Biological reaction kettle and method for promoting proliferation and reddening of haematococcus pluvialis

A biological reactor and the technology of the reactor are applied in the field of promoting Haematococcus pluvialis proliferation and promoting redness, and can solve the problems of unstable temperature, low output per unit area, low population density, etc., and achieve stable growth. poor performance, increased astaxanthin content, and shortened growth cycle

Active Publication Date: 2017-08-29
ANHUI DEBAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main factors hindering the large-scale production of Haematococcus pluvialis are the biological pollution and the low population density in the cultivation process of Haematococcus pluvialis, which leads to low yield per unit area and high cost.
The defects of Haematococcus pluvialis cultivated in pipeline-type photobioreactors or bioreactors composed of small-scale systems are that the walls of the pipeline are easily adhered by algae cells, resulting in decreased light transmittance, unstable temperature and difficult cleaning during the cultivation period. Therefore, it is only suitable for small-scale production in the laboratory

Method used

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  • Biological reaction kettle and method for promoting proliferation and reddening of haematococcus pluvialis
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  • Biological reaction kettle and method for promoting proliferation and reddening of haematococcus pluvialis

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Experimental program
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Effect test

Embodiment 1

[0031] The method for promoting the proliferation of Haematococcus pluvialis and promoting redness by using the above-mentioned bioreactor, its main steps are as follows:

[0032] 1) Establish an adjustable fully enclosed LED vertical light energy bioreactor, using opaque materials and built-in, diving, adjustable spectrum and light energy LED light energy facilities on the top, bottom, surroundings and cover of the main reactor; The diameter of the main reactor 1 is set to 16cm, the height is set to 160cm, the solution volume is 22 liters, the vertical cylinder body adopts transparent acrylic tube, the bottom of the tube and the upper cover are respectively sealed with plastic plugs, and a sampling port is opened on the side of the cylinder body And the probe port, the bottom of the cylinder has a drain port, and the top of the cylinder has a water inlet and a line inlet;

[0033] 2) A 60W LED lamp tube 2 with adjustable wavelengths and photon flux density is installed in the...

Embodiment 2

[0037] The method for promoting the proliferation of Haematococcus pluvialis and promoting redness by using the above-mentioned bioreactor, its main steps are as follows:

[0038] 1) Establish an adjustable fully enclosed LED vertical light energy bioreactor, using opaque materials and built-in, diving, adjustable spectrum and light energy LED light energy facilities on the top, bottom, surroundings and cover of the main reactor; Among them, the diameter of the main reactor is set to 16cm, the height is set to 160cm, and the solution volume is 22 liters. The vertical cylinder body adopts transparent acrylic tubes, and the bottom and upper cover of the tubes are respectively sealed with plastic plugs. There are sampling ports and The probe port and the bottom of the cylinder are provided with a drain port, and the top of the cylinder is provided with a water inlet and a wire inlet;

[0039] 2) Install 100W LED tubes with adjustable wavelengths and photon flux density in the mai...

Embodiment 3

[0043] The method for promoting the proliferation of Haematococcus pluvialis and promoting redness by using the above-mentioned bioreactor, its main steps are as follows:

[0044] 1) Establish an adjustable fully enclosed LED vertical light energy bioreactor, using opaque materials and built-in, diving, adjustable spectrum and light energy LED light energy facilities on the top, bottom, surroundings and cover of the main reactor; Among them, the diameter of the main reactor is set to 35cm, the height is set to 160cm, and the solution volume is 100 liters. The vertical cylinder body adopts transparent acrylic tubes, and the bottom and upper cover of the tubes are respectively sealed with plastic plugs. There are sampling ports and The probe port and the bottom of the cylinder are provided with a drain port, and the top of the cylinder is provided with a water inlet and a wire inlet;

[0045] 2) Install 500W LED tubes with adjustable wavelengths and photon flux densities in the ...

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Abstract

The invention discloses a biological reaction kettle and a method for promoting proliferation and reddening of haematococcus pluvialis. The biological reaction kettle comprises a main body reaction kettle, wherein the main body reaction kettle is provided with a pH (potential of hydrogen) sensing head interface; the pH sensing head interface is connected with a pH controller; one end of the pH controller is connected with an industrial carbon dioxide bottle, and the other end of the pH controller is connected with the main body reaction kettle through an air intake adjusting valve; a filter is arranged at an outlet of the industrial carbon dioxide bottle; an LED (light emitting diode) lamp tube is arranged in the main body reaction kettle, and is connected with an LED driving controller; gas explosion stone is arranged at the bottom wall of the inner side of the main body reaction kettle, and is connected with a T-level filter through the air intake adjusting valve; the T-level filter is connected with an industrial air compressor; the LED lamp tube is connected with one end of a water cooling machine through the air intake adjusting valve, and the other end of the water cooling machine is connected with the LED lamp tube. The biological reaction kettle has the advantages that the problem of adverse influence by weather, light ray, pollution, environment temperature and the like in the existing traditional microalgae culture type is solved, and the growth speed of microalgae is greatly improved.

Description

technical field [0001] The invention relates to the technical field of coccus growth and cultivation, in particular to a bioreactor and a method for promoting the proliferation and redness of Haematococcus pluvialis. Background technique [0002] Haematococcus pluvialis is a single-celled green alga with oval or ovoid cells ranging in size from 5 microns to around 40-50 microns, or sometimes even larger; two flagella are used to propel the cells to swim freely There is a gap between the cell wall and the protoplast, which is filled with gelatinous substances, and there is a cytoplasm connection between the cell wall and the protoplast. Vegetative reproduction is cell division, forming 2, 4, 8, 16, 32 or even more daughter cells. Asexual reproduction is affected by the environment. Gel colonies or chlamydospores are formed under stress. Haematococcus pluvialis cells in the growth and proliferation stage are green cells; when environmental conditions change unfavorable to its...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/34C12M1/04C12M1/02C12M1/00C12N1/12C12P23/00C12R1/89
CPCC12M21/02C12M29/04C12M29/08C12M29/26C12M31/10C12M41/06C12M41/24C12M41/26C12M41/34C12M41/48C12N1/12C12P23/00
Inventor 蔡明翰约翰雷曼
Owner ANHUI DEBAO BIOTECH CO LTD
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