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32results about How to "Prevent denaturation and inactivation" patented technology
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Preparation method and application of sericin hydrogel
ActiveCN103951831AGood natural propertiesOvercoming the challenge of high biological properties of sericinNervous disorderPeptide/protein ingredientsCross-linkDisease
The invention discloses a preparation method of sericin hydrogel, the method is as follows: first, weighting domestic silkworm fibroin deletion form mutation variety silkworm cocoon for LiBr or LiCl extraction and dialysis and purification to obtain a non-degradable sericin water solution with a mass percentage concentration of 0.1-4%; concentrating the sericin water solution to 1.5-10%, adding a cross-linking agent to the concentrated sericin water solution (wherein 2-500muL of the cross-linking agent is added into each L of the sericin water solution), fully mixing, and placing at 4-45 DEG C for 5 seconds to 36 hours to obtain the sericin hydrogel. The sericin hydrogel has biological activity and multiple functions, can be used as carrying growth factors, drugs and cell carriers treatment, and can be applied in repair of a variety of soft tissue injuries, including but not limited to skin damages, muscle damages, injuries of blood vessel, nerve injuries, myocardial damages, and the like, and treatment of diseases.
Owner:XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
Blood heavy metal ion adsorbent, preparation method thereof and blood perfusion device
InactiveCN104984736AReduce riskPrevent denaturation and inactivationOther blood circulation devicesOther chemical processesSpecific adsorptionMagnetite Nanoparticles
The invention discloses a blood heavy metal ion adsorbent. With magnetic nano materials being carriers, the surfaces of the carriers are wrapped with ligand active groups in a modification mode, wherein the ligand active groups are capable of being combined with heavy metal ions and coordination compounds. The magnetic nano materials are magnetic iron-based compounds MFe2O4, wherein M is Fe2+ or Co2+ or Ni2+ or Zn2+ or Mg2+. The ligand active groups are one or more of -NH2, -SH2, -COOH and -OH. The invention further provides a preparation method of the blood heavy metal ion adsorbent and a blood perfusion device made from the blood heavy metal ion adsorbent. The surfaces of the magnetic nano-particles are modified, so that the blood heavy metal ion adsorbent is obtained and used for performing efficient selective or specific adsorption on one kind of heavy metal, it is avoided that because non-specific adsorption is performed on microelements in blood, the protein denatures and becomes inactive, and influences on the blood by the adsorbent and the risk of organism systematic complications are greatly reduced.
Owner:CENT SOUTH UNIV
Novel preparation method of solid lipid nanoparticles
InactiveCN102151250ALong term storageSimple preparation processPowder deliveryLyophilised deliveryLipid formationOrganic solvent
The invention discloses a novel preparation method of solid lipid nanoparticles (SLN) (including nanostructured lipid carriers NLC), solving the unstable problem of easiness in aggregation, agglomeration, and the like of the solid lipid nanoparticles (including NLC). The preparation method comprises the steps of: a, dissolving lipid matters and lipotrophic matters (including medicines) in an organic solvent (such as tertiary butanol) capable of being mixed and dissolved with water to form an oil phase (O), or solubilizing hydrophilic matters (including medicines) in an organic solvent (O) capable of being mixed and dissolved with water by using a surfactant, wherein the lipid matters and the lipotrophic matters are used for forming the solid lipid nanoparticles (including NLC); b, dissolving water-soluble matters in water to form a water phase (W); c, injecting the oil phase (O) into the water phase (W) under stirring condition according to a proper volume proportion to obtain a solid nanoparticle dispersing solution; d, freezing and drying the obtained dispersing solution to remove the solvent to obtain a freeze-dried product; and e, hydrating the obtained freeze-dried product to obtain the solid lipid nanoparticles (including NLC). The preparation method is simple in procedures and easy to implement.
