114results about How to "Reduce the cost of separation and purification" patented technology

The invention belongs to the technical field of extraction and purification of plant ingredients and particularly relates to a method for extracting and purifying cannabidiol from China-hemp. In the method, an ingredient of cannabidiol is extracted from floral leaves in a mature period of the China-hemp according to a supercritical CO2 extraction technique, a cannabidiol extract is further purified by macroporous adsorbent resin and a rapid purification system, a cannabidiol content of an obtained product is 98% or higher, and the cannabidiol product does not contain a psychotoxic ingredient tetrahydrocannabinol. According to the method, a chemical organic solvent is not used in an extraction process, so that the method is environment-friendly and is low in toxicity and high in efficiency; the loss of cannabidiol in an adsorption elution process is reduced in a purification process, so that the impurity ingredients such as tetrahydrocannabinol and the like are effectively removed, and the use of an organic regent is decreased, so that the purification cost is lowered, and the environmental pollution is reduced. The product is high in yield and purity, and the requirement for development of a value-added product of the China-hemp, in particular to the market requirement of the active ingredient of the cannabidiol product in the China-hemp, can be met, and especially, the cannabidiol product has the great application advantage in the pharmaceutical field.

The invention relates to a method for continuously preparing 1, 3-propylene glycol by glycerin through one step of conversion. With the presence of metal / solid acid bifunctional catalyst, glycerin water solution and hydrogen are simultaneously and continuously led into a fixed bed reactor and have a catalytic reaction at the temperature of 100-150 DEG C, and a pressure of 2-8MPa, wherein, the volume ratio of hydrogen and glycerin water solution is 600-1200; volume air speed of glycerin water solution is 0.15-1h<-1>; the reaction product is collected and has a gas liquid separation to remove gas hydrogen; the liquid phase is distilled and separated to remove the side product to obtain the 1, 3-propylene glycol product; the metal / solid acid bifunctional catalyst is functioned by zirconia / tungsten oxide complex oxide with attachment of platinum; and weight ratio of each metallic element in the catalyst is: Pt: W: Zr=1-5:5-20:69-55. The process of the reaction is simple; the production can be continuous; high concentration glycerin can be converted under a relatively moderate reaction condition; content of 1, 3-propylene glycol is high, while content of side product is low; and 1, 3-propylene glycol separation and purification cost is low.

The invention discloses an Escherichia coli engineered strain and application in succinic acid production through the aerobic-microaerobic-anaerobic full-stage fermentation of the Escherichia coli engineered strain. The name of the engineered strain is Escherichia coli YL106. The genotype of the strain is a formula shown in a drawing. The strain is collected by China Center for Type Culture Collection (CCTCC) on April 17th, 2013 and has the collection number of CCTCC NO: M2013149. According to the engineered strain disclosed by the invention, succinic acid is produced under the condition of aerobic-microaerobic-anaerobic full-stage fermentation, a large amount of succinic acid can be accumulated efficiently, and the total productivity (2.13 g / (L.h)) of the engineered strain is the highest among literature reports related to the succinic acid production using Escherichia coli currently, so that the engineered strain disclosed by the invention and an aerobic-microaerobic-anaerobic full-stage succinic acid fermentation process have considerable industrial application values and prospects.

The invention relates to an Aspergillus niger gene engineering strain efficiently producing malic acid. The Aspergillus niger gene engineering strain is an Aspergillus niger gene engineering strain which knocks out a citric acid transporter gene cexA, and overexpresses a glucose transporter gene mstC, a hexokinase gene hxkA, a phosphofructokinase gene pfkA, and a pyruvate kinase gene pkiA which are derived from Aspergillus niger. The efficiency for producing malic acid of the Aspergillus niger gene engineering strain is significantly improved, the output on the eighth day through fed-batch fermentation in a fermentation tank reaches 195.72-210 g / L, a conversion rate of malic acid to glucose reaches 1.59-1.64 mol / mol, and a fermentation cycle is shorter by one day compared with a starting strain, namely the fermentation cycle is shortened from original 9 days to 8 days. An excellent strain for the preparation of malic acid by microbial fermentation methods is provided.

