177results about How to "Reduce purification time" patented technology

The present invention relates to a biological sample collection, archiving, purification, and manipulating system and methods for collecting, archiving, purifying, and manipulating biological samples. The system can include a plurality of devices, each having a species-immobilizing filter within a tubular body. The body includes first and second end openings and one or more removable caps or sealing devices for sealing the end openings. A plurality of the devices can be positioned in an array tray for simultaneously archiving, purifying, or reacting respective samples collected in the respective devices. In another embodiment, a plate system is provided that includes a plate having a plurality of through-holes with each through-hole having a species-immobilizing filter disposed therein. The plate system also includes sealing devices such as sealing trays or removable caps for sealing first and second end openings of each through-hole of the plate.

The invention belongs to the technical field of physical metallurgy technological purification, and in particular relates to a method and equipment for purifying polycrystalline silicon through electron beam melting. A high-phosphorus silicon material is molten through electron beams under the vacuum condition to obtain low-phosphorous molten silicon, and the low-phosphorous molten silicon is poured into a solidification crucible to be solidified; the processes of charging, smelting and purifying and pouring are repeated until the solidification crucible is fully loaded; another hollow solidification crucible is rotated below a molten liquid pouring port, and the processes of charging, smelting and purifying and pouring are repeated until the solidification crucible is fully loaded; and the processes of fully charging one solidification crucible and rotating another hollow solidification crucible below the molten silicon pouring port are repeated until all the solidification crucibles are fully loaded, and after the low-phosphorous molten silicon in all the solidification crucibles is solidified, ingots are taken out. By the method, the whole purifying time of a plurality of times of smelting is reduced, and the times for vacuumizing and preheating of electron guns are reduced, so that the production efficiency is increased; and the equipment is compact in structure, is easy to operate, is safe and controllable and has high production efficiency.

The invention relates to an antibody acidic peak purification method. During purification of the antibody acidic peak, sodium phenylbutyrate is added so that a good effect is achieved in application of purification of the antibody acidic peak. During purification of the TNFaplha antibody, content of acidic peak of the antibody can be reduced obviously by means of elution in two steps through buffer solution which contains sodium phenylbutyrate and has conductivity changing from low to high. Therefore, the method has remarkable practical industrial use.

The invention discloses an 18F-labeled compound and a pod protease targeted PET imaging probe, which relates to the technical fields of radiopharmaceuticals and nuclear medicine. The invention provides a compound shown as a formula (I), asparagine site shearing and disulfide bond reduction are carried out in a tumor microenvironment with high expression of pod protease, and a radioactive dimer 18F-1-dimer is formed by virtue of a biocompatible CBT-Cys click condensation reaction, so that the tumor imaging effect is improved. The compound with a structure shown in the formula (I) is synthesizedby adopting a one-step ion exchange 18F labeling method, the method is simple to operate, and preparative HPLC for further purification is not needed. The invention further provides the pod proteasetargeted PET imaging probe which is a compound shown in (I). The PET imaging probe has the advantages of high stability, high sensitivity, strong specificity, good safety and the like.

The invention discloses an industrial gardenia yellow pigment purification device and a method for extracting the gardenia yellow pigment. The device comprises an elution column, a vacuum recycling tank, a condenser, a dehydration column and a solvent heater, wherein the elution column, the vacuum recycling tank, the condenser, the dehydration column and the solvent heater are sequentially communicated with one another by virtue of pipelines; a sampling pipe is communicated with a pipeline between the elution column and the vacuum recycling tank; a sampling valve is arranged on the sampling pipe; a vacuum pump is arranged at the outlet of the dehydration column; a collection pipe is communicated with a pipeline between the dehydration column and the solvent heater; a collection valve is arranged on the collection pipe; the dehydration column is filled with dehydrating agents; and a water bath heating jacket is arranged outside the vacuum recycling tank in a sleeving manner. The method comprises the step of purifying gardenia yellow pigment from fine powder of solid gardenia yellow pigments containing lots of impurities. The device is simple in structure and convenient to use and is suitable for industrial production. The method is simple, low in cost, clean and environmentally friendly. The gardenia yellow pigment obtained by using the method is small in losses, high in purity and high in color value.

