21 results about "Ph sensing" patented technology

The present invention relates generally to systems for measuring pH. In particular, the present invention relates to a continuous flow system having one or more solid state pH sensors positioned within a fluid pathway of the system to provide continuous pH measurement.

This invention discloses a target-gated ratiometric pH sensor based on a dual CRISPR system of tetrahedral framework nucleic acids, belonging to the fields of biomedicine and nucleic acid nanotechnology. The sensor uses tetrahedral framework nucleic acids as a rigid scaffold, precisely assembling Cas12a, Cas13a, and their corresponding fluorescent reporter molecules in a 1:1 stoichiometric ratio. Their orthogonal pH responses form the basis of the ratiometric sensing. Target recognition-pH sensing logic gating is achieved through target-specific crRNA design. Cholesterol modification allows for stable anchoring on the lipid membrane surface. This invention also discloses the sensor's fabrication, membrane anchoring, pH monitoring, and target-gated function implementation methods. This sensor possesses advantages such as high-precision ratio measurement, accurate spatial arrangement, high specificity, stable membrane anchoring, and a wide pH response range, and can be used for precise monitoring of pH dynamics at biomembrane interfaces and during extracellular vesicle fusion.

The application discloses a kind of pH-sensitive spectrum response film online monitoring methods based on chemical crosslinking.It is technical solution: first polyvinyl alcohol is dissolved in water, then with cellulose solution, pH indicator is mixed according to certain proportion, prepare pH-sensitive original solution, then it is drop-coated to amino glass substrate and incubated, prepare pH-sensitive substrate.Using etching or molding method to make cover piece with microchannel, it is bonded after being treated with oxygen plasma with pH-sensitive substrate, obtain pH microfluidic chip.And it is used in combination with self-built integrated pH sensing system.Self-built integrated pH sensing system includes light source (1), pH sensing chip (2), spectrometer or colorimeter (3) and host computer (4).This pH microfluidic chip online monitoring system based on chemical crosslinking has long service life, wide pH response range, strong anti-interference ability, good stability and other advantages, and can realize online, real-time monitoring of pH.

The invention belongs to the technical field of molecular photoswitch materials, and provides a pyrazine coordination ruthenium complex, a preparation method thereof and application of the pyrazine coordination ruthenium complex as a ratio type molecular photoswitch, the pyrazine coordination ruthenium complex is composed of positive ions and negative ions, the structural formula of the positive ions is shown in the formula (I), and the negative ions are shown in the specification. The preparation method comprises the following steps: firstly, reacting 2-acetylpyrazine with benzimidazole-2-formaldehyde to prepare a ligand L; and filtering after the reaction, adding a ligand L into the filtrate for heating reflux reaction, and purifying the product to obtain the pyrazine coordination ruthenium complex. The complex provided by the invention has unique dual emission and ratio type response, can realize high-precision and anti-interference ratio type pH sensing, and eliminates the influence of factors such as probe concentration, instrument efficiency and the like.

The present invention relates generally to systems for measuring pH. In particular, the present invention relates to a continuous flow system having one or more solid state pH sensors positioned within a fluid pathway of the system to provide continuous pH measurement.

The invention discloses a targeted gating ratio type pH sensor of a double-CRISPR system based on tetrahedral framework nucleic acid, and belongs to the technical field of biomedicine and nucleic acid nanotechnology. According to the sensor, tetrahedral framework nucleic acid is used as a rigid support, Cas12a, Cas13a and corresponding fluorescent reporter molecules are accurately assembled according to the stoichiometric ratio of 1: 1, and orthogonal pH responses of the Cas12a and the Cas13a form a ratio type sensing basis; target recognition-pH sensing logic gating is realized through target specific crRNA (Complementary Ribonucleic Acid) design; after being modified by cholesterol, the compound can be stably anchored on the surface of a lipid membrane. The invention also discloses a preparation method of the sensor, a membrane anchoring method, a pH monitoring method and a target gating function realization method. The sensor has the advantages of high-precision ratio measurement, accurate spatial arrangement, high specificity, stable membrane anchoring, wide pH response range and the like, and can be used for accurately monitoring pH dynamics in a biological membrane interface microenvironment and extracellular vesicle fusion process.