The invention discloses recombinant pichia pastoris for heterogenous high level secretory expression of a rhizomucor miehei lipase. The recombinant pichia pastoris is obtained by introducing a self leader peptide gene for encoding the rhizomucor miehei lipase by employing a molecular biology technology, then optimizing a copy number of an objective gene, and constructing a vector which can be expressed in pasteur pichia pastoris. Researches show that: the introduction of the self leader peptide greatly increases the secretion amount of the objective lipase, a pichia pastoris recombinant of a lipase gene (pro-rml) containing two copies has highest enzyme activity, and the lipase Pro-RML has higher activity of hydrolysis of tri-acylglycerol.

The invention relates to a method for extracting and separating cannabidiol. The method comprises the following steps of 1) pulverization, wherein dried industrial cannabis flowers and leaves are pulverized into granules by using a grinding disperser; 2) extraction, wherein ethyl alcohol, petroleum ether or solvent naphtha is taken as a single solvent or a mixed solvent to perform reflux extraction on the materials; 3) enrichment, wherein an industrial hemp fluid extract obtained by extraction and concentration is subjected to macroporous resin chromatography, and an eluate is collected in stages to obtain a cannabidiol enrichment; 4) separation, wherein the cannabidiol enrichment is subjected to silica gel column chromatography, and an eluate is collected in stages, concentrated, dried and crystallized to obtain the cannabidiol. The product has the advantages that the purity is higher, the extraction is convenient and fast, and the purification cost is lower.

The invention discloses a method of extracting squalene from bottom sediment with natural vitamin E extracted. The bottom sediment with the natural vitamin E extracted is used as the raw material to prepare high-content squalene through a saponification, rectification and silica gel column chromatography combined process. The method specifically comprises the steps that firstly, the bottom sediment is subjected to a saponification reaction, unsaponifiable matter obtained through extraction is subjected to separation in a secondary rectifying tower, distillate obtained through secondary rectification is used as the raw material and subjected to silica gel column chromatography separation, three organic solvents with different polarities are used for elution, and fractional collection is conducted to obtain a plant hydrocarbon component, a squalene component, a natural vitamin E component and a sterol component, wherein the squalene content of the squalene component is more than 95%. According to the method, the bottom sediment with the natural vitamin E extracted is used as the raw material for extraction of the squalene, the process is simple and convenient, separation efficiency is high, industrial production is easy, the obtained squalene content is high, crude natural vitamin E and sterol can also be obtained, and comprehensive utilization of effective ingredients in the bottom sediment is achieved.

The invention discloses a preparation method of arginine glucoside, which comprises the following steps: adding arginine and maltose into glacial acetic acid, adding a maillard reaction inhibitor, reacting for 10 min to 2 hours to obtain a total reactant; concentrating the total reactant under a low-temperature and vacuum condition to obtain an extract, stirring the extract with silica gel by a dry process, evaporating acetic acid, applying the extract to a 200-300-mesh silica gel column, eluting the column with 50-90% ethanol, collecting each flowing component, detecting the components by thin-layer chromatography, collecting a portion of the eluate with a Rf value of 0.2, concentrating the eluate under a low pressure, freeze-drying the eluate into powder, dissolving the powder in an aqueous acetic acid solution, applying the solution to a polyacrylamide column, eluting the column with the aqueous acetic acid solution, collecting each flowing component, detecting the components by thin-layer chromatography, collecting an eluate with a Rf value of 0.2, concentrating the eluate under a reduced pressure, and freeze-drying to obtain a product. The preparation method of the invention can increase the AFG conversion rate in the synthesis, reduce the generation of reaction by-products, decrease the generation of melanoid which is an end product of the maillard reaction, and thus greatly reduce the cost of the separation and the purification of AFG; the invention provides an medical effect of AFG on the prevention and the treatment of type 1 diabetes and type 2 diabetes.

The present invention provides a Pichia pastoris for highly efficient heterologous expression of white Penicillium cyclopium lipase and an enzyme production medium. According to the present invention, the white Penicillium cyclopium lipase containing a self leader peptide (7 amino acids) is heterologously expressed in a genetically engineered Pichia pastoris, increasing the activity of exogenous protein lipase, and improving a part of enzymatic properties of the exogenous protein lipase. According to the enzyme production medium of the invention, through optimizing the YNB component of a BMMY medium, the medium cost is reduced by 70%. According to the invention, reliable experimental basis is provided for the industrial application of the enzyme, especially for the catalyzed hydrolysis of substrate astaxanthin ester from Haematococcus pluvialis to produce astaxanthin monomers.

