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Estradiol release reagent and detection kit thereof

A detection kit, estradiol technology, applied in biological testing, measuring devices, material inspection products, etc., to achieve the effect of improving detection efficiency

Pending Publication Date: 2021-02-23
深圳市辰纳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing estradiol-releasing agents are difficult to sufficiently dissociate estradiol from albumin and sex hormone-binding protein

Method used

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  • Estradiol release reagent and detection kit thereof
  • Estradiol release reagent and detection kit thereof
  • Estradiol release reagent and detection kit thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0042] In one embodiment, the preparation method of the estradiol-releasing agent comprises: taking the required amount of original components of the estradiol-releasing agent, and dissolving each original component with a solvent such as DMSO, DMF or pure water, and preparing into 500 mg / mL stock solution, and diluted to the corresponding concentration with buffer solution to prepare the estradiol releasing reagent.

[0043] In one embodiment, compared with the prior art, the release agent is used as a component of estradiol enzymatic chemiluminescent immunoassay reagent, which can rapidly and effectively decompose estradiol from albumin and sex hormone binding protein The sensitivity and specificity of the estradiol immunoassay reagent are significantly improved.

[0044] In the second aspect, in one embodiment, a detection kit is provided, which contains the estradiol releasing reagent described in the first aspect.

[0045] In one embodiment, the detection kit is an estra...

Embodiment 1～4

[0085] 1. Sample release agent 1: Contains 100 μg / mL danazol, 2 mg / mL sodium trichloroacetate, 50 mM MES buffer, pH 6.0.

[0086] 2. Sample release agent 2: containing 200 μg / mL dihydrotestosterone, 2 mg / mL sodium trichloroacetate, 100 mM MOPSO buffer, pH 6.5.

[0087] 3. Sample release agent 3: Contains 0.05 mg / mL cortisol, 0.1 mg / mL mesterolone, 6 mg / mL 8-aniline-1-naphthalenesulfonic acid (ANS), 60 mM Tris-HCl buffer solution, adjust the pH to 8.5.

[0088] 4. Sample release agent 4: containing 50 μg / mL danazol, 200 μg / mL 3,4-divanillyl tetrahydrofuran (DVT), 5 mg / mL 8-aniline-1-naphthalenesulfonic acid (ANS), 50 mM PBS buffer, pH7.4.

[0089] In this example, the preparation method of the estradiol magnetic separation reagent is as follows: take the commercially available streptavidin magnetic particles, dilute it to a final concentration of 0.01wt% with 0.05mol / L Tris-HCl buffer solution, and prepare Magnetic separation reagent for estradiol.

[0090] In this example, ...

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Abstract

According to the estradiol release reagent and the detection kit thereof, the estradiol release reagent can influence the combination of estradiol and sex hormone binding protein, so that estradiol isreleased; the estradiol releasing reagent contains at least one of a reagent for competitively binding to the sex hormone binding protein and a reagent for reducing the binding force between estradiol and the sex hormone binding protein. Compared with the prior art, the estradiol releasing agent is used as a component of an estradiol enzymatic chemiluminescence immunoassay reagent, and estradiolcan be rapidly and effectively dissociated from albumin and sex hormone binding protein.

Description

technical field [0001] The invention relates to the technical field of steroid estrogen release, in particular to an estradiol release reagent and a detection kit thereof. Background technique [0002] Estradiol is a steroid estrogen derived from cholesterol in humans and higher animals. Cholesterol is oxidized to generate pregnenolone, and then from pregnenolone to progesterone, 17α-hydroxyprogesterone, and finally to the intermediate androstenedione. Part of androstenedione is converted to testosterone, which is converted to estradiol by aromatase, and the other part is aromatized to estrone, which is then converted to estradiol. Estradiol, as a major female hormone, is responsible for the regulation of female characteristics, maturation of accessory sex organs, and menstrual-ovulatory cycle. Granulosa cells, placenta, theca cells, Leydig cells and adrenal cortex cells all produce estradiol. In women of childbearing age, estradiol is primarily produced by the metabolism...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/543G01N33/53
CPCG01N33/743G01N33/54326G01N33/5306
Inventor 谢钧于琴丁军发汪紫晶
Owner 深圳市辰纳生物科技有限公司
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