35 results about "Excitation filter" patented technology

A method for enhancing digital images during an eye surgery includes producing warm white light via a lighting source by blocking blue and green wavelengths of light using an excitation filter. The method includes illuminating a pupil and iris of the eye with the warm white light during the eye surgery and collecting digital images of target anatomy using a digital camera. Within the images, a processor digitally isolates a pupil pixel region from an iris pixel region and adjusts a characteristic of constituent pixels of one of the regions. The method may be performed by executing instructions from a computer-readable storage medium to enhance a red reflex at predetermined stages of an eye surgery.

The invention relates to a multi-channel fluorescence detection device for a qPCR instrument and the qPCR instrument, the device comprises a light source assembly and an optical channel assembly, the optical channel assembly comprises an excitation assembly and a plurality of emission assemblies, the excitation assembly and the plurality of emission assemblies are rotatably arranged in a light shield, the light shield is provided with a first light inlet hole and a first light passing hole, and the first light inlet hole is communicated with the first light passing hole. The first light inlet hole is communicated with the first light outlet hole of the light source assembly; a plurality of first light passing channels are formed in the excitation assembly, the excitation assembly is rotated to enable the different first light passing channels to communicate with the first light inlet hole and the first light passing hole correspondingly, and excitation light filters and excitation dichroscopes are arranged on the first light passing channels; a second light passing channel is formed in each transmitting assembly, the different second light passing channels are communicated with the different first light passing channels by rotating the multiple transmitting assemblies, and transmitting optical filters and light detection elements are arranged on the second light passing channels. The problem that the number of optical channels on a fluorescence detection device is small is solved.

The utility model provides a fluorescence detection device and sample detection equipment, a sample hole is used for accommodating a reaction container, a light outlet hole is arranged at the bottom of the sample hole, and two light inlet holes are arranged at the side part of the sample hole along the radial direction of the sample hole; the two excitation light paths and the two light inlet holes are arranged in a one-to-one correspondence mode, and excitation light sources, excitation light filters and excitation focus lenses are sequentially arranged on the excitation light paths; the emission light path is arranged corresponding to the light outlet hole, the two excitation light paths share one emission light path, and an emission collimating mirror, an emission light filter, an emission focusing mirror and a detector are sequentially arranged on the emission light path. According to the utility model, the two excitation light paths share one emission light path, and excitation and emission only occupy one bottom surface and two side surfaces of the consumable seat, so that the fluorescence detection device is small, compact and practical; no moving or moving part exists in a light path, fluorescence detection can be achieved without a dichroscope, and reliability is high.

The utility model discloses a multicolor excitation fluorescence microscope, and belongs to the field of microscopes. Comprising a first numerical control light source; the carrying seat is arranged below the first numerical control light source; the opening is formed in the carrying plate; the carrying plate is arranged on the carrying seat; the numerical control objective lens converter is arranged below the loading plate, and the numerical control objective lens converter corresponds to the opening; the chroscope box is connected to the interface of the numerical control objective lens converter; the dichroscope is movably arranged in the dichroscope box; the X-ray plate box is arranged at the bottom of the color mirror box; the movable sheet is arranged on the X-ray plate box in a sliding manner; the excitation optical filter is movably arranged on the moving sheet; the second numerical control light source is arranged at the bottom of the light sheet box and corresponds to the excitation light filter; and the high-definition automatic focusing camera is connected with the interface of the chroscope box. The beneficial effect of the utility model is that the multicolor excitation fluorescence microscope is provided.

The invention discloses a fluorescence microscopic imaging device based on miniaturization. In the miniaturized fluorescence microscopic imaging device, light emitted by a monochromatic LED light source is changed into parallel light after passing through a hemispherical lens, and then the parallel light is incident to a gradient refractive index lens through an excitation filter and a dichroscope to be converged on a sample. After the reflected excitation fluorescence signal light passes through the dichroscope and the emission filter, a monochromatic signal is focused on the memory, and the imaging process is completed. The miniaturized fluorescence microscopic imaging system is designed by combining a series of miniaturized optical elements and memories through the characteristics of small volume and high imaging quality of the gradient refractive index lens, the quality and the cost of the system are greatly reduced, the miniaturized blood cell counting device is provided by combining a micro-fluidic chip, and the application prospect is wide. The method is expected to be widely applied to remote and hard areas such as villages, and the village medical quality is improved.

