Aseptic collagen liquid with biological activity and preparation method thereof

A technology of biological activity and collagen solution, which is applied in the direction of medical preparations containing active ingredients, skin care preparations, drug combinations, etc., can solve the problems of reducing collagen antigenicity, difficult to realize the supply process, unstable process, etc., and achieve shortening Purification time, avoid denaturation and inactivation, and increase the effect of contact area

Pending Publication Date: 2016-10-05
GUANGZHOU TRAUER BIOTECH
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AI Technical Summary

Problems solved by technology

Its advantage lies in maintaining the triple helical structure of collagen molecules to the greatest extent, that is, biological activity, and its disadvantage is mainly low yield
The main disadvantage of extracting collagen by the neutral salt method is that the process is unstable, which makes the molecular structure of the extracted collagen unstable.
[0005] The existing conventional enzymatic method has the advantages of fast hydrolysis reaction and

Method used

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  • Aseptic collagen liquid with biological activity and preparation method thereof

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preparation example Construction

[0033] A preparation method of a biologically active sterile collagen solution, comprising the steps of:

[0034] 1) Degrease, slice and sterilize the tendon of the animal;

[0035] 2) Pulverizing the above-mentioned sterilized tendons under ultra-low temperature conditions to obtain filamentous tendon fibers;

[0036] 3) The pulverized tendon tissue is acid-dissolved and enzymatically hydrolyzed, and then the slag is filtered to obtain the original collagen solution;

[0037] 4) The collagen stock solution is further salted out and purified to obtain a high-purity collagen solution;

[0038] 5) Fill the purified high-purity collagen solution in a clean environment of class 100,000 or above, subpackage and seal;

[0039] 6) Freeze the sealed high-purity collagen solution;

[0040] 7) Put the frozen collagen solution into a foam box or an incubator equipped with dry ice or an ultra-low temperature freezer, seal it, and sterilize it with low-temperature radiation. After thawi...

Embodiment 1

[0055] 1) Fresh healthy beef tendon, remove the fat and fascia, wash to remove impurities, freeze and slice, sterilize and save for later use

[0056] 2) Take the above-mentioned sterilized beef tendons and grind them at -80°C to -60°C to obtain filamentous tendon fibers;

[0057] 3) Add 100 times the volume of a mixture of 0.5mol / L glacial acetic acid and 400mg / L pepsin to the tendon fibers, and continue stirring for 3 hours. The ambient temperature range is 25-28°C, and the stirring speed is 50rpm;

[0058] The above mixed solution was filtered to remove slag to obtain the collagen stock solution;

[0059] 4) Add the above collagen stock solution to an equal volume of 1.4mol / L sodium chloride solution for salting out, take the precipitate, add 0.5mol / L glacial acetic acid to dissolve the precipitate, and purify it through ultrafiltration equipment, ultrafiltration system The conditions are: the pressure at the mouth end is 40psi, the return end is 10psi, and the high-purity c...

Embodiment 2

[0064] 1) Fresh healthy pork tendon, remove the fat and fascia, wash to remove impurities, freeze and slice, sterilize and save for later use

[0065] 2) Take the above-mentioned sterilized pork tendons and grind them at -150°C to -100°C to obtain filamentous tendon fibers;

[0066] 3) Add 150 times the volume of a mixture of 0.4mol / L glacial acetic acid and 300mg / L pepsin to the tendon fibers, and continue to stir for 10 hours. The ambient temperature range is 25-27°C, and the stirring speed is 10rpm;

[0067] The above-mentioned mixed solution is filtered through a 40-mesh filter screen to remove slag to obtain a collagen stock solution;

[0068] 4) Add the above collagen stock solution to an equal volume of 1.4mol / L sodium chloride solution for salting out, take the precipitate, add 0.4mol / L glacial acetic acid to dissolve the precipitate, and purify it through ultrafiltration equipment, ultrafiltration system The conditions are: the pressure at the mouth end is 10psi, the...

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Abstract

The invention discloses an aseptic collagen liquid with biological activity and a preparation method thereof. The preparation method of the aseptic collagen liquid comprises degreasing, slicing, disinfecting and crushing at a low temperature animal tendons, carrying out acid dissolution and enzymolysis, carrying out filtration to remove residues, and carrying out salting-out, purification, loading, sealing, refrigeration and low temperature irradiation sterilization to obtain the aseptic collagen liquid. The preparation method realizes low temperature irradiation, keeps collagen activity, and prevents collagen inactivation and denaturation or cross-linking reaction under irradiation so that functions are not influenced. The preparation method utilizes ultrafiltration purification to replace the traditional dialysis purification, shortens purification time, utilizes an automatic system to replace the traditional semi-automatic operation, prevents human hand pollution and guarantees product cleanliness, purity and bioactivity.

Description

technical field [0001] The invention relates to a biologically active sterile collagen solution and a preparation method thereof. Background technique [0002] Collagen is a fibrous extracellular protein twisted into a helical shape by three peptide chains, accounting for more than 30% of the protein in the whole body, and has unique physiological activities. Collagen is rich in essential amino acids such as glycine, proline, and hydroxyproline, and is the most important component of the extracellular matrix. It has strong biological activity and biological functions, and can participate in cell migration, differentiation, and proliferation. Make bone, tendon, cartilage and skin have a certain mechanical strength, and because of its weak antigenicity and good biocompatibility, it can be used in medical and health fields such as burns, trauma, corneal diseases, cosmetology, hard tissue repair, and wound hemostasis widely. [0003] Collagen is the most abundant protein in ma...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06A61K38/39A61P17/02A61K8/65A61Q19/00
Inventor 李奕恒陈淡嫦曾进朋周小洁
Owner GUANGZHOU TRAUER BIOTECH
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