177results about How to "Reduce purification time" patented technology

Method for producing highly purified silicon for use in solar cells by a single solidification purification, pouring silicon into a mold and gradually fractionally solidifying it while solidifying the liquid surface, followed by purifying the solidified silicon by zone melting or continuous casting using an electromagnetic mold, or by zone melting in combination with continuous casting, and optionally causing directional solidification to concentrate impurities, leaching and recycling.

The invention relates to a method for clean purifying L-tryptophan by utilizing fermented liquid, which is applied in the purification of L-tryptophan. The method sequentially comprises the following steps of (1.1) microfiltration, (1.2) ultrafiltration, (1.3) nanofiltration, (1.4) cryoconcentration, (1.5) conventional centrifugal separation to obtain crude products and take the produced filtrate as mother liquid which is returned to (1.1) microfiltration for recycling, (2.1) preparation of 35% alcohol solution of L-tryptophan crude products to be purified, (2.2) decolorization of active carbon, (2.3) filtration, (2.4) cooling, mixing, crystallization and centrifugation and (2.5) conventional procedures of washing, drying and packaging; the filtration in (2.3) is conventional filtration by plate frames; and the washing in (2.5) adopts alcohol aqueous solution for washing and returns filtrate and washing liquid produced therefrom as the mother liquid to (2.2) decolorization of active carbon for recycling. The method has the advantages of recycling of the mother liquid, short purification time, high purification purity, cost saving and the like.

The invention discloses a production method for extracting tryptophan from fermentation liquor by one-step refining, which comprises the pretreatment of the fermentation liquor. In the method, mycoprotein is filtered off by using a ceramic microfiltration membrane, is decolorized by using an ultrafiltration membrane and is desalted and concentrated by using a nanofiltration membrane; the nanofiltration membrane concentrated liquor is directly decolorized by using activated carbon, the decolorized liquor is subjected to isoelectric point crystallization at a low temperature, and a qualified competitive product is obtained by rinsing with ice water during centrifugal separation; the crystallization mother liquor returns to the nanofiltration membrane for circulation; protein feed with high additional value is prepared by mixing, drying and crushing the ultrafiltration strong liquor and mycoprotein; and nanofiltration strong brine can be used as a culture medium for later use or sold serving as a base fertilizer. The method has the advantages of avoiding adding an organic solvent and an inorganic solvent in the preparation process of a crude product and a fine product, saving the solvent recovery cost, solving the problems of sewage treatment and environmental pollution, along with high utilization rate of the mother liquor, high product quality, and more contribution to industrial production, wherein the total extraction yield of L-tryptophan is 83.5 percent in a 500-ton production line.

The present invention relates to a biological sample collection, archiving, purification, and manipulating system and methods for collecting, archiving, purifying, and manipulating biological samples. The system can include a plurality of devices, each having a species-immobilizing filter within a tubular body. The body includes first and second end openings and one or more removable caps or sealing devices for sealing the end openings. A plurality of the devices can be positioned in an array tray for simultaneously archiving, purifying, or reacting respective samples collected in the respective devices. In another embodiment, a plate system is provided that includes a plate having a plurality of through-holes with each through-hole having a species-immobilizing filter disposed therein. The plate system also includes sealing devices such as sealing trays or removable caps for sealing first and second end openings of each through-hole of the plate.

The invention belongs to the technical field of physical metallurgy technological purification, and in particular relates to a method and equipment for purifying polycrystalline silicon through electron beam melting. A high-phosphorus silicon material is molten through electron beams under the vacuum condition to obtain low-phosphorous molten silicon, and the low-phosphorous molten silicon is poured into a solidification crucible to be solidified; the processes of charging, smelting and purifying and pouring are repeated until the solidification crucible is fully loaded; another hollow solidification crucible is rotated below a molten liquid pouring port, and the processes of charging, smelting and purifying and pouring are repeated until the solidification crucible is fully loaded; and the processes of fully charging one solidification crucible and rotating another hollow solidification crucible below the molten silicon pouring port are repeated until all the solidification crucibles are fully loaded, and after the low-phosphorous molten silicon in all the solidification crucibles is solidified, ingots are taken out. By the method, the whole purifying time of a plurality of times of smelting is reduced, and the times for vacuumizing and preheating of electron guns are reduced, so that the production efficiency is increased; and the equipment is compact in structure, is easy to operate, is safe and controllable and has high production efficiency.

