Laser fluorescent molecular imaging system and an instant fluorescent imaging method

Abstract

The invention provides a laser fluorescent molecular imaging system and an instant fluorescent imaging method. The system and the method comprise an image processing part and an image collecting part, wherein, in the image collecting part, a controller is connected with a camera, a lens adjusting motor, a transmission filter wheel, a laser filter wheel, a laser light source, a white lamp and electronic control translation stages in three directions, controls the lens adjusting motor to change focus length and aperture of a lens, controls the transmission filter wheel and the laser filter wheel to rotate and respectively switch a transmission filter and a laser filter to the acting position, controls opening and closing of the laser light source and the white lamp, and controls motor shafts of the electronic control translation stages in three directions to move; and the image processing part is connected with the camera in the image collecting part, receives fluorescent images and white light images, performs automatic cutting, pseudo-color addition and photon number statistic processing in the automatic cut area on fluorescent images, and overlays the processed fluorescent imagesand the white light images to obtain laser fluorescent molecular images. The system and the method can be applied in the fields of fluorescent image acquisition and analysis, medicine research and development and the like.

Description

technical field

[0001] The invention belongs to the technical field of molecular imaging, and relates to an excited fluorescence imaging system and a primary fluorescence imaging method based on optical imaging theory, computer image processing technology, mathematical modeling and other subject knowledge. Background technique

[0002] With the rapid development of genomics, proteomics and disease genomics, the diagnosis of diseases is developing from the traditional observation of disease characterization and routine biochemical experiment detection to the recognition of microscopic features at the gene and molecular levels. Technology can deeply understand the occurrence and development process of diseases from the level of genes and proteins, and can realize a holistic, continuous, non-invasive specific detection method that cannot be replaced by existing microscopic analysis. The theory and technology of biological molecular imaging in vivo will provide a new Prevention,...

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