The invention relates to a releasing agent for detecting the
folic acid content of a
human body and application of the releasing agent, namely a treating fluid R1 is a
mixed solution of
sodium salicylate,
guanidine isothiocyanate and
tris (2-formylethyl)
phosphine hydrochloride, effective denaturation dissociation can be carried out on
protein within the pH value range of 5.0-7.0, the
mixed solution can be stably stored, mild, efficient and sufficient dissociation is realized, and the content of
folic acid in the
human body is detected. The
folic acid detection sensitivity is improved. The releasing agent is used in a corresponding immunodetection method so as to solve the technical problems that an existing product is complex in process or incomplete in releasing, poor in sensitivity and
repeatability, unstable in treating fluid and unsatisfactory in sample comparison correlation. Through magnetic particle
chemiluminescence detection, folic acid and a corresponding detection
reagent and a detection method, the sensitivity and
repeatability of the
reagent and the comparison correlation of samples are better improved, the effect is ideal, and the performance reaches the level of international products. The process is more simplified, large-batch samples can be rapidly detected at high
throughput, and clinical application is facilitated.