Owner:王汀 +1
Aseptic collagen liquid with biological activity and preparation method thereof
PendingCN105985429APrevent denaturation and inactivationShorten the time of purificationCosmetic preparationsConnective tissue peptidesUltrafiltrationDialysis
The invention discloses an aseptic collagen liquid with biological activity and a preparation method thereof. The preparation method of the aseptic collagen liquid comprises degreasing, slicing, disinfecting and crushing at a low temperature animal tendons, carrying out acid dissolution and enzymolysis, carrying out filtration to remove residues, and carrying out salting-out, purification, loading, sealing, refrigeration and low temperature irradiation sterilization to obtain the aseptic collagen liquid. The preparation method realizes low temperature irradiation, keeps collagen activity, and prevents collagen inactivation and denaturation or cross-linking reaction under irradiation so that functions are not influenced. The preparation method utilizes ultrafiltration purification to replace the traditional dialysis purification, shortens purification time, utilizes an automatic system to replace the traditional semi-automatic operation, prevents human hand pollution and guarantees product cleanliness, purity and bioactivity.
Owner:GUANGZHOU TRAUER BIOTECH
Preparation method of high-purity human coagulation factor IX
InactiveCN105175486AReduce lossesPrevent denaturation and inactivationPeptide preparation methodsUltrafiltrationPolyethylene glycol
The invention relates to a preparation method of a high-purity human coagulation factor IX, which comprises the following steps: melting refrigerated plasma, and carrying out low-temperature centrifugation; adsorbing with a DEAE (diethylaminoethanol) Sephadex A-50 gel to remove the coagulation factor IX in the cold-glue plasma; removing impure proteins in the solution by using polyethyleneglycol; carrying out S / D virus inactivation; carrying out anion exchange column chromatography to obtain a purified coagulation factor IX solution; passing through a heparin affinity column for further chromatography to obtain a high-purity coagulation factor IX solution; carrying out ultrafiltration, dialysis and concentration, and adding arginine hydrochloride and glycinate as protective agents; filtering through a 20nm filter element to remove viruses; carrying out freeze-drying; and carrying out dry heat virus inactivation. The protein protective agents are added during the gel adsorption, column chromatography and ultrafiltration dialysis, thereby lowering the activation probability of the FIX product thrombin and enhancing the qualification rate of the product. The technique has high product yield; the FIX specific activity can reach 150 IU / mg or so which is much higher than that of the traditional product; and by performing the three-step virus inactivation, the product is safe and reliable to use.
Owner:上海洲跃生物科技有限公司
Bacteriocin, and preparation method and application thereof
InactiveCN104987370ACurb corruptionEnhanced inhibitory effectAntibacterial agentsPeptide/protein ingredientsStaphylococcusPseudomonas putida
The invention provides a bacteriocin, and a preparation method and an application thereof. The bacteriocin belongs to broad spectrum bacteriocin, and an antibacterial spectrum comprises Gram-negative bacteria and Gram-positive bacteria, such as Pseudomonas putida, Bacillus subtilis, Listeria monocytogenes, Shigella flexneri, Staphylococcus citreus, Escherichia coli and salmonella.
Owner:ZHEJIANG GONGSHANG UNIVERSITY
Bacterial strain for preparing high efficiency capsule glycoprotein immunomodulator, screening and application thereof
InactiveCN1524950AIncrease productionHigh activityBacteriaPeptide/protein ingredientsK pneumoniaeMicroorganism
The invention discloses a bacterial strain for preparing highly effective capsula glucoprotein immuno-modulatory agent, its selection and use thereof, which relates to a strain of K. peneumoniae and its screen selecting method, and the method for preparing capsula glucoprotein by using the strain, wherein the K. peneumoniae capsula glucoprotein immuno-modulatory agent is prepared through the steps of strain screening, culture, cracking, crude extracting and purification, thus overcoming the problem of drug tolerance for antibiotics when used for treating infection.
