40 results about "Multiple species" patented technology

The invention provides a universal method for preparing animal tissue mononuclear suspension. A universal solution is provided for preparation of animal tissue single cell nucleuses, the method is suitable for different tissue types of various species, and the problem of unstable extraction caused by tissue differences in a traditional method is solved. Specifically, through multi-stage grinding and three dynamic quality inspection feedback mechanisms, hardness and cracking difficulty of different tissues are adapted, redundant operation is reduced to avoid damage of nuclear membranes caused by excessive grinding or excessive purification, nuclear membrane integrity and RNA stability are ensured, and extracted cell nucleuses are good in state and relatively high in integrity and can meet the requirement of single cell sequencing.

The invention relates to dsRNA capable of simultaneously preventing and treating various thrips and application of the dsRNA. Specifically, the invention provides a dsRNA construct, the construct of the dsRNA is a double strand, and the construct of the dsRNA comprises a nucleotide sequence of an insect nymph and / or adult stage regulation related gene or fragment; wherein the insect nymph and / or adult stage regulation related gene is an Actin gene, and the dsRNA is obtained by amplifying a sequence as shown in SEQ ID NO: 9-10. The fusion type dsRNA is designed to target a plurality of conservative thysanoptera actin gene dsRNA, so that the multi-killing effect is achieved, and the prevention and treatment effect of the fusion type dsRNA is equivalent to that of spinetoram which is commonly used in the market at present.

The invention discloses a conservative non-coding element identification method and system based on multi-species genome alignment, and the method comprises the following steps: establishing an index database based on reference genome sequences of multiple species, completing whole genome alignment, and further processing to obtain a high-credibility chain alignment result; the multi-species chain type comparison results are integrated into multi-sequence comparison data in a unified format; on this basis, a neutral evolution model is constructed based on quadruple degenerate sites, and candidate conservative regions are predicted through conservative scoring; in combination with genome annotation information, a length threshold is set, and a coding region and a UTR region are rejected, so that a high-confidence non-coding conservative element is obtained; and finally, displaying a cross-species conservative distribution diagram of the CNE by utilizing a visual tool. According to the method, the CNE with a potential regulation function can be accurately, efficiently and automatically identified, and technical support is provided for regulation system analysis, functional gene mining and molecular breeding of various organisms.

The invention discloses a fishway hydraulic power and fishway structure design method and related equipment, and the method comprises the steps: obtaining swimming capability parameters of each target fish species in a target water area, the swimming capability parameters comprising an induction flow rate, a critical swimming speed and an outbreak swimming speed; selecting the target fingerling with the minimum critical swimming speed as a limited fingerling; according to the critical swimming speed corresponding to the limited fingerling, calculating a first energy consumption rate of swimming of the limited fingerling at the first hydraulic flow speed; according to the first energy consumption rate, hydraulic function partitions and space sequence layout of the fishway are determined; and determining fishway hydraulic parameters according to the induction flow velocity of each target fingerling and the critical swimming speed and the outbreak swimming speed of the limited fingerling. According to the fishway, inclusive passing of the fishway to multiple species can be achieved, the structural design of the fishway can adapt to the natural behavior rhythm of fishes, the overall energy consumption of dam passing of fish schools is remarkably reduced, then the ecological efficiency of the fishway is improved, and the fishway can be widely applied to the technical field of fishway design.

The invention discloses dsRNA / fused dsRNA and application of the dsRNA / fused dsRNA in prevention and treatment of spider mites, the dsRNA is dsRpt3, and the fused dsRNA is obtained by fusing dsRNA of a plurality of genes of the same gene family of targeted spider mites; the gene family is selected from a proteasome ATP enzyme family and an apolipoprotein family; the nucleotide sequence of one chain of the fused dsRNA is as shown in any one of SEQ ID NO.1-4. The invention also discloses a preparation method of the fused dsRNA. Compared with the existing dsRNA for preventing and treating a single variety of spider mites, the dsRNA / fused dsRNA can prevent and treat various spider mites at the same time, is expected to be applied to preparation of biopesticides for preventing and treating spider mites, reduces the use of chemical pesticides, protects the environment, and reduces the damage of the chemical pesticides.

