The invention discloses a biological purifying method for seafood bivalve shellfishes. The method comprises the following steps of: firstly, carrying out semi-fermentation on purple seaweed in a transparent way to obtain the purifying liquor containing purple seaweed colloid; and then, putting the bivalve shellfishes into a purifying tank added with the purifying liquor to purify for 1-3 days, therefore, the bivalve shellfishes can eat the purple seaweed colloid, the purple seaweed colloid can form a colloidal membrane in the digestive tract, the colloidal membrane not only can adsorb the impurities of mud, sand, heavy metal and the like in the digestive tract, and also can adsorb the microorganisms of colon bacillus and the like in the digestive tract, and the mud, the sand, the heavy metal and the microorganisms are discharged along with the purple seaweed colloid, thereby decreasing the content of the mud, the sand, the heavy metal and the microorganisms of the bivalve shellfishes, and achieving the food edible safety standard. In the method, as the biological purifying mode is adopted, the shellfishes can maintain the original freshness and taste; and as the purifying time is short, the relative fatness of the shellfishes is basically not affected, and the customer satisfaction degree and economical benefit of the shellfishes are not decreased.