Owner:TIANJIN MEDICAL UNIV
LLarge scale human urine protein collection method
InactiveCN103509104ARealize online adsorptionExpand the scope of collectionAlbumin peptidesPeptide preparation methodsPro proteinProtein activity
The invention discloses a large scale human urine protein collection method. The method comprises the following steps: placing absorption agents, which are used for absorbing urine proteins and have been packaged, in the places where urine will pass through to make the absorption agent be able to absorb the urine proteins in the urine; collecting the absorption agents which have absorbed the urine proteins; subjecting the collected absorption agents to a pretreatment; refrigerating and storing the pre-treated absorption agents; and transporting the absorption agents to a processing factory in batches to carry out urine protein desorption process to produce corresponding urine protein raw materials or urine protein products. The large scale human urine protein collection method is suitable for the mass industrial urine protein production, improves the processing amount of urine protein, reduces the transportation cost of absorption agents and the production cost of urine proteins, furthermore, the absorption agents containing urine proteins are subjected to a pre-treatment before storage, thus the urine protein activity is preserved, the urine protein degradation is reduced, the microorganism generation is reduced, so that the urine protein product quality is improved.
Owner:YANGZHOU AIDEA BIOTECH
Poultry freezing and fresh-keeping method
ActiveCN111213707AImprove textureAdd flavorFood ingredient as antioxidantFood freezingBiotechnologyAnimal science
The invention discloses a poultry freezing and fresh-keeping method. The method comprises the following steps: S1, acid discharging at low temperature; S2, pre-impregnation: putting meat blocks into afreezing protection solution, and performing ultrasonic impregnation at 0-2 DEG C for 1.5-3h, wherein the freezing protection solution comprises the following raw materials in parts by mass: 1 to 1.5parts of egg white protein peptide, 0.01 to 0.03 part of antifreeze protein, 0.5 to 1 part of tea polyphenol, 4 to 6 parts of sodium alginate, 0.5 to 1 part of lysozyme, 0.2 to 0.4 part of CITREM and100 parts of water; S3, magnetic field-assisted freezing: in a magnetic field environment with the magnetic induction intensity of 10-30Gs and the frequency of 50-1000Hz, continuously soaking the meat blocks into the freezing protection solution for pre-freezing for 25-50min at -3 to 0 DEG C, taking out the meat blocks, and performing freezing at -35 to -28 DEG C until the central temperature ofthe meat blocks is lower than -20 DEG C; and S4, cold storage. According to the invention, a protection liquid impregnation way and a magnetic field-assisted technology are adopted to perform synergetic quick-freezing on poultry, so that the poultry is kept in a supercooled state, phase change is controlled, ice crystal growth and recrystallization are effectively inhibited, the integrity of cellsand tissues is kept, spoilage bacteria are killed or inhibited, and lipid oxidation is prevented.
Owner:安徽鲜森绿色食品有限公司
Production of elaioplast
InactiveCN1895223ALong term storageSimple processPharmaceutical non-active ingredientsLiposomal deliveryOrganic solventFreeze-drying
A process for preparing the liposome of the medicine easy to be oxidized, hydrolyzed and modified includes such steps as dissolving the ester substance and the lipophilic substance to be coated in organic solvent to form oil phase, dissolving the hydrophilic substance to be coated in water to form internal water phase, mixing them, emulsifying to become W1 / O type emulsion, mixing it with proper aqueous solution, emulsifying to become W1 / O / W3 type complex emulsion, freeze drying, and hydrating to become liposome.
Owner:SHENYANG PHARMA UNIVERSITY
Method for collecting urine protein in large scale from human urine
InactiveCN106699876AImprove adsorption capacityExpandable collection rangeAlbumin peptidesPeptide preparation methodsMicroorganismBlood plasma
The invention relates to a method for collecting urine protein in a large scale from human urine. The method is especially suitable for large-scale use, and comprises the following steps: manufacturing a urine filtering device by use of an anion exchanger, and placing the urine filtering device in a urinal funnel or a urinal channel for carrying the urine flowing through the filtering device, wherein the shape and size of the urine filtering device are adaptive to those of the urinal funnel or the urinal channel; and recycling the urine filtering device at fixed time, desorbing and extracting the anion exchanger in concentration, extracting the component containing the urine protein, and performing the regeneration treatment on the anion exchanger so that the anion exchanger can be further used. The urine protein with various indexes achieving the pharmacopoeia requirements can be prepared through the subsequent purification treatment, and the urine protein can be used as the substitute goods of the human plasma source protein in multiple fields. The more important is that the corresponding pretreatment is performed before performing the treatment on the adsorbent absorbing the urine protein, so that the activity of the urine protein is guaranteed, the degradation of the urine protein and the propagation of microorganisms are reduced, and the quality of the urine protein product is improved.
