33results about How to "Shorten lysis time" patented technology

The present invention relates to a method for producing a DMC material, and especially relates to the method for producing the DMC material by silicone low-ring opening process through using a silicone low-ring opening technology. The method comprises the following steps delivering the low-ring material from a low ring tank to a reactor; heating the material to 65 DEG C, standing for a period to stratify and drain the lower acidic water; adding a KOH catalyst and keeping the temperature at 65-100 DEG C for carrying out D3 ring opening; sampling and observing the state of the open-loop material, adding water when the requirement is satisfied; conversing the material from the reactor to the tank, waiting for cracking, distilling to produce the DMC. The invention adopts the KOH catalyst, temperature control, water addition and other means to make D3 in low ring react ring opening as far as possible to obtain the material with a small amount of D3 and low viscosity which can be used for cracking and distilling to produce the DMC. This invention possesses high operationality, thereby production cost of DMC can be reduced, and the production capacity of DMC can be increased.

Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and / or improving the kinetics of luciferase-catalysed reactions.

The invention discloses a method for accurately extracting DNA from wood targeted cells; through removal of exogenous pollution on a wood surface, in-situ detection of wood DNA, radial section of woodtissues, precise separation of the wood targeted cells enriched in DNA under an inverted microscope, cracking of wood cell walls, removal of a wood extract, DNA precipitation and adsorption, dissolution, purification, PCR amplification and other steps, DNA is extracted accurately from the wood tissues. The method aims at the wood targeted cells to carry out DNA accurate extraction, the quantity and purity of DNA extracted from the wood tissues are improved, and the interference of other types of cells of wood on DNA extraction is reduced. The method can be directly applied to study of molecular biology and provide a new technical support for identification of wood species.

Provided is a method for tyre rubber pyrolysis, comprising the following steps: A, pretreating a tyre rubber; B, feeding the tyre rubber treated in step A into a composite pyrolysis chamber (2); C, heating a gas medium with a gas heating device (3) to a temperature of from 350°C to 700°C; D, continuously supplying the gas heated in step C to the composite pyrolysis chamber (2) and allowing the gas to act simultaneously with microwaves on the tyre rubber to conduct pyrolysis to obtain a solid product and a mixed gaseous product; E, subjecting the mixed gaseous product obtained in step D to oil-gas separation to remove a pyrolysis oil; and F, supplying part of the gases obtained in the oil-gas separation in step E to the gas heating device (3) described in step C and heating said part of the gases to a temperature of from 350°C to 700°C so as to recycle the same for reuse in the pyrolysis of step D. The method of the present invention can effectively reduce the energy consumption and time of pyrolysis and save costs.

The invention relates to the technical field of molecular biology, in particular to a kit and an extraction method applicable to high-throughput extraction of genomic DNA from animal tissues. The kit, which can be applied to high-throughput extraction of genomic DNA from animal tissues, includes lysate, binding solution, washing solution I, washing solution II, eluent, proteinase K and magnetic beads. By lysing and destroying cells to remove proteins, high-purity DNA is obtained. Compared with commercially available kits, the present invention has fast lysis speed, high extraction purity, and large extraction volume. The reagents used are safe, stable and efficient, and are especially suitable for high-throughput automated production. The extraction efficiency of genomic DNA is greatly improved, the error is reduced, and it is more accurate.

The invention discloses a method for preparing an organosilicon hybrid ring from organosilicon cracking oil residues. The method comprises the following steps: cutting the organic silicon cracking oilresidues into fine pieces, putting the fine pieces into a crude cracking kettle, and adding a KOH aqueous solution and D4; performing primary cracking under the vacuum condition to obtain a crude product; putting the crude product into a secondary cracking rearrangement kettle, and adding sulfonic acid and concentrated sulfuric acid for secondary cracking rearrangement to obtain a high-purity D5hybrid ring; cooling the high-purity D5 hybrid ring, and placing the cooled high-purity D5 hybrid ring into a settling tank for washing with water, precipitating, filtering and packaging. The crackedoil residues are recycled, and waste is changed into treasure; content of D5 in the hybrid ring prepared with the method is higher than 70%, production efficiency is high, and the hybrid ring is usedas a basic raw material for production in high-end printing and dyeing industries and synthesis of silicone oil for cosmetics.

The invention discloses a method of performing electrochemical strengthening liquid phase catalysis and low-temperature splitting decomposition on biomass. According to the method, ionic liquid is taken as a catalytic reaction medium in a biomass splitting decomposition process, so that the biomass is subjected to electrochemical stimulation in the ionic liquid to make a splitting decomposition reaction and generate a high-added-value split product. An electricity-assisted splitting decomposition reactor used in the method comprises a barrel, a screw type anode and a grid cylinder type cathode, wherein the screw type anode is arranged in the grid cylinder type cathode and is driven to rotate by a motor arranged on the barrel, the screw type anode and the grid cylinder type cathode are arranged in the barrel, the upper end of the barrel is provided with a feeding hole and an air outlet, and the lower part of the barrel is provided with a discharge hole, an ionic liquid inlet and an air inlet. The biomass is heated evenly, the chemical bond of lignocellulose is enabled to break, and an ionic pair midbody is generated and is subjected to electrical field stimulation, thus increasing the splitting decomposition rate; the efficiency is high, the process is easy to control, and the use ratio of the biomass is improved.

The invention provides a solid organic material cracking method and system. The method comprises the following steps of: A, pre-treating a solid organic material; B, sending the pre-treated solid organic material into a cracking device; C, heating a gas medium to 350-700 DEG C; D, continuously importing the heated gas medium into the cracking device to act on the solid organic material with microwave, so as to carry out cracking to obtain a solid product and a mixed gaseous product; and E, importing a part of the obtained mixed gaseous product into a gas heating device in the step C so as to heat the mixed gaseous product to 350-700 DEG C to be circularly used for the cracking in the step D. The process can effectively decrease the cracking energy consumption and the cracking time, and can save the cost.

The invention relates to a derivative of a composition extracted from animal organs, wherein the animal organs are selected from livers, small intestines, large intestines and lungs, and animals are selected from cows, pigs, sheep and dogs.