25 results about "Intracellular pH" patented technology

The application belongs to the technical field of fluorescent nanomaterials, and provides nitrogen-doped carbon dots, a preparation method thereof and application of the nitrogen-doped carbon dots in colorimetric / fluorescent dual-mode visual detection of pH and histamine, to solve the problem that regular colorimetric signals of current carbon dots are difficult to achieve, which limits the application of the carbon dots in biomedicine and photoelectric devices. The nitrogen-doped fluorescent carbon dots are synthesized by one-step hydrothermal reaction of o-phenylenediamine and 2,6-pyridinedicarboxylic acid, water and hydrochloric acid. The preparation method is simple and fast, the synthesized carbon dots have good water solubility and biocompatibility, stable optical properties, high fluorescence quantum yield, can quickly and visually detect intracellular pH and histamine in food spoilage process, have excellent selectivity and anti-interference ability, fast response speed, high sensitivity, and can realize on-site and instant detection by means of a smart phone and a test strip.

The invention discloses a compound and application thereof in preparation of a living cell nucleus staining reagent. The compound is a compound as shown in a formula (I) or a stereoisomer thereof, and can enter a cell nucleus of a living cell within ten minutes, uniformly dye the cell nucleus and display bright fluorescence, a rinsing step is not needed, and background fluorescence interference outside the cell nucleus does not exist; the method has the advantages of simplicity in operation, low cytotoxicity, good light stability, no influence of intracellular pH value and the like. # imgabs0 #

The application provides a near-infrared fluorescent pH probe and a preparation method and application thereof, and belongs to the technical field of biosensing; the chemical structural formula of the probe is as follows: the novel near-infrared fluorescent pH probe can detect in a more acidic range, for example, when the pH changes from 6.40 to 3.00, the fluorescence intensity at 790 nm is enhanced by nearly 70 times, and the fluorescent effect is better; the fluorescent pH probe has low cytotoxicity, high stability and good biocompatibility; and can effectively perform fluorescence imaging on different intracellular pH.

The invention relates to a new chemogenetic tool manipulating intracellular pH levels and hydrogen sulfide levels in living cells and tissues with high spatio-temporal resolution.

The invention discloses a nano material for targeted regulation of intracellular pH value and a preparation method and application thereof. In order to solve the problem of cell dysfunction caused by pH value imbalance in cells, the nano vesicles based on biomimetic modification of the cell membranes are provided, pH buffer salt is entrapped by the cell membranes of target cells, and the target cells are specifically recognized. The target cells comprise related cells of a motor system, an endocrine system or a digestive system. The pH buffer salt is a phosphate compound, a carbonate compound, a citrate compound or a succinate compound. The preparation method comprises the following steps: extracting a targeted cell membrane through a differential centrifugation method, carrying out ultrasonic fusion on the targeted cell membrane and a buffer salt solution to prepare the nano material, applying the nano material to preparation of drugs for type 2 diabetes, type 2 inflammatory bowel disease or osteoporosis, specifically targeting corresponding target cells, releasing a regulator, and regulating the intracellular pH value of the target cells.

The invention relates to the technical field of gene engineering, and particularly discloses a construction method and application of intracellular stable self-expression pH-sensitive fluorescent protein particles. The recombinant plasmid comprises a coding gene of a pH sensitive fluorescent protein pHlurin and a J23119 promoter; the preparation method comprises the following steps: cloning a pH sensitive fluorescent protein pHlurin gene segment to a pBAD24 plasmid, and replacing an araBAD promoter of the pBAD24 plasmid with J23119; the nucleotide sequence of the pHlurin gene is as shown in SEQ ID No. 1; the nucleotide sequence of the J23119 is as shown in SEQ ID No. 2. The recombinant plasmid constructed by the invention not only realizes absolute quantification of intracellular pH, but also has better detection performance and accuracy; in addition, the application environment of the plasmid is expanded, and continuous, stable and high-expression of the pH-sensitive fluorescent protein can be realized without an inducer.

