An extraction apparatus for the separation of natural products

Abstract

The application discloses an extraction device for natural product separation, which comprises an extraction tank, a membrane separation assembly, a stirring motor, a temperature control assembly and a controller. The temperature control assembly adopts an up-down partitioned cold-hot temperature control design, and a heat insulation partition fence is arranged between the cooling pipe and the heating pipe. In combination with an anchor type gentle stirring, denaturation of active ingredients is avoided. The membrane separation assembly realizes three-stage gradient separation through a primary ultrafiltration membrane, a medium-level nanofiltration membrane and a high-level affinity membrane. The high-level affinity membrane is loaded with replaceable biological ligands to accurately enrich target components. A structure for real-time detection of concentration and activity of an extraction agent is matched, and the whole process is automatically regulated by electric control valves and the controller. The application can efficiently separate natural active substances of targeted membrane protein targets and drug resistance related targets, completely retains the activity of components, has high separation purity, high automation degree, and recyclable extraction agent, is suitable for laboratory research and development and small-scale industrial production, and meets the extraction requirements in the fields of biological experiments, agriculture and biological medicines.

Description

Technical Field

[0001] This invention relates to the field of natural product processing equipment technology, and more particularly to an extraction apparatus for separating and extracting natural products, including membrane protein targets, active pharmaceutical ingredients, and drug resistance-related active substances. Background Technology

[0002] Natural products are important raw material sources for drug development and biological research. Among them, membrane proteins, active ingredients that bind to drug targets, and drug resistance-related active substances are research hotspots in the biomedical field. Membrane proteins, as core components of biological membranes, participate in various physiological processes such as substance transport and signal transduction, and are important drug targets. However, their fragile structure makes them susceptible to denaturation and inactivation due to factors such as temperature, pressure, and shear force, making it difficult for traditional extraction devices to achieve efficient separation under mild conditions. Active ingredients that bind to drug targets are usually present in low concentrations in natural products and are easily mixed with other impurities. The separation purity and extraction rate of traditional extraction devices are insufficient to meet research requirements. Furthermore, with the increasing prominence of bacterial drug resistance, screening for drug resistance-inhibiting active substances from natural products has become a research focus. However, traditional devices lack targeted separation structures, making it impossible to achieve precise extraction of these active substances.

[0003] Existing natural product extraction devices have the following main drawbacks: First, they lack pretreatment and temperature and pressure control structures, making it impossible to preserve the activity of membrane proteins and easily leading to denaturation and inactivation of membrane proteins during extraction; Second, they cannot achieve high-purity separation of active ingredients targeting drugs and are difficult to adapt to the extraction requirements of drug resistance-related active substances; Third, the operation process is complex and the degree of automation is low, making it difficult to meet the diverse needs of laboratory research and development and small-scale industrial production.

[0004] Therefore, developing an extraction device that can efficiently separate membrane proteins, drug targets, and drug resistance-related natural active substances, while retaining the activity of the target components, improving separation purity, and being easy to operate, has become an urgent technical problem to be solved. Summary of the Invention

[0005] This invention aims to address at least one of the technical problems existing in the prior art. To this end, this invention provides an extraction apparatus for the separation of natural products, designed to solve the problem that existing devices cannot accurately extract membrane protein targets and related active substances simultaneously, while improving extraction efficiency and separation purity, reducing production costs, achieving automated operation, and meeting the research and production needs of the biopharmaceutical field.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: an extraction device for separating natural products, comprising: an extraction tank, the bottom of which is provided with a discharge end and a first electrically controlled valve; a tank cover, disposed on the top of the extraction tank, the tank cover being provided with a feed end, a filling port and a pressure relief port, the feed end, the filling port and the pressure relief port being respectively provided with a second electrically controlled valve, a third electrically controlled valve and a fourth electrically controlled valve; a stirring mechanism, including a stirring motor disposed on the tank cover and a stirring rod connected to the output end of the stirring motor, the stirring rod extending into the interior of the extraction tank; an outer shell, disposed outside the extraction tank; and a temperature control component, disposed between the extraction tank and the outer shell, including a cooling pipe and a heating pipe, the cooling pipe... A cooling tube spiral is located in the upper region of the extraction tank, and a heating tube spiral is located in the lower region of the extraction tank. A partition plate made of heat-insulating material is provided between the cooling tube and the heating tube. A membrane separation assembly, connected to the outlet end of the extraction tank, includes a primary ultrafiltration membrane, a secondary nanofiltration membrane, and a higher affinity membrane connected in series. The primary ultrafiltration membrane is used to retain large molecular impurities in the natural product, the secondary nanofiltration membrane is used to separate small molecular impurities from the target component, and the higher affinity membrane is used to specifically bind membrane proteins or drug target active ingredients to improve separation purity. A controller is electrically connected to the stirring motor, temperature control assembly, and each electrically controlled valve to control the automated operation of the extraction process.

