64 results about "L-Carnosine" patented technology

A dietary supplement of mitochondrial nutrients is designed for relieving stress, preventing and improving stress-related disorders, such as chronic fatigue syndrome, diabetes, age-associated cognitive dysfunction and diseases (Parkinson's and Alzheimer's disease). The supplement composition has the following nutrients: B vitamins (cyanocobalamin 2-1,000 ug, thiamin 1-1,000 mg, niacin 15-2,000 mg, pyridoxine 1-1,000 mg, Pantothenate 5-150 mg, folic acid 400-40,000 ug), alpha-tocopherol 10-800 mg, ascorbic acid 50-10,000 mg, calcium 20-2,000 mg, vitamin A 200-10,000 ug, alpha-lipoic acid 100-1,000 mg, N-acetyl cysteine 100-3,000 mg, L-carnosine 100-9,000 mg, tyrosine 100-9,000 mg, vanillin 10-100 mg, phosphatidylserine 10-800 mg, resveratrol 10-50 mg, dehydroepiandrosterone 1-50 mg, and melatonin 0.1-3 mg, all of which have been individually used experimentally or clinically for relieving stress, preventing and treating age- and stress-related disorders and diseases but no combination of these compounds has been used. Many embodiments also contain at least one adjunct ingredient such as coenzyme Q 10-200 mg, acetyl-L-carnitine 100-2,000 mg, choline 50-1,000 mg, and creatine 100-2,000 mg.

A skin care composition is provided which preferably comprises a mixture of N-acetylcysteine and L-carnosine in combination with a cosmetic, dermatological or pharmaceutically acceptable carrier therefor. The composition may optionally be provided as a sunscreen formulation, and provides a method for the prevention, amelioration or treatment of pathological conditions of the skin, including, but not limited to, intrinsic or chronological aging, or aging due to sun damage (photoaging), and which conditions are caused by, or exacerbated by, oxidative stress, carbonyl stress, or a combination of both.

A dietary supplement composition for enhancement of the insulin sensitivity is provided, which includes thiamin, chromium, alpha-lipoic acid, L-carnosine, and vanadium. The supplement composition further includes cinnamon bark and banana leaf extract (corosolic acid) for an extra strength formula. Alternatively, the supplement composition includes a herbal blend which includes boswellic acid, gymnema sylvestre leaf extract, and bitter melon extract. Further provided is a method of using the supplement composition for enhancing an individual's insulin sensitivity.

A method for synthesizing L-carnosine belongs to the organic synthesis technical field. The method is as follows: beta-alanine is dissolved in non-polar solvent to be reacted with phthalic anhydride under the catalysis of organic amine, and then phthaloyl-beta-alanine is obtained through water recrystallization; the phthaloyl-beta-alanine is dissolved in solvent to synthesize phthaloyl-beta-alanyl chloride through the chlorinated reagent of acyl chloride phthaloyl-beta-alanine; L-histidine is reacted with hexamethyl disilazane or trimethylchlorosilane to obtain L-histidine trimethylsilane protector; the protector is reacted with the phthaloyl-beta-alanyl chloride to obtain hydrochloride product with the protecting group divested by water, and then neutralization product is obtained by the hydrochloride product obtained through the neutralization condensation reaction of alkaline reagent, thereby obtaining L-carnosine crude product through the hydrazinolysis of the neutralization product by hydrazine hydrate; and the crude product is purified to obtain L-carnosine finished product. The method has low raw material consumption, short reaction procedure and high yield; moreover, the quality of synthesized L-carnosine can meet the requirements of industrial production.

