54 results about "Cell culture supernatant" patented technology

The invention discloses a sleep-promoting nasal spray based on mesenchymal stem cell-derived exosomes and preparation and application thereof.The preparation method comprises the steps that 1, mesenchymal stem cells separated from umbilical cord tissue are cultured to P3-P5 generations, cell culture supernate is collected, cells are digested, and then cell debris and macromolecular impure protein are removed through pretreatment; (2) filtering and concentrating the pretreated cell culture supernatant and replacing a part of solution; (3) carrying out degerming filtration on the solution obtained in the step (2) through a 0.22 [mu] m filter membrane to obtain an MSC-Exos solution; and (4) adding a stabilizer with a proper concentration into the MSC-Exos solution to obtain a compound MSC-Exos solution, namely the sleep-promoting nasal spray. The product is a cell-derived natural active substance, can significantly improve sleep, and does not involve any pharmaceutical ingredient.

The invention discloses a skin-tightening anti-wrinkle essence and a preparation process thereof, and relates to the technical field of cosmetic preparation, and the preparation process comprises the following steps: obtaining animal umbilical cord tissue, culturing, collecting cell culture supernatant after culture, carrying out low-speed centrifugation to remove cells and dead cells, and carrying out concentration, filtration and sterilization through a tangential flow membrane technology to obtain the skin-tightening anti-wrinkle essence. The animal umbilical cord extract is obtained; the preparation method comprises the following steps: mixing the animal umbilical cord extract with water, butanediol, glycerol, hydroxypropyl tetrahydropyrantriol, 1, 2-hexanediol, trehalose, p-hydroxyacetophenone, allantoin, hydrolyzed sodium hyaluronate, sodium hyaluronate, tetrahydromethylpyrimidine carboxylic acid, xanthan gum and essence, homogenizing under a stirring condition, and standing for defoaming. By constructing an active stable microenvironment and an efficient transdermal delivery system, the technical problem that the animal umbilical cord extract serving as a core component is easy to inactivate and difficult to absorb is solved, and a faster and more stable skin structure remodeling effect is realized by utilizing the synergistic effect of the animal umbilical cord extract and functional components.

The invention discloses a purification method of cord blood mesenchymal stem cell source exosome, and relates to the technical field of bioengineering. The method comprises the following steps: firstly, carrying out pH regulation pretreatment on cell culture supernate through a technical path of combining gradient centrifugation and membrane filtration, and sequentially removing cell debris and large-particle impurities by adopting multi-stage differential centrifugation; then carrying out selective protein precipitation by utilizing polyethylene glycol with specific molecular weight, and realizing fine separation of the exosome by combining sucrose-meglumine diatrizoate density gradient centrifugation; and finally, terminal purification is completed under low-temperature and low-pressure conditions through a polyethersulfone membrane filtration system with an optimized pore size. According to the method, centrifugal parameters, reagent concentration and filtering conditions are precisely regulated and controlled, so that the particle size of the obtained exosome is intensively distributed in the range of 30-150nm, the protein purity reaches 95% or above, the recovery rate exceeds 85%, and the separation efficiency and quality of the exosome are remarkably improved.

The invention discloses an efficient purification process of a bispecific antibody and a special chromatography system thereof. The efficient purification process of the bispecific antibody comprises the following steps: pretreatment: performing 0.22 mu m microfiltration on a cell culture supernatant to remove cell debris, and performing deep filtration to reduce turbidity to be less than or equal to 1NTU so as to reduce subsequent chromatography medium pollution; carrying out improved Protein A affinity chromatography, namely carrying out affinity chromatography by adopting an agarose medium modified by a recombinant Protein A ligand, taking 20mM Tris-HCl as a loading buffer solution, and taking the pH value of the loading buffer solution as 7.4, taking 100mM citric acid as an elution buffer solution, and taking the pH value of the elution buffer solution as 3.2-3.5; performing mixed mode chromatography: adopting a composite mode medium containing a benzamidine functional group, taking 50mM sodium phosphate as a buffer solution, adjusting the pH value to 6.0-6.5, adjusting the NaCl concentration to 0.1-0.3 M, and specifically removing a double-antibody polymer and a mismatched isomer by utilizing a hydrophobic effect and an ion exchange synergistic effect; and performing nanoscale filtration and terminal treatment: removing viruses by adopting a virus filtration membrane with the aperture of 30nm, and performing 0.22 mu m sterile filtration to obtain a final product. According to the process, the purification efficiency, the yield and the product purity are remarkably improved, and the process is suitable for efficient purification of the bispecific antibody.