Owner:GUANGDONG TECHPOOL BIO-PHARMA CO LTD
Preparation method for human antithrombin III
InactiveCN105315365AReduce the probability of activationImprove pass ratePeptide preparation methodsProtease inhibitorsUltrafiltrationPolyethylene glycol
The invention discloses a preparation method for human antithrombin III (AT-III). The preparation method comprises the following steps: (1) precipitation and dissolution of a blood plasma fraction IV; (2) polyethylene glycol (PEG) precipitation and impure protein removing; (3) DEAE Sephadex A-50 gel adsorption; (4) S / D virus inactivation; (5) heparin affinity column chromatography; (6) ultrafiltration and concentration; (7) addition of a stabilizer and regulation; (8) nanofilm virus-removing filtration; (9) sterilizing filtration; (10) freeze-drying; (11) dry and fever virus inactivation. According to the preparation method, blood coagulation factors, which remain in the raw materials and are depended by vitamin K, are removed, so as to greatly lower the possibility of protein activation in the production process, and improve the product qualification ratio; PEG is adopted for the impure protein removing, so as to reduce the load of a chromatographic column; a liquid obtained from lyophilized powder re-dissolution is clear, transparent, and free of protein precipitation and opalescence; through adoption of a three-step virus inactivation method, the safety of human AT-III for clinical use can be greatly improved.
Owner:上海洲跃生物科技有限公司
Method for separating and purifying human serum albumin from Cohn component V supernatant
PendingCN109627322AHigh purityPrevent denaturation and inactivationSerum albuminPeptide preparation methodsUltrafiltrationEthanol precipitation
The invention provides a method for separating and purifying human serum albumin from a Cohn component V supernatant, which comprises the following steps of: loading a supernatant of the Cohn component V into a chromatographic column containing an anion exchange chromatographic medium, eluting the chromatographic column with eluent, collecting the eluent to obtain human serum albumin; according tothe method, a novel idea of separating and purifying albumin by using the low-temperature ethanol precipitation component V supernatant as a raw material is put forward for the first time, ethanol isnot removed by adopting an ultrafiltration process, so that the using amount of a buffer solution is greatly reduced, the operation time is reduced, the method avoids the problem of denaturation andinactivation of the protein in a higher ethanol solution for a long time, and has the advantages of simple operation, low cost, short process time consumption, high protein activity yield and the like. The method can further recover the albumin in the supernatant of the component V, effectively improve the utilization rate of the plasma and reduce the cost, and has important value for practical application.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI
Preparation method of liquid woundplast based on stem cell culture supernatant
InactiveCN112618783AGuaranteed homogeneityImprove structural formBandagesPyrrolidinonesStem cell culture
The invention discloses a preparation method of a liquid woundplast based on stem cell culture supernatant. The liquid woundplast comprises a film-forming solution, an active substance and a solvent; the film-forming solution comprises 2-10 parts of chitosan hydrochloride, 0.5-5 part of polyvinylpyrrolidone, 0.05-5 part of hyaluronic acid and 0.05-5 part of chondroitin sulfate; the active substance is self-made stem cell culture supernatant freeze-dried powder; and the solvent is sterile water. The liquid woundplast belongs to the technical field of medical dressings. The liquid woundplast based on stem cell culture supernatant can quickly form a film and slowly release active substances, and has the functions of continuously and efficiently repairing wounds and inhibiting scar formation.