The invention belongs to the technical field of genetic engineering and discloses a new method for xylanase secretory expression in escherichia coli. A signal peptide is fused to the N terminal of a xylanase gene, meanwhile, a codon of an encoding histidine tag is added to the C terminal of a xylanase gene sequence, and a target gene nucleotide sequence Signal peptide-Xylnase-6*His is obtained through PCR product recovery; in a target gene cloning and expression vector pET28a, a correct recombinant expression vector is obtained through sequencing; the recombinant expression vector is convertedto a escherichia coli strain; IPTG is added into a sample, and induction culture is carried out on the sample; efficient secretory expression of xylanase is achieved. According to the method, xylanase can be directly secreted into a culture medium; compared with original escherichia coli intracellular expression or yeast secretory expression, the high activity of xylanase can be ensured, the operation is easier, more time is saved, and the cost is lower.

The invention provides a method for quickly transforming a cytoplasmic male sterile line of brassica vegetables, which comprises the steps of: using a brassica breeding material with the cytoplasmic male sterile line as a female parent, and using pure brassica breeding material with genome type different from that of the female parent as a male parent for interspecies cross, obtaining interspecies cross F1 plant by an embryo rescue technology, then backcrossing with the male parent continuously, and quickly obtaining the cytoplasmic male sterile line through field selection and chromosome identification. The method has the beneficial effects that: 1) the breeding process of the cytoplasmic male sterile line of brassica vegetables is accelerated by interspecies cross characteristics of three genomes of cabbage, leaf mustard and wild cabbage in the brassica vegetables; 2) flowering cabbage or wild cabbage is selected as the source of cytoplasmic male sterile resource, so that a quick transforming method system which covers the cytoplasmic male sterile lines of all genome types of the brassica vegetables is formed; and 3) flowering cabbage or wild cabbage directly bolts and blossoms without vernalization, so that the sterile line transforming time is shortened.

The invention relates to the field of compound extraction and purification, in particular to a purification method of rhubarb stilbene glucoside. The purification method comprises the following steps: taking rheum lhasaense, extracting with ethanol and carrying out non-polar macro-porous resin first purification; eluting with ethanol water solution, collecting a first eluent and carrying out polar resin or non-polar resin second purification of a styrene framework structure; and eluting with ethanol water solution with a concentration of 80-95 percent, collecting a second eluent and refining. The method has the advantages of simplicity and convenience in operation, easiness in reproduction, shortened purification and reduced energy consumption, and the purity of rhubarb stilbene glucoside reaches above 98 percent.

The invention relates to an antibody acidic peak purification method. During purification of the antibody acidic peak, sodium phenylbutyrate is added so that a good effect is achieved in application of purification of the antibody acidic peak. During purification of the TNFaplha antibody, content of acidic peak of the antibody can be reduced obviously by means of elution in two steps through buffer solution which contains sodium phenylbutyrate and has conductivity changing from low to high. Therefore, the method has remarkable practical industrial use.

The invention discloses an 18F-labeled compound and a pod protease targeted PET imaging probe, which relates to the technical fields of radiopharmaceuticals and nuclear medicine. The invention provides a compound shown as a formula (I), asparagine site shearing and disulfide bond reduction are carried out in a tumor microenvironment with high expression of pod protease, and a radioactive dimer 18F-1-dimer is formed by virtue of a biocompatible CBT-Cys click condensation reaction, so that the tumor imaging effect is improved. The compound with a structure shown in the formula (I) is synthesizedby adopting a one-step ion exchange 18F labeling method, the method is simple to operate, and preparative HPLC for further purification is not needed. The invention further provides the pod proteasetargeted PET imaging probe which is a compound shown in (I). The PET imaging probe has the advantages of high stability, high sensitivity, strong specificity, good safety and the like.

