40 results about "Adenosine diphosphate" patented technology

To provide a novel platelet aggregation inhibitor.SOLUTION: The present invention has the following features to solve the above problem. 1. A collagen-induced platelet aggregation inhibitor, comprising an extract of yellow strawberry guava (Psidium littorale) fruit as an active ingredient. 2. An adenosine diphosphate-induced platelet aggregation inhibitor, comprising an extract of Psidium littorale fruit as an active ingredient. 3. An induced platelet aggregation inhibitor, comprising an extract of Psidium littorale fruit as an active ingredient. 4. The agent described in any one of (1) to (3), wherein the extract of Psidium littorale fruit comprises adenosine. 5. An extract of Psidium littorale fruit, comprising adenosine.SELECTED DRAWING: None

The invention discloses a CK-MB-containing biochemical multi-term liquid quality control product and a preparation method thereof, and belongs to the technical field of biochemical quality control products, the added substances comprise an adenosine phosphate compound, a creatine compound and an auxiliary stabilizer, the adenosine phosphate compound is at least one of adenosine diphosphate and adenosine triphosphate, and the creatine compound is at least one of creatine triphosphate and creatine triphosphate. The creatine compound is at least one of creatine and phosphocreatine, and the auxiliary stabilizer is at least one of acetylcysteine and beta-mercaptoethanol; and the CK-MB-containing biochemical multi-liquid quality control product is a frozen liquid. The adenylate phosphate compound, the creatine compound and the auxiliary stabilizer are added into the liquid quality control product, so that multiple protection mechanisms can be realized, and the stability of multiple biochemical liquid quality control products containing CK-MB, especially the stability of CK-MB, is obviously improved; errors caused by redissolution are avoided, the detection convenience and accuracy of inspection personnel are greatly improved, and the labor and time cost of a laboratory is reduced.

The purpose of the present invention is to provide: a plant having an increased yield of storage tissue; and a method for producing the plant. It was discovered that the yield of storage tissue in a plant can be increased by expressing, in the storage tissue of the plant, a sucrose synthase having a substrate specificity to adenosine diphosphate.

The application belongs to the technical field of molecular biology, and particularly relates to metabolic markers for distinguishing hypertrophic cardiomyopathy and left ventricular hypertrophy and application. The application analyzes the metabolome of hypertrophic cardiomyopathy (HCM) patients, left ventricular hypertrophy (LVH) patients caused by non-hypertrophic cardiomyopathy and healthy people, finds that carnitine C16:2, carnitine C14:0, carnitine C16:0, carnitine C18:0 and carnitine C18:1 are differentially expressed in HCM patients and LVH patients, pyridoxine phosphate, ito acid, adenosine monophosphate, adenosine diphosphate and adenosine triphosphate are differentially expressed in HCM patients and healthy people, lysine base, methyl glutamic acid, acetyl proline, malate semialdehyde and citramalate are differentially expressed in LVH patients and healthy people, and ROC curve analysis shows excellent prediction effect, strong specificity and high sensitivity.

The invention relates to the technical field of enzymes, in particular to a BsBacD enzyme mutant, an enzyme composition and a preparation method of tyrosine dipeptide. The method comprises the following steps: rationally designing BsBacD enzyme, replacing ADP (adenosine diphosphate or salt thereof) with relatively cheap ATP (adenosine triphosphate or salt thereof) or relatively cheap AMP (adenosine monophosphate or salt thereof), and introducing polyphosphate kinase ChPPK2 capable of regenerating ATP by taking AMP as a substrate to synthesize tyrosine dipeptide; parameters such as reaction pH, magnesium chloride concentration, substrate tyrosine concentration and the like are optimized, and a low-cost and efficient enzymatic synthesis process of three tyrosine dipeptides such as propyl tyrosine dipeptide, glycyl tyrosine dipeptide and sericin tyrosine dipeptide is developed.

