodynamic radius and the solvation layer thickness of the copolymer can be increased, and the dispersion properties of a water reducer can be reduced.

The invention discloses a layered double-metal hydroxide composite material, a preparation method and application thereof. The layered double-metal hydroxide composite material comprises a layered double-metal hydroxide and rare earth metal cations, wherein the surface of the layered double-metal hydroxide is modified with a carboxyl-containing organic ligand, the rare earth metal cations are coordinated with the carboxyl-containing organic ligand, the molecular weight of the carboxylic acid-containing organic ligand is greater than 1000, and the hydrodynamic radius of the carboxylic acid-containing organic ligand is greater than the interlayer distance of the layered double-metal hydroxide. According to the invention, the layered double-metal hydroxide is used as an inorganic matrix, the specific carboxyl-containing organic ligand and the layered double-metal hydroxide are integrated into a whole through assembly, and the novel rare earth organic-inorganic hybrid material is obtained by utilizing the advantage of good coordination binding property of the carboxyl-containing organic ligand and rare earth metal cations, so that the stability is enhanced, and the application range of fluorescence analysis is expanded, and the detection limit is good.

The invention discloses a preparation method of a salt-resistant stable thickener material, and belongs to the technical field of food additive materials. In the technical solution of the present invention, a spatial network structure formed by association of long-chain monomers has high resistance to inorganic salts and temperature damage. Meanwhile, according to the technical solution of the present invention, polyacrylic acid material is used as a main raw material. Due to the spatial network structure and introduction of the hydrophobic long-chain monomers to reinforce the spatial structure, the polymer has higher hydrodynamic radius, water-absorbing ability and salt water resistance, thereby improving electrolyte resistance of a thickener. Meanwhile, during the polymerization of the thickener, a small amount of long-chain monomers with hydrophilic groups are copolymerized with the polymerized monomers. Due to the spatial network structure with a certain crosslinking degree in thepolymer itself and introduction of the hydrophobic long-chain monomers, the spatial network structure is enhanced, so that the polymer has higher hydrodynamic volume, water absorption and retention capacities.

Method for recovering a target protein from a feedstock comprising said target protein and at least one impurity compound selected from host cell proteins (HCP), DNA, RNA or other nucleic acid, the target protein being characterized by a hydrodynamic radius Rh1 and the impurity compound being characterized by a hydrodynamic radius Rh2, wherein Rh1>Rh2, comprising the following steps (i) to (iv) and optionally step (v): (i) providing a polymeric mesh comprising at least one crosslinked polymer containing positively charged amino groups, wherein the polymer has a pore size exclusion limit Rhi which can be set variably; (ii) adapting the variable pore size exclusion limit Rhi of the polymeric mesh such that Rh2<Rhi and Rh1>Rhi; (iii) contacting the polymeric mesh with the feedstock; (iv) separating the polymeric mesh containing the retained impurity compound from the feedstock containing the excluded target protein.

The invention discloses a method for detecting an acetate cellosilk bundle. The detection method is used for detecting a structure of the acetate cellosilk bundle by a light scattering method. The method comprises the following steps of S1, dissolving the acetate cellosilk bundle into acetone, performing ultracentrifugation, taking filtrate, and performing membrane filtration; S2, detecting the filtrate obtained by membrane filtration in the S1 by the light scattering method to obtain a fluid mechanics radius Rh, a mean square rotation radius Rg and a structural factor Rg / Rh; and S3, determining the existence state of the cellosilk bundle in an acetone solution to be a single chain, micelle or a micelle cluster according to the fluid mechanics radius Rh and a fluid mechanics radius distribution graph. The detection method can acquire detailed structural information of a polymer in the solution and has a great significance in synthesis and spinning of the tobacco acetate cellosilk bundle. According to the detection method disclosed by the invention, spinning processing of the acetate cellosilk bundle is instructed, and blocking in a processing process can be avoided.

The invention discloses a composition for maintaining the stability of the residual polymer in polymer flooding produced water, wherein the composition comprises, by mass concentration, 50-200 mg / L aninhibitor for emulsified oil-induced influence, 100-150 mg / L an inhibitor for solid suspended matter-induced influence, and 50-100 mg / L an inhibitor for metal ion-induced influence, wherein the inhibitor for emulsified oil-induced influence is selected from AEO-7 and OP-10, the inhibitor for solid suspended matter-induced influence is selected from sodium silicate, sodium carboxymethyl celluloseand polyvinyl alcohol, and the inhibitor for metal ion-induced influence is selected from ethylenediaminetetraacetic acid disodium salt and sodium citrate. The invention further discloses applicationsof the composition. According to the present invention, after the composition is added to the polymer flooding produced water, the stability of the residual polymer can be enhanced, such that the subsequent treatment can be conveniently performed, the residual polymer in the polymer flooding produced water is not easily aggregated, the hydrodynamic radius remains the unchanged state within 24 h,and the passing ability of the micropores is good.

