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Red fluorescent carbon quantum dots used for detecting pH in cells and preparation method thereof

A carbon quantum dot and red fluorescence technology, applied in the field of red fluorescent carbon quantum dots and their preparation, can solve the problems of limitation, limited application, inability of nuclear membrane to enter the nucleus, etc., and achieve high quantum yield, good biocompatibility, Responsive effect

Active Publication Date: 2018-04-17
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, synthesized carbon quantum dots usually emit blue and green fluorescence, which limits their application in the biological field
In addition, in terms of cell imaging, most of the current fluorescent nanoprobes are limited to the cell matrix and cannot penetrate the nuclear membrane into the nucleus.

Method used

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  • Red fluorescent carbon quantum dots used for detecting pH in cells and preparation method thereof
  • Red fluorescent carbon quantum dots used for detecting pH in cells and preparation method thereof
  • Red fluorescent carbon quantum dots used for detecting pH in cells and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A preparation of red fluorescent carbon quantum dots for detecting intracellular pH in this embodiment comprises the following steps:

[0021] (1) Weigh 0.2 mmol of p-phenylenediamine and dissolve it in 0.15 mol of ethylenediamine to obtain a light yellow liquid;

[0022] (2) Place the above solution in a microwave oven, react at 20% power for 20 minutes, cool to room temperature after the reaction stops, add 5mol / L HCl until gas escapes;

[0023] (3) Put the above obtained solution into a dialysis bag of 500-1000Da, then put the dialysis bag into a 1000ml beaker, and dialyze for two days to obtain a pure aqueous solution of carbon quantum dots, which is freeze-dried to obtain light pink flocs carbon quantum dots. The quantum yield of the carbon quantum dots was measured to be 14%.

[0024] Dissolve the obtained carbon quantum dots in PBS buffer solutions with pH=5.8 and pH=7.4 respectively, make 50μg / ml solutions, add them to the culture medium of A549 cells, incubat...

Embodiment 2

[0030] (1) Weigh 0.1 mmol of p-phenylenediamine and dissolve it in 0.15 mol of ethylenediamine to obtain a light yellow liquid;

[0031] (2) Place the above solution in a microwave oven, react at 40% power for 20 minutes, cool to room temperature after the reaction stops, add 5mol / L HCl until gas escapes;

[0032] (3) Put the above obtained solution into a dialysis bag of 500-1000Da, then put the dialysis bag into a 1000ml beaker, and dialyze for two days to obtain a pure aqueous solution of carbon quantum dots, which is freeze-dried to obtain light pink flocs carbon quantum dots. The quantum yield of the carbon quantum dots was measured to be 12%.

Embodiment 3

[0034] (1) Weigh 0.2 mmol of p-phenylenediamine and dissolve it in 0.075 mol of ethylenediamine to obtain a light yellow liquid;

[0035] (2) Place the above solution in a microwave oven, react at 60% power for 15 minutes, cool to room temperature after the reaction stops, add 5mol / L HCl until gas escapes;

[0036] (3) Put the above obtained solution into a dialysis bag of 500-1000Da, then put the dialysis bag into a 1000ml beaker, and dialyze for two days to obtain a pure aqueous solution of carbon quantum dots, which is freeze-dried to obtain light pink flocs carbon quantum dots. The quantum yield of the carbon quantum dots was measured to be 10%.

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Abstract

The invention provides red fluorescent carbon quantum dots used for detecting pH in cells and a preparation method and application thereof. The preparation method comprises the following steps: (1) weighing 0.1-0.2mmol of p-phenylenediamine solution and dissolving in 0.075-0.15mol of ethylenediamine, to obtain faint yellow liquid; (2) arranging the solution to a microwave oven, reacting for 5-25min with the power of 20-100%, cooling to room temperature after reaction is ended, and adding 5mol / L of HCL until gas escapes; and (3) putting the obtained solution into a dialysis bag of 500-1000Da, then putting the dialysis bag into a containing with water for dialysis for two days, to obtain a pure carbon quantum dot water solution, and performing freeze drying on the obtained pure carbon quantum dot water solution to obtain light pink flocculent carbon quantum dots. The carbon quantum dots prepared according to the invention have the characteristics of long launching, high quantum yield, good biocompatibility and the like; and the carbon quantum dots have sensitive response to pH and can penetrate a nuclear membrane to enter a cell nucleus, so as to enter the whole cell, thus achievingan effect of detecting the pH of the whole cell.

Description

technical field [0001] The invention relates to nanometer materials, in particular to a red fluorescent carbon quantum dot for detecting the pH in the whole cell, a preparation method and application thereof. Background technique [0002] As an important metabolic and intracellular parameter, cellular pH plays a key role in regulating the physiological and pathological processes of many cells. Abnormal pH will cause dysfunction of cells and tissues in the body, inhibit the activity of enzymes and proteins, reduce the body's immunity, and eventually lead to diseases such as inflammation, cancer, and Alzheimer's disease. In addition, changes in cellular pH are closely related to apoptosis. In the initial stage of apoptosis, cancer cells generally cause intracellular acidification, and intracellular acidification has become an important early feature of apoptosis. Therefore, sensitive and accurate real-time in situ monitoring of cellular pH is helpful for understanding cellul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65G01N21/64B82Y20/00B82Y40/00
CPCB82Y20/00B82Y40/00C09K11/65G01N21/6428
Inventor 丁媛媛弓晓娟焦媛高艺芳路雯婧双少敏董川
Owner SHANXI UNIV
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