This invention belongs to the field of
biomedical technology, specifically relating to a dissociation solution for aluminum
adjuvant-adsorbed influenza vaccines and a method for determining
antigen content. The dissociation solution of this invention is composed of
potassium phosphate solution,
diethanolamine, Triton X-100, Tween-20, dodecyl dimethyl
betaine, and water. This dissociation solution exhibits excellent dissociation efficiency, achieving a
recovery rate of 106%-110% for
hemagglutinin from H1N1, H3N2, and BV influenza vaccines. It effectively protects
antigen activity and is simple to operate with wide applicability. The dissociated sample can be directly used for mainstream detection methods such as one-way immunodiffusion
assay,
enzyme-linked immunosorbent
assay, and high-performance liquid
chromatography, enabling precise quantification of
antigen content in aluminum
adjuvant-adsorbed influenza vaccines and providing reliable
technical support for vaccine
quality control.