82 results about "Corosolic acid" patented technology

This invention provides an improved process for producing corosolic acid starts from ursolic acid, which occurs in plants in relatively large amounts, the method comprising the steps of oxidizing the hydroxyl group at 3-position and utilizing the carbonyl group at 3-position to introduce a hydroxyl group stereospecifically into the 3-oxoursolic acid at the 2α-position adjacent to the hydroxyl group at 3-position.

A dietary supplement composition for enhancement of the insulin sensitivity is provided, which includes thiamin, chromium, alpha-lipoic acid, L-carnosine, and vanadium. The supplement composition further includes cinnamon bark and banana leaf extract (corosolic acid) for an extra strength formula. Alternatively, the supplement composition includes a herbal blend which includes boswellic acid, gymnema sylvestre leaf extract, and bitter melon extract. Further provided is a method of using the supplement composition for enhancing an individual's insulin sensitivity.

Disclosed are preparing processes for industrialized application of Corosolic acid with blood sugar lowering, weight reducing, anti-tumor and anti-inflammatory actions and maslinic acid with anti-tumor, anti-virus and cardiovascular diseases resistant actions. The processes include respectively preparing Corosolic acid and maslinic acid using 3-carbonyl ursolic acid ester and 3-carbonyl oleanolic acid ester by acid catalysed enolization esterification reaction, hydroboration oxidation reaction, catalytic hydrogenolysis reaction and haloidlysis reaction.

The invention provides a method for extracting and preparing corosolic acid from plants, which comprises the steps of poaching and removing water-soluble substances, extracting basic organic solvents of total triterpene acids and enriching and purifying macroporous absorption resin of corosolic acid. Plants containing corosolic acid are adopted as raw material and the water-soluble substances are removed after boiled by pure water; regarding filter residue 30 to 60 percent of basic ethanol or methanol water solution is adopted to extract total triterpene acids and go through settling separation under the condition that pH is 4.5 to 7.5 to obtain crude products of total triterpene acids which are fully dissolved by 85 to 100 percent of ethanol or methanol and undissolved substances are removed; filtered liquor goes through the absorption and elution of macroporous absorption resin; the final concentration of ethanol or methanol in the elution liquor of corosolic acid is adjusted to be less than or equal to 50 percent and pH value thereof is adjusted to be less than or equal to 7.0; corosolic acid is obtained through settling separation under the condition. The method has simple preparation technique, high separation and purification efficiency, easy industrial production, low production cost and relatively great generalization.

The present invention provides a method of separating and preparing corosolic acid from loquat leaves, which includes the separation and preparation of the total triterpene acid loquat leaf, the purification of the resin concentration of the corosolic acid, the crystallization and purification of the corosolic acid, etc. In the preparation method, the loquat leaves are used as a material, and the total triterpene acid loquat leaf is extracted by applying the aqueous solution of the hydrophilic organic solvent; under the alkaline condition and before cooling, impurities are removed by filtration, and activated carbon is decolored; extracting solution applies macroporous absorptive resin concentration to purify the corosolic acid, and the crude corosolic acid is further crystallized and recrystallized by applying the hydrophilic organic solvent, so that the high-purity corosolic acid crystal is prepared. By measurement, the purity of the white corosolic acid crystal is larger than or equal to ninety eight percent. The preparation technique of the present invention has the advantages of simple technique, high efficiency of separation and purification, easy industrialized production, high purity of the prepared corosolic acid and low production cost, and moreover, the preparation technique of the present invention has the advantages of high efficiency, high availability, long effect, little dosage, little toxicity, little side effect, convenient storage, convenient carrying and convenient administration, etc. of the modern Chinese medicine.

The invention provides an early insulin secretion stimulator comprising corosolic acid, etc. The early insulin secretion stimulator of the invention is capable of rapidly inducing secretion of insulin immediately after meals without inducing secretion of insulin in the absence of blood glucose increase.

The present invention provides a method of producing a triterpene composition wherein callus induced from banaba, loquat, perilla or guava is cultured with indoleacetic acid and another plant hormone, and a triterpene composition containing at least one selected from the group consisting of corosolic acid, tormentic acid, 2α,19α-dihydroxy-3-oxo-urs-12-en-28-oic acid and maslinic acid is obtained from the cultured callus.

