Application of corosolic acid in preparation of antineoplastic medicine

An anti-tumor drug, corosolic acid technology, applied in the field of medicine, can solve problems such as anti-tumor effects that have not yet been seen, and achieve the effect of high safety and convenient source

Inactive Publication Date: 2010-11-17
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Current studies have found that it has good hypoglycemic acti

Method used

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  • Application of corosolic acid in preparation of antineoplastic medicine
  • Application of corosolic acid in preparation of antineoplastic medicine
  • Application of corosolic acid in preparation of antineoplastic medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 (corosolic acid is to the inhibitory action of tumor cell growth)

[0018] Five tumor cell lines were selected to test the in vitro anti-tumor activity of corosolic acid, namely liver cancer cells: PLC / PRF / 5, Hep3B, HepG2; intestinal cancer cells: SW480 and cervical cancer cells HeLa, the tumor cell lines used were preserved In the Laboratory of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, where PLC / PRF / 5, Hep3B, HepG 2 Cultured in MEM medium (Gibco) containing 10% FBS (PAA); SW480 and HeLa were cultured in RPMI 1640 medium (Gibco) containing 10% FBS (PAA); subcultured twice a week. The cell viability after corosolic acid treatment was detected by MTT assay.

[0019] Experimental method (MTT method): Take tumor cells in exponential growth phase, digest with 0.25% trypsin containing 0.05% EDTA, resuspend and count. 1 x 10 per well 4 Inoculate in 96-well culture plate. After culturing for 24 h, the supernatant was a...

Embodiment 2

[0021] Embodiment 2 (mechanism research of corosolic acid antitumor effect)

[0022] The test results of Example 1 show that corosolic acid has a significant inhibitory effect on the growth of various tumor cell lines. On this basis, the inventor takes HeLa cells as the research object to further explore the effect of corosolic acid on inhibiting the growth of tumor cells. mechanism.

[0023] 1. Effect of corosolic acid on HeLa cell cycle

[0024] Experimental method: select cells in exponential growth phase, and use 2×10 5 Seed in a 6-well plate at a density of 6 wells, culture in RPMI 1640 containing 10% FBS for 24 hours, replace with complete culture solution containing different concentrations of corosolic acid for 6 hours, 12 hours, and 18 hours respectively, collect cells, fix with 70% ethanol, and PBS After rinsing and staining with 20 μg / ml PI, they were detected by flow cytometry. The distribution of cells in each cycle was analyzed with ModFit LT V3.0 software, th...

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Abstract

The invention relates to the technical field of medicine, in particular to the application of corosolic acid in preparation of antineoplastic medicine; and when being combined with pharmaceutically acceptable supplementary material, the corosolic acid can be prepared into various oral, injection and external preparations and has better development and application prospect. On the basis of discovering that the corosolic acid has stronger antineoplastic action, the inventor further explains the mechanism of the antineoplastic action of the corosolic acid, thus retarding cancer cells at S period and inducing cell apoptosis mediation.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of corosolic acid in the preparation of antitumor drugs. Background technique [0002] At present, malignant tumors are the most serious diseases that endanger human health. According to statistics, about 50 million people die of cancer every year, and about 900,000 people die of cancer every year in China. Tumor is a new organism formed by abnormal proliferation of cells in local tissues under the action of various carcinogenic factors in the body, and often manifests as a local mass. Currently, tumor treatment methods include surgery, radiation, anticancer drugs, traditional Chinese medicine, and immunity. Among them, drug therapy is one of the most important means of clinical tumor treatment. At the same time, the research and development of anti-tumor drugs is an important direction and hot spot in the development of the global pharmaceutical industry, whic...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61P35/00
Inventor 凌昌全辛海量徐燕丰苏永华盛佳钰
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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