The invention discloses a synthetic method of L-carnosine, and belongs to the technical field of organic matter synthesis. The method comprises the following steps: dissolving 3-chloropropionic acid in an organic solvent, and converting the 3-chloropropionic acid by a chloroformylation reagent to form corresponding 3-chloropropionyl chloride; condensing trimethylsilane protected L-histidine and the 3-chloropropionyl chloride to obtain a corresponding amide product; removing protection agents by using water or an alkaline solution to obtain an intermediate; carrying out aminolysis on the intermediate to obtain crude L-carnosine; and purifying the crude L-carnosine to obtain finished L-carnosine. The synthetic method has the advantages of low raw material consumption, short reaction steps, few wastes, high yield, obtaining of high-quality L-carnosine free from hydrazine, and meeting of industrial production demands.

The invention relates to a preparation method of L-carnosine zinc, which belongs to the technical field of preparation of pharmaceutical intermediates. The preparation method comprises the steps of dissolving L-carnosine in water for forming water solution of the L-carnosine, leading the water solution of the L-carnosine to be reacted with ion exchange resin for absorbing the L-carnosine on the resin for activation, adopting the water solution of zinc salt for eluting the resin absorbed with the L-carnosine, combining zinc ions in eluent with the activated L-carnosine molecules for forming L-carnosine zinc, precipitating from the water and carrying out solid-liquid separation on the eluent, thereby obtaining the L-carnosine zinc. The preparation method has the advantages that the technicalscheme for preparing the L-carnosine zinc has good yield, the zinc content in a product is not less than 22.0%, the optical rotation is not less than plus 8.0 degrees, and the quality is stable; andthe technical scheme does not use an organic solvent, the used ion exchange resin is recycled, and the main raw material of the L-carnosine is completely reacted, thereby improving the utilization rate of raw materials, reducing the preparation cost and meeting the industrial production requirements.

The invention discloses a preparing method for N-acetyl-L-carnosine and belongs to the technical field of medical intermediates. The method comprises the steps that beta-alanine is subjected to ammonia acetylization to obtain N-acetyl-beta-alanine; N-acetyl-beta-alanine and L-histidine are condensed to obtain N-acetyl-beta-carnosine. N-acetyl-beta-alanine and an acylation reagent react in a non-polar solvent to obtain N-acetyl-beta-alanyl chloride, and L-histidine and organosilane react under the catalyzing of acid to obtain organosilane-protected L-histidine; then organosilane-protected L-histidine and N-acetyl-beta-alanyl chloride are condensed to obtain organosilane-protected N-acetyl-L carnosine, a polar solvent is added to remove protecting groups, and N-acetyl-L-carnosine is obtained through separation and purification. Or, N-acetyl-beta-alanine and L-histidine are condensed under the action of a condensing agent to obatin N-acetyl-L-carnosine.

The invention discloses a synthesis method for L-Carnosine. In the prior art, the imidazole ring of the L-Histidine can take part in the reaction and has side-reactions, which decreases the yields of the products and makes the L-Carnosine assume the toxicity at the same time. In this invention, the o-phthalic anhydride is reacted with beta-alanine to produce the o-phthalic beta-alanine, the o-phthalic beta-alanine is made an acyl chlorination by the chloro-substitution reagent to synthesize into the o-phthalic beta-alanyl chloride; L-Carnosine is reacted with the edittrialkyl chlorosilane or hexamethyldisilazane to produce the trialky silane protection compound which is condensed with the o-phthalic beta-alanyl chloride to produce the hydrochloride; the hydrochloride is made a de-protection by the absolute alcohol, and then is neutralized by the basic solution to produce a product of neutralization; the hydrazine hydrate is used for a hydrazinolysis to the product of neutralization, then the L-Carnosine is precipitated from the absolute alcohol. The invention protects the imidazole ring on the L-Carnosine, avoids that the imidazole ring on the L-Carnosine makes side-reactions with other substances so as to obtain the pure L-Histidine, has low side-effects and high total yields as well as contents.