The invention discloses a graphite purification method which comprises the following steps: S1, preparing graphite powder, uniformly mixing the graphite powder, screening, and performing impurity removal treatment so as to obtain a graphite powder raw material to be purified; S2, spreading the graphite powder raw material to be purified on a heating platform so as to form a graphite powder material layer; S3, setting a laser emitter above the heating platform, performing continuous transverse scanning on the graphite powder material layer by using the laser emitter, performing radiation heating, and gasifying impurities in the graphite powder raw material; S4, cooling, thereby obtaining graphite of which the solid carbon content is 99.9-99.99%. By adopting a laser radiation heating method,impurities of the graphite powder raw material are volatilized in a gas mode on premise that the graphite self is not affected, high-purity graphite is obtained, energy consumption and cost are greatly reduced, in addition, the laser heating temperature is up to 3000 DEG C or greater, impurities can be effectively and rapidly gasified, and the purification efficiency is improved.

The invention discloses a purification production process of glycerol. The process comprises the steps as follows: crude glycerol is sucked into a distillation still, direct steam is fed when the crude glycerol is heated to the temperature of 160+/-5 DEG C, and theintake amount of the direct steam is controlled to the extent that system vacuum is not lower than 0.08-0.095 MPa; glycerol steam obtained during distillation sequentially passes through a first condenser, a second condenser, a third condenser and a fourth condenser for cooling condensation to obtain distilled glycerol; the weight of the distilled glycerol is M, the distilled glycerol is input into a decoloration tank and heated to the temperature of 90+/-5 DEG C, then activated carbon is added, the weight of the activated carbon is M*(0.1%-0.5%), the distilled glycerol is fully stirred for 25-35 min, the temperature is kept for 0.5-1.5 h, the glycerol is obtained through filtration, and the filtration pressure is lower than 0.4 MPa. The purification production process of the glycerol is simple in process and thorough in purification process, and the obtained glycerol contains few impurities and is high in yield.

The invention discloses engineering bacteria of soluble expression Not I, and a construction method and application thereof. The engineering bacteria of the invention comprises methylase Eag I M gene, restriction enzyme Not I gene and escherichia coli (E. coli) ER2566 (mcrC-mrr). After separating and identifying the Not I gene, the invention obtains the engineering bacteria of efficient soluble expression Not I through the reconstruction of the recombinant. In the invention, the Not I restriction enzyme with high purity can be prepared through groping the inducement conditions of recombining the engineering bacteria and optimizing a protein purification process.

The invention relates to a nucleic acid recovery and purification kit which comprises a sol solution, a magnetic bead solution and a combination solution. The sol solution contains 3-4 mol/L guanidine isosulfocyanate, 20-30 mol/L Tris-HCl buffer solution and the balance of water. The magnetic bead solution contains 0.2-3 mg/mL hydroxy magnetic bead and the balance of water. The combination solution contains 40-60% isopropanol, 1-2 mol/L sodium chloride and the balance of water. The invention also relates to a nucleic acid recovery and purification method using the kit. The nucleic acid recovery and purification kit and method can quickly and efficiently recover and purify large-segment nucleic acids in a sepharose gel.

The invention relates to the technical field of collagen purification, in particular to a method for purifying type I collagen. The method for purifying the type I collagen comprises the following steps: S1: adopting a proper method to extract the type I collagen; S2: obtaining a type I collagen dilute solution; S3: obtaining the purified type I collagen. Compared with the prior art, the method for purifying the type I collagen has the advantages that the purification time of the type I collagen can be obviously shortened, and an ideal purification effect can be achieved. According to the method, only 12-24 hours are required for an ultrafiltration purification technology to purify the collagen to achieve required purification and concentration, but time of about one week is required for the dialysis method purification of the prior art to achieve the same purity and concentration. The method for purifying the type I collagen has the characteristics of simple method, convenience in operation and low production cost and can be suitable for industrial large-scale application.

The invention refers to the constructive key technology, construction result and the purifying method for producing a-2b interferon of yeast project bacterial strain IFN a-2b/pGAPZa-A/GS115 which can excrete a-2b interferon. The invention uses yeast secretion expressing system as a substitute for colibacillus expressing system, the purpose albumen a-2b interferon needn't repetitiveness, the biological rate activity reaches to 1.0X10 to the 9th power unit/mg albumen, it has no side or noxious effect, and the purification can reach to 95%.

A catalytic apparatus for a vehicle, may include a caning that is a single hollow unit with both distal ends open, a front substrate and a rear substrate that are disposed apart from each other with a predetermined space therebetween in the caning, and a front flange that is connected to one of the distal ends of the caning to be joined with a front muffler pipe connected to an exhaust manifold and has a bottom portion inclined toward the center axis of the front substrate in the vertical cross section.

