Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Modified method for chemically synthesizing N-acetyl-L-carnosine

A chemical synthesis and carnosine technology, applied in organic chemistry and other fields, can solve the problems of harmful eyes, racemization, and many steps of histidine methyl ester, and achieve the effect of simple process and guaranteed quality

Inactive Publication Date: 2007-11-28
NANJING RALLY BIOCHEM
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] As an industrial production process, the above-mentioned methods all have defects. The relatively simple process of the first method is its advantage, but it brings the problem of racemization; the second method involves too many steps of histidine methyl ester, and uses the right Condensing agent N, N-dicyclohexylcarbodiimide harmful to human eyes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Modified method for chemically synthesizing N-acetyl-L-carnosine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] L-carnosine 90.4g (0.40mol) is dissolved in 200ml 8% sodium hydroxide aqueous solution, under stirring, temperature of reaction must keep on 5-10 ℃, pH is in the scope of 13.0-13.5, drops 95% acetyl chloride 34.5g simultaneously ( 0.44mol) and 260ml of 50% sodium acetate aqueous solution. After completion of the dropwise addition, stir again at the same temperature for 1 hour, and check by HPLC to confirm that the product N-acetyl-L-carnosine is generated, and the disappearance of the raw material and the absence of the by-product N-acetyl-N-(imidazole) acetyl-carnosine . The reaction liquid was treated with strong acidic ion exchange resin SKIB to absorb N-acetyl carnosine, then washed with neutral water, concentrated the original eluate to 200ml with 1mol / L ammonia water, added acetic acid to adjust the pH to 4.9, and kept the temperature at 50 ℃, add 780ml of isopropanol, keep stirring at 50 ℃ for 3 hours, lower to 20 ℃ and stir for another 1 hour, the crude product...

Embodiment 2

[0019] 45.2 g (0.20 mol) of L-carnosine was dissolved in 55 ml of 8% aqueous sodium hydroxide solution, and the pH was adjusted to 13.3, while 20.4 g (0.26 mol) of 95% acetyl chloride and 180 ml of 50% sodium acetate solution were added dropwise. The drop was completed in 1.5 hours, during which the pH of the solution was 12.5-13.5, and the reaction temperature was maintained at 18-20°C. After the dropwise addition, stir at the same temperature for 1 hour, and simultaneously follow up with HPLC to confirm the generation of the reaction object, the raw material and the by-reaction product N-acetyl-N 2 -Acetyl-L was not detected.

[0020] Treat the reaction liquid with strong acid resin according to Example 1, adjust the pH and crystallize with isopropanol to obtain 42.4 g of N-acetyl-L-carnosine, the yield is 80.0%, and the specific rotation [α] 20 D =+26.10° (C=3, water), no racemization confirmed.

Embodiment 3

[0022] 67.8g (0.30mol) of L-carnosine was dissolved in 200ml of water, 200ml of acetone and 166ml of 8% sodium hydroxide solution to adjust the pH to 12.5. The solution was stirred, and the reaction temperature was kept at 10°C. At the same time, 25.9 g (0.33 mol) of 95% acetyl chloride, acetone solution and 50% sodium acetate aqueous solution were added dropwise. The pH of the reaction was maintained at 11.5-12.5 during the dropwise addition. After the dropwise addition, stir at room temperature for 30 minutes, evaporate acetone under reduced pressure, add 200 ml of water, and treat N-acetyl-L-carnosine as in Example 1 to obtain 76.5 g of crystals with a yield of 77.5% and a melting point of 216-218°C. Specific rotation [α] 20 D =+26.0° (C=3, water), no racemization confirmed.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to improved chemical synthesis process of N-acetyl-L-carnosine. The main material L-carnosine is acetylated with acetyl chloride as the acetylating agent through reaction in alkaline water solution at proper temperature and pH value in the presence of sodium acetate as acid binding agent. The process has N-acetyl-L-carnosine yield of 77.5-82.0 % and no racemisation.

Description

field of invention [0001] The invention relates to the field of organic medicinal chemistry, in particular to an improved synthesis method of N-acetyl-L-carnosine, a health care and treatment drug. Background technique [0002] N-acetyl-L-carnosine (N-Acyl-L-Carnosine), exactly N-acetyl-β-alanine-L-histidine, its chemical structure is: [0003] [0004] Because N-acetyl-L-carnosine has a strong control effect on the circulation around the brain, it can improve attention and memory functions; especially the aluminum salt of N-acetyl-L-carnosine can be used as a preventive and healing agent for digestive tract ulcers agent, and promote its application and promotion in nutritional health care and medical treatment. [0005] Generally speaking, chemical synthesis can be divided into two types in terms of structure: [0006] (1) A.Lukton and A.Sisti, J.Org.Chem.26, 617 (1961), proposed to react L-carnosine with acetic anhydride; [0007] (2) G.Bailin and A.Lukton, J.Org.Che...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D233/64
Inventor 陈新王凯文
Owner NANJING RALLY BIOCHEM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com