Estradiol release reagent and detection kit thereof

A detection kit, estradiol technology, applied in biological testing, measuring devices, material inspection products, etc., to achieve the effect of improving detection efficiency

Pending Publication Date: 2021-02-23
深圳市辰纳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing estradiol-releasing agents are difficult to sufficiently

Method used

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  • Estradiol release reagent and detection kit thereof
  • Estradiol release reagent and detection kit thereof
  • Estradiol release reagent and detection kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0042]In one embodiment, the method of preparing the estradiol release reagent comprises: taking the desired amount of the estradiol release agent original component, and dissolving each of the original components with solvents such as DMSO, DMF or pure water, formulated The 500 mg / ml of mother liquor was diluted with buffer to the corresponding concentration, and the estradiol release reagent was obtained.

[0043]In one embodiment, the release agent is a component portion of the estradiol enzymatic chemiluminescence immunoassay compared to the prior art, and the estradiol can be quickly and effectively condensed from albumin and sex hormone binding protein. It is removed, the sensitivity and specificity of the estradiol immunoassay reagent have been significantly improved.

[0044]In a second aspect, in one embodiment, a test kit is provided, and the estradiol release reagent is contained in the first aspect.

[0045]In one embodiment, the detection kit is an estradiol nanomagnetic parti...

Example

[0084]Example 1 ~ 4

[0085]1. Sample release agent 1: contains 100 μg / ml of Narzole, 2 mg / ml of sodium trichloroacetate, 50 mM MES buffer, pH 6.0.

[0086]2, sample release agent 2: containing 200 μg / ml of dihydroformosterone, 2 mg / ml of talchloroacetate, 100 mM MOPSO buffer, pH 6.5.

[0087]3. Sample release agent 3: containing 0.05 mg / ml cortisol, 0.1 mg / ml permeable testosterone, 6 mg / ml 8-aniline-1-naphthalenesulfonic acid (ANS), 60 mM Tris-HCl buffer, and pH is 8.5.

[0088]4. Sample release agent 4: containing 50 μg / ml of Narzole, 200 μg / ml 3,4-diaphragonflial tetrahydrofuran (DVT), 5 mg / ml8-aniline-1-naphthalenesulfonic acid (ANS), 50 mM PBS buffer, pH 7.4.

[0089]In the present embodiment, the method of preparing an estradiol magnetic separation reagent is as follows: Take the commercially available streptavidin magnetic particles, diluted with 0.05 mol / L of Tris-HCl buffer to 0.01% by weight final concentration, ie A magnetic separation reagent of estradiol.

[0090]I...

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Abstract

According to the estradiol release reagent and the detection kit thereof, the estradiol release reagent can influence the combination of estradiol and sex hormone binding protein, so that estradiol isreleased; the estradiol releasing reagent contains at least one of a reagent for competitively binding to the sex hormone binding protein and a reagent for reducing the binding force between estradiol and the sex hormone binding protein. Compared with the prior art, the estradiol releasing agent is used as a component of an estradiol enzymatic chemiluminescence immunoassay reagent, and estradiolcan be rapidly and effectively dissociated from albumin and sex hormone binding protein.

Description

technical field [0001] The invention relates to the technical field of steroid estrogen release, in particular to an estradiol release reagent and a detection kit thereof. Background technique [0002] Estradiol is a steroid estrogen derived from cholesterol in humans and higher animals. Cholesterol is oxidized to generate pregnenolone, and then from pregnenolone to progesterone, 17α-hydroxyprogesterone, and finally to the intermediate androstenedione. Part of androstenedione is converted to testosterone, which is converted to estradiol by aromatase, and the other part is aromatized to estrone, which is then converted to estradiol. Estradiol, as a major female hormone, is responsible for the regulation of female characteristics, maturation of accessory sex organs, and menstrual-ovulatory cycle. Granulosa cells, placenta, theca cells, Leydig cells and adrenal cortex cells all produce estradiol. In women of childbearing age, estradiol is primarily produced by the metabolism...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/543G01N33/53
CPCG01N33/743G01N33/54326G01N33/5306
Inventor 谢钧于琴丁军发汪紫晶
Owner 深圳市辰纳生物科技有限公司
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