The present invention relates to a method of producing corosolic acid by using plant cells that produce corosolic acid. More particularly, the present invention relates to a method of producing corosolic acid by using plant cell suspension cultures comprising the steps of: inducing a callus from a tissue of a plant producing corosolic acid; preparing a cell line capable of being cultured in liquid culture medium from the induced callus; culturing the cell line in a suspension culture; and isolating corosolic acid from the culture solution. The present invention has advantage of maximizing productivity by utilizing two-stage culture, treatment with inducing agent, and high cell-density culture in the suspension culture of plant cells producing corosolic acid.

The invention discloses a method for extracting corosolic acid from loguat leaf. Based on the loguat leaf as a raw material, the high pure corosolic acid is obtained by combination of ultrasonic extraction, acid precipitation, alkaline alcohol solution dissolution, macroporous resin absorption, crystallization, re-crystallization and the like. The method disclosed by the invention has the advantages of simple process, low production, high extraction efficiency and the like.

Compositions and methods for administering a diet supplement to humans are provided for the enhancement of weight loss, suppression of appetite, prevention of muscle protein catabolism, and providing antioxidants in either a daytime or nighttime formulation. Said diet supplement is comprised of at least a source of Catechins and at least 3% Corosolic Acid. The diet supplement is provided in either daytime or nighttime formulations.

The invention discloses a method for separating and preparing corosolic acid as a blood sugar reducing active ingredient from Eleutherococcus evodiaefolius. The method comprises the following steps: (1) after pulverizing the Eleutherococcus evodiaefolius, carrying out ultrasonic extraction on the pulverized Eleutherococcus evodiaefolius to obtain extracting liquid, and concentrating the extracting liquid to obtain an extract; (2) dissolving the extract in a sodium hydroxide solution, removing undissolved substances, after neutralizing the solution via acid, extracting the solution with ethyl acetate, collecting an ethyl acetate extracting solution, and concentrating a recycling solvent to obtain a crude extract; and (3) separating the crude extract by high speed counter-current chromatography, carrying out online detection by using an ultraviolet detector, collecting fraction components, concentrating under reduced pressure, crystalizing and drying to obtain the corosolic acid. The corosolic acid is prepared by the method. The method has the advantages that the extracting rate is high, an operation process is simple, energy consumption is low, the preparation method is pollution-free, the recycling rate is high, the quality is good, the activity of physiological activators is protected, and the like. Industrial amplification is facilitated.

The present invention provides a glucose tolerance improving agent containing as an active ingredient at least one triterpene selected from the group consisting of corosolic acid, an analogous compound of corosolic acid, and a pharmaceutically acceptable salt thereof.

The invention discloses triterpene-2-alpha-hydroxylase MAA45, related biological materials thereof and application of the triterpene two-bit alpha-hydroxylase MAA45 and the related biological materials in preparing maslinic acid and corosolic acid. The triterpene two-bit alpha-hydroxylase MAA45 is the following protein shown as A1), or A2) or A3), wherein A1) represents protein with an amino acid sequence being the sequence 1 in a sequence table, A2) represents protein which has the same function and is obtained by substituting or missing or adding one or more amino acid residues to the amino acid sequence shown in the sequence 1, and A3) represents fused protein obtained by connecting labels to the N terminal or / and C terminal of A1) or A2). It is proved through experiments that maslinic acid can be prepared from MAA45 with oleanolic acid as a substrate, corosolic acid can be prepared from MAA45 with ursonic acid as a substrate, MAA45 and coding genes thereof can be used for preparing maslinic acid and corosolic acid, and MAA45 can be used for catalyzing triterpene two-bit alpha-hydroxylation.