The invention relates to an assembly (12) for a converter (13) of a drive unit (10), and to a converter (13). The assembly (12) comprises a control and evaluation unit (38) and an excitation circuit (34). The excitation circuit (34) comprises an asymmetrical half-bridge (48), which has at least a first power switch (54A) and a second power switch (54B). The excitation circuit (34) has a first excitation line (60A) and a second excitation line (60B) for the drive unit (10). The first power switch (54A) is associated with the first excitation line (60A), and the second power switch (54B) is associated with the second excitation line (60B). The excitation circuit (34) has an excitation filter (62), which has a first RC element (70A) coupled to the first excitation line (60A) and a second RC element (70B) coupled to the second excitation line (60B). The control and evaluation unit (38) is connected to the RC elements (70A, 70B) in such a way that a switch-on transient and / or a switch-off transient of the power switch (54A, 54B) associated with the respective excitation line (60A, 60B) can be tapped. The control and evaluation unit (38) is designed to detect a fault state on the basis of the tapped switch-on transient and / or switch-off transient.

The application discloses a non-invasive multi-channel multi-parameter biological signal detection device and a use method thereof, relates to the technical field of biological sample signal collection, and comprises the following parts: a plurality of filter plates with different wave bands are uniformly arranged on an excitation filter plate switching device and a signal collection filter plate switching device, and a light source is arranged at one end of the excitation filter plate switching device; a detector is arranged at the light source of one end of the signal collection filter plate switching device; an excitation optical fiber bundle and a signal collection optical fiber bundle are arranged in opposite intervals; a plurality of detection heads are arranged between the excitation optical fiber bundle and the signal collection optical fiber bundle, the detection heads are arranged at one end of the excitation optical fiber bundle away from the excitation filter plate switching device, and a plurality of groups of the detection heads are arranged in the direction of a multi-well plate. The non-invasive multi-channel multi-parameter biological signal detection device and the use method thereof can solve the problems of low flux, low efficiency and single type of detected signals in the current biological signal detection field.

A portable test device includes a housing having an integrated touch screen display and a container in which a sample reservoir containing a mixture of biological samples and reagents can be placed. The portable test device also includes an optical assembly disposed in the housing, an electronic assembly configured to receive data from the optical assembly and transmit it for display on the touch screen display, and a power source in the housing to power the portable test device. The optical assembly includes an excitation filter extending across the entire optical assembly and an emission filter extending across the entire optical assembly.

The invention provides a novel efficient compact immunofluorescence light path design scheme, and aims to solve the problems of large light path loss, complex structure and high cost in the prior art. The scheme comprises an LED light source, an excitation optical filter, a dichroscope, an aspherical lens and an emission optical filter, and finally data are received and collected by a photodiode. The components are sequentially arranged on the same optical axis, so that the light path is simplified, and the cost is reduced. By optimizing the light path structure, the fluorescence detection efficiency and the signal-to-noise ratio are improved, and meanwhile, the stray light suppression technology and software correction are adopted, so that the image contrast and definition are further improved. The design has the advantages of miniaturization, high efficiency, portability and simplicity and convenience in operation, is suitable for the technical field of in-vitro diagnosis, in particular to an immunofluorescence detection technology, and has wide application prospects and market competitiveness.

The present invention discloses a miniature fluorescence microscopy imaging module, comprising an imaging lens module and an illumination device; the imaging lens module comprises an objective lens and a tube lens symmetrically arranged from the object side to the image side, an image sensor with a high spatial sampling rate arranged on the image side of the tube lens, and an emission filter arranged in front of the objective lens, or between the objective lens and the tube lens, or between the tube lens and the image sensor; the illumination device is separately arranged from the imaging lens module to form uniform illumination of the sample; the excitation light path of the illumination device is separately arranged from the detection light path of the imaging lens module. The present invention adopts an approximately symmetrical structure to design the objective lens and the tube lens, combined with a sensor with a high spatial sampling rate, to achieve high-resolution imaging at low magnification. Such a design can effectively shorten the conjugate distance, solving the problem that the image distance is still very long after the microscope is miniaturized. The three excitation illumination schemes eliminate the need to add excitation filters and dichroic mirrors, and can meet the illumination requirements of different observation samples.