Owner:海南优尼科尔生物科技有限公司
Method for screening prostate cancer PSA diagnosis gray region serum marker
InactiveCN113740449APrecise screeningSimplifying the procedure for detecting prostate cancerComponent separationHyperplasiaProstatic biopsy
The invention discloses a method for screening a prostate cancer PSA diagnosis gray region serum marker. The method comprises the following steps of S10, collecting a sample; S20, carrying out metabonomics pretreatment; S30, arranging a to-be-tested sample analysis operation sequence; S40, carrying out a non-targeted metabonomics experiment; S50, qualitatively detecting the original data of metabolites; S60, screening differential metabolites among the samples to be detected; S70, carrying out metabolic pathway analysis; S80, screening serum metabolites, and determining candidate diagnostic markers; and S90, determining a diagnostic marker. The invention provides a biomarker which is efficient, minimally invasive and capable of accurately screening prostatic cancer and distinguishing prostatic hyperplasia, so that the procedure for detecting the prostatic cancer is simplified, and complications caused by rectal ultrasound guided prostatic biopsy diagnosis are avoided.
Owner:徐蓓 +3
Novel preparation method of liposome
InactiveCN102151246ALong term storageSimple processPharmaceutical non-active ingredientsLiposomal deliveryOrganic solventFreeze-drying
The invention provides a novel preparation method of liposome, aiming at solving the defect in the process that the liposome is prepared by the medicine which is easy to oxidize, hydrolyze and denature, wherein the liposome is higher in encapsulation ratios to various medicines. The preparation method of the liposome comprises the following steps of: a. dissolving a lipidic substance and a lipophilic substance which form into the liposome into an organic solvent to form into an oil phase solution (O); b. dissolving a water-soluble substance to be encapsulated into water to form into a water phase solution (W); c. mixing and emulsifying the two solutions according to a proper proration to obtain a W / O type emulsion (comprising micro emulsion and reversed micelle); d. drying the obtained W / O type emulsion in a freezing way to remove a solvent so as to obtain a freeze-dried product; and e. hydrating the obtained freeze-dried product to obtain the liposome. The novel preparation method is simple in technical process and easy to implement.
Owner:王汀 +1
Collagen skin care product for improving skin wrinkles
PendingCN113057932AKeep skin moisturizedIncrease productionCosmetic preparationsToilet preparationsChemistryWrinkle
The invention discloses a collagen skin care product for improving skin wrinkles, which is prepared from the following raw materials in parts by weight: 5 to 15 parts of active plants, 10 to 15 parts of glycerol, 1 to 2 parts of butanediol, 7 to 13 parts of active additives, 5 to 10 parts of collagen and 30 to 40 parts of deionized water, the skin care product is prepared by crushing, mixing, foaming and stirring, secondary mixing, sterilizing, collagen adding and final mixing, during preparation, skin care product production equipment is adopted, so that production is improved, collection is convenient, and the finally obtained skin care product has collagen activity and collagen production promoting activity, and the problem of wrinkles caused by skin aging along with age increase can be effectively improved.
Owner:李雪云
Adipose tissue-derived stromal cell cryopreservation liquid and cryopreservation method thereof
ActiveCN112075417AImprove survival rateGuaranteed survival rateDead animal preservationSodium phosphatesBilobalides
The invention provides an adipose tissue-derived stromal cell cryopreservation liquid. The adipose tissue-derived stromal cell cryopreservation liquid is prepared from the following raw materials: a DMEM / F12 culture medium, 50 to 60 [mu] g / mL of ethylene glycol monomethyl ether, 1.5 to 4.0 mg / mL of acetylchitosamine, 25 to 30 ng / mL of bilobalide, 35 to 45 ng / mL of sodium pyrophosphate, 80 to 90 [mu] g / mL of alfalfa saponin and 100 to 110 [mu] g / mL of lipoic acid. According to the adipose tissue-derived stromal cell cryopreservation liquid provided by the invention, animal-derived serum and DMSO are not added, and the survival rate of resuscitated cells is high. The ethylene glycol monomethyl ether and the acetylchitosamine are added into the cryopreservation liquid, so that cells are not damaged, the permeability of the cells is improved, the osmotic pressure inside and outside the cells is maintained to be stable, and the activity of the cells is maintained. The bilobalide and the sodium pyrophosphate are added and compounded for use, so that denaturation and inactivation of cell protein molecules are prevented, and rapid activity recovery of cells after long-time cryopreservationis facilitated. Alfalfa saponin and lipoic acid are also added into the cryopreservation liquid, free radicals generated in the cryopreservation process are removed, and the antioxidation effect is achieved. The invention further provides a cryopreservation method of the adipose tissue-derived stromal cells, and the method is simple and convenient and easy to operate.