[0007] Preferably, the device also includes a pulverizer disposed above the can lid, wherein the discharge port of the pulverizer is connected to the feed end of the can lid via a feed pipe, and a fifth electrically controlled valve electrically connected to the controller is provided at the discharge port.

[0008] Preferably, the stirring rod includes a stirring shaft and a stirring rod. The stirring shaft passes through the tank cover and extends into the interior of the extraction tank, and a stirring rod is provided at its bottom end. The stirring rod is an anchor-type stirring paddle.

[0009] Preferably, the side wall of the extraction vessel is provided with an observation window made of transparent material, and the outer shell is provided with an observation port corresponding to the position of the observation window.

[0010] Preferably, the primary ultrafiltration membrane has a pore size of 0.1-0.5 μm, the intermediate nanofiltration membrane has a pore size of 0.01-0.1 μm, and the surface of the advanced affinity membrane is modified with a biological ligand, which is a biotin, antibody, or enzyme molecule and can be replaced according to the separation target. The advanced affinity membrane is made of polyethersulfone, which has good biocompatibility and stability, and avoids damage to the spatial structure of membrane proteins.

[0011] Preferably, the system also includes a collection component, which comprises a staged collection tank and a detection unit. The staged collection tank is connected to the outlet of the membrane separation component. The detection unit comprises a concentration detector and an activity detector. The concentration detector is used to detect the concentration of the target component in the collected liquid, and the activity detector is used to detect the activity of the membrane protein or the bioactivity of the drug target component, thereby monitoring the extraction effect in real time.

[0012] Preferably, the system also includes an extractant storage tank and a circulation pump, wherein the outlet of the extractant storage tank is connected to the filling port of the extractant tank via the circulation pump.

[0013] Preferably, the system also includes an extractant recovery unit, which comprises a condenser and a molecular sieve adsorption tower. The inlet of the condenser is connected to the waste liquid outlet of the membrane separation component, the outlet of the condenser is connected to the inlet of the molecular sieve adsorption tower, and the outlet of the molecular sieve adsorption tower is connected to the inlet of the extractant storage tank.

[0014] Preferably, the extraction tank is equipped with a temperature sensor, a pressure sensor, and a first liquid level sensor electrically connected to the controller, and the extractant storage tank is equipped with a second liquid level sensor electrically connected to the controller. When the extractant level is lower than a set value, the controller issues an alarm.

[0015] Preferably, the inner wall of the extraction tank is polished and coated with an anti-corrosion coating.

[0016] The present invention has the following advantages due to the adoption of the above technical solutions: 1. This invention utilizes the synergistic effect of a three-stage membrane separation component (primary ultrafiltration membrane, intermediate nanofiltration membrane, and advanced affinity membrane) combined with replaceable biological ligand modification to achieve precise separation of membrane protein targets, active pharmaceutical ingredients, and drug resistance-related active substances from natural products, solving the problem of insufficient separation targeting in traditional devices. Simultaneously, through precise temperature and pressure control via upper and lower partition temperature control components, the bioactivity of the target natural products bound to the membrane protein targets is effectively preserved, avoiding denaturation and inactivation of the target components.