This invention discloses an illumination system and an optical detection system suitable for nucleic acid detection devices. The illumination system includes a light source module, a field lens system, an excitation filter wheel, and an illumination prism module. The illumination prism module includes a beam splitter, multiple reflectors, at least two Dowell prisms, and at least two tilting reflectors. The light source module emits a beam to the field lens system for beam straightening and shaping, then filters it via the excitation filter wheel, and finally directs the beam into the beam splitter of the illumination prism module to separate it into at least two beams. The directions of the at least two beams are adjusted by the multiple reflectors, ensuring they pass through the at least two Dowell prisms and then through the at least two tilting reflectors, so that the at least two beams illuminate the sample's illumination area from different directions.

The present invention relates to an optical fiber photometric measurement technique. An optical brain signal measurement device (100) according to one embodiment may comprise a light source unit (110), an excitation filter unit (120), a first split-splice unit (130), a second split-splice unit (140), an emission filter unit (150), and a measurement unit (160). The optical brain signal measurement device 100 transmits an optical signal to a brain signal measurement object through a directional optical fiber, divides or splices the signal through a dividing and splicing part, and recognizes a wavelength range corresponding to each of the multiple signals through an optical splitter, thereby eliminating a dichroic filter. Therefore, multiple brain signals (multiple light sources) can be obtained by using the light source directivity of the optical fiber and the optical splitter.

The invention discloses a fluorescent PCR (polymerase chain reaction) detector, which belongs to the technical field of fluorescent detection and comprises a bottom plate, a reaction mechanism, a light source processing mechanism and a camera module. The reaction mechanism is composed of a temperature control assembly and a micro-fluidic chip, the micro-fluidic chip is provided with a sample adding hole for injecting a to-be-detected sample, and the temperature control assembly is used for controlling the temperature of the to-be-detected sample. The light source processing mechanism comprises a plurality of light source processing modules and a rotating motor, the light source processing modules are arranged beside the temperature control assembly, a light source, an excitation light filter, a lens, an integral rod and a two-phase color mirror are arranged in each light source processing module, and two opposite light-transmitting windows are formed in the end, close to the two-phase color mirror, of each light source processing module; the transmitting window far away from the micro-fluidic chip is provided with a transmitting optical filter, and the two-phase color mirror is positioned between the two transmitting windows and is aligned with the micro-fluidic chip. The camera module is located beside the light source processing module and used for collecting light which is emitted by the micro-fluidic chip and passes through the two-phase color mirror. The fluorescence detection precision can be improved.

The present invention discloses a terahertz near-field microscope that can be used in conjunction with fluorescence and its usage method, which relates to the field of near-field microscopes and includes: a terahertz unit, an atomic force microscope unit, a fluorescence microscope unit, and a processing unit. The terahertz unit includes: a femtosecond laser light source, an optical fiber beam splitter, an optical fiber delay line, a transmitting antenna, a first focusing parabolic mirror, a second focusing parabolic mirror, and a receiving antenna; the atomic force microscope unit includes: an atomic force probe, a quadrant laser emitter, a quadrant detector, and a sample stage; the fluorescence microscope unit includes: a high-pressure mercury lamp, an excitation filter, a first dichroic mirror, a fluorescence objective lens, an illumination light source, a collimating lens, a second dichroic mirror, an emission filter, and a fluorescence camera; the present invention utilizes the advantages of high penetration and super-resolution imaging of the terahertz near-field microscope to specifically solve the problems in measuring biological samples with a fluorescence microscope.

The utility model discloses a multichannel fluorescence microscope imaging device which comprises a rack chassis, a left side vertical plate is arranged on one side of the rack chassis, a right side vertical plate is arranged on the other side of the rack chassis, a left side upper rack is arranged on the top of the left side vertical plate, and a right side lower rack is arranged on the top of the right side vertical plate. A right upper rack is arranged at the top of the right vertical plate, an imaging darkroom is arranged between the left vertical plate and the right vertical plate, and six light filtering blocks with different wavelengths are arranged in the imaging darkroom; each light filtering block comprises an excitation light filter positioned on one side of the light filtering block, an emission light filter positioned below the light filtering block and a two-phase color mirror positioned in the middle of the light filtering block; according to the utility model, the filter wheel is controlled to rapidly switch the filter block so as to select a proper fluorescence channel, rapid, high-efficiency and high-quality imaging is finally realized, the tedious problem that an objective lens, a focal length and a filter module need to be manually adjusted in a conventional microscope is solved, and the practicability and timeliness of a multichannel fluorescence imaging system experiment are improved.