This invention provides methods to determine the stage in the natural time course of immune response in a subject. It describes method to control immune response by targeting regulatory mechanisms differentially operating in lymphocytes at different stages in the natural time course of immune response. One kind of such regulatory mechanism is the mechanisms of energy metabolism that control intracellular pH (pHi), which in turn control cell death and proliferation. This invention also describes a combinatorial approach to lowering pHi of neoplastic cells to induce their death using compositions of combinations of regulators of energy metabolism. Finally, this invention provides methods to treat skin or mucosa infections or neoplasia by topical application of pH modifier(s) that lower pH.

The invention discloses a method for regulating an environment in a plant cell to be alkaline, and belongs to the technical field of pH regulation in the plant cell. Comprising the following steps: carrying out targeted expression on light-operated hydrogen ion pump protein PPR on cell membranes of plant cells, and then stimulating by green light with the light intensity of 40-500 [mu] W / mm < 2 > and the wavelength of 520-560 nm to adjust the pH in the cells to be alkaline. By selecting the specific photosensitive protein and the cell specific promoter, accurate pH regulation and control of specific cells or organelles can be realized. The PPR gene is fused with a cell membrane targeting sequence, and under the stimulation of specific light, only the pH of cytoplasm is obviously changed, and the pH of other cell regions is not influenced.

The invention discloses a kit for predicting the intracellular pH value of lung adenocarcinoma and the sensitivity of cisplatin treatment as well as a use method and application of the kit. According to the kit disclosed by the invention, the pH value in tumor cells of a patient is judged by detecting the expression quantity of ZNF266 in tumor tissues of the patient with lung adenocarcinoma, and the treatment effect of cis-platinum is predicted; the method is used for predicting the prognosis of a patient before treatment and evaluating the curative effect of a treatment scheme and the prognosis of the patient during and after treatment, so that precise medical treatment is realized. According to the invention, the method for predicting the pH value in the tumor cells of the patient and the cis-platinum treatment sensitivity by detecting the ZNF266 expression is proposed for the first time, the treatment scheme effect can be effectively evaluated, the individualized treatment of the lung adenocarcinoma patient is guided, the clinical benefit is improved, and meanwhile, unnecessary medical resource waste is avoided.

Compositions and methods are disclosed herein for inducing (up-regulating) the expression of sulfotransferases in the hair follicles, e.g., the scalp. Increasing sulfotransferase is beneficial for metabolizing pro-drugs that require sulfonation to be activated, e.g., minoxidil sulfate is the active metabolite of minoxidil. A method for combining the compositions described herein with topical minoxidil to enhance minoxidil treatment for androgenetic alopecia is described. Additional methods and compositions include the use of retinoid X receptor agonists, retinoic acid receptor agonists, and nuclear receptor agonists in an RXR-NR heterodimer. Additional methods and compositions include the use of a topical solution containing an alkalinizing agent or an alkalinizing agent used with a penetration enhancer for up-regulating the sulfonating capacity of hair bearing skin, hair follicles, and / or keratinocyte cells. In addition, compositions and methods for increasing or decreasing the growth rate of hair follicles is disclosed by altering the intracellular pH. Additionally, methods for preparing a stable liposomal containing solution are described.

The invention discloses an embolism emulsion capable of regulating and controlling tumor pH and inducing potent ferroptosis and a preparation method of the embolism emulsion, and belongs to the technical field of biological medicines.The emulsion is prepared by combining surface-modified iron nanoparticles (FeNPs), iodized oil and a sodium-hydrogen exchanger inhibitor (cariposide) through a Pickering emulsion technology; the problems that a traditional iodinated oil emulsion is poor in stability and insufficient in drug slow release are solved. FeNPs is used as a Fenton reaction catalyst, hydroxyl radicals are efficiently generated in a tumor acidic microenvironment, and ferroptosis is induced; the cariposide inhibits a sodium-hydrogen exchange body, reduces the intracellular pH value, relieves extracellular acidification, and synergistically enhances the Fenton reaction activity. The emulsion is administrated through arterial embolism, and can remarkably inhibit tumor growth, activate anti-tumor immunity and reverse an immunosuppression microenvironment. In-vitro and animal experiments show that the compound has excellent stability, slow release characteristic (the drug release speed is reduced by 50%) and treatment efficiency, and is suitable for treating liver cancer through transcatheter arterial chemoembolization (TACE).