[0017] 2. This invention pre-treats the raw materials using a pulverizer, increasing the contact area between the raw materials and the extractant and removing impurities. The anchor-type stirring paddle achieves gentle and uniform mixing, avoiding damage to active ingredients by high shear forces and accelerating the dissolution rate of the target components. The three-stage membrane separation structure achieves gradient separation, and the specific binding of the high-affinity membrane further improves the separation purity. The extraction rate and separation purity of membrane proteins and drug target active ingredients are significantly improved, meeting the needs of biomedical research and development.

[0018] 3. This invention achieves the recycling of extractant by setting up an extractant recovery unit, thereby reducing the waste of extractant and environmental pollution; the device adopts PLC intelligent control, which has a high degree of automation, reduces the labor intensity of operators, reduces human operation errors, improves production efficiency, and reduces production costs.

[0019] 4. The modules of this invention are detachable, which facilitates cleaning, maintenance and replacement (such as replacement of membrane separation components); the intelligent control panel enables one-click setting and real-time monitoring of various parameters, making it easy to operate; it is suitable for laboratory research and development and small-scale industrial production, with a wide range of applications and good practicality and promotion value.

[0020] 5. This invention enables real-time monitoring by multiple sensors, parameter adjustment via a touch screen, and intuitive observation via an observation window, making the entire extraction process controllable, visible, and traceable, thereby improving production stability and product qualification rate.

[0021] 6. This invention can efficiently separate natural active substances targeting membrane protein targets and drug resistance-related targets, fully preserving the activity of the components. It has high separation purity, high degree of automation, and recyclable extraction solvent, making it suitable for laboratory research and development as well as small-scale industrial production, and meeting the extraction needs of biological experiments, agriculture, and biomedicine. Attached Figure Description

[0022] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the invention. Throughout the drawings, the same reference numerals denote the same parts. In the drawings: Figure 1 This is a front view of the extraction device in an embodiment of the present invention; Figure 2 This is a side view of the extraction device in an embodiment of the present invention; Figure 3 This is a bottom view of the extraction device in an embodiment of the present invention; Figure 4 This is a schematic diagram of the extraction device after removing the outer shell in an embodiment of the present invention; Figure 5 This is a schematic diagram of the structure of the stirring rod in an embodiment of the present invention.

[0023] The labels for the attached figures are as follows: 1-Extraction tank; 2-Outer shell; 3-Tank lid; 4-Supporting foot; 5-Pulverizer; 6-Stirring motor; 7-Control box; 8-Touch screen; 9-Multi-function button; 10-Feed end; 11-Feed pipe; 12-Adding port; 13-Pressure relief port; 14-Pressure gauge; 15-Observation window; 16-Observation port; 17-First electrically controlled valve; 18-Second electrically controlled valve; 19-Third electrically controlled valve; 20-Discharge end; 21-Fourth electrically controlled valve; 22-Cooling pipe; 23-Heating pipe; 24-Partition plate; 25-Stirring shaft; 26-Stirring rod. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the present invention clearer, specific embodiments of the present invention will be further described below with reference to the accompanying drawings. Although exemplary embodiments of the present invention are shown in the drawings, it should be understood that the present invention can be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0025] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0026] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0027] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0028] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "over," and "on top" of the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0029] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0030] The extraction apparatus for separating natural products provided by this invention includes an extraction tank, a membrane separation component, a stirring motor, a temperature control component, and a controller. The temperature control component adopts an upper and lower partitioned cold and hot temperature control design, with a heat-insulating partition between the cooling and heating pipes, combined with anchor-type gentle stirring to prevent denaturation of active ingredients. The membrane separation component achieves three-stage gradient separation through a primary ultrafiltration membrane, a secondary nanofiltration membrane, and a higher affinity membrane. The higher affinity membrane is equipped with replaceable biological ligands for precise enrichment of target components. It is equipped with a structure for recycling the extraction solvent and real-time monitoring of concentration and activity. The entire process is automatically controlled by electronically controlled valves and a controller. This invention can efficiently separate natural active substances targeting membrane protein targets and drug resistance-related targets, fully preserving the activity of the components. It features high separation purity, a high degree of automation, and recyclable extraction solvent, making it suitable for laboratory research and small-scale industrial production, meeting the extraction needs of biological experiments, agriculture, and biomedicine.

