4 results about "Hydrodynamic radius" patented technology

A method for determining binding parameters between a first particle (L) and a second particle (A) capable of non-covalently interacting with the first particle. The method includes acquiring N sets of raw data, each set of raw data acquired by performing a Taylor dispersion determination, and processing each of at least one set of raw data. Each Taylor dispersion determination for acquiring the corresponding set of raw data includes i) supplying a liquid portion comprising a sample portion containing the second particle (A) and at least one supplementary portion into a microfluidic channel, such that the sample portion and at least one supplementary portion form an interfacial contact in the microfluidic channel, wherein at least one of the liquid portions comprises a certain concentration of the first particle (L); ii) supplying the liquid portion to a flow in the microfluidic channel; and iii) acquiring the set of raw data, which includes reading a marked signal intensity s(t) as a function of time. Each set of raw data includes at least one imbalance readout, and processing each of the N sets of raw data includes determining the apparent diffusivity D and the hydrodynamic radius R. h At least one of them, and thus determine and will be the fitting equation for each of the N sets of original data.

The application relates to the field of material analysis, and discloses a test method for analyzing the properties of lotus plumule extract, which comprises the following steps: introducing the lotus plumule extract to be detected into a flow detection pool to establish a transient electrically induced birefringence monitoring field; applying a high-frequency bipolar symmetric square wave electric field to an excitation electrode pair; using the polarity high-frequency switching characteristic to limit the displacement of free ions within a local perturbation range, and simultaneously inducing the electric dipole orientation arrangement of alkaloid molecules; removing the electric field; monitoring and recording the transient birefringence decay trajectory data; and processing the data to separate the spin relaxation time constants belonging to different components, and determining the contents of nuciferine, isonor-lausplumuline and methylnuciferine according to the mapping rule between the constants and the effective hydrodynamic radius of molecules. The application realizes the physical decoupling of overlapping signals in the time domain through the spin relaxation characteristic, effectively suppresses the ionization interference of a complex matrix, and ensures the stability of a detection baseline.

The application discloses an anti-tumor immunotherapy nanoparticle and a preparation method and application thereof, relates to the technical field of nano-preparations, and has the technical scheme as follows: a core-shell structure includes two parts of a core and a shell; the core is a manganese oxide nanoparticle; the shell is a high-molecular polymer carrying a sound-sensitive agent; the hydrodynamic radius of the manganese oxide nanoparticle is 179.0+ / -10.8 nm, and the surface potential is 9.45+ / -1.73 mV; and the manganese oxide adjuvant is further included, wherein the manganese oxide adjuvant is a manganese dioxide nanoparticle, the sound-sensitive agent is one of porphyrin and derivatives thereof; the high-molecular polymer is a polylactic acid-glycolic acid copolymer; the particle size of the manganese dioxide nanoparticle is 20.7+ / -2.5 nm, and the surface potential is 17.34+ / -0.66 mV; and the effect is to provide a simple and effective strategy for the individualized immunotherapy of tumors.

A microfluidic cartridge and associated apparatus for performing exchange of buffers or concentrating biologics in liquid are disclosed. The cartridge may include a liquid transport mechanism, a sample container, a series of concentration measurement chambers, and air pressure driven valves for flow control. The cartridge may have a port through which the sample is transported to viscosity, static light scattering, and / or dynamic light scattering measuring devices in sequence for measurement of interaction parameter, molecular weight, hydrodynamic radius, opalescence, viscosity, and / or osmolality.