Method for preparing chondroitin sulfate from sturgeon cartilage
A technology of chondroitin sulfate and cartilage, which is applied in the field of seafood processing, can solve the problems of unfavorable industrial production of chondroitin sulfate, low purity of chondroitin sulfate, and complicated extraction process, so as to shorten purification time, shorten extraction time, and improve extraction The effect of acquisition rate
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[0012] Example 1
[0013] The method for extracting and purifying sturgeon chondroitin sulfate provided in this embodiment includes the following steps: 1) Sample pretreatment: take the sturgeon cartilage tissue and treat it in a water bath at 80°C for 20 minutes to remove impurities such as muscle and fat on the surface. Wet ultramicro Crush to make a wet sample with a particle size of 100 mesh.
[0014] 2) Extraction of sturgeon sulphuric acid cartilage: wet cartilage sample with 0.1M phosphate buffer solution, pH 6.5 mass volume ratio of 10 (g / ml), the enzyme used for enzymatic hydrolysis is trypsin, and the added amount is per gram of sturgeon The fish cartilage sample is added to 1000U. The ultrasonic-assisted enzymatic hydrolysis conditions were 35kHz frequency, 280W power, and 50°C temperature adjustment for 50min, then the enzyme was inactivated at 95°C for 5min, and the supernatant was obtained by freezing and centrifuging at 4°C for 10min.
[0015] 3) Hydrolysis and purif...
Example Embodiment
[0016] Example 2
[0017] The method for extracting and purifying sturgeon chondroitin sulfate provided in this embodiment includes the following steps: 1) Sample pretreatment: take the sturgeon cartilage tissue and treat it in a water bath at 90°C for 10 minutes to remove impurities such as muscle and fat on the surface, dry, crush, and dry The temperature is 50°C, and it is crushed to make a dry sample with a particle size of 80 mesh.
[0018] 2) Extraction of sturgeon sulfate cartilage: dry cartilage sample with 0.1M phosphate buffer solution, pH 7.0 mass volume ratio of 10 (g / ml), the enzyme used for enzymatic hydrolysis is trypsin, and the amount added per gram of sturgeon The cartilage sample was added to 2000U. The ultrasonic-assisted enzymatic hydrolysis conditions are 35kHz frequency, 360W power, and temperature adjusted to 40°C for enzymatic hydrolysis for 20 minutes, then the enzyme is inactivated at 95°C for 5 minutes, and the supernatant is obtained by freezing and c...
Example Embodiment
[0020] Example 3
[0021] The method for extracting and purifying sturgeon chondroitin sulfate provided in this embodiment includes the following steps:
[0022] 1) Sample pretreatment: Take the sturgeon cartilage tissue and treat it in a water bath at 85°C for 20 minutes to remove impurities such as muscle and fat on the surface. The drying temperature is 48°C and the crushed particle size is 120 mesh.
[0023] 2) Sturgeon sulfate cartilage extraction: the mass-volume ratio of cartilage sample to phosphate buffer solution 0.1M, pH 7.0 is 10 (g / ml), the enzyme used for enzymatic hydrolysis is trypsin, and the added amount is per gram of sturgeon cartilage The sample is added to 2000U. The ultrasound-assisted enzymatic hydrolysis conditions were 35kHz frequency, 360W power, and temperature adjusted to 45°C for enzymatic hydrolysis for 30 minutes, then the enzyme was inactivated at 95°C for 5 minutes, and the supernatant was obtained by freezing and centrifuging at 0°C for 10 minute...
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