13 results about "Intracellular pH" patented technology

The application provides a near-infrared fluorescent pH probe and a preparation method and application thereof, and belongs to the technical field of biosensing; the chemical structural formula of the probe is as follows: the novel near-infrared fluorescent pH probe can detect in a more acidic range, for example, when the pH changes from 6.40 to 3.00, the fluorescence intensity at 790 nm is enhanced by nearly 70 times, and the fluorescent effect is better; the fluorescent pH probe has low cytotoxicity, high stability and good biocompatibility; and can effectively perform fluorescence imaging on different intracellular pH.

The invention relates to a new chemogenetic tool manipulating intracellular pH levels and hydrogen sulfide levels in living cells and tissues with high spatio-temporal resolution.

This invention provides methods to determine the stage in the natural time course of immune response in a subject. It describes method to control immune response by targeting regulatory mechanisms differentially operating in lymphocytes at different stages in the natural time course of immune response. One kind of such regulatory mechanism is the mechanisms of energy metabolism that control intracellular pH (pHi), which in turn control cell death and proliferation. This invention also describes a combinatorial approach to lowering pHi of neoplastic cells to induce their death using compositions of combinations of regulators of energy metabolism. Finally, this invention provides methods to treat skin or mucosa infections or neoplasia by topical application of pH modifier(s) that lower pH.

Compositions and methods are disclosed herein for inducing (up-regulating) the expression of sulfotransferases in the hair follicles, e.g., the scalp. Increasing sulfotransferase is beneficial for metabolizing pro-drugs that require sulfonation to be activated, e.g., minoxidil sulfate is the active metabolite of minoxidil. A method for combining the compositions described herein with topical minoxidil to enhance minoxidil treatment for androgenetic alopecia is described. Additional methods and compositions include the use of retinoid X receptor agonists, retinoic acid receptor agonists, and nuclear receptor agonists in an RXR-NR heterodimer. Additional methods and compositions include the use of a topical solution containing an alkalinizing agent or an alkalinizing agent used with a penetration enhancer for up-regulating the sulfonating capacity of hair bearing skin, hair follicles, and / or keratinocyte cells. In addition, compositions and methods for increasing or decreasing the growth rate of hair follicles is disclosed by altering the intracellular pH. Additionally, methods for preparing a stable liposomal containing solution are described.

The invention relates to a new chemogenetic tool manipulating intracellular pH levels and hydrogen sulfide levels in living cells and tissues with high spatio-temporal resolution.

The application provides an organic room-temperature phosphorescent molecular compound and a preparation method and application thereof. The organic room-temperature phosphorescent molecular compound provided in the application takes a six-substituted arene phosphorescent core as a core molecule, and a group capable of inducing intermolecular hydrogen bonding is modified on the core molecule to form different aggregation states through pi-pi stacking and hydrogen bonding. The preparation method of the compound comprises the following steps: taking a six-substituted benzene as a basis, further synthesizing a six-substituted arene structure as a compound skeleton, and then introducing groups including a carboxyl group, a carboxyl group derivative, a hydroxyl group or an amide on the skeleton. The organic room-temperature phosphorescent molecular compound can be used for cell life imaging and as a pH sensing probe for detecting intracellular pH changes. The compound provided in the application has a life of up to 30 mu s when the water content is 99%, can respond to pH, and provides accurate and reliable life biological imaging in clinical biomedical applications; and when used as a pH sensing probe, the compound can accurately respond to intracellular pH changes.

The application discloses a kind of nanowire pH electrode with micro reference and its preparation method and application, belong to test electrode technical field.The electrode structure includes insulating shell, working part and reference part;Working part, reference part is respectively by with liquid metal connection working electrode material, reference electrode material;Working part and reference part are separated by insulating shell assembly, and do not communicate with each other;Working electrode material and reference electrode material are core-shell structure nanowire with silicon carbide as core and surface cladding shell layer, wherein the shell layer of working electrode material is the metal oxide with pH response performance, and the shell layer of reference electrode material is silver-silver chloride compound.The application realizes the real-time dynamics of monitoring intracellular or micro area pH, and realizes reliable detection of intracellular pH, and provides a simple and easy preparation method, breaks the limitation that metal oxide is difficult to be miniaturized and used for intracellular pH detection in electrode preparation, and has wide application prospect.

The invention discloses an Escherichia coli alkali-resistant target spot and application thereof, an inhibition model of pH on Escherichia coli E.coli AG1 is established by setting LB culture media with different pH values, and it is found that the inhibition degree of cell growth is positively correlated with the rise of pH. Under the condition that the pH value is equal to 10, the escherichia coli overexpression library ASKA is subjected to high-throughput screening, and 43 alkali-tolerant elements are obtained after multiple rounds of screening. The obtained gene is subjected to KEGG / GO enrichment analysis through an online gene function annotation database DAVID, an EcoCyc database and related literatures are looked up, an alkali tolerance mechanism is predicted, and it is found that the alkali tolerance mechanism is possibly related to biological membrane formation, intracellular pH steady state maintenance, intracellular ROS level reduction and the like. Through overexpression of the genes, the strain can adapt to an environment under alkali stress, the alkali tolerance of the strain is improved, and the strain has a good application prospect.