The present application relates to a kind of water gel support prepared by self-assembled polypeptide and the use of support obtained therefrom, the preparation method includes: 1) self-assembled peptide sequence is linked nerve growth factor analog peptide by covalent bond to obtain bonded functional polypeptide;2) macrophage is separated and cultured, and is induced to be " alternative activation " anti-inflammatory M2 macrophage, obtain culture supernatant and carry out filtration, obtain filtered cell culture supernatant;3) filtered cell culture supernatant is mixed with bonded functional polypeptide, obtain mixed solution and adjust the concentration of the mixed solution, the bonded functional polypeptide self-assembles and forms water gel support.The present application uses M2 macrophage condition culture supernatant to construct regenerative microenvironment, combines polypeptide functional water gel support, realizes better overall interaction, and prepares the water gel support that can supplement and regulate nerve regeneration, the support can promote nerve cell regeneration behavior, provides new selection for tissue engineering biomaterials.

The invention relates to the technical field of bioengineering, in particular to a method for extracting exosomes from microspore ganoderma lucidum immune protein.The method comprises the steps that microspore ganoderma lucidum immune protein extraction comprises pretreatment, extraction, centrifugation, salting-out, dialysis and chromatographic purification; exosome is extracted from cell culture supernate by using the immune protein through an immunoaffinity capture method, and the method comprises the steps of incubation combination, centrifugal separation and elution purification. The microspore ganoderma lucidum immune protein is specifically combined with the exosome for the first time, the extraction method is efficient, mild and low in cost, and the extracted exosome is high in purity and good in integrity, has double activities of the exosome and the ganoderma lucidum immune protein, and has wide application prospects in the fields of disease diagnosis, drug delivery, immune regulation and the like.

This invention provides the application of apatinib in the preparation of antiviral drugs, specifically involving the application of the anticancer drug apatinib in inhibiting the replication of Hantanensis virus (HTNV) and Chikungunya virus (CHIKV). Apatinib can significantly inhibit HTNV replication and reduce viral titers at the cellular level. Experiments have shown that after treatment with apatinib, the expression levels of viral structural proteins, the nucleic acid levels of HTNV, and the viral titers in the cell culture supernatant were significantly reduced in HTNV-infected A549 cells, while the drug exhibited low cytotoxicity. Furthermore, apatinib can also inhibit CHIKV replication. This invention provides a novel anti-HTNV candidate drug and offers insights for the rapid development of antiviral drugs.

This invention discloses a method for preparing cell-derived exosome subsets, as well as products and applications, belonging to the field of biomedical technology. The preparation method includes: filtering cell culture medium to obtain a cell culture supernatant; concentrating the supernatant 5-30 times using a tangential flow system with a 300-500 kDa hollow fiber filter, followed by 10-20 times washing to obtain a concentrated solution; and performing Sephacryl S-400 HR gel filtration column chromatography, collecting the eluent from two 280 nm UV absorption peaks to obtain exosome subsets S1 and S2; the protein concentration of the concentrated solution is 20-50 mg / mL. This invention achieves, for the first time, precise separation of functional exosome subsets. Subset S1 exhibits strong immunomodulatory activity, and subset S2 demonstrates excellent cell regeneration promotion effects. Furthermore, the process is highly versatile and suitable for large-scale production. These subsets can be used to prepare immunomodulatory or cell regeneration-promoting drugs, providing precise technical solutions for the treatment of related diseases.

The application discloses a kind of based on HIV envelope protein chimeric exosome and its preparation method and application, belong to biological medicine technical field.The application first constructs the cell line of stable expression HIV envelope protein Env, obtains engineered exosome from cell culture supernatant separation and purification;The exosome is used as immunogen combined with adjuvant immunization experimental animal, and high-efficiency induction specific humoral immune response;Again, obtain Env antigen specificity single B cell by flow cytometry sorting, obtain antibody variable region gene by single cell lysis, reverse transcription and nest PCR amplification, cloning to expression vector and expressing in mammalian cell, finally, HIV specific neutralizing antibody is screened by binding activity, affinity and neutralizing activity;The application is combined with single B cell antibody screening technology by embedding HIV Env antigen in the form of membrane combination in exosome surface, and realizes the synergistic optimization of antigen delivery and antibody screening process.