The present disclosure provides an external filter holder arrangement that comprises a pair of filter holding slots to hold two different filters such as barrier filter and excitation filter to thereby fit into any type of xenon source light microscope. The external filters holder design can be used in any type of operative microscopes by altering the dimensions of the slots and the holding mechanism. The external filter holder 100 uses various combinations of filters that can be used in the same module which aids to carry out different surgeries such as angiogram, tumour surgery, urology and thereof. Thus, the external filter holder arrangement 100 is universal, cost-effective and can be customized to various older or base variant xenon light source microscopes.

The utility model provides a fluorescence switching device for medical imaging equipment, which comprises a motor and a turntable body, the turntable body comprises a top plate, the motor is connected with the top plate, and a plurality of light filtering components are arranged in the circumferential direction of the top plate; the light filtering assembly comprises an excitation light filter support and a dichroscope support connected with the excitation light filter support, an excitation light filter is arranged on the excitation light filter support, a dichroscope is arranged on the dichroscope support, and the excitation light filter support is fixedly connected with the edge of the top plate; the excitation optical filter is located on one side of the dichroscope in the horizontal direction, and a reflection optical filter is arranged on the top plate. The structure of the light filtering assembly is greatly simplified, the size and the structural complexity of the light filtering assembly are greatly reduced, the weight of the light filtering assembly is effectively reduced, more light filtering modules can be installed on one rotary table body, and more than ten light filtering assemblies can be installed on one rotary table body at the same time. And the switching requirement of a large number of light filtering assemblies can be effectively met.

The invention relates to an assembly (12) for a converter (13) of a drive unit (10) and a converter (13). The assembly (12) comprises a control and evaluation unit (38) and an excitation circuit (34). The excitation circuit (34) comprises an asymmetrical half-bridge (48) with at least one first circuit breaker (54A) and a second circuit breaker (54B). The excitation circuit (34) has a first excitation line (60A) and a second excitation line (60B) for the drive unit (10). The first circuit breaker (54A) is assigned to the first excitation line (60A) and the second circuit breaker (54B) is assigned to the second excitation line (60B). The excitation circuit (34) has an excitation filter (62) which has a first RC element (70A) coupled to the first excitation line (60A) and a second RC element (70B) coupled to the second excitation line (60B).The control and evaluation unit (38) is connected to the RC elements (70A, 70B) such that a switch-on transient and / or a switch-off transient of the circuit breaker (54A, 54B) assigned to the respective excitation line (60A, 60B) can be tapped. The control and evaluation unit (38) is configured to detect a fault condition based on the tapped switch-on transient and / or switch-off transient.

The present invention relates to a contrast field microscope capable of obtaining a bright field image and a dark field image without an additional light source and simultaneously obtaining a fluorescence image without a dichroic mirror, and comprises: a light source; a ring-forming optical system for converting light irradiated from the light source, into ring-shaped parallel light; an excitation filter for exciting the light converted by the ring-forming optical system; a through-hole mirror for reflecting the light transmitted through the excitation filter, to an object, and passing the light reflected from the object, through a through-hole; and a reflection type objective lens forming, on the object, a focal point of the light reflected from the through-hole mirror and irradiated toward the object; and a camera for obtaining an image of the object by receiving the light reflected from the object and passing through the through-hole of the through-hole mirror.

The utility model provides a light path structure applied to a fluorescence immunoassay analyzer, which comprises an excitation light source, an excitation light filter, a light through hole and a detection sample which are sequentially arranged along the direction of an output light path, and the detection sample is sequentially provided with an emission light filter and a data processing system from bottom to top along the direction of an emission light path of the detection sample, the excitation light filter is used for selectively transmitting first light emitted by the excitation light source, and the wavelength of the first light is matched with the excitation wavelength of a detection sample; the emission optical filter is used for selectively transmitting second light emitted by the detection sample; the wavelength of the first light selectively penetrating through the excitation optical filter is matched with the excitation wavelength of the detection sample, so that fluorescent substances in the detection sample are excited to form second light, and the second light selectively penetrates through the emission optical filter and is finally converged into the data processing system. Therefore, optical signals irrelevant to detection results are filtered out, and the detection sensitivity of the fluorescence immunoassay analyzer is further improved.

