32results about How to "Prevent denaturation and inactivation" patented technology

The invention discloses a novel preparation method of solid lipid nanoparticles (SLN) (including nanostructured lipid carriers NLC), solving the unstable problem of easiness in aggregation, agglomeration, and the like of the solid lipid nanoparticles (including NLC). The preparation method comprises the steps of: a, dissolving lipid matters and lipotrophic matters (including medicines) in an organic solvent (such as tertiary butanol) capable of being mixed and dissolved with water to form an oil phase (O), or solubilizing hydrophilic matters (including medicines) in an organic solvent (O) capable of being mixed and dissolved with water by using a surfactant, wherein the lipid matters and the lipotrophic matters are used for forming the solid lipid nanoparticles (including NLC); b, dissolving water-soluble matters in water to form a water phase (W); c, injecting the oil phase (O) into the water phase (W) under stirring condition according to a proper volume proportion to obtain a solid nanoparticle dispersing solution; d, freezing and drying the obtained dispersing solution to remove the solvent to obtain a freeze-dried product; and e, hydrating the obtained freeze-dried product to obtain the solid lipid nanoparticles (including NLC). The preparation method is simple in procedures and easy to implement.

The invention discloses an aseptic collagen liquid with biological activity and a preparation method thereof. The preparation method of the aseptic collagen liquid comprises degreasing, slicing, disinfecting and crushing at a low temperature animal tendons, carrying out acid dissolution and enzymolysis, carrying out filtration to remove residues, and carrying out salting-out, purification, loading, sealing, refrigeration and low temperature irradiation sterilization to obtain the aseptic collagen liquid. The preparation method realizes low temperature irradiation, keeps collagen activity, and prevents collagen inactivation and denaturation or cross-linking reaction under irradiation so that functions are not influenced. The preparation method utilizes ultrafiltration purification to replace the traditional dialysis purification, shortens purification time, utilizes an automatic system to replace the traditional semi-automatic operation, prevents human hand pollution and guarantees product cleanliness, purity and bioactivity.

The invention discloses a bacterial strain for preparing highly effective capsula glucoprotein immuno-modulatory agent, its selection and use thereof, which relates to a strain of K. peneumoniae and its screen selecting method, and the method for preparing capsula glucoprotein by using the strain, wherein the K. peneumoniae capsula glucoprotein immuno-modulatory agent is prepared through the steps of strain screening, culture, cracking, crude extracting and purification, thus overcoming the problem of drug tolerance for antibiotics when used for treating infection.

The invention discloses a large scale human urine protein collection method. The method comprises the following steps: placing absorption agents, which are used for absorbing urine proteins and have been packaged, in the places where urine will pass through to make the absorption agent be able to absorb the urine proteins in the urine; collecting the absorption agents which have absorbed the urine proteins; subjecting the collected absorption agents to a pretreatment; refrigerating and storing the pre-treated absorption agents; and transporting the absorption agents to a processing factory in batches to carry out urine protein desorption process to produce corresponding urine protein raw materials or urine protein products. The large scale human urine protein collection method is suitable for the mass industrial urine protein production, improves the processing amount of urine protein, reduces the transportation cost of absorption agents and the production cost of urine proteins, furthermore, the absorption agents containing urine proteins are subjected to a pre-treatment before storage, thus the urine protein activity is preserved, the urine protein degradation is reduced, the microorganism generation is reduced, so that the urine protein product quality is improved.

The invention relates to a method for collecting urine protein in a large scale from human urine. The method is especially suitable for large-scale use, and comprises the following steps: manufacturing a urine filtering device by use of an anion exchanger, and placing the urine filtering device in a urinal funnel or a urinal channel for carrying the urine flowing through the filtering device, wherein the shape and size of the urine filtering device are adaptive to those of the urinal funnel or the urinal channel; and recycling the urine filtering device at fixed time, desorbing and extracting the anion exchanger in concentration, extracting the component containing the urine protein, and performing the regeneration treatment on the anion exchanger so that the anion exchanger can be further used. The urine protein with various indexes achieving the pharmacopoeia requirements can be prepared through the subsequent purification treatment, and the urine protein can be used as the substitute goods of the human plasma source protein in multiple fields. The more important is that the corresponding pretreatment is performed before performing the treatment on the adsorbent absorbing the urine protein, so that the activity of the urine protein is guaranteed, the degradation of the urine protein and the propagation of microorganisms are reduced, and the quality of the urine protein product is improved.

The invention discloses a preparation method for human antithrombin III (AT-III). The preparation method comprises the following steps: (1) precipitation and dissolution of a blood plasma fraction IV; (2) polyethylene glycol (PEG) precipitation and impure protein removing; (3) DEAE Sephadex A-50 gel adsorption; (4) S/D virus inactivation; (5) heparin affinity column chromatography; (6) ultrafiltration and concentration; (7) addition of a stabilizer and regulation; (8) nanofilm virus-removing filtration; (9) sterilizing filtration; (10) freeze-drying; (11) dry and fever virus inactivation. According to the preparation method, blood coagulation factors, which remain in the raw materials and are depended by vitamin K, are removed, so as to greatly lower the possibility of protein activation in the production process, and improve the product qualification ratio; PEG is adopted for the impure protein removing, so as to reduce the load of a chromatographic column; a liquid obtained from lyophilized powder re-dissolution is clear, transparent, and free of protein precipitation and opalescence; through adoption of a three-step virus inactivation method, the safety of human AT-III for clinical use can be greatly improved.