The invention provides a purification technology with a membrane separation strengthening function for hydrogen in high-hydrogen-content refinery gas through pressure swing adsorption, and belongs tothe field of petrochemical industry. According to the technology, an organic vapor membrane separation unit is added to a pre-treatment system of a pressure swing adsorption device, heavy hydrocarboncomponents of carbon 5 or above are efficiently and deeply removed, and the content of the heavy hydrocarbon components is reduced to 0.3 vol% or below; the regeneration conditions of an adsorption agent are improved, the service life of the adsorption agent is prolonged, and the long-term operation stability of the pressure swing adsorption device is improved; a hydrogen membrane separation unitis added to an after-treatment system, organic vapor membrane permeation gas is further recovered, and low-concentration hydrogen in tail gas is subjected to pressure swing adsorption, so that the content of hydrogen in fuel gas is lower than 15 vol%, and the total hydrogen yield exceeds 95%. By prolonging the service life of the adsorption agent and increasing the hydrogen recovery rate, by meansof the purification technology with the membrane separation strengthening function for the hydrogen in the high-hydrogen-content refinery gas through pressure swing adsorption, compared with a traditional adsorption technology, the hydrogen separation and purification cost of the technology can be reduced by 10-20%.

The invention discloses a method for extracting polyphenol from passion fruit peel. The method comprises the following steps: freeze-drying and crushing the passion fruit peel; carrying out ultrasonic wave-ethanol assisted extraction in a solution 30-80% ethanol under an ultrasonic power of 200-400 W at a temperature of 30-70 DEG C for 5-35 min, filtering the obtained solution by a 0.45-0.80 [mu]m nylon filter, and concentrating the filtered solution; and separating and purifying the obtained concentrate by macro-porous resin with the particle size of 0.30-1.25 mm, and carrying out vacuum freeze drying to obtain powder of polyphenol in the passion fruit peel. The extraction method ensures the extraction rate and the purity of the polyphenol through combining ultrasonic wave-ethanol assisted extraction, macro-porous resin separation and purification and vacuum freeze drying technologies. The extraction rate of the polyphenol in the passion fruit peel can reach 33.95-50.35%. The extraction method is a new method for effectively utilizing the passion fruit peel, and can change wastes into valuables.

The invention relates to the field of genetic engineering, microbiology, enzyme engineering and fermentation engineering, and provides a method for expressing and purifying a low temperature chitinasegene chiA (chitinase A) in kluyveromyceslactis.The method for expressing and purifying the low temperature chitinase gene chiA (chitinase A) in the kluyveromyceslactissuccessfully constructs a recombinant kluyveromyceslactis producing the low temperature chitinasechiA and purifiesthe recombinant kluyveromyceslactisto obtain a high purity recombinant chitinasechiA, and a pure recombinant chitinasechiA is obtained by using a nickel column affinity chromatography. After SDS-PAGE analysis, protein bands of the desired size appear near 110 kDa.According to the method for expressing and purifying the low temperature chitinase gene chiA (chitinase A) in the kluyveromyceslactis, the chitinasechiAexpressed is mostly secreted outside cell, thereby reducing the separation and purification cost and improving the expression efficiency.The protein concentration of purified protein chiAis 1.26 mg / mL and the enzyme activity is 51.45 U / mg.The purified chitinaseChiA has been studied in enzymatic properties such as temperature and temperature stability, pH and pH stability and synergistic degradation, and a foundation for industrial application of such enzyme is laid.

The invention discloses an antibody type biological magnetic microsphere as well as a preparation method and application thereof. On one hand, the invention provides the biological magnetic microsphere, at least one polymer with a linear main chain and branched chains is fixed onto the outer surface of a magnetic microsphere body, and tail ends of the branched chains of the polymer of the biological magnetic microsphere are connected with antibody type labels. The invention also provides a preparation method and application of the biological magnetic microsphere provided by the invention. The biological magnetic microsphere provided by the invention is convenient in operation and use and can be rapidly dispersed and rapidly settled in a solution, and large-scale experimental facilities such as a high-speed centrifugal machine are not required to be used; the biological magnetic microsphere is an antibody magnetic bead, and the antibody type labels can also be connected to the tail ends of the branched chains of the polymer through interaction of an affinity compound; and the use is wide, and the antibody type labels as a purification medium have flexible selectivity, and are universally and massively applied to separation and purification of a target object.