The invention relates to a preparation method for triterpene acid of loquat leaf and the function of lowering the blood glucose. The preparation method comprises that dry loquat leaves are firstly extracted by ethanol or methanol with certain concentration, reclaimed in ethanol with low condensation for sedimentation, and centrifuged, precipitated and refluxing-decolorized with diatomite or done with chromatography with siliceous earth column, and then eluted with ethanol and reclaimed in condition of decompression and dried to obtain the triterpene acid of loquat leaf. Total content of ursolic acid, oleanolic acid, maslinic acid, corosolic acid and tormentic acid in the triterpene acid reaches more than 50 percent (by weight), wherein, the content of the tormentic acid is to be 10-30 percent, the maslinic acid 4-20 percent, the corosolic acid 5-20 percent, the oleanolic acid 1-10 percent and the ursolic acid 5-20 percent. The triterpene acid of loquat leaf prepared by the invention can be used for curing diabetes mellitus or preparing pharmaceutic preparations used for treating diabetes mellitus and diabetic complication.

This invention provides an improved process for producing corosolic acid starts from ursolic acid, which occurs in plants in relatively large amounts, the method comprising the steps of oxidizing the hydroxyl group at 3-position and utilizing the carbonyl group at 3-position to introduce a hydroxyl group stereospecifically into the 3-oxoursolic acid at the 2α-position adjacent to the hydroxyl group at 3-position.

The invention relates to the technical field of medicines, in particular to new application of corosolic acid. The corosolic acid is scientifically named as 2alpha-hydroxyursolic acid, and is a natural product existing in multiple medicinal and edible plants of banaba leaf, potentilla chinensis, loguat leaf, shine skin papaya, pomegranate extract and the like. The corosolic acid has remarkable antidepression activity without side and toxic effects, and can be used for preparing the antidepressant drugs.

The invention belongs to the technical field of beverage processing, in particular to nutrient health care rose-grape vinegar. The nutrient health care rose-grape vinegar is prepared from the following raw materials: grapes, fresh grape leaves, grape roots, fresh rose, sugar cane juice, winter bamboo shoots, fresh spinach, fresh orange peel, a rhizoma bletillae extract, a piperis longi extract, propylene glycol alginate, chrysanthemum flavone, corosolic acid and bacillus aceticus. The nutrient health care rose-grape vinegar is uniform in color and luster, is light red in color, is stable in vinegar body, is free from lamination and precipitated substances, has strong and coordinated fragrances of grape, rose and vinegar, mild and proper sourness and sweetness, and soft, mellow, fine and smooth mouthfeel, is free from stimulation, and has good treatment effects for patients suffering from skin cracking and peeling, long-term constipation, high blood fat, high blood pressure and anemia.

The invention provides a preparation method of corosolic acid, comprising the following steps: 1) using ursolic acid as a raw material, and oxidizing the raw material by an oxidant to obtain 3-carbonyl ursolic acid; 2) reacting the 3-carbonyl ursolic acid obtained from the step 1) and lead tetraacetate, and introducing acetoxy; and 3) reducing acetoxy of 2-acetoxy-3-carbonyl ursolic acid obtained in step 2) to obtain corosolic acid, and purifying the obtained corosolic acid. The reaction yield of the invention is over 34%, and the invention shortens the technical route and reduces the intermediate treating process, greatly reduces cost and provides favorable conditions for producing the corosolic acid.

The invention discloses an antibacterial active packaging material and a preparation technology thereof. The packaging material is composed of a base material and an antibacterial agent, wherein the antibacterial agent is prepared from the following components: naringin, monopotassium glycyrrhizinate, rosa laevigate michx brown, beta-apo-8'-carotenealdehyde, mesoporous carbon, corosolic acid and compound essential oil; the base material is prepared from starch, bamboo powder, vermiculite powder, bauxite, polylactic acid, polyethylene glycol, modified coconut fibers, ammonium persulfate-sodiumhydrogen sulfite and ethanol. The packaging material prepared by the preparation technology has wide antibacterial performance, and the tensile strength and the elongation at break have a relatively excellent level, so that the antibacterial agent has relatively good adaptability with the base material. Moreover, raw materials of the packaging material are green and safe; the antibacterial performance lasting time can reach 30 months or longer and the antibacterial active packaging material has a very good application prospect.