This invention discloses a genetic transformation and gene editing method applicable to multiple sesquiterpene species, belonging to the field of plant genetic transformation and gene editing technology. The method uses the cotyledonary nodes of sterile seedlings of the sesquiterpene genus as explants, and introduces an optimized pScEF1α-Cas9 gene editing transformation vector using Agrobacterium infection. Regenerated plants are obtained through recovery culture, selection culture, and rooting culture. The vector contains four gene expression units: pScEF1α:Cas9, pGmU6:sgRNA, pGmUBI:DsRed2, and pCaMV35S:HygR. In vivo screening is further performed using the DsRed2 fluorescent reporter gene, and the gene-edited plants are identified by molecular detection. This method is applicable to common sesquiterpene, stem-nodled sesquiterpene, and spiny sesquiterpene, and has advantages such as strong species versatility, high transformation efficiency, short cycle, visualized screening, and high positive rate, providing efficient technical support for gene function research and genetic improvement of sesquiterpene species.

The invention discloses a chromosome detection method for multi-species organisms, which comprises the following steps: inputting a to-be-detected image and prompt text information corresponding to species and tissue sources of the species into a trained visual language joint detection model, generating a structured detection result through reasoning of the trained visual language joint detection model, and determining the chromosome detection result according to the structured detection result. The structured detection result at least comprises target image position information, target category information and target text description information; the visual language joint detection model comprises a visual detection model used for carrying out target detection based on an image, and a multi-modal large language model used for generating target text description information based on prompt information and visual features from the visual detection model; the structured detection result is a fusion result of a target detection result from the visual detection model and target text description information from the multi-modal large language model. According to the invention, the accuracy of chromosome detection of multi-species organisms is improved.

A system and method for outbreak analysis by performing a taxonomic classification of the first LRS data to assign one or more taxonomic identifiers to each record of the first LRS data, identifying a plurality of species-specific candidate genomes from a reference genome database based on the assigned taxonomic identifiers, aligning the first LRS data with each of the plurality of species-specific candidate genomes to determine a first species identity of a microbial species present in the sample, and identifying a first closest strain of the identified microbial species present in the first sample by aligning the first LRS data with a plurality of strain-specific candidate genome datasets.

The application discloses a construction method of a multiplex PCR reaction system for detecting and identifying Podosphaera species and application thereof. The method comprises the following steps: obtaining whole genome sequences of multiple Podosphaera species, and performing multiple sequence alignment to obtain specific genes and specific gene clusters of each species; taking the genes in the specific gene clusters of each species as templates, designing multiple upstream primers and downstream primers according to a conventional primer design method to generate primer pairs, and excluding unreasonable primer pairs and primer pairs with low sensitivity and specificity; according to the size of the amplification products, the primers are freely combined and matched to generate four pairs of mixed multiplex primers, and the multiplex PCR reaction conditions are optimized to obtain a multiplex PCR system capable of specifically detecting Podosphaera and simultaneously identifying single and multiple species. The multiplex PCR method for detecting and identifying Podosphaera provided by the application can quickly and accurately complete the detection of Podosphaera and identify the species identity of Podosphaera.

The invention relates to an efficient plant genetic transformation vector based on rhizobium rhizogenes, which is modified on the basis of a pTi vector, and a left boundary pTi-LB and a right boundary pTi-RB of T-DNA of the vector are respectively replaced by a left boundary pRi-LB and a right boundary pRi-RB; deleting a Hyg expression cassette in the T-DNA region, and connecting a target gene expression cassette to the front end of the original RUBY expression cassette; a VirGN54D insert element is inserted between an Ori element and an SmR element in a non-T-DNA (Transfer-Deoxyribose Nucleic Acid) region; the 106th site of a RepA protein coding gene sequence in pVS1 of a non-T-DNA region is mutated into a histidine coding sequence from an arginine coding sequence. The invention aims to enhance efficient genetic transformation of plant exogenous genes in multiple species, has broad-spectrum adaptability to plant species, and particularly has obvious effects in multiple plant species which depend on genotypes and are difficult to transform, such as cucumbers, white radishes, broad beans, Chinese little greens, black nightshade, pokeberry roots, gingko, phalaenopsis, Chinese roses, Chinese pines and the like.