The invention discloses a method for establishing a rat bionic model for sudden deafness caused by inner ear microcirculation disturbance, and relates to the technical field of animal model construction.The method comprises the steps that exosome extraction and purification are conducted, specifically, healthy rat bone marrow is obtained, mesenchymal stem cells are extracted and placed in a culture bottle to be cultured, cell culture supernate is collected, and the exosome is obtained; extracting exosomes by adopting an ultracentrifugation method; loading of the procoagulant factor and the exosome: selecting a prothrombin fragment as the procoagulant factor, treating the prothrombin fragment, and mixing the obtained exosome resuspension and the prothrombin fragment solution according to the volume ratio of 1: 1. The exosome derived from the mesenchymal stem cells is adopted as a procoagulant factor carrier, the source is wide, and the exosome is easy to obtain; the exosome is stable in yield and good in biocompatibility, damage to inner ear tissues of rats can be reduced, and the exosome has natural targeting transportation capacity and can be specifically combined with inner ear vascular endothelial cells, so that the concentration of a procoagulant factor at a target site is increased, and the accuracy of model establishment is enhanced.

The present invention relates to a method of purifying whole HCV particles, the method comprising the steps of a) providing a cell culture supernatant comprising virus particles, b) purification and / or concentration of the cell culture supernatant, c) purification and / or concentration of the product of above step b) using steric exclusion chromatography (SXC) at alkaline pH in the range of 8-10, d) purification and / or concentration of the product of the above step c) using sulphated cellulose membrane absorbers (SCMA), e) obtaining whole virus particles.

The invention relates to the technical field of biological medicine and polymer targeted delivery, and provides an oligodendrocyte-derived exosome and application thereof in preparation of a medicine for treating traumatic brain injury. Comprising the following steps: separating and culturing primary oligodendroglia cells from brain tissues; placing the maturely cultured oligodendroglia cells in a sugar-free and serum-free culture medium, performing oxygen-glucose deprivation treatment in an anoxic environment, then continuously culturing in a normal sugar-containing culture medium under a normal oxygen condition, and collecting cell culture supernate; and separating the exosome of the oligodendroglia cells subjected to oxygen-glucose deprivation treatment from the supernate by adopting a gradient ultracentrifugation method to obtain the exosome of the oligodendroglia cells subjected to oxygen-glucose deprivation treatment. The exosome derived from the oligodendroglia is applied to preparation of the medicine for treating the traumatic brain injury and has the advantages of being high in targeting performance and good in penetrability, that is, the exosome is used as a natural carrier, the defect that a traditional traumatic brain injury medicine is difficult to penetrate through a blood brain barrier is perfectly overcome, and the exosome can efficiently enter brain tissue and be taken by microglia.

The application discloses a transient transfection method for improving recombinant protein expression of CHO cells, and relates to the technical fields of eukaryotic cell gene transfection and animal cell culture. A cell suspension is obtained by mixing a CHO cell and a serum-free CHO cell basic culture medium; DNA and a transfection reagent are added into the cell suspension to mix a transfection culture, 5-azadeoxycytidine acid solution is added into the transfection culture at 0h-24h to form a transfection system and continue the transfection culture; the transfection system is added with a feeding culture medium at 1d-4d after the transfection culture to carry out suspension culture; the suspension culture is centrifuged at 7d to separate and harvest cell culture supernatant to complete recombinant protein expression. According to the application, 5-azadeoxycytidine acid is added into the transfection culture at a specific concentration, so that the expression of recombinant protein of the CHO cell is significantly improved during the transfection expression.

The application relates to a method for purifying umbilical cord blood mesenchymal stem cell-derived exosomes, and relates to the technical field of bioengineering. The method combines gradient centrifugation and membrane filtration. First, the cell culture supernatant is pretreated by adjusting the pH, and cell debris and large-particle impurities are removed by multi-stage differential centrifugation. Then, selective protein precipitation is carried out by using polyethylene glycol with a specific molecular weight, and the exosomes are finely separated by sucrose-meglumine diatrizoate density gradient centrifugation. Finally, terminal purification is completed under low-temperature and low-pressure conditions by using a polyether sulfone membrane filtration system with optimized pore size. By precisely controlling the centrifugation parameters, reagent concentration and filtration conditions, the exosomes obtained by the method have a particle size concentrated in the range of 30-150 nm, a protein purity of more than 95%, and a recovery rate of more than 85%, and the separation efficiency and quality of the exosomes are significantly improved.