The invention provides a method for simultaneously determining various flavor nucleotides in a livestock and poultry meat product and application, and the method comprises the following steps: freeze-drying the livestock and poultry meat product, and then preparing the freeze-dried livestock and poultry meat product into a test solution; measuring the contents of CMP (cytidylic acid), ATP (adenosine triphosphate), GMP (guanine monophosphate), IMP (inosinic acid), ADP (adenosine diphosphate), Hy (hypoxanthine), AMP (adenosine monophosphate) and IN (inosine) in the test solution by adopting HPLC (high performance liquid chromatography); in HPLC, a mobile phase A is a phosphate buffer solution with the volume concentration of 95%, a mobile phase B is methanol with the volume concentration of 5%, gradient elution is carried out, and the gradient elution condition is 0-11 min, 99% phosphate buffer solution and 1% methanol; when 12 min, 80% phosphate buffer solution and 20% methanol are added, and the conditions are maintained for 6-7 min; the phosphate buffer solution is heated to 99% within 2 minutes, and then the temperature is kept for 8-10 minutes.

This invention discloses an enzymatic method for preparing (S)-adenosylmethionine. The method uses adenosine diphosphate (ADP) and L-methionine as substrates, reacting them under the combined action of polyphosphate kinase (Ppk) and adenosylmethionine synthase (MAT) to obtain (S)-adenosylmethionine. This method produces a product with high chiral purity and high atom economy, making it more suitable for industrial production.

The application discloses a salt-tolerant adenylate kinase mutant and application thereof. Through modification on an amino acid sequence of a wild-type adenylate kinase (adenylate kinase, EC 2.7.4.3) from Bacillus subtilis (strain 168), three salt-tolerant adenylate kinase mutants are obtained; the mutant adenylate kinase has higher salt tolerance compared with the wild-type adenylate kinase, and can tolerate a salt concentration of 300-2000 mmol / L in an adenosine diphosphate reaction system.

To provide an ADP (adenosine diphosphate)-ribose-binding peptide having anticancer activity.SOLUTION: Provided are an ADP-ribose-binding peptide of a specific amino acid sequence and a denatured object thereof, and a pharmaceutical composition for preventing or treating cancer and a pharmaceutical composition for assisting anti-cancer activity which contain them as an active ingredient. The ADP-ribose-binding peptide breaks equilibrium of a cell to kill a cancer cell by accumulating ADP-ribose in a cancer cell, and has excellent anti-cancer effect that does not express toxicity in a normal cell, and in the case where the peptide is administered in combination with administration of other anticancer agent or anti-cancer radiotherapy, it enhances responsiveness to the anticancer agent and radiotherapy, and has extremely excellent effect as an anti-cancer adjuvant.SELECTED DRAWING: None

The invention discloses an application of 3-aminopropyltriethoxysilane modified graphene oxide in reduction of ADN (adenosine diphosphate) sensitivity and an application of the 3-aminopropyltriethoxysilane modified graphene oxide in reduction of ADN sensitivity. According to the invention, the 3-aminopropyltriethoxysilane modified graphene oxide is used, so that the impact sensitivity of ammonium dinitramide is reduced, the decomposition of ADN is promoted, and the thermal decomposition performance is improved. The further prepared compound not only can reduce the impact sensitivity and friction sensitivity of the ADN, but also can promote decomposition of the ADN and improve the mass loss of the ADN.

The invention relates to a divisional application with the application number of 202411710591.1, discloses a marker for diagnosis or prognosis evaluation of children with epilepsy as well as a detection method and application of the marker, and belongs to the field of detection. The marker comprises one or more of the following purine metabolites: uric acid, creatinine, xanthine, hypoxanthine, guanine, adenine, adenosine, adenosine triphosphate, adenosine diphosphate and adenylic acid. The difference of blood / urine purine metabolite concentrations of an epilepsy child and a healthy control is reported, a disease prediction model based on the difference of various indexes of blood / urine purine is constructed, a comprehensive scoring algorithm is established, a detection result is convenient to interpret, and a clinical basis is provided for diagnosis and prognosis evaluation of child epileptic seizure.

