3results about How to "Avoid steric hindrance" patented technology

The application discloses a modular dual translation regulation molecule system and a construction method and application thereof, relates to the fields of synthetic biology and nucleic acid engineering. The translation regulation system is an artificially optimized single-stranded mRNA molecule, which orthogonally integrates a translation inhibition module (OFF switch) and a translation activation module (ON switch) in the same transcript. The mRNA molecule sequentially integrates the following elements: a 5' end cap structure, a 5' untranslated region, an open reading frame of a target protein, a single-copy inhibitory miRNA binding site inserted at a specific position upstream of the 3' untranslated region, a poly-A tail, an activating miRNA binding site (responsive to miR-21), and a translation inhibition element. The application realizes precise regulation by using the difference in endogenous miRNA expression abundance between target cells and non-target cells, and is safer in use.

This invention discloses an anti-Dabie Banda virus Gn protein nanobody, a bispecific antibody, and their applications, belonging to the field of biodetection technology. This invention constructs an alpaca natural nanobody library, using recombinantly expressed Gn protein as the antigen. Through multiple rounds of gradient screening using phage display technology, three nanobodies—G15, G20, and G40—that specifically bind to the Dabie Banda virus Gn protein are obtained. All three antibodies exhibit excellent antigen-binding specificity and virus-neutralizing activity; among them, G40 shows the most prominent neutralizing effect. The bispecific antibody G40-C1q, constructed based on G40, combines Gn protein and human C1q protein, achieving a synergistic effect of antiviral activity and inhibition of inflammatory damage. It can be used for the development of drugs for the prevention and treatment of Dabie Banda virus infection and for high-specificity detection reagents, providing candidate antibody drugs for the treatment of fever with thrombocytopenia syndrome, and providing a reliable tool for virus-related research, disease diagnosis, and clinical translation.

This invention relates to the field of wastewater treatment technology, specifically a method for treating high-salt organic wastewater containing polyenes and polyamines. This invention overcomes the problems of high toxicity of polyenes and polyamines, easy complexation with catalysts, and low biochemical efficiency under high-salt conditions in wastewater treatment. First, this invention utilizes copper-supported cross-linked chitosan to achieve specific complexation and sedimentation of macromolecules, significantly reducing toxicity. Then, it uses oxygen-rich vacancy titanium dioxide particle electrodes combined with ultrasound to efficiently break stubborn C-N bonds. Next, bimetallic catalytic ozone completely eliminates residual biotoxicity. Finally, iron-modified biochar provides a salt-resistant microenvironment and electron shuttle effect for salt-tolerant bacteria, achieving deep mineralization and denitrification of pollutants. This effectively solves the biochemical treatment problem under the dual stress of high salt and high toxicity, resulting in stable effluent quality.