The invention provides an intelligent regulation and control method and system for a plateau afforestation habitat, and belongs to the technical field of ecological intelligent regulation and control. The method comprises the following steps: S1, acquiring multi-source data to construct a habitat database, and quantifying habitat demand parameters of target species; s2, calculating a comprehensive ecological function value and a species suitability index, and identifying a habitat demand conflict region; s3, designing a regulation and control scheme and pre-evaluating benefits; s4, outputting an optimal scheme through a multi-objective optimization algorithm by taking multi-species overall benefit maximization and balance degree optimization as objectives; s5, after the scheme is implemented, feedback data are obtained through a monitoring network, actual and expected benefits are compared, and when the deviation exceeds a threshold value, model parameters are corrected in a linkage mode, and closed-loop optimization is formed. According to the intelligent regulation and control method and system for the plateau afforestation habitat, habitat quality dynamic accurate diagnosis and simulation optimization decision making are achieved, a real-time feedback self-adaptive management mechanism is constructed, and the diversity of plateau animals and birds is improved.

The invention discloses an oligonucleotide pool for specifically digesting a virus transcript in pitaya and application of the oligonucleotide pool. Belongs to the technical field of biology. At present, a technology capable of specifically eliminating RNA of a specific species in mixed RNA of multiple species does not exist. According to the invention, transcripts of virus types in pitaya are assembled and analyzed, and a set of independent oligonucleotide pool with universality in pitaya is designed. By applying the oligonucleotide pool, RNase H and DNase I to pitaya mixed RNA, high-purity pitaya RNA can be obtained for next RNA sequencing, and the invention of the technology has important significance on molecular biology research of pitaya.

The invention discloses a formula of a broad-spectrum culture medium adapted to sterile sowing of multiple species of Chinese orchid, and relates to the technical field of plant tissue culture, the broad-spectrum culture medium adopts a 1 / 2MS basic culture medium, the dosage of a macroelement mother solution is halved, and the dosage of ferric salt, microelement and vitamin mother solution is kept unchanged; 10 mg of modified graphene, 1.5 mg to 2.5 mg of 6-benayl aminopurine (6-BA), 0.3 mg to 0.8 mg of naphthylacetic acid (NAA), 0.2 mg to 0.8 mg of zeatin (ZT), 0.05 mg to 0.2 mg of indolebutyric acid (IBA), 1.2 g to 2.2 g of lactoalbumin hydrolysate, 0.6 g to 1.0 g of activated carbon, 22 g to 32 g of saccharose and 5.5 g to 7.5 g of agar are added into every 1 L of the basic culture medium. The pH value of the culture medium is adjusted to 5.2-5.8. By adding the modified graphene into the culture medium, germination of Chinese orchid seeds is effectively promoted. Meanwhile, a complete nutrition system and a root system induction mechanism enhance the growth vigor of the seedlings and improve the survival rate of the seedlings, and meanwhile the universality of the culture medium is remarkably improved through the formula of the culture medium.

This invention relates to the field of electrical distribution cabinet technology, and provides a method, system, medium, and device for animal deterrence in electrical distribution cabinets. The method includes the following steps: acquiring image information of an animal located at a first preset distance; acquiring the animal's species information and pose information based on the image information; determining whether the animal is moving towards the electrical distribution cabinet based on the acquired pose information; if the animal is moving towards the cabinet, determining the animal's species information; the species information includes the number of species; if there are multiple species, determining whether a food chain relationship exists between the species; if a food chain relationship exists, using a corresponding deterrence method based on the food chain relationship. Using the method of this application, when there are multiple species, mutual deterrence between multiple species can be achieved by utilizing food chain relationships, improving deterrence efficiency.