This invention provides a kit for the simultaneous detection of nine flavor nucleotides in livestock and poultry products. The flavor nucleotides are cytidine monophosphate, uridine monophosphate, guanylic acid, inosine monophosphate, adenosine monophosphate, hypoxanthine, inosine, adenosine diphosphate, and adenosine triphosphate. The kit includes: a high-concentration extraction reagent, a standard curve solution, a high-concentration phosphate buffer solution, pH adjustment solution A, and pH adjustment solution B. The high-concentration extraction reagent is perchloric acid; the standard curve solution is a mixed solution of the nine flavor nucleotides; the high-concentration phosphate buffer solution is a solution of potassium dihydrogen phosphate and dipotassium hydrogen phosphate; pH adjustment solution A is sodium hydroxide; and pH adjustment solution B is phosphate. This invention also provides a method for the simultaneous detection of the nine flavor nucleotides using the above kit. This invention enables the simultaneous determination of the content of nine flavor nucleotides in livestock and poultry products. The method is simple, rapid, accurate, and sensitive, and can be used for batch determination, showing good prospects for practical application.

The invention relates to the technical field of biological detection, in particular to a determination method of ABHD6 enzyme activity. The method comprises the following steps: a) catalyzing 2-arachidonyl glycerol (2-AG) to hydrolyze by ABHD6 to generate glycerol; b) adding glycerol kinase and adenosine triphosphate (ATP), and enabling the glycerol to generate glycerol-1-phosphoric acid (G-1-P) and adenosine diphosphate (ADP) under the catalysis of the glycerol kinase; and c) carrying out quantitative detection on ADP by adopting an ADP-Glo detection kit, and deducing the ABHD6 enzyme activity through the chemiluminescence signal intensity. Compared with an existing ABHD6 activity detection method based on radioactive labeling, mass spectrometry or fluorescence coupling, the ABHD6 activity detection method has the advantages that the ABHD6-glycerol kinase-ADP-Glo luminous system is established, so that the detection sensitivity, stability and flux are remarkably improved.

The present invention discloses a steel bar rust inhibitor for use in complex chloride environments, as well as its preparation and use methods. The steel bar rust inhibitor comprises a solvent and a solute dissolved therein, wherein the solvent is hydrolase-free double-distilled water with a pH greater than 6.0; the solute comprises, by mass percentage, 80%-90% DAP dry powder and 10%-20% PVA powder; wherein the DAP is prepared by grafting adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate onto a nucleoside phosphorous acid activated intermediate. Compared with existing rust inhibitors, the present invention utilizes a more simplified synthesis method, which, while ensuring significant rust inhibition, avoids the limitations of the use environment and the agglomeration phenomenon caused by excessive molecular weight.

The application discloses four kinds of tetra-cyclic compounds with selective adenosine diphosphate inhibition, which have the following formula structure, wherein the amino acids represented by AA are L-Ser residues, L-Glu residues, L-Lys residues and L-Tyr residues respectively, the application discloses a preparation method of the tetra-cyclic compounds and application of the tetra-cyclic compounds in treating arterial thrombosis diseases. Experiments prove that the adenosine diphosphate selective inhibitor has good anti-arterial thrombosis effect. Thus, the application provides an effective technical means for resisting arterial thrombosis.

The present application relates to the technical field of enzyme, in particular to a mutant of BsBacD enzyme, an enzyme composition and a preparation method of tyrosine dipeptide. The present application firstly rationally designs the BsBacD enzyme, then replaces ADP (adenosine diphosphate or its salt) with relatively cheap ATP (adenosine triphosphate or its salt) or even cheaper AMP (adenosine monophosphate or its salt), and introduces polyphosphate kinase ChPPK2 which can regenerate ATP with AMP as a substrate to synthesize tyrosine dipeptide, and optimizes reaction pH, magnesium chloride concentration and substrate tyrosine concentration and other parameters, and develops a low-cost and high-efficiency enzyme synthesis process of three kinds of tyrosine dipeptides, such as propyl tyrosine dipeptide, glycosyl tyrosine dipeptide and silk tyrosine dipeptide.

