Rapid quantitative detection method for drugs in hair

Abstract

The invention relates to a rapid quantitative detection method for drugs in hair. The rapid quantitative detection method comprises the following steps that (1) hair extraction is conducted, specifically, the drugs in the hair are fully released through mechanical grinding and bioenzyme extraction; (2) a sample is prepared from a to-be-detected object treated in the step (1), and the sample is dropwise added onto a reagent strip of a hair drug fluorescence immunochromatographic kit; and (3) the hair drug fluorescence immunochromatographic kit is detected through a dry immunofluorescence analyzer, and the content of an analyzed object in the to-be-detected sample is calculated. According to the rapid quantitative detection method, the advantages that sampling for hair detecting is easy, preservation is easy, the detecting time limit is long (from several weeks to several months), and information is comprehensive are reserved; the defects that conventional hair detection is complex in pretreatment, cumbersome operation, long in result analysis time and the like are overcome; the detecting time of the drugs in the hair is shortened from several hours and several days to fifteen to twenty minutes, the operation steps and technical requirements are greatly simplified, and thus non-professional technical personnel can also operate and use the rapid quantitative detection method.

Description

technical field [0001] The invention relates to a hair detection method, in particular to a rapid quantitative detection method for drugs in hair. Background technique [0002] The poison analysis of hair can be traced back to ancient my country at the earliest, and people already had the knowledge of using hair to judge poisoning at that time. Modern hair toxicology analysis started in Europe. In 1858, the British Hoppe published the first case in which the results of hair analysis were used for judgment in the "Forensic Practice Guide". In 1979, Baumgartner (Baumgartnr) used radioimmunoassay to measure the content of opium in hair, which revolutionized hair analysis. In the mid-1980s, mass spectrometry and chromatographic analysis methods were introduced into hair analysis, thus beginning a new era of hair analysis. Then hair analysis entered an era of comprehensive development, and various related researches were carried out. [0003] The main inspection materials for...

AI Technical Summary

Problems solved by technology

[0005] 1. The pretreatment of hair is quite cumbersome, requiring organic reagent cleaning, strong acid and strong alkali cracking, organic solvent extraction, etc.;

[0006] 2. The operation is complicated and takes a long time, at least 2 hours;

[0007] 3. Instruments and equipment are expensive, with dozens or even millions of testing equipment;

[0008] 4. The operation of the instrument is complicated and cannot be used by non-professionals;

[0009] 5. There are site environment requirements, and it needs to be in a fixed laboratory and cannot be moved

[0010] 6. Difficult to popularize and use at the grassroots level

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