[0031] The extraction apparatus for separating natural products provided in the embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0032] Please see Figures 1 to 5This invention provides an extraction device for separating natural products, comprising an extraction tank 1 and a tank cover 3 disposed on its top; the inner wall of the extraction tank 1 is polished and coated with an anti-corrosion coating; a stirring motor 6 is disposed in the middle of the top of the tank cover 3, and a stirring rod is disposed at the output end of the stirring motor 6, the stirring rod penetrating the tank cover 3 and extending into the interior of the extraction tank 1; the tank cover 3 is also provided with a feed end 10, a filling port 12, and a pressure relief port 13, and the feed end 10, the filling port 12, and the pressure relief port 13 are respectively provided with a first electrically controlled valve 17, a second electrically controlled valve 18, and a third electrically controlled valve 19; an outer shell 2 is disposed on the side of the extraction tank 1, and a temperature control component is disposed between the outer shell 2 and the extraction tank 1; the bottom of the extraction tank 1 is provided with... The extraction tank 1 is equipped with a discharge end 20 and a fourth electrically controlled valve 21. The temperature control assembly includes a cooling pipe 22 and a heating pipe 23. The heating pipe 23 is spirally arranged between the extraction tank 1 and the outer shell 2 near the bottom of the extraction tank 1. The cooling pipe 22 is spirally arranged between the extraction tank 1 and the outer shell 2 near the top of the extraction tank 1. A partition plate 24 made of heat-insulating material is provided between the cooling pipe 22 and the heating pipe 23. The extraction tank 1 is equipped with a temperature sensor, a pressure sensor and a first liquid level sensor. A pressure gauge is provided at the pressure relief port 13. A control box 7 is provided on the surface of the outer shell 2. A PLC controller is provided inside the control box 7. A touch screen display 8 and a multi-function button 9 are provided on the surface of the control box 7.

[0033] By adopting the above technical solutions, the polishing treatment of the inner wall of the extraction tank 1 can reduce material adhesion residue, and the anti-corrosion coating is suitable for the acid and alkaline conditions of natural product extraction, extending the service life of the equipment; the stirring motor 6 drives the stirring rod to achieve full mixing of materials and extractant, improving mass transfer extraction efficiency; the electrical control valves at each port, together with the PLC controller, realize the automated control of feeding, adding extractant, depressurization, and discharging, simplifying manual operation; the temperature control component adopts an upper and lower partition temperature control design, and the cooling pipe 22 and heating pipe 23 are separated by a heat-insulating partition plate 24 to achieve precise temperature control inside the tank and prevent sudden temperature changes from damaging the activity of membrane proteins; temperature, pressure, and liquid level sensors collect the operating data inside the tank in real time, and the pressure gauge displays the depressurization pressure intuitively. Together with the PLC controller and touch screen 8, the extraction process can be visualized and automatically controlled, ensuring a mild and stable extraction environment.

[0034] Further, please refer to Figure 2A crusher 5 is installed above the feed end 10, and the crusher 5 has a feed inlet and a discharge outlet; the discharge outlet of the crusher 5 is equipped with a fifth electrically controlled valve; the feed end 10 is equipped with a feed pipe 11 connected to the discharge outlet of the crusher 5. By adopting the above technical solution, the device integrates crushing pretreatment function, eliminating the need for additional crushing equipment and simplifying the natural product processing flow; the crusher 5 can crush the raw materials to a suitable particle size, increasing the contact area between the raw materials and the extractant, and improving the extraction efficiency; the fifth electrically controlled valve is controlled by a PLC controller to realize automatic feeding of the crushed material, improving the integration and automation level of the device.