The application discloses a method for preparing 2'-methoxy-uridine triphosphate, which comprises the following steps: taking 2'-methoxy-uridine acid as a substrate, taking adenosine triphosphate as an initial phosphate donor, constructing a phosphate circulation system, adding an enzyme catalyst, and adopting one-pot enzyme method to perform enzyme catalysis reaction, so as to obtain the end product 2'-methoxy-uridine triphosphate. The phosphate circulation system is composed of adenosine triphosphate, adenosine diphosphate and phosphate compounds. The enzyme catalyst catalyzes the dephosphorylation of the phosphate compounds to provide inorganic phosphate to the adenosine diphosphate, and the adenosine triphosphate is regenerated to realize the circulation energy supply. The method adopts high-activity enzyme combination of specific sources, has high conversion rate of the substrate 2'-methoxy-uridine acid, and has high conversion rate of the final 2'-methoxy-uridine triphosphate up to 92.6%, and has the advantages of high synthesis efficiency, simple steps, green environmental protection and controllable cost.

The invention discloses a method for visually detecting various phosphates, which comprises the following steps: firstly, synthesizing three MOF (Metal Organic Framework) nano-enzymes, namely PCN-222 (Fe), PCN-223 (Fe) and PCN-224 (Fe) with peroxidase-like activity, catalyzing a colorless substrate 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) into oxTMB in a blue oxidation state by the three MOF nano-enzymes in the presence of H2O2, and generating remarkable colorimetric response; then, the five phosphates, namely ATP (adenosine triphosphate), ADP (adenosine diphosphate), AMP (adenosine monophosphate), PPi (pyrophosphoric acid) and Pi (inorganic phosphate), are simultaneously distinguished and detected by utilizing the principle that different phosphates have different regulation degrees on the peroxidase-like activity of the MOF nano-enzyme. In combination with a smartphone application program 'ColorMax', real-time visual analysis of phosphate is realized, and an efficient and convenient method is provided for distinguishing and detecting different phosphates.

The invention discloses phycocyanin peptide as well as a screening method and application thereof, and belongs to the technical field of bioactive peptides. The peptide sequences of the phycocyanin peptide are ASEIAG, LDSVNR, AEQPQL and AGDASV (Adenosine Diphosphate Syndrome Virus); the screening method specifically comprises the following steps: (1) virtual enzymolysis of phycocyanin; (2) screening the bioactive peptide; and (3) molecular docking. According to the invention, four bifunctional peptides ASEIAG, LDSVNR, AEQPQL and AGDASV, which are brand new in structure, clear in activity and easy to absorb, are accurately excavated from a specific amino acid sequence of phycocyanin through a rational design method in combination with gastrointestinal tract digestion simulation (virtual enzymolysis) and multi-target computer-aided screening (molecular docking); the method can simultaneously aim at two mutually associated pathological links of hypertension and oxidative stress, has the unique advantage of synergistic interaction in the aspect of preventing cardiovascular and cerebrovascular diseases, and breaks through the limitation of a traditional enzymolysis technology; and a brand new core material basis is provided for developing the next generation of functional food or ingredients for reducing blood pressure and resisting oxidation based on precise nutrition.

This invention relates to the field of biological agents, and more particularly to the application of adenosine diphosphate; it provides the application of adenosine diphosphate in the preparation of drugs for treating bone defects; it provides a preparation for treating bone defects, comprising: adenosine diphosphate; the preparation of this invention does not require the collection of the patient's own bone marrow and peripheral blood, and will not cause additional damage or pain to the patient; the preparation containing adenosine diphosphate can accelerate bone tissue healing more conveniently and safely, providing conditions for early weight-bearing and functional exercises after surgery, and improving the patient's quality of life; the preparation method of the preparation of this invention is simple and easy to operate.

Metabolic biomarkers and their application in the preparation of diagnostic products for carotid body tumors demonstrate high specificity and sensitivity in diagnostic efficacy. These metabolic biomarkers include at least one of the following substances: S-adenosylmethionine, S-sulfocysteine, cystine, adenosine diphosphate, and lactate.

