2 results about "Tryptic hydrolysis" patented technology

The application discloses a bone-strengthening milk protein extract and a preparation method and application thereof, and belongs to the technical field of deep processing of cow milk. The bone-strengthening milk protein extract contains a bone-strengthening active peptide FYPELFR, and the preparation method specifically comprises the following steps: (1) pasteurization; (2) casein separation; (3) pepsin hydrolysis; (4) trypsin hydrolysis; (5) purification and desalination; and (6) concentration and drying. The bone-strengthening active peptide and the bone-strengthening milk protein extract containing the same can be combined with integrin alpha v beta 1 through hydrophobic interaction and hydrogen bonding, can promote the proliferation and differentiation of osteoblasts by activating specific signal pathways, can promote the proliferation of osteoblasts, can be used for the development of bone-strengthening functional food or medicine, and has important significance for the deep processing of cow milk.

This invention belongs to the field of biopharmaceuticals and relates to an α S1 - Casein polypeptides and their application in the preparation of enteritis drugs. Specifically, this invention utilizes bioinformatics technology to analyze a novel casein subtype α. S1 The trypsin hydrolysate of -casein was used to select peptides with -10lgp>30 for bulk docking prediction with claudin-15 and ZO-1 proteins. The peptide that could bind to both proteins with the lowest binding energy was found. In vivo experiments in mouse models confirmed that the peptide could significantly reduce serum IL-6 levels and had a repairing and improving effect on DSS-induced inflammatory bowel disease, making it a potential drug for the treatment of inflammatory bowel disease.