The invention relates to the technical field of biochips, in particular to a sturgeon SNP (Single Nucleotide Polymorphism) liquid chip and application thereof. The invention provides a sturgeon SNP (Single Nucleotide Polymorphism) liquid chip based on targeted capture sequencing aiming at the current situation that sturgeon molecular markers are limited and independent research and development of sturgeon chips are lacked at present. The sturgeon SNP liquid phase chip is designed on the basis of high-depth sequencing data of multi-species populations, the coverage of SNP sites is comprehensive, and the amount of information is rich; a targeted capture sequencing technology is adopted, so that the detection efficiency is high, the accuracy is high, and flexible expansion and upgrading can be realized. The sturgeon SNP liquid phase chip disclosed by the invention can simultaneously meet various application requirements of sturgeon molecular identification, genome selection, functional gene research and the like. The invention fills the blank of independent research and development of sturgeon liquid phase chips, and has important scientific research value and industrial application prospect.

The invention relates to the technical field of sand stabilization, in particular to a sand land restoration method based on the configuration of multiple species such as a herba violae compound and shrub and grass, and the sand land restoration method comprises the following steps: S1, applying a water-retaining agent on the surface of a to-be-treated sand land; s2, a herba violae compound is sprayed, and formation of biological soil crust is promoted; s3, the surface of the sand land is covered with a sunshade net; wherein the herba lysimachiae compound is prepared from herba lysimachiae, plant seeds and a compound microorganism growth promoting agent; the compound microorganism growth promoting agent is prepared from trehalose, glycerol, monopotassium phosphate, ferrous sulfate heptahydrate, EDTA (Ethylene Diamine Tetraacetic Acid) disodium, sodium molybdate and glycine betaine. According to the method, a biological crust technology and multi-species vegetation configuration are combined, the sand surface can be rapidly fixed, wind erosion can be resisted, soil nutrients and biological activity can be gradually improved, forward succession of an ecological system is promoted, and the method has the advantages of being low in cost, environmentally friendly, continuous in ecological benefit and the like.

A species feature descriptor construction method based on functional genomics is beneficial to providing a new method for toxicity prediction and risk assessment modeling of chemicals to multiple species, and is characterized by comprising the following steps: step 1, collecting proteome data of a target species; 2, deploying a software tool for extracting the functional gene information of the species on a cloud server, and developing a command line tool for the software tool to realize gene annotation of multiple species and uploading and downloading of data; 3, importing the proteome data into the tool software for query, matching and annotation to obtain functional genome information of the target species; step 4, preprocessing and visualizing the annotated functional genome information; and step 5, constructing species feature descriptors of multiple species based on the functional genome information.

The application discloses a kind of fish fattening field suitable ecological flow and system of deducing method, including the following steps: constructing the index system of hydraulic habitat of fattening field for quantifying habitat patch spatial heterogeneity, the hydraulic habitat index value of different flow conditions is analyzed and calculated using two-dimensional hydrodynamic model in the study water area, the flow corresponding to the most suitable hydraulic habitat index value of the fish habitat studied is selected as the suitable ecological flow of fattening field.The present application not only considers the target organism but also considers other organisms in the river, and is more conducive to creating suitable flow conditions for multiple species coexistence, and has certain scientific significance and reference value for fish fattening field protection and ecological restoration.

The invention discloses a codon optimization method based on Transform and related equipment. The method comprises the following steps: acquiring a pairing data set of multiple species; based on the paired data set, screening target samples by using a codon adaptation index, and sorting to obtain a fine tuning set; performing pre-training on a pre-constructed host perception Transform model by using the pairing data set to obtain a pre-trained model; the input of the encoder comprises embedding of an amino acid sequence and a host identifier, and the input of the decoder comprises embedding of a real codon corresponding to a DNA sequence; dividing the fine tuning set according to a target host, and performing host fine tuning on the pre-training model to obtain a target model; and performing codon optimization by using the target model. According to the method, the global codon use rule is learned through multi-species data set pre-training, host identifier embedding and targeted fine tuning are combined, the limitation of a traditional method is effectively overcome, and the method can be widely applied to the technical field of biological information processing.

