100 results about "Nuclear membrane" patented technology

The present invention provides a method for allowing foreign particles to penetrate, very efficiently, the cell wall, cell membrane, organelle membrane and / or nuclear membrane of a cell and hybridizing or binding to the complimentary target in the cell. The cells may be from a culture or from specimens obtained from a patient. The foreign particle can be a probe consisting of, for example, either individually or in any combination of two or more of the following: DNA, RNA, peptide nucleic acids (PNA), glycopeptides, lipopeptides, glycolipids or prions. The target is a cell, a cell component or, preferably, a pathogen or pathogen component. The pathogen can be, for example, bacteria, fungi, yeast or viruses.

The present invention provides a method for allowing foreign particles to penetrate, very efficiently, the cell wall, cell membrane, organelle membrane and / or nuclear membrane of a cell and hybridizing or binding to the complimentary target in the cell. The cells may be from a culture or from specimens obtained from a patient. The foreign particle can be a probe consisting of, for example, either individually or in any combination of two or more of the following: DNA, RNA, peptide nucleic acids (PNA), glycopeptides, lipopeptides, glycolipids or prions. The target is a cell, a cell component or, preferably, a pathogen or pathogen component. The pathogen can be, for example, bacteria, fungi, yeast or viruses.

Provided is a method of treating or preventing a cardiomyopathy associated with activation of at least one kinase in the MAP kinase signaling pathway in heart tissue by providing to a subject an inhibitor of at least one kinase in the ERK signaling pathway or in the JNK signaling pathway, or both. In some embodiments, the cardiomyopathy is associated with one or more mutations in the LMNA gene, which encodes A-type nuclear lamins, or in the EMD gene, which encodes an inner nuclear membrane protein.

The invention relates to a novel coal dust suppressant with a nuclear membrane structure and a preparation method thereof. The novel coal dust suppressant comprises the following components in percentage by weight: 1.0-2.0% of modified cellulose or modified starch, 0.1-0.5% of modified nanoparticles and 97.5-98.9% of water. The dust suppressant of the invention can be proportioned in a normal temperature state, and other additional heating devices are not needed. The dust suppressant of the invention can be sprayed or coated on the surface of coal in a carriage after being proportioned at normal temperature, so that a curing layer is formed on the surface of the coal, thereby avoiding dust pollution of the coal on railway lines in the railway transport process and avoiding dust loss of the coal; and environment pollution can not be caused. The invention not only has great economic benefit for railway transport and coal customers but also has social benefit of environment protection.

The invention provides a mercury-free environment-friendly hematoxylin staining solution, which comprises the following components in portions by volume: 100-300 portions of 3-10 percent hematoxylin absolute ethyl alcohol solution, 1500-2000 portions of 2-6 percent aluminum sulfate aqueous solution, 60-100 portions of .05-2 percent periodic acid aqueous solution, and 15-30 portions of glacial acetic acid. Non-toxic and innocuous chemical reagents are adopted for the mercury-free environment-friendly hematoxylin staining solution, and a histological section staining experiment shows that the nucleus is clearly stained and the structures of the chromatin, nuclear membrane and nucleolus are clear.

The present invention includes compositions, methods and kits for directing an agent across the nuclear membrane of a cell. The present invention includes a Karyopherin beta2 translocation motif in a polypeptide having a slightly positively charged region or a slightly hydrophobic region and one or more R / K / H-X(2-5)-P-Y motifs. The polypeptide targets the agent into the cell nucleus.

The invention discloses a tumor cell membrane / nuclear membrane double-targeting tumor nano-drug slow-release system and preparation and application thereof. The tumor cell membrane / nuclear membrane double-targeting tumor nano-drug slow-release system is a nano particle composed of a nucleus and a shell, wherein the shell is composed of an aptamer, PEG and nano liposomes, and the aptamer is connected with the nano liposome through the PEG and capable of specifically recognizing a tumor cell; the nucleus is composed of a nano material modified by polypeptide and an antineoplastic drug carried by the nano material modified by the polypeptide, and the polypeptide is capable of penetrating through a cell nucleus nuclear membrane. It is proved through experiments that the tumor cell membrane / nuclear membrane double-targeting tumor nano-drug slow-release system can inhibit tumor growth and prolong the survival time of a tumor-bearing animal and can be used for treating a tumor.

