Coal gangue microbial fungal fertilizer and preparation method thereof

A production method, coal gangue technology, applied in the direction of microbial treatment, fertilizer mixture, bio-organic part treatment, etc., can solve the problems of coal gangue not being effectively used, low carbon content, bulging, etc., and improve the ability to absorb and utilize phosphorus , Improve phosphorus absorption coefficient, simple and reliable process

Active Publication Date: 2022-01-28
中国烟草总公司贵州省公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When coal gangue is used as fuel, the carbon content is too low, resulting in low efficiency; when used as building materials, due to the oxidation of sulfur and organic matter in coal gangue, it will cause bulging and pulverization, and sulfur, phosphorus, nitrogen and other components will also affect Strength and quality of coal gangue products
Therefore, coal gangue has not been effectively utilized so far

Method used

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  • Coal gangue microbial fungal fertilizer and preparation method thereof
  • Coal gangue microbial fungal fertilizer and preparation method thereof
  • Coal gangue microbial fungal fertilizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Isolation and screening of functional strains

[0038] 1) Collection of samples

[0039] (1) The samples were collected from coal gangue hills and nearby soil in a coal production area in Guizhou. Select the root soil of plants with good growth, first shovel off the surface soil, dig 10-20cm along the root of the plant, and collect the soil in a sterile ziplock bag by "shaking the root method".

[0040] (2) The ice box is sealed and taken back to the laboratory. After removing the plant residues, crush them through a 20-mesh sieve.

[0041] (3) Store the samples in a refrigerator at 4°C for refrigerated storage.

[0042] 2) Screening of functional strains

[0043] (1) Accurately weigh 1 g of the sample into a sterilized 250 mL Erlenmeyer flask, and add 99 mL of sterile water.

[0044] (2) Shake for 30 minutes in a constant temperature shaker at 170r / min and around 28°C.

[0045] (3) After shaking for 30 minutes, take the supernatant and dilute to 1.0×10 ...

Embodiment 2

[0052] Embodiment 2: the drawing of the growth curve of functional bacterial strain

[0053] 1) Preparation of fungal spore suspension: use an inoculation needle to pick the mycelia of the fungus to be tested on the PDA slant medium, activate at 30°C for 36-72 hours, take out after a large number of fungal spores grow, and use 5mL sterile distilled water on the ultra-clean workbench Rinse the slant, make fungal spore suspension, dilute to 100mL, shake well and set aside.

[0054] 2) Inoculation and cultivation: Pour 100mL LB liquid medium into a 250mL Erlenmeyer flask, prepare a total of 30 bottles, put them into an autoclave for sterilization at 121°C for 30min, and inoculate the prepared fungal spore suspension at 5% Add the amount into the sterilized LB liquid medium, 170r·min -1 , 30 ℃ shaker cultivation. Mark the bacteria number and culture time on the Erlenmeyer flask.

[0055] 3) Determination: Take out 3 bottles every 24 hours, dry the blank filter paper at 105°C to...

Embodiment 3

[0057] Embodiment 3: the identification of functional bacterial strain

[0058] In the present invention, the fungus GZT-Asp01 entrusts China Center for Type Culture Collection to identify it.

[0059] 1) Colony appearance

[0060] The primary mycelium of GZT-Asp01 fungus is white, hairy, protruding in the middle of the colony, and spreads radially. After 5 days, smoke green spores begin to appear in the middle of the colony. After about 9 days, the mycelium can cover the entire plate, and the culture can be seen from the back of the plate. The basal matrix turns yellowish brown, such as figure 2 shown.

[0061] 2) Microscopic morphology of fungi

[0062] The GZT-Asp01 fungus was stained with safranin, and the microscopic morphology of the fungus was observed under an optical microscope after staining. It can be observed with an optical microscope that the hyphae of the GZT-Asp01 fungus are transparent, and the top of the conidiophores swells into an apical capsule, which...

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Abstract

The invention discloses a coal gangue microbial fungal fertilizer and a preparation method thereof. The fertilizer comprises or is a product obtained by treating raw materials with aspergillus fumigatus GZT-Asp01; the main component of the raw materials is coal gangue or coal gangue; the method for treating the raw materials by using the aspergillus fumigatus GZT-Asp01 comprises the following steps: crushing the raw materials, and preparing the aspergillus fumigatus GZT-Asp01 into a bacterial solution; and under continuous stirring, spraying the bacterial liquid on the crushed raw materials, performing uniform stirring, timed overturning, temperature control treatment and airing to obtain a product. According to the coal gangue microbial fungal fertilizer disclosed by the invention, the coal gangue is treated by using the aspergillus fumigatus GZT-Asp01, so that insoluble phosphorus, potassium, calcium, nitrogen and other components in the coal gangue can be effectively converted into available phosphorus, quick-acting potassium, exchangeable calcium, hydrolytic nitrogen and other nutritional components which can be absorbed by plants, and resource reutilization of the coal gangue is realized; in addition, the technological process for dissociating the coal gangue through the aspergillus fumigatus GZT-Asp01 is remarkable in effect, low in cost and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of microbial mineral fertilizers, in particular to a coal gangue microbial fungal fertilizer and a preparation method thereof. Background technique [0002] my country is a large country of coal production, and coal occupies an important position in my country's energy structure. The solid waste generated during the coal mining and washing process is also one of the industrial wastes with the largest accumulation in my country. At present, the main disposal method of coal gangue is open-air accumulation, and a large amount of coal gangue stacking will cause a series of hazards: (1) a large amount of coal gangue stacking occupies land resources, and there are safety hazards such as dam failure; (2) weathering and spontaneous combustion of coal gangue will Generate dust, emit CO, CO 2 , SO 2 、H 2 S. NO X (3) After the coal gangue is washed by rainwater, its components will be gradually dissolved and flow ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05D9/00C05F17/20C05G1/00
CPCC05D9/00C05F17/20C05B17/00Y02W30/40
Inventor 谢承卫吉俐李小军盛定红杜鑫
Owner 中国烟草总公司贵州省公司
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