The invention discloses an application of Linsitinib as an ADV7 (adenosine diphosphate 7) virus inhibitor. According to an anti-ADV7 activity research experiment of the Linsitinib, the compound Linsitinib inhibits the cytopathic effect (CPE) generated by ADV7 on a host cell Hela, the cell survival rate is enhanced, the progeny virus yield is reduced, and the compound Linsitinib has the potential to be applied to preparation of anti-ADV7 virus drugs.

The invention discloses application of polyphosphate kinase BsPPK in synthesis of ATP (adenosine triphosphate). The amino acid sequence of the polyphosphate kinase BsPPK is as shown in SEQ ID NO. 1. In specific application, adenosine diphosphate or adenosine monophosphate is used as a phosphate receptor, polyphosphate is used as a phosphoric acid donor, and ATP is synthesized under the action of polyphosphate kinase BsPPK. The polyphosphate is selected from tripolyphosphate or hexametaphosphate. The invention also discloses an application of the polyphosphate kinase BsPPK in the synthesis of uridine diphosphate-galactose, and an application of the polyphosphate kinase BsPPK in the synthesis of lactosyl-N-tetrasaccharide. According to the method, the ATP is synthesized and regenerated by utilizing the polyphosphate kinase BsPPK, so that the reaction cost of UDP-Gal and LNT in-vitro synthesis routes is greatly reduced. The method has important significance on ATP regeneration and synthesis of UDP-Gal and galactosyl lactose derivatives, and has a wide application prospect.

To provide a plant with increased yield of storage tissue and a method for producing the same. [Solution] In the storage tissue of a plant, a sucrose synthase that has substrate specificity for adenosine diphosphate is expressed, thereby increasing the yield of the storage tissue of the plant.

The invention discloses a dragon bone active peptide for promoting male reproductive function development as well as a preparation method and application thereof, and belongs to the technical field of active peptides. The active peptide of the dragon's tendon comprises at least one of ADLF (adenosine diphosphate linolenic acid), FGAGGFA (fibroblast growth factor growth factor A), GPGPTP (general purpose GPGPTP), GPDGY (fibroblast growth factor Y), LEGF (growth factor growth factor), QPGSL, AFGAA (adenosine triphosphate), GEPGL (glutathione), AGPPVGQP, QLGL or SPSL. According to the invention, sturgeon keel is taken as a raw material, polypeptide with the highest activity of promoting male reproductive function development is selected from different enzymolysis combinations through cell tests, and potential sturgeon keel active peptide with the function of promoting male reproductive function development is screened out through bioinformatics technologies such as mass spectrum identification of the sequence, molecular docking and the like. The dragon bone active peptide can solve the problem of high-value utilization of sturgeons, can be applied to functional products as a functional factor, and has a good application prospect.

The invention discloses a method for preparing 2 '-methoxy-uridine triphosphate, which comprises the following steps: in a liquid reaction system, constructing a phosphoric acid circulating system by taking 2'-methoxy-uridine monophosphate as a substrate and adenosine triphosphate as an initial phosphoric acid donor, adding an enzyme catalyst, and carrying out an enzyme catalysis reaction by adopting a one-pot enzyme method to obtain the 2 '-methoxy-uridine triphosphate. The phosphoric acid circulating system is composed of adenosine triphosphate, adenosine diphosphate and a phosphate compound, inorganic phosphoric acid is provided for adenosine diphosphate by catalyzing dephosphorizing of the phosphate compound through the enzyme catalyst, and adenosine triphosphate is regenerated to achieve circulating energy supply. According to the method, a high-activity enzyme combination with a specific source is adopted, the conversion rate of the substrate 2 '-methoxyl-uridine monophosphate is high, the final conversion rate of 2'-methoxyl-uridine triphosphate reaches up to 92.6%, and the method has the advantages of being high in synthesis efficiency, simple in step, environmentally friendly and controllable in cost.

