63results about How to "Fast dissociation" patented technology

The invention relates to a kit for extracting blood genome DNA based on a magnetic bead method. The kit comprises a cell lysis solution, a proteinase K solution, a magnetic bead dispersion solution, a first cleaning solution, a second cleaning solution and an elution solution, wherein the cell lysis solution comprises a guanidine compound, sodium chloride and a tween 20; a magnetic bead in the magnetic bead dispersion solution has the characteristics that a core is superparamagnetic ferroferric oxide, the surface is coated with silicon hydroxyl, and the particle size is 200-800nm; the first cleaning solution comprises Tris alkali, EDTA, isopropanol and water; the second cleaning solution is an ethanol solution; and the elution solution is Tris alkali or de-ionized water. The kit is simple to operate and can greatly improve working efficiency of blood genome DNA extraction; without pretreating erythrocytes, the cell lysis solution is directly adopted for pyrolyzing blood cells, and the magnetic bead dispersion solution can be added and combined with the genome DNA; and the kit is high in extraction volume and suitable for high-throughput experiments.

The invention relates to a preparation method and product of a chitosan composite flocculant with the advantages that organic contaminants in water can be effectively removed; heavy metal ions such as nickel, chromium and lead in water can be adsorbed; the adsorption quantity on heavy metal ions is great; the adsorption efficiency is high. The product is prepared by using chitosan as raw materials, using glutaraldehyde as a cross-linking agent and using attapulgite subjected to water washing and acid washing treatment and tannin modification as a modifying agent. The adsorption removal rate of the composite flocculant product on the heavy metal ions such as low-concentration nickel, chromium, lead and mercury reaches 90 percent or higher. The preparation method of the composite flocculant for adsorbing heavy metal ions has the characteristics that the process is simple; the cost is low; special equipment is not needed; the industrial implementation is easy, and the like. The consumption of chitosan by the composite flocculant is reduced; the cost is reduced.

The invention discloses a Material for thioketal bonding doxorubicin and polyphosphate and a preparation method and application thereof. The method comprises the following steps: (1) using benzyl alcohol as an initiator, ring-opening polymerization of PPEG and phosphate ester monomer to obtain P (PPEG co AEP). (2) P (PPEG-Co-AEP (Cya)) was obtained by clicking on P (PPEG-Co-AEP) with mercaptoethylamine. (3) adding activated dipropionic acid containing thioketal bond into adriamycin for amide reaction. (4) P (PPEG-Co-AEP (Cya)) was synthesized by amidation reaction to give the material containing thioketal bonded doxorubicin and polyphosphate. The nanoparticles were self-assembled with photosensitizer, and the drug was rapidly released under 660 nm near-infrared light, the drug concentration and active oxygen content in tumor site increased, and the therapeutic effect of chemotherapy and PDT was improved. The nanoparticles have potential clinical application value.

The invention provides a dissociation method of an antigen in an aluminum adjuvant absorption type novel coronavirus inactivated vaccine. A desorption agent composition adopted by the method comprisescane sugar, magnesium chloride, potassium phosphate and polysorbate-80, wherein the dosage ratio of the cane sugar to the magnesium chloride to the potassium phosphate to the polysorbate-80 is (50-100)g: (2-6)g: (0.3-0.6)mol: (0.5-1)ml. Antigens in the aluminum adjuvant absorption type novel coronavirus inactivated vaccine are dissociated, a solution system can be stabilized, the immunocompetenceof the antigens can be protected, the absorption capacity of an aluminum adjuvant can be destroyed, and the antigens can be rapidly separated from the aluminum adjuvant, interference of an aluminum adjuvant on accurate quantification of the antigen is eliminated, the method can solve a problem of unstable biological structure of the new coronavirus epitope in the dissociation process, has the characteristics of good repeatability, high accuracy and strong specificity, and provides an important tool for evaluating the key quality attributes of the novel coronavirus inactivated vaccine.

