Method for extracting nucleic acid from animal tissue

Abstract

A method for extracting nucleic acid from animal tissue comprises the steps that 1, an animal tissue sample and a nucleic acid extracting solution are added into a bubbling bed container, the nucleic acid extracting solution comprises magnetic microspheres capable of absorbing the nucleic acid, and the bottom of the bubbling bed container is provided with a microwell plate and an air inlet; 2, nitrogen or inert gas is fed into the bubbling bed container from the air inlet to make the animal tissue sample fully mixed and reacted with the nucleic acid extracting solution, and separated nucleic acid is absorbed by the magnetic microspheres. By means of the method for extracting the nucleic acid from the animal tissue, rapid dissociation can be conducted on the animal tissue, the nucleic acid can be efficiently separated and purified, and the method for extracting the nucleic acid from the animal tissue can be widely applied to the field of animal epidemic disease detection.

Description

technical field [0001] The present invention relates to a method for extracting nucleic acid from animal tissue. Background technique [0002] Animal diseases have an important impact on animal husbandry and human food safety. Using molecular biology methods to diagnose pathogens in animal tissues can obtain the most accurate results. A critical step is to extract bacterial or viral DNA from animal tissue samples. The ability to obtain different kinds of bacterial DNA or different kinds of virus DNA, as well as the integrity, purity and concentration of the genome, is the primary goal of extracting DNA from animal tissues for the diagnosis of animal pathogens. [0003] The existing DNA extraction process is often polluted, the steps are cumbersome, and the extraction process takes a long time. Extraction methods such as sodium perchlorate method, SDS method and urea method, but these methods require a large sample size, and organic substances such as phenols are easy to re...

AI Technical Summary

Problems solved by technology

The added acidic substance (HCl) will react with the ferroferric oxide of the magnetic core to a certain extent, which will have an uncertain effect on the magnetic properties of the magnetic microspheres, resulting in unstable product quality.

[0006] In addition, magnetic microspheres are prone to agglomeration and precipitation in the nucleic acid extraction solution, which seriously affects the extraction efficiency of nucleic acid

In the prior art, mechanical stirring or ultrasonic oscillation is usually used to overcome this problem, but mechanical stirring or ultrasonic oscillation will cause certain damage to the structure and adsorption capacity of magnetic microspheres.

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