The invention relates to a method for synthesizing a flavone alkyl phosphate compound and application thereof in a cholesterol esterase inhibitor, which overcomes the disadvantages of the traditional method that CCl4 is used as a solvent for synthesizing organic phosphate, while flavone usually has a low solubility in CCl4 and thus is difficult to carry out a phosphorylation reaction in CCl4. In the method, DMSO (Dimethylsulfoxide), DMF (Dimethyl Formamide), acetonitrile, diethyl ether, dioxane, THF (Tetrahydrofuran), and the like, which have stronger polarity are used as solvents, and flavone has high solubility in the solvents; nitrogenous bases, such as triethylamine, diethylamine, pyridine, and the like, are used as alkali reagents; ClPO (OEt)2 (Diethyl Chlorophosphate) is used as a phosphorus esterification reagent; and DMAP (Dimethylaminopyridine) is used as a catalyst. Thus, the method has the advantages of high compounding reaction speed, mild reaction conditions, high chemical selectivity, high reaction efficiency and higher yield of a flavone phosphate compound with a novel structure. The new compound has the advantages of simple separation and purification method, high currency and low production cost, and the conditions required in the whole process from synthesis to separation and purification are suitable for condition requirements on production in a large scale. The new synthesized compound can be applied to preparing the cholesterol esterase inhibitor with a high activity, and the active IC50 of the cholesterol esterase inhibitor is within 3.76-390nM and shows a very obvious structure-activity relationship.

The invention discloses pegylation benzoindoles heptamethine cyanine dye and a preparation method and application thereof. A molecular structure of the pegylation benzoindoles heptamethine cyanine dyecontains polyethylene glycol, a benzoindoles ring and a heptamethine chain; a general formula structure of the pegylation benzoindoles heptamethine cyanine dye is a heptamethine chain which containsheptamethine conjugated chain without substituent or is connected with a chlorocyclopentene bridge ring, and chlorine atoms on the chlorocyclopentene bridge ring can be replaced by active groups of sulfydryl, amino and the like to be applied to further dye decoration. The pegylation benzoindoles heptamethine cyanine dye disclosed by the invention has the beneficial effects that a polyethylene glycol chain boned to the benzoindoles ring ensures that the dye has good water solubility and biological compatibility; an aromatic ring, a heterocyclic ring and a methenyl chain can form a large conjugated structure to provide a condition for fluorescent color development of the functional dye; as the functional dye has better water solubility and optical stability, the functional dye can be used aswater-soluble near-infrared fluorescent dye.

The invention relates to a method for separating and purifying vitamin C palmitate from reaction liquid of a direct esterification method, and the method applied to separation and purification fields of the reaction liquid. The separating and purifying method comprises the following steps of: reacting vitamin C with palmitic acid under catalysis of concentrated sulfuric acid, so as to obtain the reaction liquid of the vitamin C palmitate; slowly pouring the reaction liquid into ice-bath cooling water or trash ice, adding methylbenzene to carry out extraction, and carrying out standing and layering to separate out a lower-layer waste acid layer and leave a methylbenzene layer; adding water into the methylbenzene layer, carrying out crystallization and filtering so as to obtain a filter cake, and respectively washing the filter cake by utilizing proper amount of methylbenzene and water; adding the filter cake into the water and stirring for 0.5-3 hours, filtering, and carrying out water washing until the pH of the effluent water is 6-7, so as to obtain crude products of the vitamin C palmitate; and carrying out recrystallization on the crude products of the vitamin C palmitate by utilizing ethanol so as to obtain finished products of the vitamin C palmitate. The method for separating and purifying the vitamin C palmitate from the reaction liquid of the direct esterification method has the advantages that solvents are simply separated and purified, are low in toxin and can be recycled, the separation and purification cost is low, the product purity is high, the condition is mild and the operation is simple and convenient.