The invention relates to the technical field of biology, in particular to an analysis device and method for evaluating invasion of tumor cells to a matrix, the analysis method comprises the steps that S1, matrigel simulating human tissue is prepared, a fluorescent probe is added into the matrigel, and the fluorescent probe can react with enzyme secreted by degradation after the matrigel is invaded to emit fluorescence; s2, matrigel is added into the invasion channel, and incubation is conducted for 30-40 min at the temperature of 36-37 DEG C; s3, inoculating the cell suspension into a cell loading pool, and carrying out static incubation at 36-37 DEG C for 2-3 hours; s4, adding a chemotactic factor into the chemotactic factor loading pool; s5, a laser source is arranged on one side of the invasion channel, an excitation optical filter is arranged between the laser source and the invasion channel, and an emission optical filter is arranged on the side, away from the excitation optical filter, of the invasion channel; and S6, acquiring a matrigel image in the invasion channel, and judging the invasion potential of the tumor cells according to fluorescence emitted by a degradation channel left in the matrigel by invasion, and by adopting the method, the invasion ability of the tumor cells can be observed by naked eyes.

The invention discloses a confocal endoscope imaging system based on structured light illumination. The system comprises a laser light source used for generating exciting light; the half-wave plate is placed on a light path of exciting light emitted by the exciting light source; the electro-optical modulator is placed on a light path after the exciting light passes through the half-wave plate; the excitation light filter is placed on a light path of the excitation light modulated by the electro-optical modulator; the dichroic mirror is placed on a light path after the exciting light passes through the exciting light filter; the first objective lens is placed on a light path after the exciting light passes through the dichroic mirror; and the confocal pinhole is placed on a light path formed after the exciting light is converged by the first objective lens. According to the confocal endoscope imaging system based on structured light illumination, the defect that an existing confocal endoscope imaging system does not have the super-resolution imaging capability is overcome, the technical problem that the resolution ratio is insufficient during microscopic imaging is solved, and industrial application of the system is facilitated.

An automated polymerase chain reaction (PCR) analyzer (110) for analyzing at least one sample (112) and a method of automated PCR analysis is disclosed. The automated PCR analyzer (110) comprises: - at least one controller (114); - at least one thermocycling apparatus (116) configured for subjecting the sample (112) to at least one temperature modulation controlled by the controller (114); and - at least one photometer (118) configured for detecting at least one optically detectable property of the sample (112), the photometer (118) comprising at least one excitation light source (120) configured for illuminating the sample (112) with excitation light (122), the photometer (118) further comprising at least one photodetector unit (124) configured for detecting light (123) emitted from the sample (112), the photometer (118) further comprising a movable filter cell (126) controllable by the controller (114), the movable filter cell (126) comprising a plurality of filter cell units (128), each filter cell unit (128) comprising at least one optical excitation filter element (130), at least one dichroic filter (132), at least one optical detection filter element (134) and at least one the aperture stop (174), wherein the photometer (118) comprises a working position for the filter cell units (128), wherein the movable filter cell (126) is configured for selectively positioning a selected filter cell unit (128) selected from the plurality of filter cell units (128) in the working position, wherein, in the working position, the selected filter cell unit (128) is positioned such that the excitation light (122) interacts with the optical excitation filter element (130), the dichroic filter (132) and the aperture stop (174) of the selected filter cell unit (128) and that the light (123) emitted from the sample (112) interacts with the dichroic filter (132), the aperture stop (174) and the optical detection filter element (134) of the selected filter cell unit (128).

A portable modular unit for inspecting in a timepiece the presence of a lubricating agent, or of an epilame, having fluorescent markers, from an excitation luminous flux of white light. The modular unit includes an optical housing forming a first module, a portable white light source (200) emitting an excitation luminous flux forming a second module, and a portable magnifying device forming a third module. The optical housing includes a first mounting interface for removably mounting the portable white light source on the optical housing, the first mounting interface being configured so that the excitation luminous flux emitted by the portable white light source is directed in the direction of the excitation filter; and a second mounting interface for removably mounting the portable magnifying device on the optical housing, the second mounting interface being configured so that the magnifying device is opposite the inspection opening.

A system for sample holder scanning is provided. The system comprises: a light source; a multi-band excitation filter configured to select light of at least two excitation bands for a fluorescent dye used in the sample holder. The system further includes: a multiband dichroic filter configured to reflect light of the at least two excitation bands; and a multi-band emission filter configured to transmit the fluorescent emission light of at least two bands from each reaction site of the sample holder. The multiband dichroic filter is further configured to transmit the fluorescent emission light of the at least two bands. The system also includes an optical sensor configured to detect the fluorescent emission light of the at least two wavebands to generate an image of the sample holder.