The invention discloses an aseptic collagen liquid with biological activity and a preparation method thereof. The preparation method of the aseptic collagen liquid comprises degreasing, slicing, disinfecting and crushing at a low temperature animal tendons, carrying out acid dissolution and enzymolysis, carrying out filtration to remove residues, and carrying out salting-out, purification, loading, sealing, refrigeration and low temperature irradiation sterilization to obtain the aseptic collagen liquid. The preparation method realizes low temperature irradiation, keeps collagen activity, and prevents collagen inactivation and denaturation or cross-linking reaction under irradiation so that functions are not influenced. The preparation method utilizes ultrafiltration purification to replace the traditional dialysis purification, shortens purification time, utilizes an automatic system to replace the traditional semi-automatic operation, prevents human hand pollution and guarantees product cleanliness, purity and bioactivity.

The invention discloses an industrial gardenia yellow pigment purification device and a method for extracting the gardenia yellow pigment. The device comprises an elution column, a vacuum recycling tank, a condenser, a dehydration column and a solvent heater, wherein the elution column, the vacuum recycling tank, the condenser, the dehydration column and the solvent heater are sequentially communicated with one another by virtue of pipelines; a sampling pipe is communicated with a pipeline between the elution column and the vacuum recycling tank; a sampling valve is arranged on the sampling pipe; a vacuum pump is arranged at the outlet of the dehydration column; a collection pipe is communicated with a pipeline between the dehydration column and the solvent heater; a collection valve is arranged on the collection pipe; the dehydration column is filled with dehydrating agents; and a water bath heating jacket is arranged outside the vacuum recycling tank in a sleeving manner. The method comprises the step of purifying gardenia yellow pigment from fine powder of solid gardenia yellow pigments containing lots of impurities. The device is simple in structure and convenient to use and is suitable for industrial production. The method is simple, low in cost, clean and environmentally friendly. The gardenia yellow pigment obtained by using the method is small in losses, high in purity and high in color value.

The invention discloses a method for preparing chondroitin sulfate from sturgeon cartilage. The method comprises the following steps: (1) pretreating cartilage; (2) performing ultrasonic enzymolysis treatment: hydrolyzing the sturgeon cartilage by using trypsin, accelerating the enzymolysis process under the action of ultrasonic waves, finishing enzymolysis, deactivating enzyme, cooling and centrifuging to obtain supernate containing sturgeon chondroitin; (3) hydrolyzing and precipitating synchronously, continuously hydrolyzing the supernate in the step (2) with ethanol and NaOH solution and centrifuging to obtain white precipitate; and (4) purifying, drying and redissolving the precipitate to remove insoluble impurities, and drying to obtain a white spongy chondroitin sulfate product. The process is simple to operate and is new. Based on the steps of performing ultrasonic auxiliary enzymolysis, hydrolyzing and precipitating synchronously and purifying, the hydrolysis time can be greatly shortened, the purity and the yield of the products are increased and the production cost is reduced.

The invention discloses a graphite purification method which comprises the following steps: S1, preparing graphite powder, uniformly mixing the graphite powder, screening, and performing impurity removal treatment so as to obtain a graphite powder raw material to be purified; S2, spreading the graphite powder raw material to be purified on a heating platform so as to form a graphite powder material layer; S3, setting a laser emitter above the heating platform, performing continuous transverse scanning on the graphite powder material layer by using the laser emitter, performing radiation heating, and gasifying impurities in the graphite powder raw material; S4, cooling, thereby obtaining graphite of which the solid carbon content is 99.9-99.99%. By adopting a laser radiation heating method,impurities of the graphite powder raw material are volatilized in a gas mode on premise that the graphite self is not affected, high-purity graphite is obtained, energy consumption and cost are greatly reduced, in addition, the laser heating temperature is up to 3000 DEG C or greater, impurities can be effectively and rapidly gasified, and the purification efficiency is improved.

