A kind of creatine kinase isoenzyme detection reagent
A technology for detecting reagents and creatine kinase, which is applied in the field of biological reagents, can solve the problems affecting the accuracy of detection results, poor stability of reagents, affecting the application of reagents, etc., and achieves high clinical application value, high reaction activity, and guaranteed accuracy. Effect
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Embodiment 1
[0068] A creatine kinase isoenzyme detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;
[0069] Reagent R1 is composed of the following ingredients:
[0070] Imidazole buffer (pH=6.0) 50mmo1
[0071] Glucose 45mmo1
[0072] Nanoparticles 40mmol
[0073] N-acetylcysteine 30mmo1
[0074] Sodium edetate 8mmo1
[0075] Adenosine diphosphate 10mmo1
[0076] coenzyme Ⅰ 2mmo1
[0077] Ribonucleotide 10mmo1
[0078] Pyruvate decarboxylase 0.5KU
[0079] Glucose 6-phosphate dehydrogenase 5KU
[0080] Hexokinase 2KU
[0081] Goat anti-human CK-M polyclonal antibody 50mL
[0082] Trehalose 10g
[0083] Add deionized water to 1L;
[0084] Reagent R2 is composed of the following ingredients:
[0085] Imidazole buffer (pH=6.0) 100mmo1
[0086] Phosphocreatine 120mmo1
[0087] Sodium dodecylbenzenesulfonate 20g
[0088] Sodium azide 10g
[0089] Make up to 1 L with deionized water.
[0090] Nanoparticles for γ-Fe 2 o 3 Nanopart...
Embodiment 2
[0096] A creatine kinase isoenzyme detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;
[0097] Reagent R1 is composed of the following ingredients:
[0098] Imidazole buffer (pH=6.5) 80mmo1
[0099] Glucose 32mmo1
[0100] Nanoparticles 30mmol
[0101] N-acetylcysteine 25mmo1
[0102] Sodium edetate 5mmo1
[0103] adenosine diphosphate 5mmo1
[0104] Coenzyme I 4mmo1
[0105] ribonucleotide 15mmo1
[0106] Pyruvate decarboxylase 1.2KU
[0107] Glucose 6-phosphate dehydrogenase 3KU
[0108] Hexokinase 4KU
[0109] Goat anti-human CK-M polyclonal antibody 30mL
[0110] Trehalose 5g
[0111] Add deionized water to 1L;
[0112] Reagent R2 is composed of the following ingredients:
[0113] Imidazole buffer (pH=7.0) 70mmo1
[0114] Phosphocreatine 170mmo1
[0115] Sodium dodecylbenzenesulfonate 10g
[0116] Proclin3006g
[0117] Make up to 1 L with deionized water.
[0118] Nanoparticles for γ-Fe 2 o 3 Nanoparticles...
Embodiment 3
[0121] A creatine kinase isoenzyme detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;
[0122] Reagent R1 is composed of the following ingredients:
[0123] Imidazole buffer (pH=7.0) 100mmo1
[0124] Glucose 20mmo1
[0125] Nanoparticles 20mmol
[0126] N-acetylcysteine 20mmo1
[0127] Sodium edetate 1mmo1
[0128] Adenosine diphosphate 1mmo1
[0129] coenzyme Ⅰ 6mmo1
[0130] Ribonucleotide 25mmo1
[0131] Pyruvate decarboxylase 2KU
[0132] Glucose 6-phosphate dehydrogenase 2KU
[0133] Hexokinase 6KU
[0134] Goat anti-human CK-M polyclonal antibody 10mL
[0135] Trehalose 1g
[0136] Add deionized water to 1L;
[0137] Reagent R2 is composed of the following ingredients:
[0138] Imidazole buffer (pH=6.5) 50mmo1
[0139] Phosphocreatine 220mmo1
[0140] Sodium dodecylbenzenesulfonate 5g
[0141] Proclin3001g
[0142] Make up to 1 L with deionized water.
[0143] Nanoparticles for γ-Fe 2 o 3 Nanoparticles, ...
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