The invention discloses a cross-species RBP-RNA interaction prediction method and system and application, and relates to the technical field of RNA post-transcription regulation and deep learning. The method comprises the following steps: acquiring a to-be-predicted RNA primary sequence, and performing structure prediction on the RNA primary sequence to obtain a secondary structure sequence of RNA; constructing an interaction prediction model, and training the interaction prediction model by using a data set formed by CLIP data of multiple species; and predicting the secondary structure sequence of the RNA and the primary sequence of the RNA by using the trained interaction prediction model to obtain the binding condition of the RNA binding protein and the RNA. According to the method, a deep learning model is constructed by utilizing an RBP evolutionary conservative signal and a tag smoothing regularization strategy, so that accurate prediction of multi-species RBP-RNA interaction can be realized, the cross-species prediction capability can be effectively improved, and the limitation of a traditional method is made up.

The present application provides a method and device for determining an enrichment probe library, wherein the method includes: obtaining the genomic sequences corresponding to the microorganisms to be enriched; determining the gene fragments corresponding to the conserved regions in the genomic sequence of each microorganism to be enriched; for each gene fragment corresponding to the microorganism to be enriched, determining the base combination corresponding to the microorganism to be enriched through a sliding window; filtering all the base combinations of the microorganisms to be enriched according to preset conditions; determining the enrichment probe library according to the identification information corresponding to each microorganism to be enriched and the filtered base combinations. The present application generates an enrichment probe library by generating base combinations according to the genomic sequences of the microorganisms to be enriched, and filtering the base combinations corresponding to multiple microorganisms to be enriched, thereby solving the technical problem of designing an enrichment probe library for multiple species or multiple nucleic acid sequences, and producing the technical effect of enriching multiple species or multiple nucleic acid sequences and removing host gene interference.

In co-culture, the interactions between multiple species have been analyzed. Typically, microorganisms growing in co-culture can be mixed in the same growth medium, grow in the same medium but separated by a medium exchange membrane, or grow alone when exposed to products produced by other microorganisms. The study of such cultures surpasses the study of isolated cultures as it more accurately represents the real-world phenomena. A co-culture module having a plurality of sample volumes separated by a vertical semipermeable membrane is described herein. The co-culture module is typically manufacturable, and may be disposable or reusable.

The invention relates to a variety-specific SNP (Single Nucleotide Polymorphism) site screening method and application thereof, and belongs to the technical field of variety identification. According to the variety specific SNP site screening method and the variety identification strategy suitable for the multiple species, by combining specific SNP site screening and population genetic structure model analysis, variety specific sites can be rapidly and accurately screened, variety identification can be achieved, the accuracy and efficiency of variety identification are greatly improved, and the method is suitable for large-scale popularization and application. Therefore, technical support is provided for germplasm resource protection, breeding innovation and high-quality development of seed industry.

A clinical decision support system and method for identifying microorganisms in a sample by determining long read nucleic acid fragment sequence data (LRS data) derived from multiple species in the sample, performing a taxonomic classification of the LRS data, determining abundance levels of multiple reference genomes based on the taxonomic identifiers of the LRS data, aligning the LRS data with a subset of the reference genomes, and performing a coverage analysis to determine the identity of one or more microorganisms present in the sample based on the coverage analysis.

The application discloses a Brassica napus dominant male sterility gene Ms-cd1, a promoter sequence and application thereof in plant breeding, belongs to a plant male sterility material creating technology, and comprises the following steps: constructing a gene expression vector by using a Ms-cd1 promoter mutation sequence shown in Seq ID No. 2 and a Ms-cd1 gene coding region sequence shown in Seq ID No. 4, then performing agrobacterium-mediated transformation to be introduced into target plant material, and screening to obtain a transgenic strain; the Ms-cd1 promoter and gene sequence are disclosed for the first time, a recombination expression vector of the Ms-cd1 promoter and gene sequence is constructed, and a gene editing technology is used to create a sterile line suitable for multiple species, thereby assisting the breeding of Brassica napus and multiple main crops, and laying a foundation for promoting the wide application of the dominant male sterility gene Pmt::Ms-cd1 in breeding.