The invention discloses a purification production process of glycerol. The process comprises the steps as follows: crude glycerol is sucked into a distillation still, direct steam is fed when the crude glycerol is heated to the temperature of 160+ / -5 DEG C, and theintake amount of the direct steam is controlled to the extent that system vacuum is not lower than 0.08-0.095 MPa; glycerol steam obtained during distillation sequentially passes through a first condenser, a second condenser, a third condenser and a fourth condenser for cooling condensation to obtain distilled glycerol; the weight of the distilled glycerol is M, the distilled glycerol is input into a decoloration tank and heated to the temperature of 90+ / -5 DEG C, then activated carbon is added, the weight of the activated carbon is M*(0.1%-0.5%), the distilled glycerol is fully stirred for 25-35 min, the temperature is kept for 0.5-1.5 h, the glycerol is obtained through filtration, and the filtration pressure is lower than 0.4 MPa. The purification production process of the glycerol is simple in process and thorough in purification process, and the obtained glycerol contains few impurities and is high in yield.

The invention discloses engineering bacteria of soluble expression Not I, and a construction method and application thereof. The engineering bacteria of the invention comprises methylase Eag I M gene, restriction enzyme Not I gene and escherichia coli (E. coli) ER2566 (mcrC-mrr). After separating and identifying the Not I gene, the invention obtains the engineering bacteria of efficient soluble expression Not I through the reconstruction of the recombinant. In the invention, the Not I restriction enzyme with high purity can be prepared through groping the inducement conditions of recombining the engineering bacteria and optimizing a protein purification process.

The invention relates to a nucleic acid recovery and purification kit which comprises a sol solution, a magnetic bead solution and a combination solution. The sol solution contains 3-4 mol / L guanidine isosulfocyanate, 20-30 mol / L Tris-HCl buffer solution and the balance of water. The magnetic bead solution contains 0.2-3 mg / mL hydroxy magnetic bead and the balance of water. The combination solution contains 40-60% isopropanol, 1-2 mol / L sodium chloride and the balance of water. The invention also relates to a nucleic acid recovery and purification method using the kit. The nucleic acid recovery and purification kit and method can quickly and efficiently recover and purify large-segment nucleic acids in a sepharose gel.

The invention relates to the technical field of collagen purification, in particular to a method for purifying type I collagen. The method for purifying the type I collagen comprises the following steps: S1: adopting a proper method to extract the type I collagen; S2: obtaining a type I collagen dilute solution; S3: obtaining the purified type I collagen. Compared with the prior art, the method for purifying the type I collagen has the advantages that the purification time of the type I collagen can be obviously shortened, and an ideal purification effect can be achieved. According to the method, only 12-24 hours are required for an ultrafiltration purification technology to purify the collagen to achieve required purification and concentration, but time of about one week is required for the dialysis method purification of the prior art to achieve the same purity and concentration. The method for purifying the type I collagen has the characteristics of simple method, convenience in operation and low production cost and can be suitable for industrial large-scale application.

The invention relates to a CYP101 enzyme recombinant vector, a construction method thereof and a CYP101 enzyme high-efficiency expression and purification method, and belongs to the field of biotechnology. A PET28a-CYP101 high-efficiency expression vector is constructed and transformed into BL21 plysS strain, and high-purity enzyme with purity higher than 95% is obtained by a simple two-step purification method including affinity chromatography and ion exchange chromatography after inducible expression. Compared with the prior art, CYP101 is simply and efficiently produced, the cost is low, purification is facilitated, and 23mg of target protein with the purity higher than 95% can be obtained by per liter of culture media. The CYP101 enzyme high-efficiency expression and purification method can be used for expressing and purifying CYP101 enzyme, the production efficiency of a laboratory is greatly improved, and experimental cost is greatly reduced. The CYP101 enzyme high-efficiency expression and purification method has an excellent application prospect for large-scale CYP101 enzyme production and purification.

The invention discloses a sterile collagen dressing and a preparation method thereof. The preparation method of the sterile collagen dressing comprises the following steps: degreasing an animal tendon, slicing, disinfecting, smashing, performing acid soluble enzymolysis, centrifuging and removing the precipitate to obtain a collagen stock solution; adjusting the concentration and the viscosity of the collagen stock solution, performing ultrafiltration and purification, and condensing to obtain the collagen solution with high purity and high concentration; performing freeze drying, smashing and performing irradiation sterilization, dosing and filling into an aluminum foil bag containing a non-woven. The activity of the collagen is kept, and the degeneration of the collagen due to the irradiation or the cross-linking reaction can be avoided; the traditional dialysis purification is replaced by the ultrafiltration and purification so that the purifying time is shortened; the traditional semiautomatic operation is replaced by an automatic system, so that the hand pollution is avoided, and the cleanliness, purity and biological activity of the product are guaranteed.

