3results about How to "Effective dissociation" patented technology

The application belongs to the technical field of natural substance separation and extraction, and particularly relates to a kind of Haematococcus pluvialis multi-product cascade separation method based on eutectic solvent sequential extraction and enzyme membrane reactor coupling, comprising the following steps: (1) cell wall weakening pretreatment step; (2) water-soluble component extraction step; (3) pigment component extraction step; (4) astaxanthin extraction step; (5) enzyme conversion step. Through the synergistic effect of eutectic solvent sequential extraction and enzyme membrane reactor, efficient, green and continuous cascade separation of water-soluble components, pigments, astaxanthin and xylo-oligosaccharides in Haematococcus pluvialis is realized, and product purity and resource utilization are improved.

This invention relates to a kit for the quantitative detection of IGF-1 and its preparation and detection method. The kit includes calibrators, quality control materials, internal standard solution, immunoenrichment solution, dissociation solution, elution solution, mobile phase 1, and mobile phase 2. This invention enables fully automated top-down detection of intact IGF-1 protein, significantly improving sample processing efficiency. The matrix solution used in the kit preparation employs human serum with endogenous IGF-1 removed, maximally mimicking the in vivo environment and reducing detection bias caused by different matrix components. The elution buffer uses a formic acid-hydrochloric acid mixed acid-acetonitrile system. This system provides a mild ionization environment through formic acid, and the synergistic effect of hydrochloric acid in stabilizing the solution pH reduces ion inhibition effects, improves the IGF-1 response signal, and thus enhances the signal-to-noise ratio of the detection.

This invention discloses a two-dimensional porous carbon material, its preparation method, and its application, belonging to the field of water electrolysis for hydrogen production technology. The preparation method of the two-dimensional porous carbon material is as follows: a polyacrylamide solution, a reducing agent, a sulfur-containing compound, and a metal salt are stirred to form a gel-like spatial network structure, followed by a microwave hydrothermal reaction to obtain a precursor; the precursor is dissolved in a water-soluble salt to obtain a mixed solution, which is then freeze-dried to obtain a mixture; the mixture is annealed, and after annealing, it is washed with water to obtain the two-dimensional porous carbon material. The two-dimensional porous carbon material prepared by this invention possesses excellent conductivity and a continuous electron transport path, providing a sufficient electrode / electrolyte interface for the electrocatalytic hydrogen evolution reaction and improving the electrocatalytic efficiency of sulfides.