[0035] Further, please refer to Figure 2 , Figure 5 The stirring rod includes a stirring shaft 25 and a stirring rod 26. The stirring shaft 25 passes through the tank cover 3 and extends into the interior of the extraction tank 1, with the stirring rod 26 located at its bottom end. The stirring rod 26 is an anchor-type stirring paddle. By adopting the above technical solution, the anchor-type stirring paddle rotates in close contact with the inner wall of the extraction tank 1, eliminating dead zones in the tank and ensuring uniform mixing of materials. At the same time, the anchor-type paddle provides gentle shear force, avoiding denaturation and inactivation of active ingredients such as membrane proteins and drug targets due to high shear force, thus balancing mixing effect and retention of component activity.

[0036] Further, please refer to Figure 1 The extraction tank 1 has a transparent observation window 15 on its side, and the outer shell 2 has an observation port 16 corresponding to the observation window 15. By adopting the above technical solution, the observation window 15 and the observation port 16 work together to facilitate the operator to observe the mixing state of the materials in the extraction tank 1, the liquid level, and the extraction reaction process in real time, so as to detect abnormal conditions in a timely manner and ensure the stable operation of the extraction process.

[0037] Furthermore, it also includes a membrane separation component (not shown in the figure), which is detachably connected to the outlet end 20 of the extraction tank 1. The membrane separation component includes a primary ultrafiltration membrane, a secondary nanofiltration membrane, and a higher affinity membrane connected in series. The primary ultrafiltration membrane has a pore size of 0.1-0.5 μm and is used to retain macromolecular impurities in natural products. The secondary nanofiltration membrane has a pore size of 0.01-0.1 μm and is used to separate small molecule impurities from the target component. The surface of the higher affinity membrane is modified with biological ligands for specific binding of membrane proteins or drug target active ingredients, thereby improving separation purity. By adopting the above technical solution, the three-stage membrane components are connected in series to form a gradient separation system. The primary ultrafiltration membrane retains macromolecular impurities such as plant fibers and protein aggregates, the secondary nanofiltration membrane separates small molecule impurities such as sugars and inorganic salts, and the higher affinity membrane specifically adsorbs the target active ingredient through biological ligands, removing impurities and enriching the target product step by step, thereby significantly improving separation purity. The detachable design facilitates the cleaning, replacement, and maintenance of the membrane components and adapts to the separation needs of different natural products.

[0038] Furthermore, the biological ligands of the high-affinity membrane can be biotin, antibodies, or enzyme molecules, and can be changed according to the separation target. The high-affinity membrane is made of polyethersulfone, which has good biocompatibility and stability, avoiding damage to the spatial structure of membrane proteins. By adopting the above technical solution, the biological ligands can be flexibly changed according to the separation requirements of membrane protein targets and related active substances, improving the adaptability of the device; the polyethersulfone material has good chemical stability and excellent biocompatibility, and will not react with the target active ingredients, thus preserving the spatial structure and biological activity of membrane proteins to the greatest extent.

[0039] Furthermore, it also includes a collection component (not shown in the figure), which comprises a fractionation collection tank and a detection unit. The fractionation collection tank is connected to the outlet of the membrane separation component. The detection unit includes a concentration detector and an activity detector. The concentration detector is used to detect the concentration of the target component in the collected solution, and the activity detector is used to detect the bioactivity of the component bound to the membrane protein target, thereby monitoring the extraction effect in real time. By adopting the above technical solution, the fractionation collection tank achieves the classified collection of extracts of different purities, avoiding component mixing; the concentration detector and activity detector monitor the concentration and bioactivity of the extract in real time, transmitting the data to the PLC controller to achieve real-time monitoring and quality control of the extraction effect, ensuring that the extracted components meet the standards.

[0040] Furthermore, it also includes an extractant storage tank (not shown in the figure), the outlet of which is connected to the filling port 12 of the extraction tank 1 via a circulation pump. By adopting the above technical solution, the extractant storage tank can store a sufficient amount of extractant, the circulation pump realizes quantitative and stable addition of extractant, and with the PLC automatic control, the amount of extractant added can be precisely controlled, maintaining the stability of the extraction system and improving extraction efficiency and repeatability.