The invention discloses a microplastic degrading enzyme as well as a preparation method and application of the microplastic degrading enzyme. The preparation method comprises the following steps: cloning a fast-petase gene into a pFastBacTM Dual vector, so as to obtain a pFastDual-fast-petase vector; the method comprises the following steps: converting a pFastDual-fast-petase vector into competent cells, coating the competent cells on a flat plate for blue-white shift culture, selecting a white bacterial colony, and extracting recombinant bacmid-fast-petase DNA (Deoxyribose Nucleic Acid); the method comprises the following steps: transfecting recombinant bacmid-fast-petase DNA (deoxyribonucleic acid) into silkworm culture cells, culturing until the cells have diseases, and collecting cell culture supernatant to obtain a recombinant virus rBmNPV-fast-petase; bombyx mori culture cells or bombyx mori are infected with the recombinant virus rBmNPV-fast-petase, after the bombyx mori is attacked, supernate of the bombyx mori culture cells or bombyx mori tissue is collected and then purified, and the microplastic degrading enzyme is obtained. According to the invention, high-yield expression and high-purity preparation of the PET enzyme are realized quickly, and an efficient and sustainable solution is provided for biodegradation of micro-plastic pollution. The success of the invention not only helps to alleviate the global microplastic pollution problem, but also provides important reference for the biodegradation technology of other plastic wastes.

The present disclosure relates to methods for characterizing small amounts of immunogen-binding proteins, such as immunogen-binding antibodies, obtained directly from the supernatant of individually cultured B cells. These methods involve providing a preparation of B cells that avoid hybridoma formation and secrete immunogen-binding antibodies, and individually culturing the B cells under conditions suitable for the B cells to secrete the immunogen-binding antibodies into the culture supernatant. The culture supernatant containing the secreted antibodies is collected from the individually cultured B cells and subjected to one or more binding assays to characterize the immunogen-binding antibodies from the B cell preparation (e.g., for their binding affinity by exposing the cell culture supernatant to increasing concentrations of immunogen).

The invention discloses a preparation method, a product and application of a cell-derived exosome subset, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: filtering a cell culture solution to obtain a cell culture supernatant; concentrating by 5-30 times by using a tangential flow system of a 300-500kDa hollow fiber filter, and washing and filtering by 10-20 times to obtain a concentrated solution; carrying out Sephacryl S-400 HR gel filtration column chromatography, and collecting two pieces of ultraviolet absorption peak eluent with the wavelength of 280 nm, so as to obtain exosome subgroups S1 and S2; the sample protein concentration of the concentrated solution is 20 to 50 mg / mL. According to the method, accurate separation of exosome functional subgroups is achieved for the first time, the S1 subgroup is high in immunomodulatory activity, the S2 subgroup is excellent in cell regeneration promoting effect, the technology universality is high, and large-scale production can be achieved. The subgroup can be used for preparing medicines for regulating immunity or promoting cell regeneration, and a precise technical scheme is provided for treating related diseases.

The present invention relates to the fields of medicine, cell biology, molecular biology and genetics. In particular, the present invention provides methods to isolate and purify microvesicles from cell culture supernatants and biological fluids. The present invention also provides pharmaceutical compositions of microvesicles to promote or enhance wound healing, stimulate tissue regeneration, remodel scarred tissue, modulate immune reactions, alter neoplastic cell growth and / or mobility, or alter normal cell growth and / or mobility. The present invention also provides compositions of microvesicles to be used as diagnostic reagents, and methods to prepare the compositions of microvesicles.