The invention relates to a new chemogenetic tool manipulating intracellular pH levels and hydrogen sulfide levels in living cells and tissues with high spatio-temporal resolution.

The invention relates to the technical field of biological detection, in particular to a plant cell adaptation-based pH calibration kit, which comprises a multi-pH buffer solution set, a plant adaptation fluorescent probe, a cell wall enzymolysis reagent, a pH calibration solution and a negative control solution, the multi-pH buffer solution set comprises eight buffer solutions with pH values of 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 6.8 and 7.0, and the plant adaptation fluorescent probe is used for detecting the pH of the plant cell adaptation-based pH calibration kit. A buffer solution in an extreme acid region with the pH value of 4.0-5.5 takes citrate as a main buffer agent, a buffer solution in a weak acid region with the pH value of 5.5-6.5 takes 2-morpholine ethanesulfonic acid as a main buffer agent, and a buffer solution in a neutral region with the pH value of 7.0 takes 4-hydroxyethyl piperazine ethanesulfonic acid as a main buffer agent; through broad-spectrum pH coverage, efficient probe loading and anti-interference design, three core values are provided for plant cell research, multiple technical problems in plant cell pH accurate detection are effectively solved, and a reliable tool is provided for researching intracellular pH dynamics of plants in the processes of photosynthesis regulation, stress response, ion transport and the like.

The application provides an organic room-temperature phosphorescent molecular compound and a preparation method and application thereof. The organic room-temperature phosphorescent molecular compound provided in the application takes a six-substituted arene phosphorescent core as a core molecule, and a group capable of inducing intermolecular hydrogen bonding is modified on the core molecule to form different aggregation states through pi-pi stacking and hydrogen bonding. The preparation method of the compound comprises the following steps: taking a six-substituted benzene as a basis, further synthesizing a six-substituted arene structure as a compound skeleton, and then introducing groups including a carboxyl group, a carboxyl group derivative, a hydroxyl group or an amide on the skeleton. The organic room-temperature phosphorescent molecular compound can be used for cell life imaging and as a pH sensing probe for detecting intracellular pH changes. The compound provided in the application has a life of up to 30 mu s when the water content is 99%, can respond to pH, and provides accurate and reliable life biological imaging in clinical biomedical applications; and when used as a pH sensing probe, the compound can accurately respond to intracellular pH changes.

The application discloses a method for improving the nitrification efficiency under alkaline conditions by regulating extracellular polymeric substances and ion transport. The method induces the secretion of more acidic polysaccharides and acidic organic substances in the extracellular polymeric substances of nitrifying bacteria and regulates the activities of anti-transport proteins and F0-F1 ATPase by gradually increasing the pH value of the system, the operation days and other comprehensive strategies, thereby promoting the intracellular H + input to maintain the intracellular pH stability. At the same time, the biological mineralization effect of carbonic anhydrase is utilized to accelerate the consumption of extracellular OH ‑ , improve the alkaline environmental adaptability and nitrification performance of the nitrification sludge, and significantly enrich Nitrosomonas and Nitrospira and other alkali-tolerant nitrifying bacteria, and the average ammonia nitrogen removal rate of the system under the condition of pH 10.0 can be as high as 96.6%.

The application discloses a kind of nanowire pH electrode with micro reference and its preparation method and application, belong to test electrode technical field.The electrode structure includes insulating shell, working part and reference part;Working part, reference part is respectively by with liquid metal connection working electrode material, reference electrode material;Working part and reference part are separated by insulating shell assembly, and do not communicate with each other;Working electrode material and reference electrode material are core-shell structure nanowire with silicon carbide as core and surface cladding shell layer, wherein the shell layer of working electrode material is the metal oxide with pH response performance, and the shell layer of reference electrode material is silver-silver chloride compound.The application realizes the real-time dynamics of monitoring intracellular or micro area pH, and realizes reliable detection of intracellular pH, and provides a simple and easy preparation method, breaks the limitation that metal oxide is difficult to be miniaturized and used for intracellular pH detection in electrode preparation, and has wide application prospect.