The invention refers to the constructive key technology, construction result and the purifying method for producing a-2b interferon of yeast project bacterial strain IFN a-2b / pGAPZa-A / GS115 which can excrete a-2b interferon. The invention uses yeast secretion expressing system as a substitute for colibacillus expressing system, the purpose albumen a-2b interferon needn't repetitiveness, the biological rate activity reaches to 1.0X10 to the 9th power unit / mg albumen, it has no side or noxious effect, and the purification can reach to 95%.

The invention provides a high-efficiency combined extraction method of high-purity purslane polysaccharide and total flavone, and relates to a method for deproteinizing and decoloring an extracting solution. The high-efficiency combined extraction method can achieve efficient and rapid extraction of purslane polysaccharide and total flavone from portulaca oleracea L. in a large scale, and single-step operation of specific micellar medium extraction is adopted for jointly removing protein and pigment components in a polysaccharide extraction step, and correspondingly the defect is avoided thatprotein removal and pigment removal need to be independently carried out during purslane polysaccharide purification in the prior art; the extraction flow is shortened, and the method is suitable forlarge-scale extraction.

The invention discloses a method of purifying Bt toxalbumin from insect-resistant cotton seeds. The invention provides a method of purifying Bt Cry1Ac protein from insect-resistant cotton seeds. The method comprises the following steps: (1) peeling the insect-resistant cotton seeds and then extracting total soluble protein to obtain a solution A; (2) conducting ammonium sulfate precipitation to the solution A, and centrifugally collecting the precipitate, wherein the saturation of the ammonium sulfate is 60%; (3) dissolving the precipitate obtained from the step (2), and dialyzing to obtain a solution B; and (4) conducting immuno-affinity chromatography to the solution B, collecting protein components, namely Bt toxalbumin, combined with the specificity of anti-Bt Cry1Ab / Ac protein monoclonal antibody, wherein the anti-Bt Cry1Ab / Ac protein monoclonal antibody is produced by secreting hybridoma Bt2F9 CGMCC No.5162. The method has the following advantages that the purification procedures are simplified, the purification cost is lowered, the purification time is shortened and reducing the loss of target proteins is reduced.

A catalytic apparatus for a vehicle, may include a caning that is a single hollow unit with both distal ends open, a front substrate and a rear substrate that are disposed apart from each other with a predetermined space therebetween in the caning, and a front flange that is connected to one of the distal ends of the caning to be joined with a front muffler pipe connected to an exhaust manifold and has a bottom portion inclined toward the center axis of the front substrate in the vertical cross section.

The invention provides a purifying method of ionic liquid. The purifying method comprises a step that: the ionic liquid is purged by using inert gas, such that volatile impurities in the ionic liquid are removed. With the method, volatile substances in synthesized or recovered ionic liquid containing volatile impurities can be removed in a relatively short time, such that the purification of the ionic liquid is realized. Compared to traditional methods, with the method provided by the invention, the purification time can be shortened, the energy consumption during the purification period can be reduced, and the degradation of the ionic liquid can be avoided.

The invention discloses a purification method for blackberry anthocyanin. The purification method includes the steps of resin pretreatment and blackberry anthocyanin crude extract purification. Efficient purification of the blackberry anthocyanin can be achieved, and the purity of the obtained blackberry anthocyanin is high. According to the purification method, polyamide resin is selected as adsorbent and has the advantages that the large specific area and the large granularity are achieved, adsorption and desorption capacity is high, cost is low, regeneration is easy, and a preparation method is simple. Through research on the static and dynamic adsorption and desorption process of the blackberry anthocyanin, purification conditions are optimized, the initial concentration of the blackberry anthocyanin, the pH value of adsorption liquid, the pH value of eluent and the optimal value of ethanol concentration are determined, and the adsorption rate and the elution rate of the blackberry anthocyanin are both high. According to the method, time-saving and efficient purification of the blackberry anthocyanin can be achieved, purification time can be shortened, and cost can be reduced.

