24 results about "Adenosine diphosphate" patented technology

The purpose of the present invention is to provide: a plant having an increased yield of storage tissue; and a method for producing the plant. It was discovered that the yield of storage tissue in a plant can be increased by expressing, in the storage tissue of the plant, a sucrose synthase having a substrate specificity to adenosine diphosphate.

The application belongs to the technical field of molecular biology, and particularly relates to metabolic markers for distinguishing hypertrophic cardiomyopathy and left ventricular hypertrophy and application. The application analyzes the metabolome of hypertrophic cardiomyopathy (HCM) patients, left ventricular hypertrophy (LVH) patients caused by non-hypertrophic cardiomyopathy and healthy people, finds that carnitine C16:2, carnitine C14:0, carnitine C16:0, carnitine C18:0 and carnitine C18:1 are differentially expressed in HCM patients and LVH patients, pyridoxine phosphate, ito acid, adenosine monophosphate, adenosine diphosphate and adenosine triphosphate are differentially expressed in HCM patients and healthy people, lysine base, methyl glutamic acid, acetyl proline, malate semialdehyde and citramalate are differentially expressed in LVH patients and healthy people, and ROC curve analysis shows excellent prediction effect, strong specificity and high sensitivity.

This invention discloses an enzymatic method for preparing (S)-adenosylmethionine. The method uses adenosine diphosphate (ADP) and L-methionine as substrates, reacting them under the combined action of polyphosphate kinase (Ppk) and adenosylmethionine synthase (MAT) to obtain (S)-adenosylmethionine. This method produces a product with high chiral purity and high atom economy, making it more suitable for industrial production.

The application discloses a salt-tolerant adenylate kinase mutant and application thereof. Through modification on an amino acid sequence of a wild-type adenylate kinase (adenylate kinase, EC 2.7.4.3) from Bacillus subtilis (strain 168), three salt-tolerant adenylate kinase mutants are obtained; the mutant adenylate kinase has higher salt tolerance compared with the wild-type adenylate kinase, and can tolerate a salt concentration of 300-2000 mmol / L in an adenosine diphosphate reaction system.

This invention provides a kit for the simultaneous detection of nine flavor nucleotides in livestock and poultry products. The flavor nucleotides are cytidine monophosphate, uridine monophosphate, guanylic acid, inosine monophosphate, adenosine monophosphate, hypoxanthine, inosine, adenosine diphosphate, and adenosine triphosphate. The kit includes: a high-concentration extraction reagent, a standard curve solution, a high-concentration phosphate buffer solution, pH adjustment solution A, and pH adjustment solution B. The high-concentration extraction reagent is perchloric acid; the standard curve solution is a mixed solution of the nine flavor nucleotides; the high-concentration phosphate buffer solution is a solution of potassium dihydrogen phosphate and dipotassium hydrogen phosphate; pH adjustment solution A is sodium hydroxide; and pH adjustment solution B is phosphate. This invention also provides a method for the simultaneous detection of the nine flavor nucleotides using the above kit. This invention enables the simultaneous determination of the content of nine flavor nucleotides in livestock and poultry products. The method is simple, rapid, accurate, and sensitive, and can be used for batch determination, showing good prospects for practical application.

The invention relates to the technical field of biological detection, in particular to a determination method of ABHD6 enzyme activity. The method comprises the following steps: a) catalyzing 2-arachidonyl glycerol (2-AG) to hydrolyze by ABHD6 to generate glycerol; b) adding glycerol kinase and adenosine triphosphate (ATP), and enabling the glycerol to generate glycerol-1-phosphoric acid (G-1-P) and adenosine diphosphate (ADP) under the catalysis of the glycerol kinase; and c) carrying out quantitative detection on ADP by adopting an ADP-Glo detection kit, and deducing the ABHD6 enzyme activity through the chemiluminescence signal intensity. Compared with an existing ABHD6 activity detection method based on radioactive labeling, mass spectrometry or fluorescence coupling, the ABHD6 activity detection method has the advantages that the ABHD6-glycerol kinase-ADP-Glo luminous system is established, so that the detection sensitivity, stability and flux are remarkably improved.

The application discloses four kinds of tetra-cyclic compounds with selective adenosine diphosphate inhibition, which have the following formula structure, wherein the amino acids represented by AA are L-Ser residues, L-Glu residues, L-Lys residues and L-Tyr residues respectively, the application discloses a preparation method of the tetra-cyclic compounds and application of the tetra-cyclic compounds in treating arterial thrombosis diseases. Experiments prove that the adenosine diphosphate selective inhibitor has good anti-arterial thrombosis effect. Thus, the application provides an effective technical means for resisting arterial thrombosis.

