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Method for preparing chondroitin sulfate from sturgeon cartilage

A technology of chondroitin sulfate and cartilage, which is applied in the field of seafood processing, can solve the problems of unfavorable industrial production of chondroitin sulfate, low purity of chondroitin sulfate, and complicated extraction process, so as to shorten purification time, shorten extraction time, and improve extraction The effect of acquisition rate

Inactive Publication Date: 2013-02-13
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional extraction method of chondroitin sulfate in animal cartilage tissue is time-consuming and complicated, and the purity of chondroitin sulfate is low, which is not conducive to the industrial production of chondroitin sulfate in sturgeon cartilage tissue

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The extraction and purification method of sturgeon chondroitin sulfate provided in this example includes the following steps: 1) Sample pretreatment: take sturgeon cartilage tissue and treat it in a water bath at 80°C for 20 minutes to remove impurities such as muscle and fat on the surface. Pulverize to make a wet sample with a particle size of 100 mesh.

[0014] 2) Extraction of sulfated cartilage from sturgeon: wet cartilage samples are mixed with 0.1M phosphate buffer solution, pH 6.5 with a mass-volume ratio of 10 (g / ml), the enzyme used for enzymatic hydrolysis is trypsin, and the amount added is Add 1000U of fish cartilage samples. The ultrasonic-assisted enzymolysis conditions were frequency 35kHz, power 280W, temperature adjusted at 50°C for 50 minutes, enzyme inactivation at 95°C for 5 minutes, and refrigerated centrifugation at 4°C for 10 minutes to obtain a supernatant.

[0015] 3) Hydrolysis and purification of sturgeon cartilage sulfate: the volume of abs...

Embodiment 2

[0017] The extraction and purification method of sturgeon chondroitin sulfate provided in this example includes the following steps: 1) Sample pretreatment: take sturgeon cartilage tissue and treat it in a water bath at 90°C for 10 minutes to remove impurities such as muscle and fat on the surface, dry and pulverize, and dry The temperature is 50°C, and it is pulverized to make a dry sample with a particle size of 80 mesh.

[0018] 2) Extraction of sulfated cartilage from sturgeon: dry cartilage sample and phosphate buffer solution 0.1M, pH 7.0 mass volume ratio is 10 (g / ml), the enzyme used for enzymatic hydrolysis is trypsin, and the amount added is 1 gram of sturgeon Add 2000U of cartilage samples. The ultrasonic-assisted enzymatic hydrolysis conditions were frequency 35kHz, power 360W, temperature adjusted at 40°C for 20 minutes, enzyme inactivation at 95°C for 5 minutes, and refrigerated centrifugation at 3°C ​​for 10 minutes to obtain a supernatant.

[0019] 3) Purifica...

Embodiment 3

[0021] The method for extracting and purifying sturgeon chondroitin sulfate provided in this example comprises the following steps:

[0022] 1) Sample pre-treatment: Take sturgeon cartilage tissue and treat it in a water bath at 85°C for 20 minutes to remove impurities such as muscle and fat on the surface. The drying temperature is 48°C, and the crushed particle size is 120 mesh.

[0023] 2) Extraction of sturgeon sulfated cartilage: cartilage sample and 0.1M phosphate buffer solution, pH 7.0, the mass-to-volume ratio is 10 (g / ml), the enzyme used for enzymatic hydrolysis is trypsin, and the amount added is 1 gram of sturgeon cartilage Add 2000U of sample. The ultrasonic-assisted enzymatic hydrolysis conditions were frequency 35kHz, power 360W, temperature adjusted at 45°C for 30 minutes, enzyme inactivation at 95°C for 5 minutes, and refrigerated centrifugation at 0°C for 10 minutes to obtain the supernatant.

[0024] 3) Purification of sulfated cartilage from sturgeon: The...

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Abstract

The invention discloses a method for preparing chondroitin sulfate from sturgeon cartilage. The method comprises the following steps: (1) pretreating cartilage; (2) performing ultrasonic enzymolysis treatment: hydrolyzing the sturgeon cartilage by using trypsin, accelerating the enzymolysis process under the action of ultrasonic waves, finishing enzymolysis, deactivating enzyme, cooling and centrifuging to obtain supernate containing sturgeon chondroitin; (3) hydrolyzing and precipitating synchronously, continuously hydrolyzing the supernate in the step (2) with ethanol and NaOH solution and centrifuging to obtain white precipitate; and (4) purifying, drying and redissolving the precipitate to remove insoluble impurities, and drying to obtain a white spongy chondroitin sulfate product. The process is simple to operate and is new. Based on the steps of performing ultrasonic auxiliary enzymolysis, hydrolyzing and precipitating synchronously and purifying, the hydrolysis time can be greatly shortened, the purity and the yield of the products are increased and the production cost is reduced.

Description

technical field [0001] The invention relates to a method for preparing chondroitin sulfate by using sturgeon cartilage, belonging to the technical field of seafood processing. Background technique [0002] With the success and promotion of sturgeon artificial breeding and breeding technology, the breeding area and production of sturgeon are increasing year by year. The main purpose of sturgeon farming is to produce sturgeon roe sauce, which is known as black gold. In addition to sturgeon roe, other parts of sturgeon are also of great development value. Except for the back bone plate, the whole body of sturgeon is cartilage. Cartilage occupies a large proportion in all tissues of sturgeon. This part has obvious anti-cancer and calcium supplementation effects. This is mainly because the cartilage tissue of sturgeon contains a large amount of chondroitin sulfate. According to reports, the content of chondroitin sulfate in cartilage in the head, notochord and fin of sturgeon is...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 郝淑贤李来好杨贤庆魏涯黄卉林婉玲邓建朝岑剑伟戚勃吴燕燕石红刁石强
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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