The invention relates to a treating technology of smelting slag containing iron and silicon, and particularly provides a method for treating smelting slag through electrolytic reduction of a molten aluminum salt. The technological process of the method comprises the following steps that firstly, raw materials except the smelting slag are fully dried and dehydrated, the molten aluminum salt is putinto an electrolytic bath with a gas collecting cover for heating, an auxiliary material and the smelting slag are added in sequence to be mixed so as to form a melt, an electrode is inserted, a direct current is introduced to carry out reduction reaction at a certain temperature and voltage, a slag layer and a liquid metal layer can be guided out through a slag tap and liquid discharging respectively to form a product after the reaction is finished, and a molten salt medium can be recycled. The method has the advantages that the process is simple, the metal reduction is thorough, the slag isgreatly reduced and recycled, the physical sensible heat of the smelting slag can be utilized in the process, and energy conservation and environmental protection are achieved.

The invention relates to a mineral dissociation method based on fracture characteristic criteria, belongs to the technical field of mineral separation, and solves the problems of low accuracy of an existing mineral separation determination method, high cost of a mineral microscopic image determination method and long determination time. The mineral dissociation method comprises the following stepsthat ore particles are crushed and ground through a crushing system, and the crushed and ground ore particles are fed into an ore grinding dissociation system to be ground and dissociated; the ore particles ground and dissociated by the ore grinding dissociation system are fed into a grading screening system to be subjected to grading screening operation, and the ore particles smaller than screenholes in size are fed into a camera shooting system; the camera shooting system is used for carrying out image acquisition on fracture morphology characteristics of the fed ore particles and transmitting fracture morphology pictures of the ore particles to the image processing and calculating system; and the image processing and calculating system acquires the surface dissociation degree of the ore particles. The method can quickly and accurately obtain the dissociation degree of the ore particles, and is convenient to operate, low in cost and wide in application range.

The invention belongs to the technical field of electrocatalysis, and discloses a synthesis method of a hollow oxide / phosphide carbon-coated composite material for electrocatalytic hydrogen production. The synthesis method mainly comprises the following steps: synthesizing nano silicon dioxide particles; synthesizing hollow nano cerium dioxide; synthesizing H-CeO2 / Ni@NC; and synthesizing a targetproduct H-CeO2 / Ni@NC. According to the invention, a transition metal oxide is used for regulating and controlling transition metals, so that rapid water molecule dissociation and hydrogen removal reaction are realized, and efficient catalytic hydrolysis hydrogen production reaction is realized. The hollow cerium dioxide nanospheres are composed of octahedral sub-nano cerium dioxide particles, so that the hollow cerium dioxide nanospheres have the porous characteristic, and electrolyte and gas transmission is faster; the hollow structure can expose a larger specific surface area, so that the utilization rate of active sites is improved.

A method for extracting nucleic acid from animal tissue comprises the steps that 1, an animal tissue sample and a nucleic acid extracting solution are added into a bubbling bed container, the nucleic acid extracting solution comprises magnetic microspheres capable of absorbing the nucleic acid, and the bottom of the bubbling bed container is provided with a microwell plate and an air inlet; 2, nitrogen or inert gas is fed into the bubbling bed container from the air inlet to make the animal tissue sample fully mixed and reacted with the nucleic acid extracting solution, and separated nucleic acid is absorbed by the magnetic microspheres. By means of the method for extracting the nucleic acid from the animal tissue, rapid dissociation can be conducted on the animal tissue, the nucleic acid can be efficiently separated and purified, and the method for extracting the nucleic acid from the animal tissue can be widely applied to the field of animal epidemic disease detection.