A hydrogel particle that has an average cross-sectional diameter in the range from 1 micrometer (μm) to 1000 μm, wherein the particle includes a first polymer network with an average mesh size that allows diffusion of a molecule with an hydrodynamic radius of 1000 nanometer (nm) or less into the first polymer network and which particle includes one or more binding molecules that are immobilized by the polymer network. The hydrogel particle preferably has wherein the first polymer network has an average mesh size that prevents diffusion of a molecule with an average hydrodynamic radius of more than 1000 nm to diffuse into the first polymer network, preferably the mesh size prevents diffusion of a molecule with an average hydrodynamic radius of more than 100 nm, and preferably more than 5 nm. Methods for reducing the bioavailability of one or more soluble biological molecules in a biological system by using the described hydrogel particle.

The invention provides a hyper-branched macromolecular flocculation demulsifying agent, which has the characteristics of many terminated functional group, high dissolubility and low viscosity. The hyper-branched macromolecular flocculation demulsifying agent is not liable to bend in a saline medium and has large hydrodynamic radius, high flocculation capability and high flocculation speed. Meantime, the hyper-branched macromolecular flocculation demulsifying agent has a good demulsifying effect on oil-water emulsion due to the branched structure and high electric charge density.

Disclosed are systems and methods for estimating the vitreous half-life of a therapeutic agent. In particular, systems and methods are disclosed for predicting the vitreous half-life of a therapeuticagent conjugated to a polymer that make use of an empirically-derived relationship of vitreous half-life to the hydrodynamic radius of a candidate therapeutic agent-polymer conjugate. The present disclosure is further directed to the use of the systems and methods disclosed herein to design a candidate therapeutic agent-polymer conjugate with a preselected vitreous half-life.

The present invention provides a releasable SO 2 The polymer, its preparation method and its application and nano micelles, the polymer has the structure of formula I. The polymer material has amphiphilicity, and in the environment where cysteine ​​or glutathione exists, the dinitrobenzenesulfonamide group in the structure of formula I responds to the sulfhydryl group and undergoes a rearrangement reaction to release SO 2 ; The material self-assembles in aqueous solution to form nanomicelles. The releasable SO provided by the present invention 2 The polymer has good water dispersibility, can be endocytosed by cells, and releases SO under the action of intracellular glutathione 2 , increase the level of reactive oxygen species in cancer cells, and then show a significant killing effect on cancer cells. Releasable SO 2 The polymer nanoparticles were able to reduce the cancer cell survival rate to 11±1%, and the hydrodynamic radius of the nanoparticles was 29±0.1nm.

The invention relates transiently attaching drag-tags to molecules during electrophoresis. The invention includes running buffers having drag-tags that transiently attach to lipophilic moieties attached to the molecules. The lipophilic moieties can be covalently or ionically bonded to the molecules. One particular aspect of the invention is a nucleoside analog or a nucleic acid analog comprising a lipophilic moiety. The invention is also directed to methods of separating molecules that comprise a lipophilic moiety. The methods generally comprise transiently attaching a drag-tag to the lipophilic moiety during a separation modality. These methods can be used to separate the molecules by size or weight, to measure a hydrodynamic radius of a drag-tag, or to separate a plurality of drag-tag by their hydrodynamic radius.

The invention relates to the technical field of polymers, in particular to a photoresponsive polymer microgel particle and a preparation method thereof. In the present invention, an azobenzene group that can undergo isomerization under light irradiation is chemically bonded to poly(N-isopropylacrylamide-acrylic acid) copolymer microgel to prepare azobenzene Modified poly(N-isopropylacrylamide-acrylic acid) copolymer microgel, the copolymer microgel of the prepared product has a good photoresponse effect, and the weight-average hydrodynamic radius of the microgel particles is at a wavelength of 360 nanometers of ultraviolet light After irradiation, an increase of more than 20% occurs, and the weight-average hydrodynamic radius of the microgel particles can return to the original size after irradiation with visible light with a wavelength of 465 nanometers. The preparation method of the invention has simple experimental operation and easy control, simple experimental equipment and process method, and low cost.

The invention discloses an oil displacement binary composite system formula optimization method based on pore throat radius suitability, which comprises the following steps: S1, preparing a series of polymer-surfactant / alkali binary composite system solutions with different polymer concentrations and surfactant / alkali concentrations, and measuring the hydrodynamic radius Rh of each composite system; S2, determining a minimum pore throat radius Rm matched with the hydrodynamic radius Rh according to a bridging theory, and drawing a polymer concentration-surfactant concentration / alkali concentration-minimum pore throat radius plate; S3, drawing a polymer concentration-surfactant concentration / alkali concentration-minimum pore throat radius plate for different polymers; and S4, according to the pore throat radius of the target oil reservoir, comparing the polymer concentration-surfactant concentration / alkali concentration-minimum pore throat radius plate, considering the viscosity of the composite system, and optimizing and selecting a polymer-surfactant / alkali composite system formula. According to the method, the optimal formula of the surfactant / alkali and the polymer can be quickly and effectively optimized.