The invention provides a purifying method of ionic liquid. The purifying method comprises a step that: the ionic liquid is purged by using inert gas, such that volatile impurities in the ionic liquid are removed. With the method, volatile substances in synthesized or recovered ionic liquid containing volatile impurities can be removed in a relatively short time, such that the purification of the ionic liquid is realized. Compared to traditional methods, with the method provided by the invention, the purification time can be shortened, the energy consumption during the purification period can be reduced, and the degradation of the ionic liquid can be avoided.

The invention relates to a method for silver nanowire purification through vibration and sedimentation and belongs to the technical field of silver nanowire purification. The technical problems that an existing method for silver nanowire purification is large in acetone dosage and poor in removal effect of impurities such as particles are solved. The method comprises the steps that 1, acetone is added into a silver nanowire mother solution prepared through a polyol method, vibration is conducted, and an upper solution is removed; 2, then distilled water is added, vibration is conducted till the nanowire is completely dispersed, acetone is added again, vibration is conducted, and an upper solution is removed; and 3, if the upper solution is muddy, operation of the step two is repeated till an upper solution is completely clear and transparent, the upper solution is removed, and silver nanowire purification is completed. According to the method for silver nanowire purification through vibration and sedimentation, acetone adding and vibration are combined, the impurities such as the particles in a nanowire solution are separated step by step, purity of the silver nanowire is greatly improved, the acetone dosage is reduced, still standing is not needed, treating time is shortened, the operation steps are few, complex devices are not needed, and the method has an obvious cost advantage.

The invention discloses a refining process of a biodiesel by-product, namely crude glycerol. The refining process comprises the following steps: adding an acid solution into the crude glycerol and acidifying until the pH (Potential of Hydrogen) is 3 to 4; after heating to 30 DEG C to 50 DEG C, stirring for 10min to 30min; after standing and layering, removing upper-layer fatty acid; adding water and diluting until the water content is 25 weight percent to 35 weight percent; adding an efficient alkalization adsorbent and stirring for 30min to 60min; then standing and filtering; pre-heating to 60 DEG C to 80 DEG C; adding an alkaline solution and alkalifying until the pH is 6.5 to 7.5 and filtering; finally, conveying the solution into a distillation kettle and decompressing and distilling, wherein the vacuum degree is 0.075MPa to 0.095MPa; after collecting a fraction at 160 DEG C to 180 DEG C, cooling and condensing to obtain a refined glycerol finished by-product. The refining process of the biodiesel by-product, namely the crude glycerol, provided by the invention, can be used for efficiently preparing the glycerol finished by-product with the purity which is 99.5 percent or more; the production cost can be effectively reduced, and energy sources and resources are saved.

The invention discloses a method for separation and purification of a nanobody, and relates to the technical field of biology. The method comprises the following steps: (1) precipitating impure protein at low pH: regulating pH of a to-be-purified sample to acidity with an acid solution, after standing, performing centrifugation to remove precipitates, and keeping a supernatant solution; (2) precipitating the impure protein at high temperature: heating the supernatant solution obtained in step (1), after heat-preservation standing, performing centrifugation to remove precipitates, and keeping asupernatant solution; and (3) removing the impure protein with an ultrafiltration method: performing ultrafiltration on the supernatant solution obtained in step (2), wherein a protein solution permeating through an ultrafiltration membrane is the high-purity nanobody. The method provided by the invention does not affect the activity of the nanobody, and has simple operation, low cost and a wideapplication range.

The invention discloses a method for improving homologous recombination efficiency and recombination virus screening of pseudorabies virus. According to the method, a pseudorabies virus AH strain transfer vector containing an EGFP gene complete expression box is subjected to enzyme digestion linearization, and then subjected to homologous recombination with the pseudorabies virus AH strain; and the obtained recombination virus screening method comprises the steps of separating fluorescent single host cells with pathological changes, and then performing further screening by improved plaque experiment. After recombinant plasmid enzyme is subjected to digestion linearization, the enzyme is subjected to cell transfection; next, virus homologous recombination is performed, so that the homologous recombination efficiency is improved; by virtue of the method in combination with single cell absorption and plaque purification, the screening and purifying time of the recombination viruses is greatly shortened; and therefore, the method is of great significance to improvement of the virus homologous recombination efficiency, has high application prospect in single cell separation, particularly recombination virus screening, and can be widely applied to the recombination virus screening.