Owner:GUANGDONG CELL BIOTECHNOLOGY CO LTD
Breaking method of desert algae cells
The invention discloses a breaking method of desert algae cells. The breaking method of the desert algae cells comprises the steps that the desert algae cell sap is subjected to ultrasonic breaking treatment, the ultrasonic frequency is 60 kHz, the power ranges from 300 to 800 W, time of each treatment ranges from 15 to 35 min, and treatment is carried out for 60 to 90 times. According to the breaking method of the desert algae cells, the ultrasonic breaking method is adopted, and the method is related with ultrasonic time, cell sap concentrations, and sound frequency and sound energy of ultrasonic waves. Ultrasonic wave extracting process parameters are researched, the process technology with the high extraction rate is optimized, the desert algae cells can be effectively and efficientlybroken, ultrasonic treatment is carried out under the ice-bath condition, the local high temperature phenomenon generated by the ultrasonic treatment can be avoided, meanwhile, the ultrasonic mode selects the intermittent manner, and denaturation and inactivation of heat-reactivity active matter can be prevented.
Owner:新疆拓必达科技发展有限公司
Method for extracting and separating eugenol and syringic acid by using aqueous two-phase system
ActiveCN113372202AEasy to operateLow costEther separation/purificationOrganic compound preparationEugenolSolvent
The invention discloses a method for extracting and separating eugenol and syringic acid by using an aqueous two-phase system, which comprises the following steps: adding an aqueous solution containing eugenol and syringic acid into an extracting solvent consisting of a natural eutectic solvent and tert-butyl alcohol, and carrying out aqueous two-phase extraction to respectively obtain eugenol and syringic acid. The method disclosed by the invention is simple to operate, low in cost, green and efficient, eugenol and syringic acid can be enriched and recovered from the aqueous solution, and the problems of complicated operation, high cost, high equipment requirement, difficulty in recovery of bioactive substances and high toxicity and high volatility of an extraction solvent in a traditional extraction and separation method are solved.
Owner:SOUTH CHINA UNIV OF TECH
Pig feed additive as well as preparation method and application thereof
InactiveCN110169509APrevent spoilageImprove deteriorationFood processingAnimal feeding stuffMicroorganismPhytase
The invention discloses a pig feed additive as well as a preparation method and application thereof. The pig feed additive is prepared by uniformly mixing a phytase compound and microorganism composite powder, wherein the mass ratio of the phytase compound to the microorganism composite powder is (3-4) to (6-7); the phytase compound can ensure activated phytase with sufficient concentration to quickly degrade phytate in the feed after the pig feed additive is taken in, so as to alleviate the anti-nutrition effect of the phytate; bacillus subtilis in the microorganism composite powder continuously synthesizes various beneficial substances including the phytase in the subsequent process after being subjected to rehydration activation for a period of time. The pig feed additive provided by the invention can remarkably promote the growth of barren sow; due to the addition of the microorganism composite powder, after being fed for a long time, the intestinal environment of the barren sow can be improved, the subsequent healthy growth and growth rate of the barren sow are facilitated, meanwhile, the feed additive has good storage effect.