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle firming composition as well as a preparation method and application thereof. The composition is prepared from the following raw materials in percentage by mass: 0.1 to 0.2 percent of acetyl hexapeptide-8, 0.3 to 0.5 percent of nostoc sphaeroides polysaccharide extract, 0.051 to 0.069 percent of ethylhexylglycerin, 0.53 to 0.55 percent of phenoxyethanol, 25 to 35 percent of glycerin and the balance of water. The method comprises the following steps: inoculating nostoc sphaeroids kutz into a BG-11 culture medium added with inositol for culturing to obtain nostoc sphaeroids kutz spheroids, inoculating the nostoc sphaeroids kutz spheroids into a BG-11 culture medium added with calcium adenosine diphosphate for culturing to obtain nostoc sphaeroids kutz powder, and finally extracting nostoc sphaeroids kutz polysaccharide by a water extraction and alcohol precipitation method. By improving the cultivation process of the nostoc sphaeroides, the high-quality nostoc sphaeroides powder is obtained, and the yield of the nostoc sphaeroides polysaccharide is further improved. The composition containing the nostoc sphaeroids kutz polysaccharide extract is safe to use and has excellent anti-wrinkle and firming effects.

The present application relates to the field of biological catalysis, and discloses a method for preparing creatine phosphate by immobilized enzyme catalysis, which comprises the following steps: mixing gelatin and a positive charge modifier to obtain a carrier liquid; adding creatine and magnesium salt to a crude creatine kinase solution for pre-incubation to realize conformational locking, then mixing with the carrier liquid and dropping into a low-temperature coagulation bath to solidify into gel microspheres, and then cross-linking and washing to obtain immobilized creatine kinase particles; finally, adding substrates, sodium hexametaphosphate, polyphosphate kinase and immobilized particles into a reaction system for reaction, and then separating and collecting the supernatant to obtain creatine phosphate. The present application protects the three-dimensional conformation of the active center of the enzyme by pre-incubation, uses the positive micro area of the carrier to electrostatically enrich the substrates to accelerate mass transfer, and introduces an auxiliary enzyme to construct an in-situ substrate regeneration system to re-phosphorylate the by-product adenosine diphosphate into adenosine triphosphate. The present application eliminates the feedback inhibition of the product, reduces the cost of raw materials, and improves the overall catalytic efficiency.

Antibodies that interact with FcγRIIa are described. Methods of detecting the amount of FcγRIIa on platelets in a sample using antibodies to FcγRIIa are described. Methods of treating a selected subject at risk of thrombosis with an anti-thrombotic therapy are described involving administering aspirin, cilostazol or an anti-thrombotic agent that is an Adenosine diphosphate (ADP) receptor antagonist and / or a Protease-activated receptor (PAR) antagonist to the selected subject, wherein the subject is selected by determining a level of FcγRIIa expressed on platelets by detecting the amount of FcγRIIa in a sample using antibodies to FcγRIIa. In certain methods, elevated levels of FcγRIIa expression identify the subject as at risk of thrombosis and in need for anti-thrombotic therapy.

The invention provides a kit for detecting pyruvate kinase and application, and belongs to the technical field of pyruvate kinase detection. The kit comprises a cell ATP removal reagent and a pyruvate kinase detection reagent, the cell ATP removal reagent comprises adenylate cyclase; the pyruvate kinase detection reagent comprises a bioluminescent enzyme, a bioluminescent enzyme substrate, phosphoenolpyruvic acid, adenosine diphosphate and a non-PK kinase inhibitor; the non-PK kinase inhibitor is a myokinase inhibitor and / or a nucleoside diphosphate kinase inhibitor. The kit for detecting the pyruvate kinase can be used for qualitatively or quantitatively detecting the PK activity in a sample. When the kit is used for detecting the light-emitting signal generated by the ATP of the PK reaction product, the kit is suitable for long-time detection, and the enzyme activity is in positive correlation with the amount of the ATP. The kit provided by the invention can be used for detecting the enzyme activity of PK in different samples, and has the advantages of high sensitivity, high specificity, good linear relationship and stable fluorescence signal.