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The present application relates to the field of plant tissue culture, and discloses a high-efficiency accumulation of paeoniflorin in vitro culture method of Paeonia plant. Through in-vitro domestication, bud induction, absorption surface construction, adventitious bud induction, content and biomass double-increase culture, a high-efficiency in-vitro regeneration system of Paeonia plant is established, and on this basis, high-efficiency accumulation of paeoniflorin is realized, so that the yield of paeoniflorin is significantly improved, a new technical approach for large-scale and standardized production of paeoniflorin is provided, and the present application has important application value and industrialization prospect.

The application discloses a kind of based on two-dimensional weighted back projection and linear base function interpolation trunk three-dimensional imaging method, comprising: selecting several different measurement heights of to-be-imaged trees, using stress wave measuring equipment respectively at different heights to carry out stress wave data acquisition;Using two-dimensional weighted back projection algorithm reconstructs the stress wave velocity field of each layer, obtains the control point set needed for linear base function interpolation;All known control point set is mapped to three-dimensional coordinate system, and with the wave speed v corresponding to each point constitutes three-dimensional feature point cloud;Linear base function interpolation of using linear kernel is used to carry out interpolation reconstruction, and function F ( x , y , z ) are fitted;According to fitting function F ( x , y , z ) all point sets located in three-dimensional fault imaging region are calculated;Using the display method of point cloud, the points inside imaging region are visualized and displayed.The application significantly improves three-dimensional imaging speed and defect recognition accuracy in actual scene, and is suitable for nondestructive testing and evaluation of three-dimensional defects inside trunk.

This invention discloses an amidase gene for degrading amide alcohol antibiotics and amide herbicides. right here The amidase gene, along with its encoded protein and applications, has the nucleotide sequence SEQ ID NO.1, a full length of 912 bp, encoding 303 amino acids, and its amino acid sequence is SEQ ID NO.2. This invention relates to the gene... right here This is the first publicly disclosed amidase gene for amide alcohol antibiotics cloned from the genome of a single isolated strain. The amidase encoded by this gene can not only hydrolyze the amide bonds of amide alcohol antibiotics, but also degrade various amide chemical herbicides such as propargite, bromoxynil, chlorpyrifos, and chlorobromopropylate. The amidase encoded by this gene, as well as genetically engineered bacteria containing the amidase gene, have very important theoretical and applied value in the degradation of amide alcohol antibiotic pollutants in aquatic environments such as sewage treatment plants and livestock and poultry breeding wastewater, and in the remediation of herbicide-contaminated soil.

A polypeptide targeting integrin α6 and the use thereof. The polypeptide has a sequence motif of RWYD, and is stronger in affinity, wherein the equilibrium dissociation constant KD reaches the level of nM, and the affinity is greatly improved. The polypeptide has a strong affinity to integrin α6, a strong specificity and no biological toxicity, is relatively stable in vivo and reasonable in distribution, and can be used as a targeting carrier. In addition, the polypeptide targeting integrin α6 can be used as a molecular probe in the molecular imaging of various tumors with highly-expressed integrin α6. Further provided is a dimer of the polypeptide, which has a more significant targeting effect and no biological toxicity. The polypeptide targeting integrin α6 can be used in the molecular imaging of various tumors with highly-expressed integrin α6, and has an important application value in the molecular imaging of tumors.