Preparing method of loquat leaf total triterpenic acid and antidiabetic use thereof

A technology for total triterpene acids and loquat leaves is applied in the field of extraction and preparation of total triterpenoid acids, and can solve problems such as adverse reactions of western medicines, adverse reactions, lactic acidosis and the like

Inactive Publication Date: 2008-07-23
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging from the current clinically used drugs, Western medicines have certain limitations and adverse reactions, even very serious adverse reactions, such as hypoglycemia, lactic acidosis, etc.

Method used

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  • Preparing method of loquat leaf total triterpenic acid and antidiabetic use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]Embodiment 1: dry loquat leaf 1.0kg is pulverized into coarse powder, with 80% ethanol 8L successively, 6L cold-soaks twice, each 15 days, merges soaking liquid and merges, concentrates under reduced pressure to the concentrated solution of alcohol degree about 30%, Cool, put in a centrifuge to settle and centrifuge (4000 rpm, 15 minutes) to get 46g of precipitate, add 230ml of 95% ethanol and heat to dissolve, then add 92g of diatomaceous earth to mix the sample, get 92g of diatomite to pack the column, after loading the sample , eluted with 2000ml of 95% ethanol, the eluents were combined, the ethanol was recovered under reduced pressure, and evaporated to dryness to obtain 35g of light yellow powder, which was the total triterpene acid extract of loquat leaves. The total triterpene acid extract of loquat leaves was analyzed by HPLC, and it was 21.7% of pollentinic acid, 10.5% of masilinic acid, 13.6% of corosolic acid, 3.1% of oleanolic acid, and 10.6% of ursolic acid....

Embodiment 2

[0038] Example 2: 1.0 kg of dried loquat leaves were crushed into coarse powder, extracted twice with 80% ethanol 6L and 4L under reflux successively, the combined extracts were concentrated under reduced pressure to a concentrated solution with an alcohol degree of about 20%, cooled, and placed in a centrifuge Sedimentation and centrifugation (rotating speed 2000 rpm, 15 minutes), 41g of precipitate was obtained, 205ml of 95% ethanol was added to heat and dissolve, and 41g of diatomite was added to mix the sample, and 82g of diatomite was loaded into the column. 2000ml eluted, the eluents were combined, the ethanol was recovered under reduced pressure, and evaporated to dryness to obtain 32g of light yellow powder, which was the total triterpene acid extract of loquat leaves. The total triterpene acid extract of loquat leaves was analyzed by HPLC, and it was found that 20.9% of policinic acid, 10.1% of masilinic acid, 14.2% of corosolic acid, 3.0% of oleanolic acid, and 8.9% o...

Embodiment 3

[0039] Example 3: 1.0 kg of dried loquat leaves were crushed into coarse powder, extracted twice with 80% ethanol 6L and 4L under reflux successively, the combined extracts were concentrated under reduced pressure to a concentrated solution with an alcohol degree of about 20%, cooled, and placed in a centrifuge Sedimentation and centrifugation (2000 rpm, 15 minutes) to obtain 43g of precipitate, add 215ml of 95% ethanol and heat to dissolve, add 43g of diatomaceous earth to mix the sample, heat to reflux for decolorization, filter while hot, recover ethanol under reduced pressure, evaporate to dryness, Obtain 34g of light yellow powder, which is the total triterpene acid extract of loquat leaves. The total triterpene acid extract of loquat leaves was analyzed by HPLC, and it was 18.3% of policinic acid, 10.4% of masilinic acid, 14.7% of corosolic acid, 2.6% of oleanolic acid, and 8.9% of ursolic acid.

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Abstract

The invention relates to a preparation method for triterpene acid of loquat leaf and the function of lowering the blood glucose. The preparation method comprises that dry loquat leaves are firstly extracted by ethanol or methanol with certain concentration, reclaimed in ethanol with low condensation for sedimentation, and centrifuged, precipitated and refluxing-decolorized with diatomite or done with chromatography with siliceous earth column, and then eluted with ethanol and reclaimed in condition of decompression and dried to obtain the triterpene acid of loquat leaf. Total content of ursolic acid, oleanolic acid, maslinic acid, corosolic acid and tormentic acid in the triterpene acid reaches more than 50 percent (by weight), wherein, the content of the tormentic acid is to be 10-30 percent, the maslinic acid 4-20 percent, the corosolic acid 5-20 percent, the oleanolic acid 1-10 percent and the ursolic acid 5-20 percent. The triterpene acid of loquat leaf prepared by the invention can be used for curing diabetes mellitus or preparing pharmaceutic preparations used for treating diabetes mellitus and diabetic complication.

Description

Technical field: [0001] The invention relates to a method for extracting and preparing total triterpene acids from leaves of traditional Chinese medicine loquat and the application of the extract in lowering blood sugar. Background technique: [0002] Diabetes is a common endocrine and metabolic disease, which belongs to the category of diabetes in traditional Chinese medicine, and is the third major disease that endangers human health after cardiovascular disease and cancer. According to the World Health Organization report, there are as many as 125 million people with diabetes worldwide, and this number is expected to increase to 299 million by 2025. Due to the large population base, there are more than 30 million diabetic patients in my country, and the absolute number of patients ranks first in the world. Because diabetes has the characteristics of high prevalence rate, morbidity rate and high complication rate, it has become a kind of disease that the medical field pay...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61K31/56A61P3/10
Inventor 李维林陈剑吕寒
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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