The invention discloses an extraction method and application of membranous small vesicles. The extraction method comprises the following steps: collecting a biological sample, and selecting at least one of blood, urine and cell culture supernatant; centrifuging the sample at 10,000 xg for 20 minutes, and removing cell debris and apoptotic bodies to obtain a pretreated sample; adding 20% by volume of sample separation liquid and CD63 / CD81 antibody modified magnetic beads into the pretreated sample, and incubating and capturing target vesicles; freezing the captured vesicles with liquid nitrogen for 15-35 minutes, and dissolving the vesicles with a 33-43 DEG C water bath; and cracking the dissolved vesicles with 0.5 W / cm < 2 > ultrasonic waves to finally obtain the intact vesicles of 30-70 nm and soluble protein factors. Original plasma or urine can be directly treated, dilution is not needed, and loss of a target object is avoided; a low-cost and degradable biomimetic polymer is developed to replace an imported kit, and the cost is reduced by 75%; the whole process lt; the time is 2.5 hours, which is superior to that of an ultracentrifugation method; and the extracted membranous small vesicles have good integrity and are suitable for liquid biopsy and drug carrier construction.

The present invention relates to a method for rescue of Vesicular Stomatitis Virus (VSV) from DNA in a HEK293 cell line or a HEK293 cell line adapted to suspension growth comprising (a) providing cells from a HEK293 cell line or a HEK293 cell line adapted to suspension growth in cell culture, (b) transfecting the cells with at least one plasmid, wherein the at least one plasmid comprises (i) an expression cassette comprising a VSV genomic cDNA; (ii) at least one expression cassette encoding VSV nucleoprotein (N) protein, VSV phosphoprotein (P) protein, and VSV large (L) protein; and (iii) an expression cassette encoding SV40 Large T antigen; (c) culturing the transfected cells; and (d) harvesting the cell culture supernatant comprising the rescued VSV. Also provided is the use of a HEK293 cell line or a HEK293 cell line adapted to suspension growth for rescue of Vesicular Stomatitis Virus (VSV) or the use of a plasmid encoding SV40 Large T antigen for rescue of Vesicular Stomatitis Virus (VSV) in a HEK293 cell line or a HEK293 cell line adapted to suspension growth HEK293-F cells by means of transient transfection.

The invention discloses a recombinant monoclonal antibody and application thereof in mouse antibody pair screening, the recombinant monoclonal antibody comprises a heavy chain and a light chain, a heavy chain variable region of the heavy chain comprises three complementary determining regions with the following amino acid sequences: CDRH1: SEQ ID NO.1, CDRH2: SEQ ID NO.2, and CDRH3: SEQ ID NO.3; a light chain variable region of the light chain comprises three complementary determining regions with the following amino acid sequences: CDRL1: SEQ ID NO.4, CDRL2: SAS, and CDRL3: SEQ ID NO.5. The invention also discloses application of the recombinant monoclonal antibody in screening of mouse antibody pairs. When the recombinant monoclonal antibody is used for screening mouse antibody pairs, antibody purification and marking can be avoided, serum-containing cell culture supernatant is directly used for detection, and pairing reactivity screening of a large number of antibodies is completed in a time-saving, labor-saving, efficient and low-cost manner.

The present invention relates to the field of producing protein conjugates. The invention relates to a novel method for producing a protein conjugate comprising a protein of interest (POI) bearing a chemical moiety and a payload comprising a complementary chemical moiety. The said method comprises the extracellular expression of the POI, contacting the POI in the cell culture supernatant with the payload, allowing the formation of a covalent bond between the POI and the payload in a cell culture supernatant, thereby creating a protein conjugate, and purifying said conjugate.

The invention relates to the technical field of cell biology and metabolism research, in particular to a standardized quantitative fat cell lipolysis method, which comprises the following steps: separating and purifying rat epididymis primary fat cells, inoculating to a porous plate, treating by using a reagent, and quantifying fat cell lipolysis by using the specific gravity difference between the fat cells and an aqueous solution. The glycerin release analysis of the cell culture supernatant and the MTT cell activity analysis of the fat cells are sequentially completed in the same hole; and finally, calculating the ratio of the glycerin release light absorption value to the cell activity light absorption value to realize the standardized quantification of the lipolysis level. According to the method, cell activity is introduced as an internal reference, cytotoxicity and operation error interference are effectively eliminated, particularly, the consumption of cell samples is reduced through a detection mode completed in the same hole, limited primary adipocytes obtained from a single experimental animal can support more experimental groups or repetition times, and the detection accuracy is improved. The experimental efficiency and the data output are improved, and the method is suitable for high-throughput screening and evaluation of obesity and metabolic syndrome related drugs.