The invention discloses an inflatable life-saving backpack for marine peril escape. The inflatable life-saving backpack comprises a backpack box body, a power cavity is formed in the backpack box body, an unfolding cavity located on the upper side of the power cavity is formed in the backpack box body, a composite power device is arranged in the power cavity, an auxiliary raft unfolding device which is positioned on the upper side of the composite power device and is symmetrical front and back is arranged in the unfolding cavity, auxiliary raft descending devices are correspondingly hinged toconnecting rods on the front side and the rear side, a main raft unfolding device located on the right side of the auxiliary raft unfolding device is arranged in the unfolding cavity, and an inflationdevice located on the lower side of the main raft unfolding device is arranged in the power cavity. According to the inflatable life-saving backpack, a propeller device can be used for enabling a person in distress to quickly free an accident sea area so as to be prevented from being influenced by vortex waves generated by a sunken ship; and an automatic inflation device can enable the life-saving backpack to become a small-sized life raft, so that the person in distress is prevented from being soaked in water for a long time, and then the survival rate of the person in distress in distress is effectively improved.

The invention discloses a composite biological enzyme air treatment bionic filtering element, relates to the technical field of air purifiers, and in particular to the technical field of filtering and treating of air purifier filtering elements. The composite biological enzyme air treatment bionic filtering element comprises a composite net layer, an HEPA filtering net layer and an activated carbon cotton layer, and the composite net layer, the HEPA filtering net layer and the activated carbon cotton layer are sequentially arranged; and a water washing system is arranged on one side, far away from the HEPA filtering net layer, of the activated carbon cotton layer. The composite biological enzyme air treatment bionic filtering element achieves the aims that the structure is simple, the indoor virus cross infection is avoided, the secondary pollution to indoor air can be avoided, and peculiar smells in air can be effectively and thoroughly removed.

The invention relates to the field of rice and fish cultivation, and particularly discloses a purification device and a purification method for rice field water quality in rice and fish cultivation. The purification device comprises a shell, wherein one side of the shell is slidably connected with two dovetail sliding blocks, the shell is slidably connected with an L-shaped vertical plate through the dovetail sliding blocks, the dovetail sliding blocks are fixedly connected to one side of the vertical end of the L-shaped vertical plate, a water outlet hole is formed in the vertical plate, the water outlet hole is fixedly connected to a side wall plate of the shell, a water inlet is formed in the shell and communicated with the water outlet hole, and a filter screen is fixedly connected to the end of the water inlet of the shell. According to the invention, water can be drained and purified under the condition of different water levels in the rice field, fish can be effectively caught through the device when fish eating is needed, fish and shrimps are prevented from entering the device when fish eating is not needed, and the device is very convenient to use.

The invention relates to guanidine gum fracturing fluid gel capable of reducing pH dependence, a guanidine gum fracturing fluid system and a preparation method and application thereof. The fracturing fluid gel is prepared from the following components in percentage by weight: 0.01 to 0.2 percent of nano material, 0.1 to 0.5 percent of guanidine gum, 0.1 to 0.5 percent of organic boron-titanium cross-linking agent and the balance of water. The fracturing fluid gel is more stable in network structure and good in temperature resistance and shear resistance. The invention further provides the preparation method and application of the fracturing fluid gel. According to the guanidine gum fracturing fluid system, the dependence on pH is reduced, and the damage degree of the high pH value to a reservoir is reduced.

An ore dressing technology for increasing the quality and recovery rate of ore concentrate includes such steps as crushing, rolling, grinding, and separating. Another two approaches are also disclosed.

The present invention is technological process with high ore dressing efficiency and goal. One of the technological process includes the steps of crushing, grinding and ore separating, and the crushing step has rolling crusher adopted and the fine grain tail eliminated. One other technological process includes the steps of crushing, grinding in a autogenous mill, grinding and ore separating. One third technological process includes the steps of crushing and ore separating. The present invention has raised ore grinding and ore separating efficiency, raised production capacity, and raised concentrate grade and recovering rate.

The invention relates to a multi-mode luminous carbon dot as well as a preparation method and application thereof. The preparation method of the multi-mode luminous carbon dots comprises the following steps that: fibroin, strong base and water are mixed according to a mass ratio of 1: (0.1-10): (10-500) to form a mixed solution; the mixed solution is placed in a microwave reactor to be subjected to a microwave reaction, the microwave power ranges from 100 W to 1000 W, and the reaction time ranges from 0.5 min to 10 min; and a product obtained after the microwave reaction is purified and dried.