The invention discloses a method for preparing polaprezinc, and belongs to the technical field of pharmacy. The method comprises the following steps: 1, preparing a solution to 2-cyano acetyl chlorideand dichloromethane, dropwise adding the solution to a trimethylsilyl-protected dichloromethane solution of L-histidine, and adding imidazole to obtain (s)-2-cyanoacetamido-3-(1H-imidazole-4-yl)propionic acid; carrying out a catalytic hydrogenation reaction to obtain crude L-carnosine; carryin gout alkaline macro-porous ion exchange resin adsorption, flushing the adsorbed L-carnosine with an aqueous solution of alkali metal acetate, and carrying out spray drying to obtain an alkali metal salt solid of the L-carnosine; and dissolving the alkali metal salt solid of the L-carnosine in methanol,adding a methanol solution of zinc acetate, and beating and refining the obtained solution by using a methanol-water solution to obtain refined polaprezinc. The method allows highly-pure L-carnosine sodium and polaprezinc to be obtained, and allows the time of the refining and drying process of the polaprezinc to be short, and the refined polaprezinc has the advantages of easiness in crushing, high yield and low cost, so the method is suitable for industrial production.

The present invention discloses an L-carnosine preparation method, which is characterized in that N-BOC-beta-alanine and L-histidine methyl ester hydrochloride are subjected to an aminolysis reaction under strong base catalysis, and the acid is subjected to deprotection to obtain the product, wherein the total yield of the two-step process is up to 72-75%. The preparation method has characteristics of significant process shortening, simple operation, high yield, good purity, low cost and the like, and is a process suitable for industrial production.

Disclosed herein is a composition comprising a liposome, a cardiolipin, and at least one antioxidant, wherein the composition is useful for improving, maintaining, or restoring a mitochondrial membrane and / or mitochondrial function. In one example, the liposome is primarily composed of phosphatidylcholine, the cardiolipin is tetraoleoyl-cardiolipin, and the at least one antioxidant is methylgentisate, l-carnosine, or both. The composition may be topically administered, orally administered, or parenterally administered, for example administered by injection. If topically administered, the composition may be administered for example, as a cream, lotion, gel, paste, spray, tonic, or other suitable form and may contain various additives, for example, one or more of an emollient, a humectant, a penetration enhancer, a vitamin, a fragrance, a pigment, and a moisturizer.

The invention relates to a composite solution of sodium hyaluronate and application of the composite solution for reducing skin wrinkles. The composite solution comprises sodium hyaluronate, L-carnosine, amino acid and a water soluble vitamin as main components, including 1.000 to 5.000mg / mL of sodium hyaluronate, 1.000 to 2.000mg / mL of L-carnosine, 0.100 to 0.500mg / mL of amino acid and 0.001 to 0.005mg / mL of water soluble vitamin B2, and the pH value of the composite solution is 7 to 7.2. By adopting the composite solution, extracellular matrixes can be compensated to skin, synthesis of skin collagen protein can be promoted, and a function of reducing wrinkles can be achieved. Clinical tests on the composite solution show that the composite solution can be used for reducing winkles, and is good in safety and free of conspicuous complication.

The invention relates to an injection (intracutaneous injection) for correcting skin wrinkles and a preparation method thereof. The injection is prepared from the following components by mass: 3-6g / L of high molecular polysaccharide, 50-200mg / L of amino acid, 2-10mg / L of water-soluble vitamin and 2-5g / L of L-carnosine. A sample prepared according to the formula is capable of supplying extracellular matrix to skin and promoting synthesis of spontaneous collagen of skin. The injection has the function of a traditional filling material for supplying lost extracellular matrix, is capable of promoting synthesis of collagen and solving the problem of wrinkled and loose skin caused be senility. Furthermore, compared with a conventional filling material, the injection is lower in viscosity and is more suitable for intracutaneous injection. According to the injection prepared by the method, the problem of sodium hyaluronate and collagen degradation of skin in the aging process can be solved by means of the intracutaneous injection, and an anti-wrinkle and skin-firming effect can be achieved.

The present invention relates to improved chemical synthesis process of N-acetyl-L-carnosine. The main material L-carnosine is acetylated with acetyl chloride as the acetylating agent through reaction in alkaline water solution at proper temperature and pH value in the presence of sodium acetate as acid binding agent. The process has N-acetyl-L-carnosine yield of 77.5-82.0 % and no racemisation.

