7 results about "Multiple species" patented technology

This invention discloses a genetic transformation and gene editing method applicable to multiple sesquiterpene species, belonging to the field of plant genetic transformation and gene editing technology. The method uses the cotyledonary nodes of sterile seedlings of the sesquiterpene genus as explants, and introduces an optimized pScEF1α-Cas9 gene editing transformation vector using Agrobacterium infection. Regenerated plants are obtained through recovery culture, selection culture, and rooting culture. The vector contains four gene expression units: pScEF1α:Cas9, pGmU6:sgRNA, pGmUBI:DsRed2, and pCaMV35S:HygR. In vivo screening is further performed using the DsRed2 fluorescent reporter gene, and the gene-edited plants are identified by molecular detection. This method is applicable to common sesquiterpene, stem-nodled sesquiterpene, and spiny sesquiterpene, and has advantages such as strong species versatility, high transformation efficiency, short cycle, visualized screening, and high positive rate, providing efficient technical support for gene function research and genetic improvement of sesquiterpene species.

The application discloses a construction method of a multiplex PCR reaction system for detecting and identifying Podosphaera species and application thereof. The method comprises the following steps: obtaining whole genome sequences of multiple Podosphaera species, and performing multiple sequence alignment to obtain specific genes and specific gene clusters of each species; taking the genes in the specific gene clusters of each species as templates, designing multiple upstream primers and downstream primers according to a conventional primer design method to generate primer pairs, and excluding unreasonable primer pairs and primer pairs with low sensitivity and specificity; according to the size of the amplification products, the primers are freely combined and matched to generate four pairs of mixed multiplex primers, and the multiplex PCR reaction conditions are optimized to obtain a multiplex PCR system capable of specifically detecting Podosphaera and simultaneously identifying single and multiple species. The multiplex PCR method for detecting and identifying Podosphaera provided by the application can quickly and accurately complete the detection of Podosphaera and identify the species identity of Podosphaera.

This invention relates to the field of electrical distribution cabinet technology, and provides a method, system, medium, and device for animal deterrence in electrical distribution cabinets. The method includes the following steps: acquiring image information of an animal located at a first preset distance; acquiring the animal's species information and pose information based on the image information; determining whether the animal is moving towards the electrical distribution cabinet based on the acquired pose information; if the animal is moving towards the cabinet, determining the animal's species information; the species information includes the number of species; if there are multiple species, determining whether a food chain relationship exists between the species; if a food chain relationship exists, using a corresponding deterrence method based on the food chain relationship. Using the method of this application, when there are multiple species, mutual deterrence between multiple species can be achieved by utilizing food chain relationships, improving deterrence efficiency.

The application discloses a Brassica napus dominant male sterility gene Ms-cd1, a promoter sequence and application thereof in plant breeding, belongs to a plant male sterility material creating technology, and comprises the following steps: constructing a gene expression vector by using a Ms-cd1 promoter mutation sequence shown in Seq ID No. 2 and a Ms-cd1 gene coding region sequence shown in Seq ID No. 4, then performing agrobacterium-mediated transformation to be introduced into target plant material, and screening to obtain a transgenic strain; the Ms-cd1 promoter and gene sequence are disclosed for the first time, a recombination expression vector of the Ms-cd1 promoter and gene sequence is constructed, and a gene editing technology is used to create a sterile line suitable for multiple species, thereby assisting the breeding of Brassica napus and multiple main crops, and laying a foundation for promoting the wide application of the dominant male sterility gene Pmt::Ms-cd1 in breeding.

The application belongs to the technical field of genetic engineering, and particularly relates to a transport gene NtABCG6 gene and application thereof in promoting genetic transformation efficiency of multiple species. The nucleotide sequence of the NtABCG6 gene is shown as SEQ ID No. 1. By expressing the NtABCG6 gene, the transition of callus to bud differentiation can be significantly accelerated, and the period from agrobacterium infection to obtaining a rooting positive plant is shortened. Stable regeneration promotion effects are exhibited in Nicotiana tabacum, tomato and potato, and a new strategy is provided for constructing a high-efficiency, stable and low-intervention plant genetic transformation system.

This invention provides a method for breeding and hatching fish fry, comprising the following steps: placing multiple fish ovaries sequentially into a sunflower seed coat, and adjusting the hatching mechanism to lower the ovaries below the water surface of the hatching tank; activating the heating component to heat and regulate the water temperature in the hatching tank, while simultaneously adjusting the water level of the ovaries within the tank; and continuously supplying oxygen to the hatching tank via an aeration mechanism, waiting for the fish fry in the ovaries to hatch. This invention, through the design of the hatching device, allows for the overall raising and lowering of the fish ovaries and independent, staged adjustments for each group. It enables precise position control for different fish species and different hatching cycles. One device can simultaneously hatch multiple species of fish fry, improving space and equipment utilization. Furthermore, after hatching, the fish fry can be collected centrally via a collection mechanism, avoiding mechanical damage caused by manual harvesting. The method is simple to operate, highly efficient, and suitable for large-scale aquaculture.

The present application relates to the technical field of molecular biology detection, and particularly relates to a primer probe combination product and kit for detecting Brucella. The present application realizes rapid determination of Brucella and accurate typing identification of B. abortus, B. melitensis and B. suis in the same detection process by constructing a multiplex primer probe combination containing genus level conservative targets and multiple species specific targets. The scheme reduces the operation complexity and time cost caused by traditional step-by-step detection, and improves the stability and reliability of the typing results through multi-target collaborative detection, and reduces the risk of misjudgment caused by single site variation. In addition, the preferred two-tube system and multi-channel detection mode are conducive to reducing the opening operation, reducing the cross contamination probability, and facilitating the standardization detection in different types of samples, and are conducive to improving the detection efficiency and result consistency.