The invention discloses a nano material for inhibiting aseptic inflammation as well as a preparation method and application of the nano material. The nano material is hybrid liposome nano vesicles modified by a platelet membrane, and the mass ratio of protein to lipid is 1: 2. The nano vesicles are loaded with sylvite compounds, can target in-vivo tissue injury parts, release potassium ions under the action of ultrasound, realize intracellular pH regulation and control through hydrogen-potassium exchange, and inhibit aseptic inflammation. The hybrid liposome is formed by mixing phospholipid and cholesterol, and the potassium salt compound is potassium carbonate or potassium bicarbonate. The preparation method comprises the following steps: preparing a hybrid liposome membrane and a platelet membrane, respectively adding a sylvite compound solution to carry out ultrasonic hydration and resuspension washing, preparing hybrid liposome vesicles and platelet membrane vesicles entrapped with sylvite compounds, and carrying out ultrasonic treatment, size stabilization and dialysis to obtain the platelet membrane modified hybrid liposome nano-vesicles. Inflammation can be inhibited in the early stage of injury, and side effects of traditional drugs are avoided.

The invention discloses an Escherichia coli alkali-resistant target spot and application thereof, an inhibition model of pH on Escherichia coli E.coli AG1 is established by setting LB culture media with different pH values, and it is found that the inhibition degree of cell growth is positively correlated with the rise of pH. Under the condition that the pH value is equal to 10, the escherichia coli overexpression library ASKA is subjected to high-throughput screening, and 43 alkali-tolerant elements are obtained after multiple rounds of screening. The obtained gene is subjected to KEGG / GO enrichment analysis through an online gene function annotation database DAVID, an EcoCyc database and related literatures are looked up, an alkali tolerance mechanism is predicted, and it is found that the alkali tolerance mechanism is possibly related to biological membrane formation, intracellular pH steady state maintenance, intracellular ROS level reduction and the like. Through overexpression of the genes, the strain can adapt to an environment under alkali stress, the alkali tolerance of the strain is improved, and the strain has a good application prospect.

The invention discloses an acidification hydrolysis method for improving the yield of hermetia illucens small molecule peptides and application. The acidification hydrolysis method comprises the following steps: firstly, carrying out acidification pretreatment on hermetia illucens bodies, and adding protease for hydrolysis treatment under an acidic condition; by means of acidic hydrolysis, on one hand, bacterial cell membrane proton gradient imbalance can be achieved, membrane protein of bacterial cell membranes is inactivated, organic acid can penetrate through bacterial cell membranes, intracellular dissociation is achieved, intracellular pH is reduced, ATP synthesis is inhibited, then microbial activity is inhibited, and sterilization of bacteria in hermetia illucens is achieved; on the other hand, hydrogen bonds and salt bridges in the hermetia illucens protein can be destroyed by the acid environment, so that a compact spherical structure of the protein is expanded, more enzyme cutting sites (especially hydrophobic residues) are exposed, and more small molecule peptide fragments are obtained. Through tests, small peptides with the molecular weight of 729 Da to 246 Da in the hydrolyzed product account for 73.03% of the total content.

Compositions and methods are disclosed herein for inducing (up-regulating) the expression and / or activity of sulfotransferases in the hair follicles, e.g., the scalp. Increasing sulfotransferase is beneficial for metabolizing pro-drugs that require sulfonation to be activated, e.g., minoxidil sulfate is the active metabolite of minoxidil. A method for combining the compositions described herein with topical minoxidil to enhance minoxidil treatment for androgenetic alopecia is described. Additional methods and compositions include the use of retinoid X receptor agonists, retinoic acid receptor agonists, and nuclear receptor agonists in an RXR-NR heterodimer. Additional methods and compositions include the use of a topical solution containing an alkalinizing agent or an alkalinizing agent used with a penetration enhancer for up-regulating the sulfonating capacity of hair bearing skin, hair follicles, and / or keratinocyte cells. In addition, compositions and methods for increasing or decreasing the growth rate of hair follicles is disclosed by altering the intracellular pH. Additionally, methods for preparing a stable liposomal containing solution are described.

