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Stem cell composition containing adipose derived stem cell exogenous protein extract, preparation method of extract and application thereof

A technology of protein extracts and stem cells, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve problems such as water content reduction, skin thinning, and elasticity decline, and achieve simple ingredients and broad application prospects Effect

Inactive Publication Date: 2019-04-16
嘉文丽(福建)化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This cross-linking reaction reduces the permeability of collagen and elastin, reduces elasticity, thins the skin, reduces water content, etc., which in turn causes the skin to lose its plump appearance and elasticity, resulting in sagging and wrinkles

Method used

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  • Stem cell composition containing adipose derived stem cell exogenous protein extract, preparation method of extract and application thereof
  • Stem cell composition containing adipose derived stem cell exogenous protein extract, preparation method of extract and application thereof
  • Stem cell composition containing adipose derived stem cell exogenous protein extract, preparation method of extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 A preparation method of a stem cell composition for skin anti-glycation

[0057] It is obtained by mixing the exocrine protein extract of adipose-derived mesenchymal stem cells, milk thistle extract, tea polyphenols, L-carnosine and evening primrose extract. The exocrine protein extract of adipose-derived mesenchymal stem cells is extracted from the culture supernatant of adipose-derived mesenchymal stem cells.

Embodiment 2

[0058] Example 2 Preparation method of exocrine protein extract from adipose-derived mesenchymal stem cells

[0059] This embodiment is the preparation method of the exocrine protein extract of adipose-derived mesenchymal stem cells, and the experimental steps are as follows: (1) according to the inoculation density 1×10 5 Adipose-derived mesenchymal stem cells were inoculated into T75 culture flasks and cultured in a 37°C, 5% carbon dioxide incubator;

[0060] (2) When the adipose-derived mesenchymal stem cells grow and proliferate to a density of 95%-100%, discard the supernatant medium, rinse with 5mL normal saline twice, then add 10mL normal saline, and place the cells at 7°C , 1.0%O 2 Hypoxic starvation induction culture was carried out in a three-gas incubator;

[0061] (3) After the adipose-derived mesenchymal stem cells were cultured for 36 hours after being induced by hypoxic starvation, the culture supernatant was collected;

[0062] (4) The collected culture supe...

Embodiment 3

[0065] Example 3 A preparation method of an anti-glycation lotion containing a stem cell composition

[0066] This example is a preparation method of an anti-glycation lotion containing a stem cell composition, and its formula is as follows in terms of weight percentage:

[0067]

[0068]

[0069] The manufacturing process of this embodiment is as follows:

[0070] (1) Dissolve phase B first and set aside;

[0071] (2) Then heat phase A to 50°C, stir and dissolve until uniform;

[0072] (3) When phase A is cooled to 40°C, add the premixed liquid of phase B, stir evenly and discharge.

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Abstract

The invention belongs to the technical field of beautifying, and mainly relates to a stem cell composition containing an adipose derived stem cell exogenous protein extract, a preparation method of the extract and application thereof. The composition includes the components of adipose derived stem cell exogenous protein extract, milk thistle extract, tea polyphenol, L-carnosine and evening primrose extract. The preparation method of the composition comprises the following steps: a) collecting an adipose derived stem cell culture supernatant and conducting ultra high speed centrifuging to collect a stem cell exogenous protein; b) mixing the milk thistle extract, the tea polyphenol, the L-carnosine, the evening primrose extract and the adipose derived stem cell exogenous protein extract. Thecomposition is simple in component, the stem cell protein used is derived from auto adipose derived stem cells, the material is not limited, no rejection reaction exists, and the anti-glycation effect has broad application prospects in anti-aging cosmetics.

Description

technical field [0001] The invention belongs to the technical field of cosmetology, and in particular relates to a stem cell composition containing an exocrine protein extract of adipose-derived mesenchymal stem cells, a preparation method of the extract, and an application of the composition. Background technique [0002] Skin aging is usually divided into endogenous aging and exogenous aging. Endogenous aging is also called natural aging. Related theories and hypotheses include free radical theory, genetic theory, mitochondrial theory, telomere theory, and immune decline theory, etc.; exogenous aging is also called photoaging, which is mainly caused by ultraviolet light. Damage, including the denaturation of nucleic acid or protein in skin epidermal cells, the reduction of hyaluronic acid content, the denaturation of collagen or elastin, etc. will cause the skin to relax and sag. As researchers continue to study and explore the mechanism of skin aging, at present, in addi...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/99A61K8/9794A61K8/9789A61K8/49A61Q19/08
CPCA61K8/498A61K8/64A61K8/99A61Q19/08A61K8/9789A61K8/9794
Inventor 饶焕文林建平萧自智潘发伍叶美娜王希丽王振华
Owner 嘉文丽(福建)化妆品有限公司
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