Method for extracting corosolic acid from plant

Abstract

The invention provides a method for extracting and preparing corosolic acid from plants, which comprises the steps of poaching and removing water-soluble substances, extracting basic organic solvents of total triterpene acids and enriching and purifying macroporous absorption resin of corosolic acid. Plants containing corosolic acid are adopted as raw material and the water-soluble substances are removed after boiled by pure water; regarding filter residue 30 to 60 percent of basic ethanol or methanol water solution is adopted to extract total triterpene acids and go through settling separation under the condition that pH is 4.5 to 7.5 to obtain crude products of total triterpene acids which are fully dissolved by 85 to 100 percent of ethanol or methanol and undissolved substances are removed; filtered liquor goes through the absorption and elution of macroporous absorption resin; the final concentration of ethanol or methanol in the elution liquor of corosolic acid is adjusted to be less than or equal to 50 percent and pH value thereof is adjusted to be less than or equal to 7.0; corosolic acid is obtained through settling separation under the condition. The method has simple preparation technique, high separation and purification efficiency, easy industrial production, low production cost and relatively great generalization.

Description

technical field [0001] The invention relates to a green chemical production method for extracting medicinal active ingredients from plants, and more specifically relates to an industrially-producible method for extracting corosolic acid from plants. Background technique [0002] Corosolic acid belongs to pentacyclic triterpene acid compounds and is a derivative of ursolic acid. Compared with ursolic acid, corosolic acid has an extra α-hydroxyl group on the 2-carbon, commonly known as 2α-hydroxyursolic acid. [0003] Due to the complex structure of corosolic acid and the difficulty of artificial synthesis, corosolic acid is extracted from Chinese herbal medicines or plants at home and abroad. Corosolic acid pure product is white crystalline powder, melting point 242-244 ℃, odorless, tasteless, soluble in polar organic solvents such as hot ethanol, slightly soluble in fat-soluble solvents such as chloroform and ether, insoluble in petroleum ether and in the water. [0004] ...

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Problems solved by technology

[0007] Chinese patent application 200710009556.7, Chen Jianfeng et al. reported the method for the separation and preparation of corosolic acid from loquat leaves. Under alkaline conditions, filter while hot to remove impurities and decolorize with activated carbon. Loquat leaf extract is enriched and purified with macroporous adsorption resin for corosolic acid. The viscosity of the loquat leaf extract and the high content of water-soluble impurities severely limit the adsorption capacity and selectivity of the macroporous adsorption resin for corosolic acid, resulting in a small total yield of corosolic acid and a sharply high total production cost , The prepared corosolic acid has low purity

[0008] Chen Longsheng et al. only investigated the influence of solvent extraction conditions on the extraction rate of corosolic acid (Chen Longsheng et al., Forest Products Chemistry and Industry, 2006, 26 (3), 110), but the separation and purification process of corosolic acid is not yet mature.

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