Owner:何杰梅
Method for preparing microspheres through oil in nano-particle suspension-oil in oil-water in oil
InactiveCN102895193BAvoid defects with low encapsulation efficiencyReduce inflammationPowder deliveryPharmaceutical non-active ingredientsCell adhesionPrill
The invention discloses a method for preparing microspheres through oil in nano-particle suspension-oil in oil-water in oil (W / O / O / S) and belongs to the technical field of pharmacy. The method includes adding medicine aqueous solution to a polymer organic solution for emulsification to obtain an emulsion, adding the emulsion into a hydrophilic oil phase, stirring, mixing, choosing the nano-particle suspension to serve as a surface active agent ball, finally performing hardening in another large aqueous phase, removing the organic solvent, and collecting the microspheres. The prepared microspheres comprise, by weight, 0.1%-40% of medicines, 9.9%-96% of nano-particles, 90%-3.65% of polymers and 0-30% of pharmaceutic adjuvants. According to the method for preparing the microspheres through oil in nano-particle suspension-oil in oil-water in oil (W / O / O / S), the problem of poor tissue compatibility caused by the fact that the microspheres are prepared through conventional methods of water in oil (W / O), water in oil in water (W / O / W) and solid in oil in oil (S / O / O) is solved, the surfaces of the prepared microspheres are assembled with the nano-particles, the prepared microspheres have effect of enhancing cell adhesion, inflammation and microencapsulation caused by local peracid and a hydrophobic material can be reduced, and the method can be applied to preparation of various medicine sustained-release or controlled-release microspheres and treatment of diseases.
Owner:SHANGHAI JIAO TONG UNIV
Method and Application of Surface Molecularly Imprinted Microspheres Prepared by Pickering Emulsion Polymerization Based on Hydrophobic Hydroxyapatite Nano-stabilized Particles
ActiveCN106589366BIncrease profitSimple stepsOther chemical processesAlkali metal oxides/hydroxidesBlotMaterials science
The invention discloses a method for preparing surface molecular imprinting microspheres through polymerization of Pickering emulsion based on hydrophobic hydroxylapatite nanometer stable particles and application. The method for preparing the surface molecular imprinting microspheres comprises the steps that the hydrophobic hydroxylapatite nanometer particles are used as stable particles, protein is used as template molecules, polymeric microspheres are prepared through self-assembly of dopamine monomers in an aqueous phase, the template molecules are further removed through eluent, and the surface molecular imprinting microspheres can be obtained. Print sites are arranged on the surfaces of the surface molecular imprinting microspheres, the surface molecular imprinting microspheres can be in full contact with target molecules in a solution, efficient specific adsorption on the target molecules can be achieved, and the surface molecular imprinting microspheres can be widely applied to specific adsorption and separation of protein in a biosystem. According to the method, preparation is easy to grasp, preparation is easy and convenient, cost is low, and large-scale industrial production is greatly facilitated.
Owner:CENT SOUTH UNIV
A kind of extraction system of plant active substance
ActiveCN113101692BAvoid cloggingGuaranteed extraction concentrationSolid solvent extractionEnvironmental engineeringMechanical engineering
Owner:SOUTH CHINA AGRI UNIV
A method of raising bees
Owner:武汉市大兴蜂业有限责任公司
Jelly with nectarine, pomegranate and collagen ferment as well as preparation method and application of jelly
ActiveCN114431440AIncreased SOD contentImprove heat resistanceFood ingredient functionsBiotechnologyPectinase
The invention relates to ferment jelly, in particular to nectarine and pomegranate collagen ferment jelly as well as a preparation method and application thereof. Mixing fruits and vegetables, adding cane sugar, pulping, pasteurizing, and cooling to room temperature to obtain fruit and vegetable pulp; adding cellulase and pectinase, and performing enzymolysis to obtain enzymatic hydrolysate; adding primary zymophyte, and fermenting to obtain primary fermentation liquor; adding secondary zymophyte composed of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus paracasei and streptococcus thermophilus for fermentation, and filtering to remove impurities to obtain secondary fermentation liquor; the preparation method comprises the following steps: adding honey peach and pomegranate collagen enzyme into a stirrer, adding a protective agent which comprises trehalose, xylitol, amino acid, glycerol and hydroxypropyl-beta-cyclodextrin, adding collagen peptide and pre-dissolved agar, uniformly mixing, adding cheese powder, uniformly mixing, and cooling to obtain the honey peach and pomegranate collagen enzyme jelly. The nectarine and pomegranate collagen enzyme jelly prepared by the preparation method disclosed by the invention is high in SOD activity, relatively high in heat resistance and low temperature resistance, capable of preventing enzyme inactivation and long in shelf life.