[0041] Furthermore, the system also includes an extractant recovery unit (not shown in the figure). This unit comprises a condenser and a molecular sieve adsorption tower. The condenser's inlet is connected to the waste liquid outlet of the membrane separation component, and the condenser's outlet 20 is connected to the inlet of the molecular sieve adsorption tower. The molecular sieve adsorption tower's outlet is connected to the inlet of the extractant storage tank. By adopting the above technical solution, the extractant recovery unit can recover the extractant from the separation waste liquid. The condenser condenses the gaseous extractant into a liquid state, and the molecular sieve adsorption tower removes moisture and trace impurities from the extractant. The recovered extractant is returned to the storage tank for recycling, reducing extractant consumption and production costs, minimizing waste liquid discharge, and achieving green and environmentally friendly production.

[0042] Furthermore, the circulating pump is a peristaltic pump, and the extractant storage tank is equipped with a second liquid level sensor, which is electrically connected to the PLC controller. When the extractant level is lower than the set value, the PLC controller issues an alarm. By adopting the above technical solution, the peristaltic pump has no shearing force and good sealing performance, avoiding extractant contamination and damage to the target components, and is suitable for the extraction requirements of active ingredients; the second liquid level sensor monitors the extractant level in real time, and the PLC controller automatically alarms when the level is insufficient, reminding the operator to add extractant in time, ensuring the continuous and stable operation of the device.

[0043] The overall structure and working process of the present invention will be fully described below with reference to the accompanying drawings.

[0044] like Figures 1 to 5 As shown, the extraction device for separating natural products provided by the present invention includes an extraction tank 1, an outer shell 2, a tank cover 3, support feet 4, a crusher 5, a stirring motor 6, a control box 7, a touch screen display 8, a multi-function button 9, a feed end 10, a feed pipe 11, a filling port 12, a pressure relief port 13, a pressure gauge 14, an observation window 15, an observation port 16, a first electrically controlled valve 17, a second electrically controlled valve 18, a third electrically controlled valve 19, a discharge end 20, a fourth electrically controlled valve 21, a cooling pipe 22, a heating pipe 23, a partition plate 24, a stirring shaft 25, and a stirring rod 26. It is also equipped with a membrane separation component, a collection component, an extractant storage tank, and an extractant recovery unit.

[0045] The bottom of the extraction tank 1 is evenly provided with support feet 4 to ensure that the device is placed stably; the outer shell 2 covers the outside of the extraction tank 1 and has both protection and heat preservation functions; the tank lid 3 is detachably and sealed to the extraction tank 1 to prevent material leakage and external pollution, and to ensure a closed extraction environment.

[0046] The crusher 5 is fixedly installed above the tank cover 3. Natural product raw materials are fed into the crusher through the feed port. After crushing, the PLC controller controls the opening of the electrical control valve 5. The material enters the extraction tank 1 through the discharge port, feed pipe 11, and feed end 10. The stirring motor 6 outputs power to drive the stirring shaft 25 to rotate, which drives the anchor stirring rod 26 to gently stir, so that the material and the extractant are fully mixed.

[0047] In the temperature control assembly, the cooling pipe 22 is spirally wound around the upper part of the extraction tank 1, and the heating pipe 23 is spirally wound around the lower part of the extraction tank 1. The partition plate 24 completely separates the hot and cold areas to avoid temperature crosstalk. The temperature sensor collects the temperature data inside the tank in real time and transmits it to the PLC controller to automatically adjust the start and stop of the cooling pipe and the heating pipe to achieve precise temperature control. The pressure sensor monitors the pressure inside the tank. When the pressure is over-pressurized, the third electrically controlled valve 19 is automatically opened to safely release the pressure through the pressure relief port 13. The pressure gauge 14 displays the pressure value of the pressure relief port in real time for easy and intuitive monitoring.