The invention discloses a method suitable for industrialized production for separating and purifying natto kinase, which relates to the technical field of separation and extraction of natto kinase for treating thrombus diseases and solves the problems that the conventional natto kinase purification process is complex and is not suitable for industrialized production. A pure natto kinase product is obtained in a separation and purification mode by adopting ethanol precipitation and crude extraction, cation and anion exchange resin chromatography and HPD-400 macroporous absorption resin refining. The common expensive material such as gel and the like is replaced by adopting a column chromatography filling material suitable for industrialized production, the yield can reach 59 percent, and the purity reaches over 95 percent; and the method has the characteristics of high yield, low cost and capability of scale production, basically solves the bottleneck problem of natto kinase separation and purification in the industrialized production, creates a condition for obtaining the natto kinase with high purity and high enzyme activity by efficient separation, and has good economic benefit and social benefit.

The invention discloses an arginine deiminase high-yielding Bacillus cereus, which is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO:M 2015047. The invention also discloses application of the Bacillus cereus in production of L-citrulline and a production method of L-citrulline. The Bacillus cereus provided by the invention can transform over 98% of arginine to L-citrulline, and L-citrulline with purity of more than 99% can be prepared. The method provided by the invention also has the characteristics of low production cost, short period and the like.

A method for separation and purification of LCZ696 intermediate diastereoisomer includes the following steps: (1) placing the mixture of the diastereoisomer (I-a) and (I-b) in an organic solvent untilthe mixture is completely dissolved, cooling to 0-10 DEG C, recrystallizing until the crystal is stable, filtering, taking a filter cake, and drying to obtain a product mixture, and (2) taking the filtrate, removing the solvent under reduced pressure, and drying the solute to obtain an impurity mixture. The method for separation and purification can efficiently and conveniently separate and purify the diastereomers (I-a) and(I-b) and the purity of the resulting product mixture (I-a) is as high as 95 percent, which can be directly used for preparing a high-purity Sacubitril intermediate to finally synthesize LCZ696 so as to ensure the product quality.

The invention discloses a supported ruthenium amorphous alloy catalyst as well as a preparation method and application thereof. According to the catalyst, fumed silica serves as a carrier, ruthenium serves as an active component, sodium borohydride serves as a reducing agent, and the metal ruthenium and the aid boron are dispersed on SiO2 uniformly and firmly in the form of amorphous alloy Ru-B according to a chemical reduction method; the catalyst prepared according to the preparation method has the amorphous form, has large specific surface, is dispersed uniformly and has small particles. When the catalyst is applied to preparation of dihydric alcohol by glycerinum selective hydrogenation, the process is simple, mild in reaction condition, high in activity on the glycerinum, high and stable in selectivity on the glycol and low in separation and purification cost; due to the price advantage and the renewability of the biomass raw materials, the process is favorable for reducing the production cost of the glycol.

The invention relates to a fermentation process for preparing tiancimycin-A and derivatives thereof by streptomyces sp., wherein the streptomyces sp. is streptomyces sp. CB03234-S, is preserved in China Center for Type Culture Collection on September 25, 2017, and has the preservation number of CCTCC M 2017538. By systematically optimizing the fermentation culture medium of the TNM-A high-yieldingstrain CB03234-S, the optimized culture medium suitable for high-efficiency synthesis of the target product TNM-A is determined; on the basis, pilot scale amplification of the microbial fermentationpreparation method is carried out in a fermentation tank, and the microbial fermentation preparation process of the TNM-A is established and optimized by control of the temperature, the rotating speedand the pH value, control of raw material supplementation and other control means; finally, through the gradient control of dissolved oxygen in fermentation broth in the fermentation process (the dissolved oxygen in the fermentation broth is controlled at 60-80% in the early stage of fermentation, the dissolved oxygen in the fermentation broth is controlled at 40-60% in the middle and later stageof fermentation, and the pH is 8.0-9.0) and feeding control, the fermentation yield of the TNM-A is further improved, and the yield of the TNM-A breaks through 20 mg / L.