This invention discloses a method for preparing Au@1,4-BDT@Au@4-MBA core-shell structured nanoparticles with sub-nanometer gaps. The method primarily uses a thiol molecule with a benzene ring as an internal standard, specifically 1,4-benzenedithiophenol (1,4-BDT), precisely encapsulated within the gap between a gold core and a gold shell. The gold core has a diameter of 35–40 nm, and the gold shell has a diameter of 45–60 nm, resulting in an overall particle size of 80–100 nm. The internal standard molecule located in the sub-nanometer gap layer generates a very strong electromagnetic enhancement signal, and because it is physically isolated by the outer gold shell layer, its signal is unaffected by external pH environments or solution matrices, providing an excellent reference. An external standard molecule (4-MBA) is then used for the outermost coating, serving as an acid-base sensing unit. A ratiometric calibration model is established based on the ratio of its characteristic peak to the internal standard peak, effectively reducing errors caused by uneven hotspots and matrix interference. This material can be applied to pH detection of human sweat based on SERS technology.

The application provides an organic room-temperature phosphorescent molecular compound and a preparation method and application thereof. The organic room-temperature phosphorescent molecular compound provided in the application takes a six-substituted arene phosphorescent core as a core molecule, and a group capable of inducing intermolecular hydrogen bonding is modified on the core molecule to form different aggregation states through pi-pi stacking and hydrogen bonding. The preparation method of the compound comprises the following steps: taking a six-substituted benzene as a basis, further synthesizing a six-substituted arene structure as a compound skeleton, and then introducing groups including a carboxyl group, a carboxyl group derivative, a hydroxyl group or an amide on the skeleton. The organic room-temperature phosphorescent molecular compound can be used for cell life imaging and as a pH sensing probe for detecting intracellular pH changes. The compound provided in the application has a life of up to 30 mu s when the water content is 99%, can respond to pH, and provides accurate and reliable life biological imaging in clinical biomedical applications; and when used as a pH sensing probe, the compound can accurately respond to intracellular pH changes.

Described is a versatile surface modification approach to, for example, modularly and orthogonally functionalize nanoparticles (NPs) such as, for example, PEGylated nanoparticles, ith various types of different functional ligands (functional groups) on the NP surface. It enables the synthesis of, for example, penta-functional PEGylated nanoparticles integrating a variety of properties into a single NP, e.g., fluorescence detection, specific cell targeting, radioisotope chelating / labeling, ratiometric pH sensing, and drug delivery, while the overall NP size remains, for example, below 10 nm.

The invention discloses a silver nano-cluster and a preparation method and application thereof, belongs to the technical field of metal cluster materials, and aims to synthesize Ag NCs by taking PEI as a template and ascorbic acid as a reducing agent. The invention also discloses an application of the silver nanocluster in pH sensing. As the pH sensor, the Ag NCs provided by the invention can show obvious fluorescence intensity and absorbance changes along with the change of acidity, so that the Ag NCs can combine the advantages of high sensitivity of fluorescence detection and simplicity, convenience, rapidness and the like of a colorimetric method, and has more obvious advantages compared with other nanoscale pH sensors.

The invention relates to the technical field of animal breeding and veterinary diagnosis, and provides pH-viscosity bimodal rapid diagnosis test paper and application thereof in sow oestrus identification. The pH-viscosity bimodal rapid diagnosis test paper provided by the invention comprises a base material layer as well as a pH sensing layer and a viscosity sensing layer which are respectively arranged on the surface of the base material layer, the pH sensing layer comprises an acid-base indicator and a film forming material; and the viscosity sensing layer is made of alginate gel. The pH sensing module and the viscosity sensing module are integrated on the same test strip, so that one-time sampling and double-index synchronous detection are realized. The test paper provided by the invention integrates the advantages of rapidness, double indexes and low cost, through physiological index complementary verification, the accuracy, reliability and field applicability of sow oestrus identification are remarkably improved, and the test paper is suitable for sow oestrus field rapid identification.

