3 results about "Nuclear membrane" patented technology

This invention discloses a method for constructing a tomato core membrane-associated protein interaction library based on Gateway technology under Fusarium oxysporum infection conditions and its application, relating to the field of genetic engineering technology. The method includes: using tomato root tissue infected with Fusarium oxysporum as material, extracting total RNA and enriching mRNA; synthesizing first-strand cDNA using Biotin-attB2-Oligo(dT) primers; synthesizing double-stranded cDNA enzymatically; ligating it with a specific double-stranded attB1 adapter; introducing attB1 and attB2 sites at both ends of the cDNA; cloning the cDNA into the donor vector pDONR222 via BP recombination to construct a primary entry library; and recombinating the primary entry library with vectors pGADT7 and pPR3-N via LR recombination to construct a nuclear secondary library and a membrane secondary library, respectively. The constructed libraries have high titers, long insert fragments, and low empty vector rates. This invention also relates to the application of the library in screening the interaction between Fusarium oxysporum effector proteins and tomato nuclear membrane-associated proteins, providing an efficient and specific resource platform for studying plant-pathogen interaction mechanisms.

The present application relates to the technical field of digital pathology image analysis, and discloses an animal tissue pathological section cell atypia image automatic grading method and system, the method comprises the following steps: scanning the whole section image to obtain a high-power field image by traversing the tumor area with a sliding window; inputting a star-convex polygon regression kernel instance segmentation network trained by multi-species animal tumor annotation data to obtain each nuclear boundary; extracting morphological parameters such as nuclear area, long-short axis ratio, nuclear-plasma ratio, nuclear membrane regularity and chromatin texture; detecting and counting nuclear mitotic figures; introducing a species adaptive baseline correction to normalize the morphological parameters and then inputting the normalized morphological parameters into a classifier to output a three-level atypia grading.

This invention discloses a method for constructing a cell nucleus model based on Abaqus-Python, relating to the field of cell stress analysis technology. This method utilizes Abaqus finite element software to draw a cell nucleus model and analyze its mechanical properties. Simultaneously, it employs Python for secondary development, simplifying the model drawing process through scripting and improving the efficiency and accuracy of model creation. This model can be used to calculate the stress distribution of the cell nucleus's main structures—the nuclear membrane and nuclear lamina—during the process of nanoneedles penetrating the cell nucleus, and to optimize nanoneedle parameters by analyzing the stress concentration of the nuclear membrane during penetration.