The invention discloses a bimodal semiconductor material detection system and method based on optical path multiplexing. The technical problem that an existing technology for detecting an object to be detected through combination of OCT and fluorescence spectrum can only be used under a single condition is solved. The system comprises an OCT imaging unit, a fluorescence imaging unit and a data processing unit, the OCT imaging unit comprises a broadband light source, an OCT spectrograph, an optical fiber coupler, a first collimator, a reference arm, a first band-pass optical filter, a spectroscope, a sample arm and a dynamic focusing lens. The fluorescence imaging unit comprises a fluorescence spectrometer, a No.2 band-pass optical filter, a fluorescence light source, a second collimator and an excitation optical filter; and the data processing unit is used for converting a received signal of the OCT spectrograph into an OCT image and simultaneously converting a received signal of the fluorescence spectrograph into a fluorescence spectrum for analyzing the structure and composition of the material.

The utility model provides a fluorescence detection device and sample detection equipment, the fluorescence detection device comprises a consumable seat, an excitation light path and an emission light path, the consumable seat is provided with a sample hole and two light guide grooves, and the sample hole of a focus lens is used for accommodating a reaction container; an excitation light source, an excitation collimating lens, an excitation light filter and an excitation focusing lens are sequentially arranged on the excitation light path; an emission collimating mirror, an emission optical filter, an emission focusing mirror and a detector are sequentially arranged on the emission optical path, and passbands of the excitation optical filter and the emission optical filter are not overlapped. The excitation light path and the emission light path only occupy the bottom surface and one side surface of the consumable seat, and other side surfaces are reserved for mounting the temperature control device, so that the fluorescence detection device is small, compact and practical in structure, the cost of the fluorescence detection device can be reduced, and the reliability and the sensitivity are higher.

The application discloses an alternating current modulation fluorescence micro device for CRISPR detection, and belongs to the field of biosensing and analysis instruments.The device comprises a circuit module, an optical detection module and a reaction unit; the main control board of the circuit module drives a PD detection plate and an LED excitation plate to perform excitation and collection of fluorescence signals; an excitation light source of the optical detection module is excited by the LED excitation plate to generate excitation light, which is filtered and corrected by an excitation filter and a convex lens, and then is reflected by a dichroic mirror into the reaction unit; a sample to be detected arranged on a microfluidic chip in the reaction unit is excited by the excitation light to generate emission light, which is transmitted through the dichroic mirror and an emission filter, and then the fluorescence signal is received by the PD detection plate, and the main control board is used for analysis and Bluetooth transmission.The application has the advantages of rapid detection and accurate quantification, and can realize multi-channel expansion, and is suitable for nucleic acid on-site rapid screening scenes based on CRISPR.

An adaptive robotic system for gene editing and cell manipulation is provided. The system (100) of the present invention comprises a multi-axis robotic assembly (102) with piezoelectrically actuated flexure-based joints arranged in a parallel kinematic architecture to enable submicron probe positioning relative to target cells. A mechanical end-effector mount (104) features force-limiting couplings to prevent cell damage and interchangeably mounts cell manipulation tools such as microinjection needles, nanopipettes, and electroporation electrode pairs. A microfluidic dispensing assembly (106) with pressure-controlled reagent channels and piezoelectrically actuated microvalves delivers volumetric delivery of genetic material with nanoliter precision. An optical imaging assembly (108) with variable focus optics, fluorescence excitation filters, and a Raman spectroscopic detector enables real-time visualization and confirmation of intracellular delivery events.

The invention discloses a rotating disc type scanning confocal system of a special etching process photoetching rotating disc design. A laser light source assembly, a micro-lens array dodging assembly, an excitation color filter switching assembly, a dichroscope switching assembly, a field diaphragm assembly, a high-speed turntable pin assembly, a fluorescence channel light splitting assembly, a relay system assembly and a ten-bit color filter rotating wheel assembly are innovatively designed, and particularly, the etching process of the high-speed turntable pin assembly is adopted. The problems that a traditional confocal microscope is high in phototoxicity, low in collection efficiency and incapable of imaging living cells are solved, and the defects in the prior art are overcome.