Methods and systems are disclosed for determining a set of target sequences for detecting polynucleic acid originating from a plurality of candidate species, including obtaining a genomic sequence of each candidate species; generating a set of k-mers for each candidate species; and incorporating iteratively k-mers into the set of target sequences, and calculating a molecular identification code for each of the plurality of candidate species, until all candidate species have a unique molecular identification code. Methods and system are also disclosed for analyzing in a sample comprising polynucleic acid originating from a plurality of candidate species, including detecting if each one of a set of target sequences is present in the polynucleic acid; generating a molecular identification code comprising binary digits, wherein each binary digit encodes whether a corresponding target sequence is present in the polynucleic acid; and comparing the molecular identification code with known molecular identification codes.

The invention discloses a comprehensive augmented culture sea area space division method based on machine learning, and relates to the technical field of comprehensive augmented culture sea area space division. The method comprises the following steps: for each augmented culture species, quantifying the influence weight of a plurality of environment variables on the distribution of the augmented culture species, and eliminating redundant environment variables in the plurality of environment variables according to the influence weight to obtain a target environment variable; inputting the target environment variable into the maximum entropy model, and respectively predicting suitable growth area distribution raster data of the proliferation and culture species; calculating a landscape pattern index of the suitable growth area, positioning a multi-species ecological niche overlapping area, and generating a conflict thermodynamic diagram based on the landscape pattern index and the multi-species ecological niche overlapping area; and in a geographic information system platform, stacking the suitable growth area distribution raster data of all the proliferation and culture species according to the weight, and determining a preferential culture area, a limited culture area and a prohibited culture area according to the stacking result and the conflict thermodynamic diagram. The method can improve the utilization rate of sea area resources.

The present invention relates to a plant efficient genetic transformation vector based on rhizobium rhizobium, which is modified based on the pTi vector, and its T-DNA left border pTi-LB and right border pTi-RB are replaced with the left border pRi-LB and the right border pRi-RB respectively; its T-DNA region is deleted. Hyg expression cassette, while in its original RUBY The front end of the expression cassette is connected to the target gene expression cassette; in its non-T-DNA region Ori Components and SmR Insert between components VirGN54D insert The present invention aims to enhance the efficient genetic transformation of exogenous plant genes in multiple species, with broad adaptability across a wide range of plant species, particularly in genotype-dependent and difficult-to-transform plant species such as cucumber, white radish, broad bean, cauliflower, nightshade, pokeweed, ginkgo, phalaenopsis, rose, and Chinese pine.

The application belongs to the technical field of genetic engineering, and particularly relates to a transport gene NtABCG6 gene and application thereof in promoting genetic transformation efficiency of multiple species. The nucleotide sequence of the NtABCG6 gene is shown as SEQ ID No. 1. By expressing the NtABCG6 gene, the transition of callus to bud differentiation can be significantly accelerated, and the period from agrobacterium infection to obtaining a rooting positive plant is shortened. Stable regeneration promotion effects are exhibited in Nicotiana tabacum, tomato and potato, and a new strategy is provided for constructing a high-efficiency, stable and low-intervention plant genetic transformation system.

The application discloses a crop mitochondrial editing site prediction method and system and a storage medium. Mitochondrial genome sequences of multiple species and corresponding C-to-U RNA editing site annotation information are obtained. Fragments of a set length are taken from the upstream and downstream of the mitochondrial genome sequence and the corresponding C-to-U RNA editing site annotation information sequence as positive samples with the editing site as the center. Any site without editing site annotation is taken as the center, and fragments of a set length are taken from the upstream and downstream of the mitochondrial genome sequence and the corresponding C-to-U RNA editing site annotation information as negative samples. Part of the positive samples and the negative samples corresponding to each species are used to construct a training set of each species, the training set of each species is used as the input of a convolutional neural network, and the optimal prediction model corresponding to each species is obtained. The application has high prediction accuracy and can better fit the potential encoding mechanism of C-to-U RNA editing of different species.