The invention belongs to the technical field of bioengineering, and relates to carnosine hydrolase and a mutant thereof, a recombinant expression vector containing the enzyme and a mutant gene, and arecombinant expression transformant, a preparation method of the recombinant enzyme, a method for immobilizing the recombinant enzyme, and a method for preparing levo form L-carnosine by using the recombinant enzyme through reverse hydrolysis reaction. Compared with the prior art, the carnosine hydrolase disclosed by the invention is high in activity and high in thermal stability, and the enzyme is used for catalyzing beta-alanine and L-histidine to prepare L-carnosine through direct condensation, so that the steps of protection and deprotection in a conventional chemical method of synthesizing peptides are avoided; the process is simple, and the conditions are mild and environmentally friendly. Therefore, the carnosine hydrolase has an extremely good application prospect in industrial production of the L-carnosine.

The invention relates to an improved preparation method of L-carnosine. The preparation method comprises the following steps: taking water as reaction solvent at the reaction temperature from 0 to 30DEG C, adjusting the pH value of the reaction solution of L-histidine showed in a formula (II) and tetrahydro-1, 3-thiazine-2, 6-diketone showed as a formula (III) to be 8.2-9.2 by trimethylamine andcarrying out the reaction, and carrying out the separating treatment to the reaction solution after the reaction is finished to obtain the L-carnosine shown in a formula (I), wherein the mass ratio between the L-histidine and the tetrahydro-1, 3-thiazine-2,6-diketone is 1: 1.01 to 1.27. The invention provides a novel L-carnosine synthesis process which has simple process, easily-obtained raw materials, low production cost and less environment pollution, and is beneficial to industrial production.

The invention discloses a method of synthesizing L-carnosine using L-amino acid ligase by a one-step method. The method comprises the steps of taking beta-alanine, L-histidine, ATP (adenosine triphosphate) and polyphosphate as raw materials, and MgCl2 as an activator, adding L-amino acid ligase and polyphosphate kinase, and synthesizing L-carnosine through an enzymatic reaction and coupled coupleunder conditions of a pH of 6.5-8.5 and a temperature of 30-45 DEG C. According to the method, a sequence optimized L-amino acid ligase gene is successfully expressed in escherichia coli, and beta alanine and L-histidine can be catalyzed to synthesize L-carnosine at one step. A substrate is not required to be methylated or subjected to group protection; ATP required by a catalytic reaction is continuously regenerated by allowing polyphosphate kinase to catalyze phosphorylation of ADP (adenosine diphosphate); cyclic regeneration of ATP can be achieved only by consuming a small amount of ADP; and a renewable raw material, sodium hexametaphosphate, is wide in source, low in cost, simple to operate and easy in large-scale production.

The invention discloses a production method of carnosine, which is characterized by comprising the following steps of: synthesizing N-acetyl cyanide-L-histidine through ammonolysis reaction by using L-histidine and ethyl cyanoacetate as raw materials and sodium alcoholate as a catalyst; and then obtaining L-carnosine through the intermediate by using stronger ammonia water and alcohol as a solvent in a high-pressure reaction kettle and using a Pd-Rh / C composite catalyst for catalytic hydrogenation under the protection of nitrogen, wherein the overall yield of two-step reaction achieves 72 percent. The invention has simple process, easy operation, low cost, and the like and is very suitable for industrialized production.

An composition for application to a disordered tissue, the composition including an antipathogenic agent, a healing agent and an optional soothing agent. The antipathogenic agent can include cineole, and the healing agent can include an amino acid such as L-arginine or L-carnosine. The cineole can be of dual function, for example, it can operate as an antipathogenic agent, and also operate to enhance penetration of at least one of the antipathogenic agent, healing agent and / or soothing agent into the disordered tissue, which can be skin of a subject. The disordered tissue can be afflicted by an infection, for example, a Type I herpes infection, and can be in the form of a cold sore. The composition can be in a form, such as a cream or a lip balm, adapted for topical application to the disordered tissue. A method for treating the disordered tissue with the composition is also provided.

