33results about How to "Shorten lysis time" patented technology

The invention relates to a rapid quantitative detection method for drugs in hair. The rapid quantitative detection method comprises the following steps that (1) hair extraction is conducted, specifically, the drugs in the hair are fully released through mechanical grinding and bioenzyme extraction; (2) a sample is prepared from a to-be-detected object treated in the step (1), and the sample is dropwise added onto a reagent strip of a hair drug fluorescence immunochromatographic kit; and (3) the hair drug fluorescence immunochromatographic kit is detected through a dry immunofluorescence analyzer, and the content of an analyzed object in the to-be-detected sample is calculated. According to the rapid quantitative detection method, the advantages that sampling for hair detecting is easy, preservation is easy, the detecting time limit is long (from several weeks to several months), and information is comprehensive are reserved; the defects that conventional hair detection is complex in pretreatment, cumbersome operation, long in result analysis time and the like are overcome; the detecting time of the drugs in the hair is shortened from several hours and several days to fifteen to twenty minutes, the operation steps and technical requirements are greatly simplified, and thus non-professional technical personnel can also operate and use the rapid quantitative detection method.

The invention relates to the technical field of biology, in particular to a kit capable of being applied to high-throughput extraction of genome DNA from an animal tissue and an extraction method thereof. The kit capable of being applied to the high-throughput extraction of the genome DNA from the animal tissue comprises lysate buffer, binding buffer, a washing solution I, a washing solution II, eluant, protease K and magnetic beads, the composite effect of reagents is utilized to fully digest, split, damage cells for removing proteins to obtain high-purity DNA. Compared with a kit sold in themarket, the kit capable of being applied to the high-throughput extraction of the genome DNA from the animal tissue has the advantages that the pyrolyzing rate is quick, the extraction purity is high, the extraction volume is large, the used reagents are safe, stable and efficient, the kit is especially suitable for high-throughput automated production, so that the extraction efficiency of the genome DNA is greatly improved, the error is reduced, and the extraction is more accurate.

Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.

The invention discloses a method for accurately extracting DNA from wood targeted cells; through removal of exogenous pollution on a wood surface, in-situ detection of wood DNA, radial section of woodtissues, precise separation of the wood targeted cells enriched in DNA under an inverted microscope, cracking of wood cell walls, removal of a wood extract, DNA precipitation and adsorption, dissolution, purification, PCR amplification and other steps, DNA is extracted accurately from the wood tissues. The method aims at the wood targeted cells to carry out DNA accurate extraction, the quantity and purity of DNA extracted from the wood tissues are improved, and the interference of other types of cells of wood on DNA extraction is reduced. The method can be directly applied to study of molecular biology and provide a new technical support for identification of wood species.

The invention relates to a tobacco developing and anti-faking pelleted seed. The weight ratio of seeds, a seed coating agent and a bonding agent is 1 to 50 to 10; each tobacco developing and anti-faking pelleted seed comprises three pelleting layers, an inner layer is a nucleation granulation layer coating the seed, a middle layer is a primary pelleting layer coating the nucleation granulation layer, an outer layer is a secondary pelleting layer coating the primary pelleting layer, the weight ratio of the three pelleting layers is 3 to 3 to 4, and the three pelleting layers integrally or respectively contain the seed coating agent containing 1.0% of soluble starch and the bonding agent containing 20.0% of water soluble starch. A processing method of the pelleted seed comprises the steps of respectively spraying the seed coating agent containing 1.0% of soluble starch and the bonding agent containing 20.0% of water soluble starch to the bonding agent and the seed coating agent in a nucleation granulation process, a primary pelleting process and a secondary pelleting process, so as to prepare the pelleted seed. The pelleted seed is inspected according to a condition that a reactant of the reaction between iodine and starch is blue. The processing method of the anti-faking pelleted seed is simple and safe, the anti-faking period of validity is long, the splitting performance of the pelleted seed can be improved, and the inspection method is simple and convenient, high in speed and low in cost.

The invention discloses a plasmid vector continuous lysis device. The device comprises a reaction container and a driving assembly, wherein the reaction container comprises a first container, a secondcontainer, a third container and a fourth container; the first container is used for containing a resuspension turbid solution containing thalli and a resuspension solution; the second container is used for containing a lysis solution; the third container is used for containing a neutralization solution, and the fourth container is used for containing a lysis turbid solution containing the resuspension turbid solution and the lysis solution; the driving assembly comprises a first driving pump, a second driving pump and a third driving pump; the first driving pump is used for pumping the resuspension turbid solution in the first container into the fourth container, and the second driving pump is used for pumping the lysis solution in the second container into the fourth container; and thethird driving pump is used for pumping the lysis turbid solution in the fourth container into the third container. According to the plasmid vector continuous lysis device disclosed by the invention, the stability of thallus lysis is ensured, and the thallus lysis time is shortened.

The invention relates to degradable master batch, a plastic film comprising the same and a preparation method of the plastic film, and relates to the field of plastic processing, in particular relatesto the degradable master batch, the plastic film comprising the same and the preparation method of the plastic film. The degradable master batch can be degraded in various modes, and is high in degrading speed and high in efficiency. Raw materials of the degradable master batch contain the following components in percentage by weight: 8-12% of ferrocene, 72-76% of calcium carbonate, 4-8% of citric acid, 1-5% of a dispersant and 4-8% of a photosensitizer. The dispersant is prepared by compounding one or more of barium stearate, zinc stearate, calcium stearate, cadmium stearate, magnesium stearate and copper stearate. The degradable master batch accelerates the plastic film to be degraded multidimensionally while the degrading speed is increased, so that the degrading conditions of the plastic film are further broadened, and the degradable master batch can be degraded in various modes, and is high in degrading speed and high in efficiency.

The invention belongs to the technical field of diamond coating preparation, and particularly discloses a method for improving the diamond coating deposition efficiency of hot filament CVD (chemical vapor deposition) based on magnetic confinement. Improved hot wire CVD equipment comprises a gas source, a coating chamber and a vacuum pump which are connected in sequence, wherein a base table and hot filament electrode assemblies are arranged in the coating chamber, and magnetic enhancement assemblies are arranged in the coating chamber. Each magnetic enhancement assembly comprises magnetic enhancement electrodes and a shared magnetic control power supply, and a spiral hot filament is connected between the magnetic enhancement electrodes. In the dissociation stage, the magnetic control power supply is controlled to introduce 0.3-0.5 A alternating current into the magnetic enhancement electrodes; in the deposition stage, the magnetic control power supply is controlled to introduce 3-5A alternating current into the magnetic enhancement electrodes; and in the cooling stage, the magnetic control power supply is controlled to supply 1-3A alternating current to the magnetic enhancement electrodes. By the adoption of the scheme, charged particle clusters do spiral motion under the action of the magnetic field, the residence time of particles is prolonged, the effective collision probability between the particles and the to-be-coated tool is increased, and then the deposition rate is increased.