The invention belongs to the field of medicine, and relates to a drug for treating acute lung injury and acute respiratory distress syndrome, wherein the active components of the drug is ganolucidic acid A or corosolic acid or a composition of ganolucidic acid A and corosolic acid, and the dosage form of the drug is one selected from an oral liquid preparation, a pulmonary inhalation preparation and an injection. According to the present invention, the drug can reduce the pulmonary edema of acute lung injury and acute respiratory distress syndrome, reduce lung hyaline membrane formation and other lung lesions, and easily improve the symptoms of acute lung injury and acute respiratory distress syndrome.

Disclosed are combinations of soy protein, chromium and free leucine in amounts effective to inhibit the loss of lean body mass of a subject under conditions of caloric restriction. The soy protein, chromium and free leucine can be administered in several products, including a powder that is mixable into a liquid beverage (such as a shake), a snack bar, and a nutritional supplement (such as one or more tablets). Some embodiments also include corosolic acid and / or a tea drink that provides an effective dose of taurine. The products may be contained in kits containing the disclosed combinations as well as methods of using the products for weight loss and the inhibition of the loss of lean body mass in a subject during weight loss. The products and methods are also useful in the improvement of lipid profiles (for example reducing total cholesterol and LDL cholesterol levels).

The present invention relates to pharmaceutical, therapeutic, and dietary compositions derived from leaf of the Lagerstroemia speciosa L. plant and the novel extraction processes used to produce these controlled blends of varying composition with respect to corosolic acid, gallotannins, ellagitannins, and valoneic acid dilactone. Embodiments of the invention include an approximately 15.5% to about 98.5% mixture of a corosolic acid rich banaba leaf extract in a tannic acid enriched base for novel combinations for pharmaceutical, therapeutic and / or dietary compositions that yield healthful benefits. Some embodiments of the invention do not include corosolic acid, or include it at a low concentration in combination particularly enriched in other compounds. Such extract compositions that do not contain corosolic acid, are the product of extraction process that yield controllably increased ratios of gallotannins, ellagitannins, and valoneic acid dilactone. Such compositions, both those containing corosolic acid and those lacking corosolic acid are efficacious in effecting control of blood glucose levels, and can be further enhanced in their efficacy by post production and formulation strategies that utilize nanotechnological approaches, targeted deliver enteric coatings, and specialized microenacapsulations. With respect to the individual compounds of these extract compositions, such compounds show combined effects that are both additive and synergistic regarding improved glucose cellular uptake, reduction in blood glucose, insulin efficiency and the simultaneous reduction in assimilation of sugars and starches, and weight loss. Further, additive and synergistic effects are a potential benefit when these compositions are combined with other second therapeutic agents.

The invention discloses a pharmaceutical composition for treating neurodermatitis and a preparation method thereof. The pharmaceutical composition of the invention is composed of ephedrine, japonica, prolactin, inulalide, telegalic acid, and corosolic acid It is a raw material medicine prepared in proportion, and can be made into various dosage forms according to conventional preparation techniques, and has remarkable curative effect in treating neurodermatitis.

The invention belongs to the field of natural organic chemistry, and relates to a method for purifying corosolic acid in globeflower by using a microwave extraction-macroporous resin combined technology. The method is characterized in that: the purpose of the method is to overcome disadvantages of relatively low extraction rate, low extraction purity and the like of the conventional technology, and to provide a method for purifying corosolic acid in globeflower by using a microwave extraction-macroporous resin combined technology. The method has the following advantages that: the microwave extraction is adopted, such that advantages of low extraction temperature, high efficiency, short extraction time, and the like are provided, the corosolic acid extraction rate is substantially improved, and the extraction time is shortened; the macroporous resin is adopted to adsorb the corosolic acid, such that the good adsorption effect on the corosolic acid is provided; and an ethanol water solution is adopted to extract corosolic acid, such that the dissolving effect is good, the corosolic acid consumption is reduced, and the yield is increased.

The invention relates to the technical field of medicine, in particular to the application of corosolic acid in preparation of antineoplastic medicine; and when being combined with pharmaceutically acceptable supplementary material, the corosolic acid can be prepared into various oral, injection and external preparations and has better development and application prospect. On the basis of discovering that the corosolic acid has stronger antineoplastic action, the inventor further explains the mechanism of the antineoplastic action of the corosolic acid, thus retarding cancer cells at S period and inducing cell apoptosis mediation.