The invention belongs to the technical field of beautifying, and mainly relates to a stem cell composition containing an adipose derived stem cell exogenous protein extract, a preparation method of the extract and application thereof. The composition includes the components of adipose derived stem cell exogenous protein extract, milk thistle extract, tea polyphenol, L-carnosine and evening primrose extract. The preparation method of the composition comprises the following steps: a) collecting an adipose derived stem cell culture supernatant and conducting ultra high speed centrifuging to collect a stem cell exogenous protein; b) mixing the milk thistle extract, the tea polyphenol, the L-carnosine, the evening primrose extract and the adipose derived stem cell exogenous protein extract. Thecomposition is simple in component, the stem cell protein used is derived from auto adipose derived stem cells, the material is not limited, no rejection reaction exists, and the anti-glycation effect has broad application prospects in anti-aging cosmetics.

The invention discloses an oxidation resisting dipeptide medicament intercalated houghite and a preparation method thereof, belonging to the technical field of dipeptide medicaments. The chemical formula thereof is: (Mg<2+>)1-x(Al<3+>)x(OH)a(L-Carnosine)b.mH2O. The preparation method thereof comprises the coprecipitation method and the ion exchange method; wherein the crystallization phase of the coprecipitation method and the reaction process of the ion exchange method both adopt the microwave-hydrothermal method. The invention has the advantages that: the microwave-hydrothermal method is adopted in the preparation process of the carnosine intercalated houghite, thus greatly shortening the crystallization period of the coprecipitation method and the reaction period of ion exchange method; the prepared dipeptide medicaments--- carnosine intercalated houghite composite material uses the space limited domain action of the houghite and the interaction between the subject and the object, thus having better thermal stability and certain slow release performance under normal conditions and being favorable for the storage thereof and the development of new dosing route.

A prepartion method of N-acetyl-L-carnosine, relates to pharmaceutical intermediates synthesis technology field. Dissolving p-nitrophenol into organic solvent, reacting with acetyl chloride to obtain active ester solution at the presence of acid-binding agent; dissolving the L-carnosine and protection agent into water to obtain aqueous solution, then adding the avtive ester solution into the aqueous solution for reacting, and when the reaction is finished, demixing, decoloring the water layer and decompressing condensing to pulpous state, then mixing the condensed residue with acetate acid and adding into polar solvent for stirring, cold separating and drying to obtain the N-acetyl-L-carnosine. The advantages are: the molar yield is more than 90%, the chemical purity is more than 90%, therefore high yield and ideal purity is suitable for industrial production.

The invention discloses a method for synthesizing L-carnosine by using a microorganism enzyme method and belongs to the technical field of carnosine preparation. The method comprises the following steps: directly putting an organic solvent which is used for activating activity of a microorganism enzyme and is used as a permeation agent into an L-carnosine reaction system with the microorganism enzyme in a reaction container provided with a stirring device, directly adding divalent metal ions for activating the activity of the microorganism enzyme, carrying out a reaction under stirring of the stirring device, and carrying out centrifugal separation after the reaction is completed, thereby obtaining L-carnosine. The microorganism enzyme can be activated, the permeation barrier of extraneous coats of cells can be reduced, later crystal separation can be facilitated, the synthesis cost can be lowered, the reaction speed can be remarkably increased, and the productivity can be improved.

The invention discloses a preparation method of non-hydrazine L-carnosine, and belongs to the technical field of organic synthesis. The method comprises the following steps: reacting anhydrous o-benzoyl diformyl-L-carnosine with 5-10 times of weight of low fat serotonin at the temperature of 20-35 DEG C for 8-12 hours to remove a protecting group; and after reaction is finished, distilling to remove the low fat serotonin, adding a crystallization solvent for crystallization, and filtering, washing and drying to obtain the product, wherein the low fat serotonin is alkyl primary amine of C3-C8.The method has the advantages that the cost is low, energy consumption is also low, the operability is high, the obtained product L-carnosine does not contain toxic substance hydrazine, and thus, thesafety of the L-carnosine is fully guaranteed.