The application relates to the technical field of exosome extraction, and discloses an exosome enrichment chip and an enrichment method.The chip comprises a sample storage box, a sample inlet channel, a vortex reaction enrichment pool, a sample circulation channel and the like.The enrichment method can be used for single-time and high-throughput circulation enrichment of exosomes;exosome-specific enrichment materials are loaded on the filter membrane at the bottom of the vortex reaction enrichment pool, and the asymmetric impinging streams of the bilateral asymmetric sample inlet channels can be matched with the vortex motion, so that the residence time of the exosomes in the enrichment pool and the collision efficiency of the exosomes with the specific enrichment materials are improved, efficient enrichment is realized, then the sample passes through the filter membrane and enters the sample circulation channel, and is returned to the sample storage box, circulation enrichment of the exosomes in the sample is realized, and the exosome recovery rate is improved.The application can realize rapid, high-yield and high-purity enrichment of exosomes in large-volume samples such as urine, thoracic and abdominal fluid and cell culture supernatant.

The invention discloses a preparation method and application of a vesicle vaccine based on a chitosan antigen delivery system, the vesicle vaccine stimulates cells to secrete the vesicle vaccine with an antigen presentation function, and the method related to the system specifically comprises the following steps: step 1, mixing antigen protein with a chitosan aqueous solution and a sodium tripolyphosphate aqueous solution, and carrying out electrostatic interaction to obtain a vesicle vaccine solution; chitosan nanoparticles loaded with the antigen protein are prepared; and 2, treating immune cells by using the antigen-loaded chitosan nanoparticles, collecting cell culture supernatant, and collecting and preparing vesicles by adopting a method of combining multi-step differential centrifugation with ultrafiltration. After the antigen delivery system based on chitosan is co-incubated with cells, antigen protein can be effectively delivered into immune cells, meanwhile, the immune cells are promoted to generate a large number of antigen-related vesicles with efficient immunogenicity through the adjuvant effect and autophagy regulation effect of chitosan, and the immunogenicity of the antigen-related vesicles is improved. The application potential of the artificial antigen presenting cell is realized.

The polypeptide OR-D-3 capable of improving the sexual function is obtained from oysters through enzymolysis screening and identification, and the polypeptide can improve the cell survival rate, increase the secretion amount of TM3 cell testosterone and increase the NO concentration of TM3 cell culture supernatant. After the polypeptide is applied to a mouse, the riding latency period and the riding frequency can be remarkably prolonged, the content of testosterone in serum is increased, no obvious toxicity exists, the sexual capacity of the mouse is remarkably improved, and a remarkable effect is achieved. The polypeptide has a good effect after being prepared into a medicine, and the application prospect is wide.

Herein is reported a method for purifying cell cultivation supernatants either directly after fermentation or after one or more preliminary purification steps, such as protein A affinity chromatography. By adjusting the pH value in the acid range and subsequent incubation of the acidified solution host cell nucleic acid and host cell protein can be precipitated but the target polypeptide remains in solution. Thereafter the precipitate and therewith the contaminating host cell components can be removed by a simple physical separation step.

The application obtains a polypeptide OR-D-3 with improved sexual function from oysters through enzymatic hydrolysis screening and identification, the polypeptide can improve cell survival rate, also can improve the secretion amount of TM3 cell testosterone, increase the NO concentration of TM3 cell culture supernatant. After the polypeptide is used for mice, the mount latency and mount frequency can be significantly prolonged, the content of testosterone in serum is improved, and there is no obvious toxicity, the sexual ability of mice is significantly improved, and the effect is significant. The polypeptide prepared into a drug has good effect, and has wide application prospect.

The invention relates to the technical field of biological medicine, in particular to a gene recombination cell exosome and application thereof in preparation of anti-cancer drugs. The gene recombinant cell exosome is extracted from an IL15K562 cell culture supernatant by the following method, and the method comprises the following steps: (1) collecting the IL15K562 cell culture supernatant, and centrifuging to remove cell debris; (2) adding a treatment reagent, uniformly mixing, and incubating for 6-20 hours; and (3) centrifuging to obtain a precipitate, further suspending the precipitate in a buffer solution, and centrifuging again to obtain the gene recombinant cell exosome. Researches show that the gene recombinant cell exosome prepared from the IL15K562 cell culture supernatant by the method disclosed by the invention has a remarkable anti-cancer effect; therefore, the compound has important application value when being used as an active ingredient for preparing the anti-cancer drugs.