The invention provides red fluorescent carbon quantum dots used for detecting pH in cells and a preparation method and application thereof. The preparation method comprises the following steps: (1) weighing 0.1-0.2mmol of p-phenylenediamine solution and dissolving in 0.075-0.15mol of ethylenediamine, to obtain faint yellow liquid; (2) arranging the solution to a microwave oven, reacting for 5-25min with the power of 20-100%, cooling to room temperature after reaction is ended, and adding 5mol / L of HCL until gas escapes; and (3) putting the obtained solution into a dialysis bag of 500-1000Da, then putting the dialysis bag into a containing with water for dialysis for two days, to obtain a pure carbon quantum dot water solution, and performing freeze drying on the obtained pure carbon quantum dot water solution to obtain light pink flocculent carbon quantum dots. The carbon quantum dots prepared according to the invention have the characteristics of long launching, high quantum yield, good biocompatibility and the like; and the carbon quantum dots have sensitive response to pH and can penetrate a nuclear membrane to enter a cell nucleus, so as to enter the whole cell, thus achievingan effect of detecting the pH of the whole cell.

The invention relates to a preparation method of a coal dust suppressant with a nuclear membrane structure, comprising the following ingredients in parts by weight: 1.0-2.0 parts of modified cellulose or modified starch, 0.3-3.0 parts of nano particle matrix, 4.0-8.0 parts of alcohol and 87-94.7 parts of water. The nano particle matrix is dissolved in the alcohol; the modified starch or the modified cellulose is dissolved in the water; the two mixtures are evenly mixed to ensure that the pH value of mixed liquid is 6.5-7.5; and then, the mixed liquid is stirred for 5-8 hours. The dust suppressant of the invention capable of preventing coal dust emission can be proportioned and prepared at normal temperature without adding other warming-up devices. After being proportioned at normal temperature, the coal dust suppressant is sprayed or coated on the surface of compartment coal to ensure that a solidified layer is formed on the surface of the coal so as to avoid dust raising pollution and coal dust raising loss along the line in the railway transportation process and can not cause environment pollution. In addition, the invention not only has great economic benefit for railway transportation and coal clients but also has social benefits for environment protection.

A vibration type microinjection device capable of ensuring smooth piercing of membranes having different properties such as a zona pellucida, a cell membrane and a nuclear membrane included in a fertilized egg with high accuracy and efficiency is provided.A vibration type microinjection device comprises a vibrator (28) which is connected in series with a micropipette (8) and which has a piezoelectric actuator (29) installed in a housing, and a signal control device (21) for controlling an electric signal applied to the piezoelectric actuator (29), wherein vibration is applied in the longitudinal direction of the micropipette (8) via the vibrator (28) by inputting an electric signal to the piezoelectric actuator (29). By such configuration, smooth piercing of membranes having different properties such as a zona pellucida, a cell membrane and nuclear membrane included in a fertilized egg is realized with high accuracy and efficiency.

Systems and methods for targeting specific cell types by selective application of ultrasonic harmonic excitation at their resonance frequency (“oncotripsy”) are presented. The systems and methods result in the permeabilization, lysis, and / or death of targeted cell types by using ultrasonic harmonic excitations that have their frequency and pulse duration specifically tuned to disrupt the nuclear membrane of the targeted cells types by inducing a destructive vibrational response therein while leaving non-targeted cell types intact. Target cells may be neoplastic.

The invention belongs to the field of medicament and pharmaceutics, and relates to a magnetic compound. Specifically, the magnetic compound has a nuclear-casing structure, which means that an external layer is a segmented copolymer of chemical bond gathered basic amino acids or derivatives thereof, and a nuclear is metal oxide particles. A first block of the segmented copolymer is polyethylene glycol, and a second block is polyacrylic acid monoglyceride or polymethacrylic acid monoglyceride, wherein the second block is combined with surfaces of Fe3O4 particles. The invention also relates to apreparation method and a purpose of the magnetic compound. The magnetic compound of the invention not only has low toxicity, but also can permeate a cell membrane or even a karyotheca to enter a nucleus, or even pass through a blood cerebral barrier (BBB), so the magnetic compound can be used as a conveying carrier of various nucleic acids / medicaments.

A modified virus including one or more non-native polypeptides, and the use of such viruses in therapy, particularly in the treatment of tumours or other cancerous cells are disclosed. The polypeptides includes one or more framework moieties each containing one or more binding moieties, and is capable of being expressed in the cytoplasm and nucleus of a mammalian host cell in a conformation which is maintained in the absence of a ligand for the binding moieties. The conformation allows the binding moieties subsequently to bind with the ligand, and the polypeptide is capable of transport through the nuclear membrane, wherein the modified virus has an altered tropism conferred by the binding moieties.