The present application relates to the technical field of enzyme, in particular to a mutant of BsBacD enzyme, an enzyme composition and a preparation method of tyrosine dipeptide. The present application firstly rationally designs the BsBacD enzyme, then replaces ADP (adenosine diphosphate or its salt) with relatively cheap ATP (adenosine triphosphate or its salt) or even cheaper AMP (adenosine monophosphate or its salt), and introduces polyphosphate kinase ChPPK2 which can regenerate ATP with AMP as a substrate to synthesize tyrosine dipeptide, and optimizes reaction pH, magnesium chloride concentration and substrate tyrosine concentration and other parameters, and develops a low-cost and high-efficiency enzyme synthesis process of three kinds of tyrosine dipeptides, such as propyl tyrosine dipeptide, glycosyl tyrosine dipeptide and silk tyrosine dipeptide.

The application discloses a method for preparing 2'-methoxy-uridine triphosphate, which comprises the following steps: taking 2'-methoxy-uridine acid as a substrate, taking adenosine triphosphate as an initial phosphate donor, constructing a phosphate circulation system, adding an enzyme catalyst, and adopting one-pot enzyme method to perform enzyme catalysis reaction, so as to obtain the end product 2'-methoxy-uridine triphosphate. The phosphate circulation system is composed of adenosine triphosphate, adenosine diphosphate and phosphate compounds. The enzyme catalyst catalyzes the dephosphorylation of the phosphate compounds to provide inorganic phosphate to the adenosine diphosphate, and the adenosine triphosphate is regenerated to realize the circulation energy supply. The method adopts high-activity enzyme combination of specific sources, has high conversion rate of the substrate 2'-methoxy-uridine acid, and has high conversion rate of the final 2'-methoxy-uridine triphosphate up to 92.6%, and has the advantages of high synthesis efficiency, simple steps, green environmental protection and controllable cost.

The invention discloses a method for visually detecting various phosphates, which comprises the following steps: firstly, synthesizing three MOF (Metal Organic Framework) nano-enzymes, namely PCN-222 (Fe), PCN-223 (Fe) and PCN-224 (Fe) with peroxidase-like activity, catalyzing a colorless substrate 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) into oxTMB in a blue oxidation state by the three MOF nano-enzymes in the presence of H2O2, and generating remarkable colorimetric response; then, the five phosphates, namely ATP (adenosine triphosphate), ADP (adenosine diphosphate), AMP (adenosine monophosphate), PPi (pyrophosphoric acid) and Pi (inorganic phosphate), are simultaneously distinguished and detected by utilizing the principle that different phosphates have different regulation degrees on the peroxidase-like activity of the MOF nano-enzyme. In combination with a smartphone application program 'ColorMax', real-time visual analysis of phosphate is realized, and an efficient and convenient method is provided for distinguishing and detecting different phosphates.

The invention discloses phycocyanin peptide as well as a screening method and application thereof, and belongs to the technical field of bioactive peptides. The peptide sequences of the phycocyanin peptide are ASEIAG, LDSVNR, AEQPQL and AGDASV (Adenosine Diphosphate Syndrome Virus); the screening method specifically comprises the following steps: (1) virtual enzymolysis of phycocyanin; (2) screening the bioactive peptide; and (3) molecular docking. According to the invention, four bifunctional peptides ASEIAG, LDSVNR, AEQPQL and AGDASV, which are brand new in structure, clear in activity and easy to absorb, are accurately excavated from a specific amino acid sequence of phycocyanin through a rational design method in combination with gastrointestinal tract digestion simulation (virtual enzymolysis) and multi-target computer-aided screening (molecular docking); the method can simultaneously aim at two mutually associated pathological links of hypertension and oxidative stress, has the unique advantage of synergistic interaction in the aspect of preventing cardiovascular and cerebrovascular diseases, and breaks through the limitation of a traditional enzymolysis technology; and a brand new core material basis is provided for developing the next generation of functional food or ingredients for reducing blood pressure and resisting oxidation based on precise nutrition.

This invention relates to the field of biological agents, and more particularly to the application of adenosine diphosphate; it provides the application of adenosine diphosphate in the preparation of drugs for treating bone defects; it provides a preparation for treating bone defects, comprising: adenosine diphosphate; the preparation of this invention does not require the collection of the patient's own bone marrow and peripheral blood, and will not cause additional damage or pain to the patient; the preparation containing adenosine diphosphate can accelerate bone tissue healing more conveniently and safely, providing conditions for early weight-bearing and functional exercises after surgery, and improving the patient's quality of life; the preparation method of the preparation of this invention is simple and easy to operate.