The invention relates to the technical field of medical detection, in particular to a colorimetric sensing chip for detecting lung cancer markers as well as a preparation method and application of the colorimetric sensing chip. Comprising a filter paper substrate, and an aromatic aldehyde gas sensing identification assembly, an alcohol ester gas sensing identification assembly, an aldehyde ketone gas sensing identification assembly, a first pH sensing assembly and a second pH sensing assembly which are integrated on the filter paper substrate, the aromatic aldehyde gas sensing recognition assembly is modified by gold superparticles GSPs; the alcohol ester gas sensing recognition assembly, the aldehyde ketone gas sensing recognition assembly, the first pH sensing assembly and the second pH sensing assembly are all modified by porous ZIF-8 nanoparticles; the colorimetric sensing chip disclosed by the invention is combined with various gas sensing components, is used for quantitatively detecting various lung cancer markers in exhaled gas by observing color change, can be realized without fluorescent labeling and expensive instruments, can finish rapid detection within 10 minutes through portable equipment such as a mobile phone and the like, has the sensitivity of 50ppb and improves the detection accuracy.

The application discloses a flexible wearable long-term stable ruthenium oxide pH sensing electrode, and belongs to the technical field of sensing and detection. The ruthenium oxide pH sensing electrode comprises, from bottom to top, a flexible substrate layer, an electrode, a pH sensitive layer and a polymer layer. The pH sensitive layer is composed of ruthenium oxide, conductive fillers and a polymer. The polymer layer is combined to enhance the electrode performance. The structures of the electrode can be processed by a drop coating method or a printing method, avoiding complex and harsh processing procedures. The electrode is suitable for electrode processing on common flexible materials, has good processing consistency, and is expected to realize batch production. In the use process, the electrode is combined with simple and convenient electrode electroactivation treatment, and the electrode shows excellent sensitivity in the detection of the pH in the physiological range, shows good storage and use stability, can meet the application requirement of long-time continuous monitoring of body fluid by a flexible wearable sensor, and has wide practical application potential.

A nasogastric tube contains: a body, a plug, and a pH (potential of hydrogen) sensor. The body includes a feeding portion, a discharging portion, and a conduit. The feeding portion has a cap, and the discharging portion has multiple through orifices. The plug includes a connecting portion and a protruded portion. The protruded portion has a smooth surface. The pH sensor is injection molded to integrate into the plug, and the pH sensor includes a pH sensing element and two antennas. The pH sensing element is made of conducting material which is configured to produce different voltage response with a pH ion concentration in gastric juice. The pH value of the gastric juice measured by the pH sensing element is wirelessly send by the two antennas to a reader, and the pH value of the gastric juice measured is displayed on the reader.

The invention provides a medical wound seepage patch and a preparation method thereof, and relates to the technical field of medical supplies. Comprising a one-way moisture absorption layer, a multi-layer water absorption structure layer and a backing layer, a metering color developing strip is arranged in the multi-layer water absorption structure layer, and a pH response color developing assembly is arranged on the side, away from the one-way moisture absorption layer, of the multi-layer water absorption structure layer; the multi-layer water absorption structure layer comprises a primary water absorption layer, a middle water absorption layer and a high water absorption layer which are stacked in sequence, and the primary water absorption layer is provided with flow guide column cotton which vertically penetrates through the middle water absorption layer; the pH response developing assembly comprises a pH sensing film and a transparent observation window, and the pH sensing film is loaded with a pH indicator; the one-way moisture absorption layer is formed by compounding a spunbond non-woven fabric surface layer, a fluffy cotton middle layer and a PE leakage-proof film bottom layer, and the spunbond non-woven fabric surface layer is provided with conical liquid guide holes. According to the seepage patch, one-way moisture absorption of the wound dressing can be achieved, the water absorption amount is large, the seepage absorption amount can be estimated, seepage properties can be judged, the replacement frequency of the wound dressing is reduced, resources are saved, and medical staff are assisted in evaluating the illness state.

The utility model discloses a multi-parameter sensing simple device for monitoring underground water in a karst area, which comprises a sensing box, a water outlet and a water inlet are respectively arranged at the upper ends of bottom plates on the left side and the right side of the sensing box, and a water level sensing line, a water temperature sensing line, a Ph sensing line, a TDS sensing line and a Do sensing line are sequentially arranged in the sensing box. The water level sensing line, the water temperature sensing line, the Ph sensing line, the TDS sensing line and the Do sensing line are connected with a line integration end in the sensing box, inductive probes are arranged at the bottoms of the water level sensing line, the water temperature sensing line, the Ph sensing line, the TDS sensing line and the Do sensing line, and the other end of the line integration end is connected with a display panel. According to the utility model, key parameters such as water level, water temperature, acidity and alkalinity, mineralization degree and the like of underground water are synchronously acquired, and multi-dimensional monitoring data support is provided.