Compositions and methods are disclosed herein for inducing (up-regulating) the expression and / or activity of sulfotransferases in the hair follicles, e.g., the scalp. Increasing sulfotransferase is beneficial for metabolizing pro-drugs that require sulfonation to be activated, e.g., minoxidil sulfate is the active metabolite of minoxidil. A method for combining the compositions described herein with topical minoxidil to enhance minoxidil treatment for androgenetic alopecia is described. Additional methods and compositions include the use of retinoid X receptor agonists, retinoic acid receptor agonists, and nuclear receptor agonists in an RXR-NR heterodimer. Additional methods and compositions include the use of a topical solution containing an alkalinizing agent or an alkalinizing agent used with a penetration enhancer for up-regulating the sulfonating capacity of hair bearing skin, hair follicles, and / or keratinocyte cells. In addition, compositions and methods for increasing or decreasing the growth rate of hair follicles is disclosed by altering the intracellular pH. Additionally, methods for preparing a stable liposomal containing solution are described.

The invention discloses a method for relieving tomato pollen high-temperature fertility disorder, and relates to the technical field of biology, and the method specifically comprises the step of overexpressing carbonic anhydrase gene betaCA2.2 in tomatoes or increasing the activity of protein coded by the carbonic anhydrase gene betaCA2.2. Furthermore, carbonic anhydrase gene betaCA2.2 overexpression strains OE: betaCA2.2-1 and OE: betaCA2.2-2 are obtained through screening by using a transgenic technology and a tissue culture technology, compared with wild WT, the overexpression strains have the advantages that the pollen activity at high temperature is remarkably improved, the intracellular pH change is remarkably stable, the tomato pollen fertility disorder caused by high-temperature stress is relieved, and the tomato pollen fertility disorder caused by high-temperature stress is relieved. The tomato fruit setting rate under high-temperature stress is obviously improved, and an important gene resource is provided for cultivating high-temperature-resistant tomato varieties.

Compositions and methods are disclosed herein for inducing (up-regulating) the expression of sulfotransferases in the hair follicles, e.g., the scalp. Increasing sulfotransferase is beneficial for metabolizing pro-drugs that require sulfonation to be activated, e.g., minoxidil sulfate is the active metabolite of minoxidil. A method for combining the compositions described herein with topical minoxidil to enhance minoxidil treatment for androgenetic alopecia is described. Additional methods and compositions include the use of retinoid X receptor agonists, retinoic acid receptor agonists, and nuclear receptor agonists in an RXR-NR heterodimer. Additional methods and compositions include the use of a topical solution containing an alkalinizing agent or an alkalinizing agent used with a penetration enhancer for up-regulating the sulfonating capacity of hair bearing skin, hair follicles, and / or keratinocyte cells. In addition, compositions and methods for increasing or decreasing the growth rate of hair follicles is disclosed by altering the intracellular pH.

The invention belongs to the technical field of single cell detection, and particularly relates to a method for detecting the intracellular pH value in the process of expressing heterologous protein by escherichia coli and application. The detection method comprises the following steps: S1, establishing a pH response curve; s2, co-incubating the fluorescent probe AF-C and the escherichia coli in the process of expressing the heterologous protein to obtain a real-time intracellular cell image of the escherichia coli; s3, processing the real-time cell image in the step S2, and calculating the fluorescence intensity of a Red channel and the fluorescence intensity of an NIR channel corresponding to a single cell; and substituting the ratio of the fluorescence intensity of the Red channel to the fluorescence intensity of the NIR channel into the pH response curve in the step S1 to obtain the intracellular pH value of the escherichia coli. The detection method provided by the invention has good permeability and retention in the process of expressing heterologous protein by escherichia coli, has fast spectral response to intracellular pH change, and is more accurate in detection result.

The application discloses a hydroxyalkyl starch complex and a preparation method and application thereof. The hydroxyalkyl starch complex has a structure as shown in formula I: wherein, HAS' is a molecule of hydroxyalkyl starch without a terminal carbohydrate part; R1', R2' and R3' are independently any one of a group of formula II, a group of formula III, a hydrogen atom or a C1-C8 straight chain / branched chain hydroxyalkyl group, and the proportion of the group of formula II in R1', R2' and R3' is 10%-40%. The complex can exist in the form of nanoparticles, and through modification of a carbonic anhydrase inhibitor, expression of carbonic anhydrase of tumor cells can be inhibited, pH of tumor cells, tumor stem cells and tumor-related fibroblasts can be regulated, intracellular pH can be reduced, catalytic efficiency of chemical kinetics induced by metal ions is favorable, and the killing effect on tumor cells is improved.