The invention belongs to the technical field of medical technology and nano-medicine and relates to a corosolic acid liposome as well as a preparation method and application thereof. For the liposome,corosolic acid is used for replacing cholesterol as a liposome membrane component and is combined with phospholipid to prepare the corosolic acid liposome with high drug loading capacity and good stability, and the liposome has dual effects of a liposome membrane material and a therapeutic drug at the same time. The liposome can solve the problems of poor absorption, low bioavailability, difficulty in effective delivery and the like of the corosolic acid and is capable of obviously improving the curative effects of reducing sugar and resisting inflammation and tumors of the corosolic acid. The liposome can load various antitumor drugs for antitumor therapy according to the needs so as to realize multidrug targeting co-delivery, efficacy enhancement and toxicity reduction, avoids multidrugresistance and has the synergistic anti-tumor effect, wherein the anticancer effect and the safety of prepared doxorubicin hydrochloride-corosolic acid liposome are obviously superior to the anticancer effect and the safety of doxorubicin hydrochloride lipidosome injection on the market.

The invention discloses a production method for extracting and separating corosolic acid and ursolic acid from loquat leaf. The production method comprises the following steps: crushing loquat leaf, extracting the crushed loquat leaf for three times with ethyl alcohol in a heating reflux way; mixing the extracted liquids obtained after all the tree times of extraction, concentrating for recovering alcohol till all alcohols are removed, then standing still, and filtering to obtain a precipitate; preparing a sodium hydroxide solution with the pH of 11; dissolving the precipitate in an aqueous solution, standing, and filtering to obtain a precipitate and a supernate; adsorbing the supernate with macroporous adsorption resin, precipitating and drying, thus obtaining ursolic acid; after the adsorption is finished, flushing impurities with water; eluting the resin with ethyl alcohol, collecting eluent, and concentrating for recovering alcohol till all alcohols are removed, standing and cooling until precipitate and supernate are separated; carrying out suction filtration to take a precipitate; and drying to obtain corosolic acid. By the production method disclosed by the invention, the byproduct ursolic acid can be obtained directly and unreasonable use of raw material is reduced; the product content and the product yield can be improved effectively due to use of the macroporous adsorption resin and the processes of activated carbon decoloration and organic solvent decoloration are omitted.

The invention relates to a lipid nanoparticle system based on corosolic acid or analogues thereof as well as a preparation method and application of the lipid nanoparticle system. The lipid nanoparticles provided by the invention are prepared from corosolic acid or analogues thereof and a lipid material, the corosolic acid analogues comprise ursolic acid and oleanolic acid, and the lipid material consists of ionizable cationic lipid, neutral phospholipid, PEGylated phospholipid and cholesterol-containing or cholesterol-free materials. The molar ratio of corosolic acid or analogues thereof to the liposome material is (1: 9)-(1: 1). Compared with the prior art, the lipid nanoparticles based on corosolic acid or analogues thereof provided by the invention have stronger cell transmembrane and tissue infiltration capacities, are used as delivery carriers of nucleic acid molecules, can be efficiently taken by cells after entrapment of at least one nucleic acid drug, and can be effectively absorbed by the cells through endosome escape capacity. The prevention and treatment effects of the nucleic acid medicines are obviously improved.

With an object of discovering a substance having a testosterone 5α-reductase inhibiting action, a hair papilla cell growth promoting action, a fibroblast growth factor-7 production promoting action, a vascular endothelial growth factor production promoting action or a bone morphogenetic protein-2 production promoting action, and of providing a hair care product having this substance blended therein, and a hair growth tonic, a testosterone 5α-reductase inhibitor, a hair papilla cell growth promoter, a fibroblast growth factor-7 production promoter, a vascular endothelial growth factor production promoter and a bone morphogenetic protein-2 production promoter each having this substance as an active ingredient thereof, a hair care product of the present invention is made to contain corosolic acid therein, and each of a hair growth tonic, a testosterone 5α-reductase inhibitor, a hair papilla cell growth promoter, a fibroblast growth factor-7 production promoter, a vascular endothelial growth factor production promoter and a bone morphogenetic protein-2 production promoter of the present invention is made to contain corosolic acid as an active ingredient thereof.