The utility model provides a grease wastewater acid value self-regulation system, which is characterized in that a first control pipeline and a second control pipeline are arranged in parallel between a sulfuric acid source and an oil removal tank, a PH sensing probe is arranged in the oil removal tank to detect the acidity in the oil removal tank in real time, and the control system compares the pH value fed back by the PH sensing probe with a preset pH value to regulate the acid value of the grease wastewater. Opening and closing of the first control pipeline and the second control pipeline are controlled. The first control pipeline is a main supplement flow path of sulfuric acid and is provided with a first electric stop valve and a needle valve, when a small amount of sulfuric acid needs to be added into the oil removal tank, the first control pipeline is independently started, and the needle valve can accurately control the amount of the added sulfuric acid; the first control pipeline is a secondary supplement flow path of sulfuric acid, when a large amount of sulfuric acid needs to be added into the oil removal pool, the first control pipeline and the second control pipeline are started at the same time, the needed sulfuric acid is rapidly supplemented on the premise that the working capacity of the needle valve is not affected, and then the purpose of finely regulating and controlling the amount of the sulfuric acid is achieved.

Systems and methods for measuring pH of a drilling fluid in a downhole drilling environment are disclosed. The system includes wireless pH sensing devices dispersed in the drilling fluid and circulated through a borehole. The wireless pH sensing devices include a capsule shell for protecting internal electronics from harsh downhole conditions. The electronics include an electrolyte insulator semiconductor field-effect (EIS) pH sensor structure, a controller, RF communications unit and power supply. The pH sensing devices periodically measure the pH of the fluid while circulating through the borehole. Upon return of the sensors above ground, the pH measurements are wirelessly provided to a monitoring computer, prior to recirculating the sensors through the borehole.

The invention discloses a flexible wound surface detection dressing integrated with a multi-mode sensor. The flexible wound surface detection dressing can be used for monitoring a wound surface microenvironment. The wound sensor module is printed on the nanofiber membrane module and is attached to the surface of a wound, and the wound sensor module is finally connected with the power supply module through the signal conversion module, the signal transmission module and the signal storage module in sequence. In the wound sensor module, a humidity sensing module, a pH sensing module and an H2O2 sensing module are all printed on the front surface of a nanofiber membrane substrate; the humidity sensing module is an interdigital electrode plated with silver ammonia ink, the pH sensing module comprises a reference electrode and a working electrode plated with a polyaniline film, and the H2O2 sensing module comprises a counter electrode and a working electrode plated with a Prussian blue film. According to the sensing device, the detection of the humidity, the pH and the H2O2 of the wound is jointly realized through the resistance change of the silver-ammonia ink, the potential difference change of the polyaniline electrode and the current generation of the Prussian blue electrode.

A pH sensor comprising pH sensing instrumentation, reference sensing instrumentation, an electrochemical junction, and a pH determination unit. The pH sensing instrumentation comprises a pH fluid, a pH fluid chamber, a pH sensing chamber, and a measuring electrode extending through the pH fluid chamber and comprising a tip that resides in the pH sensing chamber. The reference sensing instrumentation comprises a reference fluid, a reference chamber filled with the reference fluid, a reference electrode immersed in the reference fluid, and monitoring instrumentation. The monitoring instrumentation comprises a monitoring fluid, a monitoring fluid chamber, a monitoring sensing chamber filled with the monitoring fluid, and a monitoring electrode extending through the monitoring fluid chamber and comprising a tip that resides in the monitoring sensing chamber. The monitoring fluid chamber and the monitoring sensing chamber are immersed in the reference fluid.

A smart water bottle includes a liquid level sensor operable to generate sensor data regarding the volume of water in the bottle and transmit the data to at least one external server or device. The liquid level sensor is integrated into the cap and is a time of flight sensor operable to determine the distance of the surface of the water from the cap in order to determine volume. The smart water bottle further includes one or more pH sensors and / or total dissolved solids (TDS) sensors integrated into the cap. Advantageously, the cap is able to be detached from the cradle and nested within a cradle to begin inductive charging in order to increase longevity of the device.