Provided herein are methods of recovering single nuclei from a tissue sample comprising chopping or dounce homogenizing the tissue sample in a nuclear extraction buffer at 4° C. to produce a tissue homogenate; centrifuging the tissue homogenate to produce a nuclear pellet; resuspending the nuclear pellet in a nuclear resuspension buffer comprising bovine serum albumin, RNase inhibitor, and salts to produce a resuspension; and filtering the resuspension through a strainer, wherein the single nuclei are present in a supernatant passed through the strainer. The invention also provides a method of single cell sequencing comprising extracting nuclei from a population of cells under conditions that preserve a portion of the outer nuclear envelope and rough endoplasmic reticulum; sorting single nuclei into separate reaction vessels; extracting RNA from the single nuclei; generating a cDNA library, whereby gene expression data from single cells are obtained. The tissue sample may be fresh or frozen.

Inositol derivatives in accordance with the present invention are effective in significantly enhancing the transportation of various therapeutic molecules across a biological membrane, which may include the plasma membrane, nuclear membrane or blood-brain barrier.

Disclosed herein is a nanoconstruct comprising an aptamer and a gold nanostar. The nanoconstruct can be used in a method of inducing changes to a nuclear phenotype of a cell comprising transporting the nanoconstruct to a nucleus of a cell, and releasing the aptamer from a surface of the gold nanostar into the nucleus of the cell to afford deformations or invaginations in the nuclear membrane, thereby inducing changes to the nuclear phenotype. The method can be used to treat certain hyperproliferative disorders such as cancer.

The present invention relates to nucleic acid target-specific chimeric proteins comprising a nuclear envelope and / or lamina binding domain and a DNA binding domain. These proteins, as well as nucleic acids encoding these proteins, can be used in methods for suppressing or downregulating the expression of selected genes. The DNA binding domain is preferably derived from a naturally occurring zinc finger protein (ZFP) or an artificial zinc finger protein (AZP). The present invention also provides molecular switch systems for gene regulation.

Although it can be farnesylated, the mutant lamin A protein expressed in Hutchison Gilford Progeria Syndrome (HGPS) cannot be defarnesylated because the characteristic mutation causes deletion of a cleavage site necessary for binding the protease ZMPSTE24 and effecting defarnesylation. The result is an aberrant farnesylated protein (called “progerin”) that alters normal lamin A function as a dominant negative, as well as assuming its own aberrant function through its association with the nuclear membrane. The retention of farnesylation, and potentially other abnormal properties of progerin and other abnormal lamin gene protein products, produces disease. Farnesyltransferase inhibitors (FTIs) (both direct effectors and indirect inhibitors) will inhibit the formation of progerin, cause a decrease in lamin A protein, and / or an increase prelamin A protein. Decreasing the amount of aberrant protein improves cellular effects caused by and progerin expression. Similarly, treatment with FTIs should improve disease status in progeria and other laminopathies. In addition, elements of atherosclerosis and aging in non-laminopathy individuals will improve after treatment with farnesyltransferase inhibitors.

The invention discloses a super-resolution optical imaging probe for living cells and a preparation method of the super-resolution optical imaging probe. Quantum dots are taken as a fluorophore, cell membrane penetrating peptides and nucleic acid aptamers are coupled on the surfaces of the quantum dots, the cell membrane penetrating peptides are used for promoting the probe to penetrate through the living cells, the nucleic acid aptamers are used for specific binding with nuclear membrane receptor protein of the living cells, and cell nucleus positioning of the probe is realized. The quantum dots with scintillating effects are taken as the fluorophore and are adapted to super-resolution optical imaging based on a single-molecule positioning method by means of the high luminous intensity and the fluorescence scintillation characteristic; meanwhile, the quantum dots with scintillating effects are taken as the fluorophore, a special imaging buffering solution is not required, therefore, the probe is adapted to super-resolution imaging of the living cells; besides, the preparation method is simple and convenient to operate, the cost is low, and the time cost and the economic cost are effectively saved.

A method of creating a model of a biological cell. The method features providing a circular base with indentations disposed therein, forming a cell membrane and at least one organelle from a mixture formed by mixing all-purpose flour and water. Organelles may include a nuclear membrane, a cell wall, mitochondria, a smooth endoplasmic reticulum (ER), a rough ER, a vacuole, a golgi apparatus, a nucleolus, a nucleoplasm, chromatin, a ribosome, DNA, RNA, transcription machinery, a histone, and a microtubule. The outside edge of the base is lined with the cell membrane. The organelles are inserted into indentations or atop the base. The organelles can be secured with glue.