The invention relates to the field of gene engineering, in particular to a method for efficiently expressing lysostaphin. According to the invention, secretory expression of lysostaphin is carried outby using a pichia pastoris expression system, massive expression of lysostaphin is realized, thus reducing production costs; the highest expression quantity of lysostaphin reaches 6g / L; the lysostaphin is secreted and expressed in the fermentation supernatant, cells do not need to be broken, thalli only need to be removed through simple filtration, and lysostaphin crude enzyme liquid with few impure proteins can be obtained; the lysostaphin sample prepared through the method has an obvious inhibiting effect on staphylococcus, especially staphylococcus aureus.

The invention provides a method for lowering generation of a succinic acid fermentation byproduct, i.e., acetic acid. In the method, in a process that Actinobacillus succinogenes utilizes different carbon sources (glucose, starch hydrolysate and cellulose hydrolysate) to ferment succinic acid, a certain quantity of sodium hydrogen sulfite is added, and the generation of the byproduct, i.e., the acetic acid, can be obviously lowered. When the glucose is taken as a raw material to ferment the succinic acid, after the sodium hydrogen sulfite is added, the yield of the acetic acid can be lowered to 8.18 g / L from 17.85 g / L, and decreasing amplitude is as high as 54.17%. According to the method, the problem that the content of the byproduct, i.e., the acetic acid, is slightly high in a processthat microorganism fermentation is carried out for generating the succinic acid is solved, the separation and purification cost of the succinic acid is lowered, and therefore, the method disclosed bythe invention is favorable for accelerating the development of the industry that the succinic acid is produced by a microorganism fermentation method.

The invention discloses a continuous casting billet secondary cooling method adopting carbon dioxide-water spray cooling, belongs to the field of metallurgy, and solves the problem that an existing secondary cooling technology cannot meet the requirement for high quality developing of continuous cast steel billets. The method comprises the following steps that the carbon dioxide gas is pressurizedto 1.4-1.8 MPa through a compressor, and is stored in a carbon dioxide gas holder; the water is pressurized to 1.4-1.8 MPa through a booster pump, and is stored in a high-pressure water tank; the carbon dioxide gas in the carbon dioxide gas holder is conveyed to a gas distributor through a pipeline, and then is conveyed to a secondary cooling discharging tube by the gas distributor; the water inthe high-pressure water tank is conveyed to a water distributor through the pipeline, and then is conveyed to the secondary cooling discharging tube through the water distributor; and the mixed gas mist is sprayed to the surface of a casting blank of a secondary cooling zone through a atomizing nozzle on the secondary cooling discharge tube to cool the casting blank. According to the device, the oxidation layer on the surface of the casting blank is reduced, so that the metal yield is improved, and the water amount and the gas consumption of the secondary cooling water are reduced.

The invention provides a high-temperature-resistant xylanase secrete expression vector and a method for high-efficiency expression of the high-temperature-resistant xylanase by a lactic acid Kluyveromyces lactis. By adopting the method, most of the xylanase expressed is secreted outside cells, which lowers the cost of separation and purification and greatly improves the expression rate; wherein, under the condition of shaking culture, the amount of the xylanase secreted outside the cells can be up to 70mg / L when galactose is used for induction culture; and when RBB-xylan is taken as a substrate, the activity of the xylanase is up to 6,100U / L.

The invention discloses a preparation method of a cyclodextrin modified magnetic nano adsorbent and the application of the cyclodextrin modified magnetic nano adsorbent in a hemodialysis adsorption system, belonging to the field of biomedical material. The method takes FeCl3.6H2O, FeCl2.4H2O and ammonia water as raw materials, trisodium citrate (TSC) as a dispersing agent, polyethylene imine (PEI) as a modification substrate and beta-cyclodextrin (CD) as an adsorption functional group, and comprises the steps of synthesizing magnetic nanoparticles which are modified by the TSC and are small in particle sizes, good in dispersion and strong in magnetism by a reverse chemical coprecipitation method; enabling a PEI modified layer to be adsorbed onto the surface of Fe3O4-TSC by electrostatic interaction; grafting the beta-CD by a Schiff base reaction to obtain the cyclodextrin modified magnetic nano adsorbent-Fe3O4-beta-CD. The adsorbent has magnetism, so that the separation and purification process is more convenient and rapider, and the separation and purification cost is low; the cyclodextrin modified magnetic nano adsorbent has better adsorption capacity for hydrophobic toxin in the hemodialysis adsorption system.