[0048] After extraction, the PLC controller opens the fourth electrically controlled valve 21, and the extract flows from the outlet 20 into the membrane separation component, passing sequentially through the primary ultrafiltration membrane, the intermediate nanofiltration membrane, and the advanced affinity membrane to complete gradient separation. The separated extract enters the staged collection tank of the collection component, where the concentration detector monitors the concentration of the target component in real time, and the activity detector monitors the biological activity. The detection data is simultaneously uploaded to the touch screen 8. The waste liquid generated during separation enters the extractant recovery unit, where it is condensed by a condenser and dehydrated and impurities removed by a molecular sieve adsorption tower. The purified extractant is then returned to the extractant storage tank for recycling.

[0049] The control box 7 integrates a PLC controller, the touch screen 8 is used for setting process parameters and displaying real-time operating conditions, and the multi-function button 9 supports manual mode operation, which can realize free switching between fully automatic control and manual intervention.

[0050] The working principle of this extraction device is as follows: Before use, process parameters such as extraction temperature, stirring speed, extraction time, membrane separation parameters, and alarm thresholds are set via the touch screen 8. The PLC controller completes the device initialization self-check. Natural product raw materials are fed into the pulverizer 5, which automatically starts pulverizing. The PLC controller controls the fifth electrically controlled valve to open according to the preset amount. The pulverized material enters the extraction tank 1 through the feed pipe 11 and feed end 10. After the first liquid level sensor detects that the material level reaches the target, the fifth electrically controlled valve is closed. The PLC controller opens the second electrically controlled valve 18, and the peristaltic pump injects the extractant from the extractant storage tank into the extraction tank 1 quantitatively through the injection port 12. Injection stops after the liquid level reaches the target. The stirring motor 6 starts, and the anchor-type stirring rod 26 gently stirs with low shear force, ensuring thorough mixing of the material and extractant. Temperature sensors collect the temperature inside the tank in real time, and PLC controllers regulate the heating tube 23 to raise the temperature and the cooling tube 22 to lower the temperature. Together with the partition plate 24, precise temperature control is achieved in the upper and lower zones to maintain a stable extraction temperature. Pressure sensors monitor the pressure inside the tank in real time. When the pressure exceeds the limit, the pressure relief port 13 is automatically opened to release the pressure and ensure the safety inside the tank. Operators can observe the extraction status in real time through the observation window 15.

[0051] After extraction, the fourth electrically controlled valve 21 opens, and the extract enters the membrane separation module. The primary ultrafiltration membrane retains large molecular impurities, the intermediate nanofiltration membrane separates small molecular impurities, and the advanced affinity membrane specifically binds to the target active ingredient, achieving high-purity separation. The extract flows into a fractionation collection tank, where concentration and activity detectors monitor in real time. Qualified extract is collected and stored in stages. The separation waste liquid enters the extractant recovery unit, where a condenser condenses and liquefies the extractant, and a molecular sieve adsorption tower removes moisture and impurities. The recovered extractant is returned to the extractant storage tank. A second level sensor monitors the extractant level in real time; if it falls below the set value, the PLC controller automatically alarms, reminding the user to add extractant. The entire extraction and separation process is fully automated by the PLC controller, with real-time parameter display and automatic alarm for abnormalities, achieving gentle, efficient, and high-purity separation and extraction of membrane protein targets and their bound natural product ligands or drug resistance-related active substances from natural products.