Metabolic biomarkers and their application in the preparation of diagnostic products for carotid body tumors demonstrate high specificity and sensitivity in diagnostic efficacy. These metabolic biomarkers include at least one of the following substances: S-adenosylmethionine, S-sulfocysteine, cystine, adenosine diphosphate, and lactate.

To provide a plant with increased yield of storage tissue and a method for producing the same. [Solution] In the storage tissue of a plant, a sucrose synthase that has substrate specificity for adenosine diphosphate is expressed, thereby increasing the yield of the storage tissue of the plant.

The invention discloses a dragon bone active peptide for promoting male reproductive function development as well as a preparation method and application thereof, and belongs to the technical field of active peptides. The active peptide of the dragon's tendon comprises at least one of ADLF (adenosine diphosphate linolenic acid), FGAGGFA (fibroblast growth factor growth factor A), GPGPTP (general purpose GPGPTP), GPDGY (fibroblast growth factor Y), LEGF (growth factor growth factor), QPGSL, AFGAA (adenosine triphosphate), GEPGL (glutathione), AGPPVGQP, QLGL or SPSL. According to the invention, sturgeon keel is taken as a raw material, polypeptide with the highest activity of promoting male reproductive function development is selected from different enzymolysis combinations through cell tests, and potential sturgeon keel active peptide with the function of promoting male reproductive function development is screened out through bioinformatics technologies such as mass spectrum identification of the sequence, molecular docking and the like. The dragon bone active peptide can solve the problem of high-value utilization of sturgeons, can be applied to functional products as a functional factor, and has a good application prospect.

The invention discloses a method for preparing 2 '-methoxy-uridine triphosphate, which comprises the following steps: in a liquid reaction system, constructing a phosphoric acid circulating system by taking 2'-methoxy-uridine monophosphate as a substrate and adenosine triphosphate as an initial phosphoric acid donor, adding an enzyme catalyst, and carrying out an enzyme catalysis reaction by adopting a one-pot enzyme method to obtain the 2 '-methoxy-uridine triphosphate. The phosphoric acid circulating system is composed of adenosine triphosphate, adenosine diphosphate and a phosphate compound, inorganic phosphoric acid is provided for adenosine diphosphate by catalyzing dephosphorizing of the phosphate compound through the enzyme catalyst, and adenosine triphosphate is regenerated to achieve circulating energy supply. According to the method, a high-activity enzyme combination with a specific source is adopted, the conversion rate of the substrate 2 '-methoxyl-uridine monophosphate is high, the final conversion rate of 2'-methoxyl-uridine triphosphate reaches up to 92.6%, and the method has the advantages of being high in synthesis efficiency, simple in step, environmentally friendly and controllable in cost.

The present application relates to the field of biological catalysis, and discloses a method for preparing creatine phosphate by immobilized enzyme catalysis, which comprises the following steps: mixing gelatin and a positive charge modifier to obtain a carrier liquid; adding creatine and magnesium salt to a crude creatine kinase solution for pre-incubation to realize conformational locking, then mixing with the carrier liquid and dropping into a low-temperature coagulation bath to solidify into gel microspheres, and then cross-linking and washing to obtain immobilized creatine kinase particles; finally, adding substrates, sodium hexametaphosphate, polyphosphate kinase and immobilized particles into a reaction system for reaction, and then separating and collecting the supernatant to obtain creatine phosphate. The present application protects the three-dimensional conformation of the active center of the enzyme by pre-incubation, uses the positive micro area of the carrier to electrostatically enrich the substrates to accelerate mass transfer, and introduces an auxiliary enzyme to construct an in-situ substrate regeneration system to re-phosphorylate the by-product adenosine diphosphate into adenosine triphosphate. The present application eliminates the feedback inhibition of the product, reduces the cost of raw materials, and improves the overall catalytic efficiency.

Antibodies that interact with FcγRIIa are described. Methods of detecting the amount of FcγRIIa on platelets in a sample using antibodies to FcγRIIa are described. Methods of treating a selected subject at risk of thrombosis with an anti-thrombotic therapy are described involving administering aspirin, cilostazol or an anti-thrombotic agent that is an Adenosine diphosphate (ADP) receptor antagonist and / or a Protease-activated receptor (PAR) antagonist to the selected subject, wherein the subject is selected by determining a level of FcγRIIa expressed on platelets by detecting the amount of FcγRIIa in a sample using antibodies to FcγRIIa. In certain methods, elevated levels of FcγRIIa expression identify the subject as at risk of thrombosis and in need for anti-thrombotic therapy.