Systems and methods for targeting specific cell types by selective application of ultrasonic harmonic excitation at their resonance frequency (“oncotripsy”) are presented. The systems and methods result in the lysis of targeted cell types by using ultrasonic harmonic excitations that have been specifically tuned to disrupt the nuclear membrane of the targeted cells types by inducing a destructive vibrational response therein while leaving non-targeted cell types intact, The target cells types may be cancerous cells.

Disclosed are novel cell-penetrating transporters for enhancing cellular uptake of peptide fusion proteins into live cells. The cell-penetrating transporters comprise a functionalized peptide construct comprising a cell importation peptide covalently bound to a cargo, the cell importation peptide in an unreactive monomeric form, and a pharmaceutical carrier. In certain embodiments, the cargo further comprises a nuclear localization sequence (NLS) allowing the cargo to be transported across the nuclear membrane.

The invention provides a PLK1 inhibitor, and a preparation method and an application thereof. The PLK1 inhibitor is a compound represented by general formula (I) shown in the description, and stereoisomers or pharmaceutically acceptable salts thereof. All groups in the formula (I) are defined as in the description. The PLK1 inhibitor can specifically bind to a DNA sequence in a PLK1 gene transcription promoter region, represented by SEQ: NO:1, in a high strength manner, inhibits transcription of the PLK1 gene, inhibits the expression of a PLK1 protein, causes tumor cell growth inhibition or apoptosis, can traverse through a cell membrane and a nuclear membrane and resist nuclease hydrolysis, and also solves the drug resistance problem of micro-molecular kinases inhibitors.

Although it can be farnesylated, the mutant lamin A protein expressed in Hutchinson Gilford Progeria Syndrome (HGPS) cannot be defarnesylated because the characteristic mutation causes deletion of a cleavage site necessary for binding the protease ZMPSTE24 and effecting defarnesylation. The result is an aberrant farnesylated protein (called “progerin”) that alters normal lamin A function as a dominant negative, as well as assuming its own aberrant function through its association with the nuclear membrane. The retention of farnesylation, and potentially other abnormal properties of progerin and other abnormal lamin gene protein products, produces disease. Farnesyltransferase inhibitors (FTIs) (both direct effectors and indirect inhibitors) will inhibit the formation of progerin, cause a decrease in lamin A protein, and / or an increase prelamin A protein. Decreasing the amount of aberrant protein improves cellular effects caused by and progerin expression. Similarly, treatment with FTIs should improve disease status in progeria and other laminopathies. In addition, elements of atherosclerosis and aging in non-laminopathy individuals will improve after treatment with farnesyltransferase inhibitors.

The invention discloses a method for obtaining an action mechanism of a nanometer probe and a single cell, which relates to the fields of atomic force microscope and cytomechanics. A cell membrane piercing force (F1) and a cell nuclear envelope piercing force (F2) are determined to being able to reflecting external forces that cells can bear by curves and thus the external force values that are needed at least for piercing the cell membrane and the cell nuclear envelope can be reflected in a quantitative mode. The piercing force hopping (Fd1,k1) of the cell membrane and the hopping (Fd2,k2) of the cell nuclear envelope piercing force can reflect differences between external and internal materials of the cell membrane and external and internal materials of the cell nuclear envelope; the larger the Fd and k values, the larger the characteristic differences of the external and internal materials of the cell membrane and cell nuclear envelope; and when the differences are larger than certain values, piercing of the needle point into the cell membrane or the cell nuclear envelope can be determined. On the basis of statistics of lots of data, the Fd is set to be larger than 0.07Nn and the k is set to be larger than 0.5Nn / microns; and because of the quantitative statistics, the determination data can be used as piercing data for determining a cell membrane and a cell nuclear envelope intelligently in future.

The inventive molecular transporter compound shows significantly high permeability through a biological membrane such as a plasma membrane, nuclear membrane and blood-brain barrier, and accordingly, can be effectively used in delivering various biologically active molecules.

Improved systems and methods for the analysis of digital images are provided. More particularly, the present disclosure provides for improved systems and methods for the analysis of digital images of biological tissue samples. Exemplary embodiments provide for: i) segmenting, ii) grouping, and iii) quantifying molecular protein profiles of individual cells in terms of sub cellular compartments (nuclei, membrane, and cytoplasm). The systems and methods of the present disclosure advantageously perform tissue segmentation at the sub-cellular level to facilitate analyzing, grouping and quantifying protein expression profiles of tissue in tissue sections globally and / or locally. Performing local-global tissue analysis and protein quantification advantageously enables correlation of spatial and molecular configuration of cells with molecular information of different types of cancer.