The invention provides a method for separating and purifying human serum albumin from a Cohn component V supernatant, which comprises the following steps of: loading a supernatant of the Cohn component V into a chromatographic column containing an anion exchange chromatographic medium, eluting the chromatographic column with eluent, collecting the eluent to obtain human serum albumin; according tothe method, a novel idea of separating and purifying albumin by using the low-temperature ethanol precipitation component V supernatant as a raw material is put forward for the first time, ethanol isnot removed by adopting an ultrafiltration process, so that the using amount of a buffer solution is greatly reduced, the operation time is reduced, the method avoids the problem of denaturation andinactivation of the protein in a higher ethanol solution for a long time, and has the advantages of simple operation, low cost, short process time consumption, high protein activity yield and the like. The method can further recover the albumin in the supernatant of the component V, effectively improve the utilization rate of the plasma and reduce the cost, and has important value for practical application.

The invention discloses a preparation method of a liquid woundplast based on stem cell culture supernatant. The liquid woundplast comprises a film-forming solution, an active substance and a solvent; the film-forming solution comprises 2-10 parts of chitosan hydrochloride, 0.5-5 part of polyvinylpyrrolidone, 0.05-5 part of hyaluronic acid and 0.05-5 part of chondroitin sulfate; the active substance is self-made stem cell culture supernatant freeze-dried powder; and the solvent is sterile water. The liquid woundplast belongs to the technical field of medical dressings. The liquid woundplast based on stem cell culture supernatant can quickly form a film and slowly release active substances, and has the functions of continuously and efficiently repairing wounds and inhibiting scar formation.

The invention provides a novel preparation method of liposome, aiming at solving the defect in the process that the liposome is prepared by the medicine which is easy to oxidize, hydrolyze and denature, wherein the liposome is higher in encapsulation ratios to various medicines. The preparation method of the liposome comprises the following steps of: a. dissolving a lipidic substance and a lipophilic substance which form into the liposome into an organic solvent to form into an oil phase solution (O); b. dissolving a water-soluble substance to be encapsulated into water to form into a water phase solution (W); c. mixing and emulsifying the two solutions according to a proper proration to obtain a W/O type emulsion (comprising micro emulsion and reversed micelle); d. drying the obtained W/O type emulsion in a freezing way to remove a solvent so as to obtain a freeze-dried product; and e. hydrating the obtained freeze-dried product to obtain the liposome. The novel preparation method is simple in technical process and easy to implement.

The invention discloses a collagen skin care product for improving skin wrinkles, which is prepared from the following raw materials in parts by weight: 5 to 15 parts of active plants, 10 to 15 parts of glycerol, 1 to 2 parts of butanediol, 7 to 13 parts of active additives, 5 to 10 parts of collagen and 30 to 40 parts of deionized water, the skin care product is prepared by crushing, mixing, foaming and stirring, secondary mixing, sterilizing, collagen adding and final mixing, during preparation, skin care product production equipment is adopted, so that production is improved, collection is convenient, and the finally obtained skin care product has collagen activity and collagen production promoting activity, and the problem of wrinkles caused by skin aging along with age increase can be effectively improved.

The invention discloses a pig feed additive as well as a preparation method and application thereof. The pig feed additive is prepared by uniformly mixing a phytase compound and microorganism composite powder, wherein the mass ratio of the phytase compound to the microorganism composite powder is (3-4) to (6-7); the phytase compound can ensure activated phytase with sufficient concentration to quickly degrade phytate in the feed after the pig feed additive is taken in, so as to alleviate the anti-nutrition effect of the phytate; bacillus subtilis in the microorganism composite powder continuously synthesizes various beneficial substances including the phytase in the subsequent process after being subjected to rehydration activation for a period of time. The pig feed additive provided by the invention can remarkably promote the growth of barren sow; due to the addition of the microorganism composite powder, after being fed for a long time, the intestinal environment of the barren sow can be improved, the subsequent healthy growth and growth rate of the barren sow are facilitated, meanwhile, the feed additive has good storage effect.

The invention belongs to the technical field of special methods of collecting honey, and particularly discloses a bee raising method. The method comprises the following steps that 1, honey collectingequipment is prepared, wherein the honey collecting equipment comprises a separating cylinder and a rotating cylinder, a drive shaft is rotationally connected into the separating cylinder and fixedlyconnected with the rotating cylinder, a separation through hole is formed in the side wall of the rotating cylinder, and a driving mechanism is connected to the drive shaft; 2, sealing wax on a spleenplate is cut off; 3, the spleen plate is put into the rotating cylinder and fixed, and then the driving mechanism is started; 4, the interior of the rotating cylinder is heated, wherein the heating temperature is 40-55 DEG C; 5, a gap between the rotating cylinder and the separating cylinder is cooled, wherein the cooling temperature is -10-5 DEG C; 6, silica gel dry particles are put into the gap between the rotating cylinder and the separating cylinder; 7, after 10-15 min, obtained honey is poured out and filtered, the silica gel dry particles are filtered out, and then the honey is collected and packaged. The problem that in the prior art, when the honey is taken, beewax is mixed in the honey is solved.