The invention relates to a method for silver nanowire purification through vibration and sedimentation and belongs to the technical field of silver nanowire purification. The technical problems that an existing method for silver nanowire purification is large in acetone dosage and poor in removal effect of impurities such as particles are solved. The method comprises the steps that 1, acetone is added into a silver nanowire mother solution prepared through a polyol method, vibration is conducted, and an upper solution is removed; 2, then distilled water is added, vibration is conducted till the nanowire is completely dispersed, acetone is added again, vibration is conducted, and an upper solution is removed; and 3, if the upper solution is muddy, operation of the step two is repeated till an upper solution is completely clear and transparent, the upper solution is removed, and silver nanowire purification is completed. According to the method for silver nanowire purification through vibration and sedimentation, acetone adding and vibration are combined, the impurities such as the particles in a nanowire solution are separated step by step, purity of the silver nanowire is greatly improved, the acetone dosage is reduced, still standing is not needed, treating time is shortened, the operation steps are few, complex devices are not needed, and the method has an obvious cost advantage.

The invention discloses a refining process of a biodiesel by-product, namely crude glycerol. The refining process comprises the following steps: adding an acid solution into the crude glycerol and acidifying until the pH (Potential of Hydrogen) is 3 to 4; after heating to 30 DEG C to 50 DEG C, stirring for 10min to 30min; after standing and layering, removing upper-layer fatty acid; adding water and diluting until the water content is 25 weight percent to 35 weight percent; adding an efficient alkalization adsorbent and stirring for 30min to 60min; then standing and filtering; pre-heating to 60 DEG C to 80 DEG C; adding an alkaline solution and alkalifying until the pH is 6.5 to 7.5 and filtering; finally, conveying the solution into a distillation kettle and decompressing and distilling, wherein the vacuum degree is 0.075MPa to 0.095MPa; after collecting a fraction at 160 DEG C to 180 DEG C, cooling and condensing to obtain a refined glycerol finished by-product. The refining process of the biodiesel by-product, namely the crude glycerol, provided by the invention, can be used for efficiently preparing the glycerol finished by-product with the purity which is 99.5 percent or more; the production cost can be effectively reduced, and energy sources and resources are saved.

The invention discloses a method for separation and purification of a nanobody, and relates to the technical field of biology. The method comprises the following steps: (1) precipitating impure protein at low pH: regulating pH of a to-be-purified sample to acidity with an acid solution, after standing, performing centrifugation to remove precipitates, and keeping a supernatant solution; (2) precipitating the impure protein at high temperature: heating the supernatant solution obtained in step (1), after heat-preservation standing, performing centrifugation to remove precipitates, and keeping asupernatant solution; and (3) removing the impure protein with an ultrafiltration method: performing ultrafiltration on the supernatant solution obtained in step (2), wherein a protein solution permeating through an ultrafiltration membrane is the high-purity nanobody. The method provided by the invention does not affect the activity of the nanobody, and has simple operation, low cost and a wideapplication range.

The invention discloses a method for improving homologous recombination efficiency and recombination virus screening of pseudorabies virus. According to the method, a pseudorabies virus AH strain transfer vector containing an EGFP gene complete expression box is subjected to enzyme digestion linearization, and then subjected to homologous recombination with the pseudorabies virus AH strain; and the obtained recombination virus screening method comprises the steps of separating fluorescent single host cells with pathological changes, and then performing further screening by improved plaque experiment. After recombinant plasmid enzyme is subjected to digestion linearization, the enzyme is subjected to cell transfection; next, virus homologous recombination is performed, so that the homologous recombination efficiency is improved; by virtue of the method in combination with single cell absorption and plaque purification, the screening and purifying time of the recombination viruses is greatly shortened; and therefore, the method is of great significance to improvement of the virus homologous recombination efficiency, has high application prospect in single cell separation, particularly recombination virus screening, and can be widely applied to the recombination virus screening.