The invention provides a kit for detecting pyruvate kinase and application, and belongs to the technical field of pyruvate kinase detection. The kit comprises a cell ATP removal reagent and a pyruvate kinase detection reagent, the cell ATP removal reagent comprises adenylate cyclase; the pyruvate kinase detection reagent comprises a bioluminescent enzyme, a bioluminescent enzyme substrate, phosphoenolpyruvic acid, adenosine diphosphate and a non-PK kinase inhibitor; the non-PK kinase inhibitor is a myokinase inhibitor and / or a nucleoside diphosphate kinase inhibitor. The kit for detecting the pyruvate kinase can be used for qualitatively or quantitatively detecting the PK activity in a sample. When the kit is used for detecting the light-emitting signal generated by the ATP of the PK reaction product, the kit is suitable for long-time detection, and the enzyme activity is in positive correlation with the amount of the ATP. The kit provided by the invention can be used for detecting the enzyme activity of PK in different samples, and has the advantages of high sensitivity, high specificity, good linear relationship and stable fluorescence signal.

The invention provides a UDP-glucosyltransferase mutant and a method for preparing rebaudioside D. The UDP-glucosyltransferase mutant is subjected to site-directed mutagenesis, mutant plasmid is transferred to escherichia coli to obtain recombinant engineering bacteria, the recombinant engineering bacteria are subjected to protein expression through fermentation, and UDP-glucosyltransferase mutant crude enzyme is obtained. The ADP-dependent enzyme activity and temperature of the UDP-glucosyltransferase mutant are measured, the ADP-dependent enzyme activity after mutation is remarkably improved, and the mutant has high temperature resistance. The rebaudioside D is prepared from rebaudioside A, sucrose, ADP (adenosine diphosphate), a UDP-glucosyltransferase mutant and AtSUS sucrose synthetase high-catalysis synthetase.

The present application relates to the use of cyclic adenosine diphosphate (ADP) ribose hydrolase (CD38) inhibitors and enteroendocrine-pancreatic hormone pathway modulators for preventing or treating diseases and disorders of metabolism.

This invention relates to an in vitro maturation culture medium for giant panda oocytes and its application, belonging to the field of biotechnology. The invention discloses an in vitro maturation culture medium for giant panda oocytes, which simultaneously adds the following three paracrine factors to the basal culture medium: adenosine diphosphate riboylation factor 6 (ARF6); ciliary neurotrophic factor (CNTF); and hepatocyte growth factor (HGF). The invention also provides the application of the aforementioned in vitro maturation culture medium for giant panda oocytes, specifically for the in vitro maturation culture of giant panda oocytes. This invention effectively addresses the shortcomings of existing in vitro oocyte maturation technologies, significantly improves oocyte maturation quality, is simple to operate, and has high safety, providing a key technological reserve for assisted reproduction in giant pandas.

This invention belongs to the field of cosmetic technology, specifically relating to an anti-wrinkle and firming composition, its preparation method, and its application. The composition comprises the following raw materials in the indicated weight percentages: 0.1-0.2% acetyl hexapeptide-8, 0.3-0.5% *Gynostemma pentaphyllum* polysaccharide extract, 0.051-0.069% ethylhexylglycerin, 0.53-0.55% phenoxyethanol, 25-35% glycerin, and the balance being water. In this invention, *Gynostemma pentaphyllum* algae are inoculated into BG-11 medium supplemented with inositol and cultured to obtain *Gynostemma pentaphyllum* spheroids. These spheroids are then inoculated into BG-11 medium supplemented with calcium adenosine diphosphate and cultured to obtain *Gynostemma pentaphyllum* powder. Finally, *Gynostemma pentaphyllum* polysaccharide is extracted using a water extraction and alcohol precipitation method. By improving the *Gynostemma pentaphyllum* cultivation process, high-quality *Gynostemma pentaphyllum* powder is obtained, thereby increasing the yield of *Gynostemma pentaphyllum* polysaccharide. The composition containing *Gynostemma pentaphyllum* polysaccharide extract is safe to use and exhibits excellent anti-wrinkle and firming effects.

The invention relates to a preparation method of a poly (adenosine diphosphate-ribose) polymerase inhibitor intermediate, and the method comprises the following steps: firstly, removing an acetyl protecting group by CH3OLi to prepare a compound H-1, and then hydrolyzing methyl ester by LiOH to obtain a compound Y. Compared with a traditional process method, the method has the advantages that the yield is high, and the yield is high. The step-by-step hydrolysis method can greatly reduce and eliminate byproducts generated in the reaction, and the content of the byproducts is from gt; the reaction temperature is reduced to 1% or below from 10%, and the total reaction yield is improved.