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169 results about "Multiple species" patented technology

Method and product for localized or spatial detection of nucleic acid in a tissue sample

The present invention relates to methods and products for the localized or spatial detection of nucleic acid in a tissue sample and in particular to a method for localized detection of nucleic acid in a tissue sample comprising: (a) providing an array comprising a substrate on which multiple species of capture probes are directly or indirectly immobilized such that each species occupies a distinct position on the array and is oriented to have a free 3′ end to enable said probe to function as a primer for a primer extension or ligation reaction, wherein each species of said capture probe comprises a nucleic acid molecule with 5′ to 3′: (i) a positional domain that corresponds to the position of the capture probe on the array, and (ii) a capture domain; (b) contacting said array with a tissue sample such that the position of a capture probe on the array may be correlated with a position in the tissue sample and allowing nucleic acid of the tissue sample to hybridize to the capture domain in said capture probes; (c) generating DNA molecules from the captured nucleic acid molecules using said capture probes as extension or ligation primers, wherein said extended or ligated DNA molecules are tagged by virtue of the positional domain; (d) optionally generating a complementary strand of said tagged DNA and/or optionally amplifying said tagged DNA; (e) releasing at least part of the tagged DNA molecules and/or their complements or amplicons from the surface of the array, wherein said part includes the positional domain or a complement thereof; and (f) directly or indirectly analyzing the sequence of the released DNA molecules.
Owner:10X GENOMICS SWEDEN AB

Intelligent production lines capable of producing standard PC components of multiple species and production process

The invention provides an intelligent production lines capable of producing standard PC components of multiple species and production process. Two intelligent production lines are arranged between anA shaft to a C shaft and between the C shaft to an E shaft respectively, are symmetric relative to the C shaft, and are installed between 1-28 axis of each span; each production line comprises a moldplacing device, a mesh bar processing device, a truss bar processing device, a material distribution machine, a turnover machine, a curing kiln, a stacker and a tipper. According to the production lines, three kinds of standard PC components of laminated slabs, superposed shear walls with no heat preserving function and superposed shear walls with a heat preserving function can be produced simultaneously by a single production line, and an information system is utilized to achieve whole process control of designing, producing and transporting to further reduce labor force. Only eighteen workers are needed to work on the intelligent production line in a working time period, the number of the workers is only one fourth of that of a traditional PC production line, current worker shortage is prevented, the labor intensity is reduced, the product quality and production efficiency are improved, and the production cost of enterprises is reduced.
Owner:美好建筑装配科技有限公司

Primers, probe composition and kit for rapid identification of nine animal origin ingredients in food or feed, detection method for identification of nine animal origin ingredients in food or feed and application of primers, probe composition, kit and detection method

The invention belongs to the technical field of molecular biology and provides primers, a probe composition and a kit for rapid identification of nine animal origin ingredients in food or feed, a detection method for identification of the nine animal origin ingredients in the food or feed and application of the primers, the probe composition, the kit and the detection method. Origin ingredients of the food or feed containing multiple species can be identified rapidly by the primers, the probe composition and the real-time fluorescent PCR (polymerase chain reaction) joint detection kit. The detection method includes: designing a universal primer by taking 16SrDNA as a target gene, and designing specific probes of nine species by designing to construct internal amplification control and designing the specific probes aiming at internal control sequences; performing PCR amplification by three PCR reaction systems; interpreting the origin ingredients of the nine species directly through corresponding fluorescent probe signals and Ct values. The detection method is low in cost, time saving and high in efficiency and can achieve identification of multiple species simultaneously.
Owner:BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI

Method for restoring degraded wetland vegetation by improved wetland soil seed banks

The invention discloses a method for restoring degraded wetland vegetation by improved wetland soil seed banks. The method comprises the following steps: 1) according to a principle of adjusting measures to local conditions, selecting a soil seed bank from natural wetland seed banks, wherein the seed density of the seed bank is not less than 5000 grains.m<-2>; 2) mixing soil seed banks with different sources, or adding naturally harvested seeds; 3) preparing a biological rhzomorph; 4) adding the biological rhzomorph obtained in the step 3) to obtain an improved soil seed bank; and 5) covering improved seed bank soil on a wetland with degraded vegetation by adopting an exotic soil method, thereby forming vegetation by natural germination. The method for restoring the degraded wetland by using the soil seed banks, disclosed by the invention, has the following advantages: 1) materials are easy to obtain, the cost is low, and the operation is simple and feasible; 2) a wetland succession law is followed, the degraded wetland vegetation is restored quickly and naturally, and the invasion of alien species is controlled; and 3) multiple species coexist, the structure is stable and can realize self-renewal, and ecological landscapes are rich.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY

Flowering peach grafting and seedling method

The invention discloses a flowering peach grafting and seedling method which comprises the steps of (1) mountain peach rootstock seedling cultivation, (2) scion preparation, (3) scion treatment, (4) scion grafting, and (5) post-grafting management. According to the invention, cultivated mountain peach rootstock seedlings are vigorous in growth, good in root development and high in drought resistance and cold resistance. In addition, the seedling method is simple, and the cost is low. A plurality of different kinds of flowering peach scions can be grafted on each mountain peach rootstock seedling, so that the single-plant grafting survival rate can be enabled to reach up to 99%. A cultivated grafted seedling has multiple species in one plant, so that better viewing values are brought when the cultivated grafted seedling is in blossom. Magnetic field treatment is carried out on the scions before grafting, division and differentiation of cells at a notch can be promoted, the wound healing ability is improved, the healing time is shortened, growth of the scions can also be promoted, and the grafting survival rate is improved. The method disclosed by the invention can complete grafting of flowering peach in the same year when the mountain peach rootstock seedling is cultivated, thereby greatly shortening the seedling time, and improving the efficiency and the market competitiveness.
Owner:阜南县田隆花木种植有限公司
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