11 results about "Glucose binding" patented technology

Provided are methods for treating a disorder characterized by elevated blood glucose levels in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a hyperimmunized egg product obtained from an egg-producing animal, wherein the hyperimmunized egg product comprises a therapeutically effective amount of one or more IgY antibodies to an extracellular glucose binding domain of a sodium-glucose co-transporter SGLT. Also provided are hyperimmunized eggs and egg products produced by an animal that has been hyperimmunized with an antigen that contains an extracellular glucose binding domain of a sodium-glucose co-transporter SGLT or fraction thereof, and use of the hyperimmunized eggs or hyperimmunized egg products to reduce blood glucose levels in a subject, such as to treat type 2 diabetes. Methods or preparing the hyperimmunized eggs and hyperimmunized egg products are also provided.

A molecular conjugate comprising a glucose binding macrocycle as represented below that is covalently bonded directly or via linkers to a polypeptide such as insulin to form a glucose sensing insulin (GSI) are described herein. The disclosure includes pharmaceutical compositions, use and method of treatments with such GSIs. Substituents and the various moieties of the structures below are further specified herein. (II) or (III)

The present application discloses a polymer, which comprises a biphenyl boronic acid structure unit capable of binding with glucose and a hydrophilic polymerizable structure unit, wherein the biphenyl boronic acid structure unit can improve the binding capacity and selectivity to glucose. The present application also discloses an insulin sustained-release preparation comprising the above polymer, which has different insulin release rates under different glucose concentrations, can accelerate the release of insulin to reduce blood glucose in a hyperglycemic environment, and can reduce the incorrect release of insulin caused by fructose and other diol substances. The present application further discloses a preparation method of the above insulin sustained-release preparation, which self-assembles the negatively charged insulin and the positively charged biphenyl boronic acid modified polymer into the insulin sustained-release preparation through electrostatic interaction, and has the characteristics of high loading rate. The prepared insulin sustained-release preparation can release insulin, and the released insulin can bind with the insulin receptor in the body to reduce the blood glucose level.

The present application discloses a phenyl boronic acid compound, which contains a phenyl boronic acid group and a carbon-carbon double bond group, and the phenyl boronic acid group can be combined with glucose. The present application also discloses a polymer, which is obtained by free radical copolymerization of the above-mentioned phenyl boronic acid compound, a monomer containing A and an initiator in a solvent. The present application further discloses an insulin sustained-release preparation containing the above-mentioned polymer, which has different insulin release rates under different glucose concentrations, and can accelerate the release of insulin under a hyperglycemic environment to reduce blood glucose. The present application further discloses a preparation method of the above-mentioned insulin sustained-release preparation, which self-assembles the negatively charged insulin and the positively charged double-phenyl boronic acid modified polymer into the insulin sustained-release preparation through electrostatic interaction, and has the characteristics of high loading rate. The prepared insulin sustained-release preparation can release insulin, and the released insulin can bind to insulin receptors in the body to reduce blood glucose level.

The invention discloses a glucose sensor based on a U-shaped superfine optical fiber and a preparation method of the glucose sensor, and relates to the technical field of sensors. The glucose sensor comprises a U-shaped superfine optical fiber, the surface of a sensing area of the U-shaped superfine optical fiber is modified with an amino-containing silane coupling agent, and the silane coupling agent is grafted with glucose oxidase; when light passes through the bent section of the U-shaped superfine optical fiber, due to the fact that the total reflection condition is damaged, part of light energy leaks into the external environment in an evanescent wave mode, when glucose oxidase modified on the surface of the optical fiber is combined with glucose, local refractive index changes are caused, and the phase of the evanescent wave changes. And the concentration of glucose can be calculated by detecting the offset of the interference spectrum. According to the glucose sensor based on the U-shaped superfine optical fiber, the sensitivity of glucose detection is remarkably improved, and the glucose sensor is small in size, convenient to carry or detect and suitable for human body blood glucose detection.

The subject matter of this invention is directed towards insulin analogs that are stable and glucose-responsive. An insulin analog can be a single chain insulin (SCI). The binding affinity of insulin to the insulin receptor can be controlled by the glucose-bound conformation of insulin. The invention further discloses methods for the recombinant expression, purification, and refolding of an insulin analog.

To provide a method for producing rebaudioside D and rebaudioside M by reaction of glycosyltransferase, and to provide a composition for producing rebaudioside D and rebaudioside M containing glycosyltransferase.SOLUTION: Provided is a method for preparing rebaudioside D, the method including preparing rebaudioside D by reacting glucose-linked nucleotide diphosphate with rebaudioside A in the presence of glycosyltransferase B (UGT-B), wherein the glycosyltransferase B is at least one protein selected from the group consisting of proteins consisting of an amino acid sequence represented by a specific sequence.SELECTED DRAWING: Figure 1

The invention relates to the technical field of biological small molecule fluorescence detection, and discloses an amino-bridged tetraphenylethylene phenylboronic acid ester compound, a synthesis method and application thereof and a glucose fluorescence detection method. Tetraphenylethylene is used as an aggregation-induced emission mother nucleus of the compound, a quaternized amino-bridged biphenylboronic acid recognition group is used, and the compound can be used for detecting glucose. The probe can be specifically combined with glucose to generate an aggregation-induced emission effect; the invention also provides a two-step synthesis method of the compound, the synthesis route is simple, the conditions are mild, and the product purity and yield are stable; the glucose detection method constructed based on the compound avoids inherent defects of traditional enzyme method detection, improves water solubility of sensing molecules and glucose binding dynamics performance, has the advantages of being high in detection sensitivity, wide in linear range, easy and convenient to operate and high in anti-interference capacity, and is suitable for rapid quantitative detection of glucose in a water phase system.

Provided are methods for treating a disorder characterized by elevated blood glucose levels in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a hyperimmunized egg product obtained from an egg-producing animal, wherein the hyperimmunized egg product comprises a therapeutically effective amount of one or more IgY antibodies to an extracellular glucose binding domain of a sodium-glucose co-transporter SGLT. Also provided are hyperimmunized eggs and egg products produced by an animal that has been hyperimmunized with an antigen that contains an extracellular glucose binding domain of a sodium-glucose co-transporter SGLT or fraction thereof, and use of the hyperimmunized eggs or hyperimmunized egg products to reduce blood glucose levels in a subject, such as to treat type 2 diabetes. Methods or preparing the hyperimmunized eggs and hyperimmunized egg products are also provided.

This invention discloses a method for producing resistant dextrin using starch branching enzyme and α-amylase. The specific steps include: preparing pyrodextrin from corn starch via acid-thermal catalysis; then, purifying and resynthesizing the branched chains using starch branching enzyme and α-amylase; further purifying by fermentation with Saccharomyces cerevisiae; and finally, drying to obtain the resistant dextrin product. Through examples, this invention demonstrates that the obtained resistant dextrin possesses excellent glucose binding and retention capabilities, as well as resistance to digestion (resistance component ratio reaches 86.97%), good solubility, and freeze-thaw stability, making it well-suited for applications in the beverage and other industries. This provides a new, efficient, and environmentally friendly approach for the preparation and application of resistant dextrin.