The present invention discloses a pH ratio change fluorescent probe based on the FRET mechanism. The probe is composed of three parts: an energy donor coumarin derivative fluorophore, a linker piperazine, and an energy acceptor (E)-3-(2-hydroxyethyl)-1,1-dimethyl-2-(4-methylphenyl)-1H-benz[e]indole-3-ium bromide. The probe is a spiropyran compound, and its chemical structure is shown in Formula I. The fluorescent probe prepared by the present invention is based on the FRET mechanism. As the pH value increases, the fluorescence intensity at 608nm gradually decreases, and the fluorescence intensity at 485nm gradually increases. Fluorescence intensity ratio I 485 / I 608 It shows a good linear relationship within the pH range of 7.2 to 8.0, and is expected to play a role in detecting changes in solution systems and intracellular pH values, and has broad application prospects.

The invention discloses a method for relieving tomato pollen high-temperature fertility disorder, and relates to the technical field of biology, and the method specifically comprises the step of overexpressing carbonic anhydrase gene betaCA2.2 in tomatoes or increasing the activity of protein coded by the carbonic anhydrase gene betaCA2.2. Furthermore, carbonic anhydrase gene betaCA2.2 overexpression strains OE: betaCA2.2-1 and OE: betaCA2.2-2 are obtained through screening by using a transgenic technology and a tissue culture technology, compared with wild WT, the overexpression strains have the advantages that the pollen activity at high temperature is remarkably improved, the intracellular pH change is remarkably stable, the tomato pollen fertility disorder caused by high-temperature stress is relieved, and the tomato pollen fertility disorder caused by high-temperature stress is relieved. The tomato fruit setting rate under high-temperature stress is obviously improved, and an important gene resource is provided for cultivating high-temperature-resistant tomato varieties.

Compositions and methods are disclosed herein for inducing (up-regulating) the expression of sulfotransferases in the hair follicles, e.g., the scalp. Increasing sulfotransferase is beneficial for metabolizing pro-drugs that require sulfonation to be activated, e.g., minoxidil sulfate is the active metabolite of minoxidil. A method for combining the compositions described herein with topical minoxidil to enhance minoxidil treatment for androgenetic alopecia is described. Additional methods and compositions include the use of retinoid X receptor agonists, retinoic acid receptor agonists, and nuclear receptor agonists in an RXR-NR heterodimer. Additional methods and compositions include the use of a topical solution containing an alkalinizing agent or an alkalinizing agent used with a penetration enhancer for up-regulating the sulfonating capacity of hair bearing skin, hair follicles, and / or keratinocyte cells. In addition, compositions and methods for increasing or decreasing the growth rate of hair follicles is disclosed by altering the intracellular pH.

The invention belongs to the technical field of single cell detection, and particularly relates to a method for detecting the intracellular pH value in the process of expressing heterologous protein by escherichia coli and application. The detection method comprises the following steps: S1, establishing a pH response curve; s2, co-incubating the fluorescent probe AF-C and the escherichia coli in the process of expressing the heterologous protein to obtain a real-time intracellular cell image of the escherichia coli; s3, processing the real-time cell image in the step S2, and calculating the fluorescence intensity of a Red channel and the fluorescence intensity of an NIR channel corresponding to a single cell; and substituting the ratio of the fluorescence intensity of the Red channel to the fluorescence intensity of the NIR channel into the pH response curve in the step S1 to obtain the intracellular pH value of the escherichia coli. The detection method provided by the invention has good permeability and retention in the process of expressing heterologous protein by escherichia coli, has fast spectral response to intracellular pH change, and is more accurate in detection result.

The application discloses a hydroxyalkyl starch complex and a preparation method and application thereof. The hydroxyalkyl starch complex has a structure as shown in formula I: wherein, HAS' is a molecule of hydroxyalkyl starch without a terminal carbohydrate part; R1', R2' and R3' are independently any one of a group of formula II, a group of formula III, a hydrogen atom or a C1-C8 straight chain / branched chain hydroxyalkyl group, and the proportion of the group of formula II in R1', R2' and R3' is 10%-40%. The complex can exist in the form of nanoparticles, and through modification of a carbonic anhydrase inhibitor, expression of carbonic anhydrase of tumor cells can be inhibited, pH of tumor cells, tumor stem cells and tumor-related fibroblasts can be regulated, intracellular pH can be reduced, catalytic efficiency of chemical kinetics induced by metal ions is favorable, and the killing effect on tumor cells is improved.