37 results about "Hydrodynamic radius" patented technology

The invention relates to the field of pharmacology. More specific, the invention relates to a controlled release composition.This invention is related to a controlled release composition comprising a cross-linked gelatin and at least one therapeutic protein wherein the ratio of the average mesh size (ξ) of the gelatin matrix and the average hydrodynamic radius (RH) of the therapeutic protein is smaller than 2, preferably smaller than 1.5.

This invention enables high throughput detection of small molecule effectors of particle association, as well as quantification of association constants, stoichiometry, and conformation. “Particle” refers to any discrete particle, such as a protein, nucleic acid, carbohydrate, liposome, virus, synthesized polymer, nanoparticle, colloid, latex sphere, etc. Given a set of particle solutions having different concentrations, dynamic light scattering measurements are used to determine the average hydrodynamic radius, ravg, as a function of concentration. The series of ravg as a function of concentration are fitted with stoichiometric association models containing the parameters of molar mass, modeled concentrations, and modeled hydrodynamic radii of the associated complexes. In addition to the ravg value analysis, the experimental data may be fit / analyzed in alternate ways. This method may be applied to a single species that is self-associating or to multiple species that are hetero-associating. This method may also be used to characterize and quantify the association between a modulator and the associating species.

There is provided a method for assessing the quality of platelet concentrates for determining their suitability for transfusion. More specifically dynamic light scattering measurements of the samples containing platelets are obtained and parameters, such as hydrodynamic radius and relative number of platelet-derived microparticles, are derived from these measurements that are indicative of platelet quality.

The invention discloses a method for detecting an acetate cellosilk bundle. The detection method is used for detecting a structure of the acetate cellosilk bundle by a light scattering method. The method comprises the following steps of S1, dissolving the acetate cellosilk bundle into acetone, performing ultracentrifugation, taking filtrate, and performing membrane filtration; S2, detecting the filtrate obtained by membrane filtration in the S1 by the light scattering method to obtain a fluid mechanics radius Rh, a mean square rotation radius Rg and a structural factor Rg / Rh; and S3, determining the existence state of the cellosilk bundle in an acetone solution to be a single chain, micelle or a micelle cluster according to the fluid mechanics radius Rh and a fluid mechanics radius distribution graph. The detection method can acquire detailed structural information of a polymer in the solution and has a great significance in synthesis and spinning of the tobacco acetate cellosilk bundle. According to the detection method disclosed by the invention, spinning processing of the acetate cellosilk bundle is instructed, and blocking in a processing process can be avoided.

A hydrogel particle that has an average cross-sectional diameter in the range from 1 micrometer (μm) to 1000 μm, wherein the particle includes a first polymer network with an average mesh size that allows diffusion of a molecule with an hydrodynamic radius of 1000 nanometer (nm) or less into the first polymer network and which particle includes one or more binding molecules that are immobilized by the polymer network. The hydrogel particle preferably has wherein the first polymer network has an average mesh size that prevents diffusion of a molecule with an average hydrodynamic radius of more than 1000 nm to diffuse into the first polymer network, preferably the mesh size prevents diffusion of a molecule with an average hydrodynamic radius of more than 100 nm, and preferably more than 5 nm. Methods for reducing the bioavailability of one or more soluble biological molecules in a biological system by using the described hydrogel particle.

The invention provides a hyper-branched macromolecular flocculation demulsifying agent, which has the characteristics of many terminated functional group, high dissolubility and low viscosity. The hyper-branched macromolecular flocculation demulsifying agent is not liable to bend in a saline medium and has large hydrodynamic radius, high flocculation capability and high flocculation speed. Meantime, the hyper-branched macromolecular flocculation demulsifying agent has a good demulsifying effect on oil-water emulsion due to the branched structure and high electric charge density.

Disclosed are systems and methods for estimating the vitreous half-life of a therapeutic agent. In particular, systems and methods are disclosed for predicting the vitreous half-life of a therapeuticagent conjugated to a polymer that make use of an empirically-derived relationship of vitreous half-life to the hydrodynamic radius of a candidate therapeutic agent-polymer conjugate. The present disclosure is further directed to the use of the systems and methods disclosed herein to design a candidate therapeutic agent-polymer conjugate with a preselected vitreous half-life.

The present invention provides a releasable SO 2 The polymer, its preparation method and its application and nano micelles, the polymer has the structure of formula I. The polymer material has amphiphilicity, and in the environment where cysteine ​​or glutathione exists, the dinitrobenzenesulfonamide group in the structure of formula I responds to the sulfhydryl group and undergoes a rearrangement reaction to release SO 2 ; The material self-assembles in aqueous solution to form nanomicelles. The releasable SO provided by the present invention 2 The polymer has good water dispersibility, can be endocytosed by cells, and releases SO under the action of intracellular glutathione 2 , increase the level of reactive oxygen species in cancer cells, and then show a significant killing effect on cancer cells. Releasable SO 2 The polymer nanoparticles were able to reduce the cancer cell survival rate to 11±1%, and the hydrodynamic radius of the nanoparticles was 29±0.1nm.

The invention relates transiently attaching drag-tags to molecules during electrophoresis. The invention includes running buffers having drag-tags that transiently attach to lipophilic moieties attached to the molecules. The lipophilic moieties can be covalently or ionically bonded to the molecules. One particular aspect of the invention is a nucleoside analog or a nucleic acid analog comprising a lipophilic moiety. The invention is also directed to methods of separating molecules that comprise a lipophilic moiety. The methods generally comprise transiently attaching a drag-tag to the lipophilic moiety during a separation modality. These methods can be used to separate the molecules by size or weight, to measure a hydrodynamic radius of a drag-tag, or to separate a plurality of drag-tag by their hydrodynamic radius.

The invention relates to the technical field of polymers, in particular to a photoresponsive polymer microgel particle and a preparation method thereof. In the present invention, an azobenzene group that can undergo isomerization under light irradiation is chemically bonded to poly(N-isopropylacrylamide-acrylic acid) copolymer microgel to prepare azobenzene Modified poly(N-isopropylacrylamide-acrylic acid) copolymer microgel, the copolymer microgel of the prepared product has a good photoresponse effect, and the weight-average hydrodynamic radius of the microgel particles is at a wavelength of 360 nanometers of ultraviolet light After irradiation, an increase of more than 20% occurs, and the weight-average hydrodynamic radius of the microgel particles can return to the original size after irradiation with visible light with a wavelength of 465 nanometers. The preparation method of the invention has simple experimental operation and easy control, simple experimental equipment and process method, and low cost.