5 results about "Tryptic hydrolysis" patented technology

The invention discloses oyster pallium hexapeptide with an effect of promoting osteogenesis as well as a preparation method and application of the oyster pallium hexapeptide. The amino acid sequence of the oyster pallium hexapeptide is Phe-Phe-Glu-Pro-Lys-Phe. Fresh oyster mantle is used as a raw material, pepsin and trypsin are used for hydrolysis and centrifugation to obtain supernate, then ultrafiltration is carried out to obtain filtrate with the molecular weight being 1000 Da or below, finally freeze drying is carried out, the sequence of oyster hydrolytic peptide is obtained through mass spectrum identification, and by means of a molecular docking technology and a database prediction simulation method, the oyster hydrolytic peptide is obtained. Oyster peptide with high osteogenesis promoting capacity is screened from a plurality of peptide sequences. The oyster hexapeptide obtained by the invention has the effects of improving osteoblast proliferation and differentiation capacity and improving dexamethasone-induced zebra fish bone loss, and can be used as a beneficial dietary supplement in related products for delaying bone loss.

The invention discloses a skin repair dressing based on a crocodile scale extract and a preparation method thereof, and relates to the technical field of biological medicines and cosmetics, and the preparation method comprises the following steps: enzymatic hydrolysis cross-linking: decalcified crocodile scales are treated with lignin peroxidase under an acidic condition, hydroxypropyl-beta-cyclodextrin is synchronously added, and a collagen cross-linked structure is cracked; the pH is adjusted to be alkaline, and alkaline protease hydrolysis and trypsin hydrolysis are sequentially carried out after hydroxypropyl-beta-cyclodextrin is added; inclusion purification: adding hydroxypropyl-beta-cyclodextrin inclusion active peptide into the filtrate after ultrafiltration, and concentrating and drying to obtain collagen peptide dry powder; and dressing forming: mixing the peptide dry powder with a gel matrix, a humectant and a preservative, homogenizing and sterilizing to obtain a final product. The crocodile scale collagen is efficiently extracted and modified, so that the skin repair dressing with excellent biological activity and stability is prepared.

The application discloses a method for detecting trypsin in a hydrogel microchannel filled with glutaraldehyde cross-linked with human serum albumin. Trypsin has specific hydrolysis on HSA, and the hydrogel will expose carboxyl after hydrolysis. Therefore, when the hydrogel is hydrolyzed by trypsin, the change of the charge density of the hydrogel microchannel will be triggered, leading to the change of the ion current, so that the detection of trypsin is realized. The performance of the trypsin sensor is adjusted by adjusting experimental influencing factors (such as HSA concentration, electrolyte concentration, pH value and reaction time). Good feedback is obtained in actual sample measurement. The present study successfully performs microscale ICR experiments in the microchannel, thereby successfully constructing the trypsin sensor. The hydrogel-filled microchannel not only retains the advantages of the original nanochannel, but also solves the defects of nanochannel blockage, high operation difficulty and the like. It can also provide some references for the measurement of macromolecular targets in nanochannels / microchannels.

This invention belongs to the field of biopharmaceuticals and relates to an α S1 - Casein polypeptides and their application in the preparation of enteritis drugs. Specifically, this invention utilizes bioinformatics technology to analyze a novel casein subtype α. S1 The trypsin hydrolysate of -casein was used to select peptides with -10lgp>30 for bulk docking prediction with claudin-15 and ZO-1 proteins. The peptide that could bind to both proteins with the lowest binding energy was found. In vivo experiments in mouse models confirmed that the peptide could significantly reduce serum IL-6 levels and had a repairing and improving effect on DSS-induced inflammatory bowel disease, making it a potential drug for the treatment of inflammatory bowel disease.