New production method for extracting and separating corosolic acid and ursolic acid from loquat leaf

Abstract

The invention discloses a production method for extracting and separating corosolic acid and ursolic acid from loquat leaf. The production method comprises the following steps: crushing loquat leaf, extracting the crushed loquat leaf for three times with ethyl alcohol in a heating reflux way; mixing the extracted liquids obtained after all the tree times of extraction, concentrating for recovering alcohol till all alcohols are removed, then standing still, and filtering to obtain a precipitate; preparing a sodium hydroxide solution with the pH of 11; dissolving the precipitate in an aqueous solution, standing, and filtering to obtain a precipitate and a supernate; adsorbing the supernate with macroporous adsorption resin, precipitating and drying, thus obtaining ursolic acid; after the adsorption is finished, flushing impurities with water; eluting the resin with ethyl alcohol, collecting eluent, and concentrating for recovering alcohol till all alcohols are removed, standing and cooling until precipitate and supernate are separated; carrying out suction filtration to take a precipitate; and drying to obtain corosolic acid. By the production method disclosed by the invention, the byproduct ursolic acid can be obtained directly and unreasonable use of raw material is reduced; the product content and the product yield can be improved effectively due to use of the macroporous adsorption resin and the processes of activated carbon decoloration and organic solvent decoloration are omitted.

Description

technical field [0001] The invention relates to the technical field of health care products, in particular to a new production method for extracting and separating corosolic acid and ursolic acid from loquat leaves. Background technique [0002] Loquat leaf, also known as bay leaf, is the leaf of Rosaceae plant loquat. It is a kind of substance rich in a variety of triterpene acids and other broad biological activities. The most representative ones are corosolic acid, ursolic acid Fruit acid, maslinic acid and oleanolic acid, etc. The content of corosolic acid and ursolic acid is the highest. In recent years, with the research on its medicinal effect, it has been found that corosolic acid has the effects of treating diabetes, losing weight, and lowering blood pressure, and is called a new type of plant insulin. Ursolic acid has sedative, anti-inflammatory, immune-boosting, antibacterial, whitening, anti-cancer effects. These two substances are constantly being used as res...

AI Technical Summary

Problems solved by technology

[0005] 1. Since the above method can only obtain one active ingredient, the raw materials are not effectively and fully utilized, resulting in a waste of resources

[0006] 2. Silica gel column chromatography is currently only in the stage of small batch production in the laboratory, and there is no more application in large production

[0007] 3. Decolorization in the above process will cause a large loss of active ingredients

[0008] 4. Excessive application of organic solvents in the production process increases production costs

[0009] 5. Due to the flammable and explosive characteristics of organic solvents, the above method is likely to cause great potential danger to the production process