[0052] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and not to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. These modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application, and they should all be covered within the scope of the claims and specification of this application. In particular, as long as there is no structural conflict, the various technical features mentioned in the embodiments can be combined in any way. This application is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. An extraction apparatus for separating natural products, characterized in that, include: The extraction tank has a discharge end at the bottom and a first electrically controlled valve. A can lid is provided on the top of the extraction tank. The can lid is provided with a feed end, a filling port and a pressure relief port. The feed end, the filling port and the pressure relief port are respectively provided with a second electrically controlled valve, a third electrically controlled valve and a fourth electrically controlled valve. The stirring mechanism includes a stirring motor mounted on the tank lid and a stirring rod connected to the output end of the stirring motor, the stirring rod extending into the interior of the extraction tank; The outer casing is disposed outside the extraction tank; A temperature control component is disposed between the extraction tank and the outer shell, including a cooling pipe and a heating pipe. The cooling pipe is spirally disposed in the upper region of the extraction tank, and the heating pipe is spirally disposed in the lower region of the extraction tank. A partition plate made of heat-insulating material is disposed between the cooling pipe and the heating pipe. The membrane separation assembly, connected to the outlet end of the extraction tank, includes a primary ultrafiltration membrane, a secondary nanofiltration membrane, and an advanced affinity membrane connected in series. The primary ultrafiltration membrane is used to retain macromolecular impurities in the natural product, the secondary nanofiltration membrane is used to separate small molecule impurities from the target component, and the advanced affinity membrane is used to specifically bind membrane proteins or drug target active components to improve separation purity. as well as The controller is electrically connected to the stirring motor, temperature control component and each electrically controlled valve, and is used to control the automated operation of the extraction process.

2. The extraction apparatus for separating natural products according to claim 1, characterized in that, It also includes a crusher installed above the can lid, the discharge port of the crusher being connected to the feed end of the can lid via a feed pipe, and a fifth electrically controlled valve electrically connected to the controller being installed at the discharge port.

3. The extraction apparatus for separating natural products according to claim 1, characterized in that, The stirring rod includes a stirring shaft and a stirring rod. The stirring shaft passes through the tank cover and extends into the interior of the extraction tank, and a stirring rod is provided at its bottom end. The stirring rod is an anchor-type stirring paddle.

4. The extraction apparatus for separating natural products according to claim 1, characterized in that, The side wall of the extraction tank is provided with an observation window made of transparent material, and the outer shell is provided with an observation port corresponding to the position of the observation window.

5. The extraction apparatus for separating natural products according to claim 1, characterized in that, The primary ultrafiltration membrane has a pore size of 0.1-0.5 μm, the intermediate nanofiltration membrane has a pore size of 0.01-0.1 μm, and the surface of the advanced affinity membrane is modified with biological ligands, which are biotin, antibodies, or enzyme molecules and can be replaced according to the separation target. The advanced affinity membrane is made of polyethersulfone, which has good biocompatibility and stability and avoids damage to the spatial structure of membrane proteins.

6. The extraction apparatus for separating natural products according to claim 1, characterized in that, It also includes a collection component, which comprises a staged collection tank and a detection unit. The staged collection tank is connected to the outlet of the membrane separation component. The detection unit includes a concentration detector and an activity detector. The concentration detector is used to detect the concentration of the target component in the collected liquid, and the activity detector is used to detect the activity of the membrane protein or the bioactivity of the drug target component, thereby monitoring the extraction effect in real time.

7. The extraction apparatus for separating natural products according to claim 1, characterized in that, It also includes an extractant storage tank and a circulation pump, wherein the outlet of the extractant storage tank is connected to the filling port of the extractant tank through the circulation pump.

8. The extraction apparatus for separating natural products according to claim 7, characterized in that, It also includes an extractant recovery unit, which includes a condenser and a molecular sieve adsorption tower. The inlet of the condenser is connected to the waste liquid outlet of the membrane separation component, the outlet of the condenser is connected to the inlet of the molecular sieve adsorption tower, and the outlet of the molecular sieve adsorption tower is connected to the inlet of the extractant storage tank.

9. The extraction apparatus for separating natural products according to claim 8, characterized in that, The extraction tank is equipped with a temperature sensor, a pressure sensor, and a first liquid level sensor that are electrically connected to the controller. The extractant storage tank is equipped with a second liquid level sensor that is electrically connected to the controller. When the extractant level is lower than a set value, the controller issues an alarm.

10. The extraction apparatus for separating natural products according to any one of claims 1 to 9, characterized in that, The inner wall of the extraction tank is polished and coated with an anti-corrosion coating.

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