Method for lowering generation of succinic acid fermentation byproduct, i.e., acetic acid

A technology of succinic acid and by-products, which is applied in the field of reducing the production of acetic acid by-products of Dingersun fermentation, can solve the problem of high production of by-product acetic acid, and achieve the effects of improving raw material utilization, reducing costs, and reducing separation and purification costs

Pending Publication Date: 2020-09-18
GUANGXI UNIV FOR NATITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Aiming at the defect of high yield of by-product acetic acid in the existing fermentation production of succinic acid, the present invention provides a method for reducing the production of acetic acid by-product of succinic acid fermentation

Method used

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  • Method for lowering generation of succinic acid fermentation byproduct, i.e., acetic acid
  • Method for lowering generation of succinic acid fermentation byproduct, i.e., acetic acid

Examples

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Embodiment 1

[0039] A method for reducing succinic acid fermentation by-product acetic acid generation, comprising the following steps:

[0040] (1) Inoculate a single colony of Actinobacillus succinogenes GXAS137 on the plate into the seed medium, and culture it in an anaerobic incubator at 37°C for 16 hours, and the number of bacteria reaches more than 300 million, and the expanded culture strain is obtained;

[0041](2) According to the inoculum amount of 5% (V / V), carry out secondary expansion and cultivation of the expanded culture for 8 hours until the number of bacteria reaches more than 300 million, that is, liquid seeds are obtained;

[0042] (3) According to the inoculum amount of 5% (V / V), inoculate the liquid seeds into a 250mL anaerobic bottle containing the fermentation medium, the liquid volume is 150mL, and add 0.05g / L sodium bisulfite at the same time, Use pH buffer to adjust the pH of the fermentation broth to maintain at 6.5-7.0, and then fill it with N 2 Under the cond...

Embodiment 2

[0050] A method for reducing succinic acid fermentation by-product acetic acid generation, comprising the following steps:

[0051] (1) Inoculate a single colony of Actinobacillus succinogenes GXAS137 on the plate into the seed medium, and culture it in a common incubator at 38°C for 20 hours, and the number of bacteria reaches more than 300 million, and the expanded culture strain is obtained;

[0052] (2) According to the inoculum amount of 5% (V / V), carry out the secondary expansion and cultivation of the expanded culture strains for 8 hours, and the number of bacteria reaches more than 300 million, that is, liquid seeds are obtained;

[0053] (3) Inoculate liquid seeds into a 250mL anaerobic bottle containing fermentation medium at an inoculum volume of 15% (V / V). , use the pH buffer to adjust the pH of the fermentation broth to maintain at 6.5-7.0, when it is filled with CO 2 Under the conditions of rotation speed 200r / min and temperature 38°C, batch fermentation was car...

Embodiment 3

[0060] A method for reducing succinic acid fermentation by-product acetic acid generation, comprising the following steps:

[0061] (1) Inoculate a single colony of Actinobacillus succinogenes GXAS137 on the plate into the seed medium, and culture it in an anaerobic incubator at 37°C for 16 hours, and the number of bacteria reaches more than 300 million, and the expanded culture strain is obtained;

[0062] (2) According to the inoculum amount of 5% (V / V), carry out the secondary expansion and cultivation of the expanded culture strains for 8 hours, and the number of bacteria reaches more than 300 million, that is, liquid seeds are obtained;

[0063] (3) According to the inoculum amount of 5% (V / V), inoculate the liquid seeds into the fermentation medium and ferment in a 2L fermenter with a liquid volume of 1200mL. Add 0.3g / L sodium bisulfite 6h after inoculation , use the pH buffer to adjust the pH of the fermentation broth to maintain at 6.5-7.0, and when it is full of N 2 ...

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Abstract

The invention provides a method for lowering generation of a succinic acid fermentation byproduct, i.e., acetic acid. In the method, in a process that Actinobacillus succinogenes utilizes different carbon sources (glucose, starch hydrolysate and cellulose hydrolysate) to ferment succinic acid, a certain quantity of sodium hydrogen sulfite is added, and the generation of the byproduct, i.e., the acetic acid, can be obviously lowered. When the glucose is taken as a raw material to ferment the succinic acid, after the sodium hydrogen sulfite is added, the yield of the acetic acid can be lowered to 8.18 g/ L from 17.85 g/ L, and decreasing amplitude is as high as 54.17%. According to the method, the problem that the content of the byproduct, i.e., the acetic acid, is slightly high in a processthat microorganism fermentation is carried out for generating the succinic acid is solved, the separation and purification cost of the succinic acid is lowered, and therefore, the method disclosed bythe invention is favorable for accelerating the development of the industry that the succinic acid is produced by a microorganism fermentation method.

Description

technical field [0001] The invention belongs to the technical field of industrial microbial fermentation, and in particular relates to a method for reducing the generation of acetic acid, a by-product of Dingersun fermentation. Background technique [0002] Succinic acid ( Succinic acid ), also known as succinic acid ( Butanedioic acid ), is a common natural organic acid, first isolated from amber by Georgius in 1546, hence the name. The molecular formula of succinic acid is C 4 h 6 o 4 , the molecular weight is 118.09, the taste is sour, the relative density is 1.572, the melting point is 188°C, and the boiling point is 235°C (decomposition temperature). [0003] Succinic acid is an important C4 platform compound, and it is the basic raw material of important bulk chemicals and specialty chemicals such as 1,4-butanediol, tetrahydrofuran, γ-butyrolactone, N-methylpyrrolidone, adipic acid, etc. , widely used in food, plastics, medicine, spices and other industries, can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12N1/20C12N1/38C12R1/01
CPCC12P7/46C12N1/20C12N1/38
Inventor 申乃坤张红岩姜明国王一兵
Owner GUANGXI UNIV FOR NATITIES
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