Desorption composition of adsorption type vaccine containing CpG ODN and application of desorption composition
A technology of desorption and composition, applied in the field of desorption composition, can solve the problems of unreported CpGODN compound adjuvant absorption-adsorption type vaccine desorption method, inapplicability of vaccine, long time consumption, etc., to achieve protection of antigen immune activity, Easy to promote and apply, fast separation effect
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Embodiment 1
[0063] This implementation provides a method for detecting antigen efficacy in a CpG ODN composite adjuvant adsorption vaccine, the specific steps are as follows:
[0064] Step 1: Preparation of desorption reagents: wherein the final molar concentration of potassium phosphate is 0.6M, the final molar concentration of magnesium chloride is 0.1M, the final volume concentration of Triton-100 is 1%, and the final volume concentration of M199 medium is 20% ;
[0065] Step 2: Desorb the CpG ODN composite adjuvant-adsorbed vaccine reference substance and the sample to be tested: Mix the reference substance and sample with the desorption reagent in a volume ratio of 1:1, incubate at 28°C for 60 minutes, and incubate every 20 minutes during the incubation process. Invert and mix once;
[0066] The obtained mixture was centrifuged, the centrifugation program was 6000rpm, the centrifugation time was 20min, and the centrifugation temperature was 4°C to obtain a supernatant;
[0067] Ste...
Embodiment 2
[0075] This implementation provides the results of antigen dissociation in different batches of CpG ODN composite adjuvant-adsorbed novel coronavirus inactivated vaccines, and examines the applicability of the dissociation method to different batches of samples.
[0076] The specific desorption and antigen potency determination methods are the same as those in Example 1.
[0077] Table 4 shows the test results of antigen potency in different batches of CpG ODN composite adjuvant-adsorbed novel coronavirus inactivated vaccines.
[0078] Table 4
[0079]
[0080] Sample 1-5 double parallel lines result as figure 2 shown.
[0081] As can be seen from Table 4, the present invention has good applicability to the CpG ODN composite adjuvant adsorption type novel coronavirus inactivated vaccine.
Embodiment 3
[0083] This example verifies the applicability of desorption of CpG ODN composite adjuvant-adsorbed new inactivated vaccines using desorption compositions with different component ratios, different desorption conditions, and different centrifugation parameters.
[0084] The theoretical antigen concentration of the sample to be detected used in this example is 13U / ml, and the sample dilution scheme is 2X, 4X, 8X, and 16X. Antigen potency test results between 0.5-2.0 are considered qualified.
[0085] Table 5 shows the desorption conditions used in each sample group and the detection results of antigen potency.
[0086] The specific antigen potency determination operation procedure is the same as that in Example 1.
[0087] table 5
[0088]
[0089] Samples 1-6 double parallel line results such as image 3 shown.
[0090] It can be seen from Table 5 that the test results are all qualified, and it is verified that the desorption composition, the desorption reagent and the ...
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