The present invention provides combined use of a carnosinase inhibitor with L-carnosine and its related substance, and a composition containing the same, which are useful for treatment or prevention of various diseases, improvement of health conditions, improvement of exercise ability, improvement of skin health, prevention of the side effects of alcohol drinking and the like in mammals including human.

The invention discloses a method for efficiently catalyzing and synthesizing L-carnosine in a whole cell. The method comprises the following steps of 1) constructing a recombinant vector containing anamino acid fatty acyltransferase gene with a nucleotide sequence shown in SEQ ID NO.1, wherein the amino acid sequence encoded by the gene is shown in SEQ ID NO. 2; 2) transferring a gene with a nucleotide sequence shown in SEQ ID NO.1 to bacterium coli to obtain recombinant bacterium coli; 3) dissolving a substrate beta-alanine methyl ester and L-histidine in a buffer solution, wherein the pH is7.5-9.5; 4) adding the recombinant bacterium coli to the buffer solution for reaction, wherein the reaction temperature is 25-42 DEG C. A recombinant plasmid and recombinant engineered bacteria of the gene directly catalyze and synthesize the L-carnosine in the catalytic system, thereby improving the conversion rate of the L-carnosine, and the problems of the complicated separation and purification of an enzyme and the high cost of an aminopeptidase catalytic process are solved.

The invention relates to a preparation method of L-carnosine. According to the invention, phthaloyl-L-carnosine and organic amine are heated in a solvent, such that protective groups are removed and a product is precipitated; filtering, solvent washing, and drying are carried out, such that an L-carnosine product is obtained; or concentration, crystallization, filtering and drying are carried out, such that a hydrazine-free L-carnosine product is obtained. The method provided by the invention has the advantages of simple method, low cost, and high operability. No toxic compound hydrazine exists in the obtained carnosine product. The quality of the obtained product can be sufficiently ensured, and the health safety of the product is shown.

A homoeopathic formula and method for treating celiac disease. The formula includes the ingredients of L-Carnosine, Aronia Berry, Vitamin D3, Folic Acid, RNA, DNA, Pulsitilla, and Lycopodium Clavatium which are mixed with brown rice flour as an anti clumping agent prior to encapsulating the formula in standard gel caps. The preferred dosage for the treatment of celiac disease is to administer 6 capsules of the preferred formulation to the patient orally each day for 30 consecutive days.

The invention discloses a method for synthetizing L-carnosine by a one-step method and truncated L-carnosine synthetase. According to the method, beta-alanine, L-histidine, ATP and polyphosphate are used as raw materials, MgCl2 is used as an activator, L-carnosine synthetase and polyphosphate kinase are added, and through an enzymatic reaction under the condition of pH being 6.5-8.5 and the temperature being 30-45 DEG C, the carnosine is coupled, catalyzed and synthetized. According to the method disclosed by the invention, escherichia coli is used for respectively expressing the truncated L-carnosine synthetase (of which the amino acid sequence is as shown in SEQID No.3) and the polyphosphate kinase, through purification, mixing is performed, a dual-enzyme coupling system is formed, and the L-carnosine synthetase in truncated expression can catalyze the L-carnosine synthetized from beta-alanine and L-histidine; besides, accompanied with ATP dephosphorylation, ADP is formed, the polyphosphate kinase catalyzes polyphosphate transphosphorylation groups, and ATP is formed for the ADP, so that circulation and regeneration of the ATP can be realized; and through the dual-enzyme couplingsystem, a reaction is performed under the appropriate reaction condition, so that carnosine is obtained. The method disclosed by the invention has the advantages that the raw materials are low in cost, the enzymatic conversion time is short, the operation is simple and convenient, and the production cost is low.