Method for preparing chondroitin sulfate from sturgeon cartilage
A technology of chondroitin sulfate and cartilage, which is applied in the field of seafood processing, can solve the problems of unfavorable industrial production of chondroitin sulfate, low purity of chondroitin sulfate, and complicated extraction process, so as to shorten purification time, shorten extraction time, and improve extraction The effect of acquisition rate
Inactive Publication Date: 2013-02-13
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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The invention discloses a method for preparing chondroitin sulfate from sturgeon cartilage. The method comprises the following steps: (1) pretreating cartilage; (2) performing ultrasonic enzymolysis treatment: hydrolyzing the sturgeon cartilage by using trypsin, accelerating the enzymolysis process under the action of ultrasonic waves, finishing enzymolysis, deactivating enzyme, cooling and centrifuging to obtain supernate containing sturgeon chondroitin; (3) hydrolyzing and precipitating synchronously, continuously hydrolyzing the supernate in the step (2) with ethanol and NaOH solution and centrifuging to obtain white precipitate; and (4) purifying, drying and redissolving the precipitate to remove insoluble impurities, and drying to obtain a white spongy chondroitin sulfate product. The process is simple to operate and is new. Based on the steps of performing ultrasonic auxiliary enzymolysis, hydrolyzing and precipitating synchronously and purifying, the hydrolysis time can be greatly shortened, the purity and the yield of the products are increased and the production cost is reduced.
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Ultrasonic assistedChondroitin +6
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