Owner:广州市巴菲巴健康产业有限公司
Bee raising method
The invention belongs to the technical field of special methods of collecting honey, and particularly discloses a bee raising method. The method comprises the following steps that 1, honey collectingequipment is prepared, wherein the honey collecting equipment comprises a separating cylinder and a rotating cylinder, a drive shaft is rotationally connected into the separating cylinder and fixedlyconnected with the rotating cylinder, a separation through hole is formed in the side wall of the rotating cylinder, and a driving mechanism is connected to the drive shaft; 2, sealing wax on a spleenplate is cut off; 3, the spleen plate is put into the rotating cylinder and fixed, and then the driving mechanism is started; 4, the interior of the rotating cylinder is heated, wherein the heating temperature is 40-55 DEG C; 5, a gap between the rotating cylinder and the separating cylinder is cooled, wherein the cooling temperature is -10-5 DEG C; 6, silica gel dry particles are put into the gap between the rotating cylinder and the separating cylinder; 7, after 10-15 min, obtained honey is poured out and filtered, the silica gel dry particles are filtered out, and then the honey is collected and packaged. The problem that in the prior art, when the honey is taken, beewax is mixed in the honey is solved.
Owner:武汉市大兴蜂业有限责任公司
Production method of elaioplast
InactiveCN1895223BLong term storageSimple processPharmaceutical non-active ingredientsLiposomal deliveryOrganic solventFreeze-drying
A process for preparing the liposome of the medicine easy to be oxidized, hydrolyzed and modified includes such steps as dissolving the ester substance and the lipophilic substance to be coated in organic solvent to form oil phase, dissolving the hydrophilic substance to be coated in water to form internal water phase, mixing them, emulsifying to become W1 / O type emulsion, mixing it with proper aqueous solution, emulsifying to become W1 / 0 / W3 type complex emulsion, freeze drying, and hydrating to become liposome.
Owner:SHENYANG PHARMA UNIVERSITY
Bacterial strain for preparing high efficiency capsule glycoprotein immunomodulator, screening and application thereof
InactiveCN1320104CIncrease productionHigh activityAntibacterial agentsBacteriaK pneumoniaeMicroorganism
The invention discloses a bacterial strain for preparing highly effective capsula glucoprotein immuno-modulatory agent, its selection and use thereof, which relates to a strain of K. peneumoniae and its screen selecting method, and the method for preparing capsula glucoprotein by using the strain, wherein the K. peneumoniae capsula glucoprotein immuno-modulatory agent is prepared through the steps of strain screening, culture, cracking, crude extracting and purification, thus overcoming the problem of drug tolerance for antibiotics when used for treating infection.
Owner:TIANJIN MEDICAL UNIV
A kind of fat mesenchymal stem cell cryopreservation liquid and cryopreservation method thereof
ActiveCN112075417BImprove survival rateGuaranteed survival rateDead animal preservationSodium phosphatesChemistry
The invention discloses a fat mesenchymal stem cell cryopreservation solution, which is composed of the following raw materials: DMEM / F12 medium, 50-60 μg / mL of ethylene glycol monomethyl ether, 1.5-4.0 mg / mL of acetylglucosamine, ginkgo Lactone 25-30ng / mL, sodium pyrophosphate 35-45ng / mL, alfalfa saponin 80-90μg / mL, lipoic acid 100-110μg / mL. The invention provides a fat-derived mesenchymal stem cell cryopreservation solution, which does not add animal serum and DMSO, and has a high cell survival rate after resuscitation. Adding ethylene glycol monomethyl ether and acetyl glucosamine to the cryopreservation solution of the present invention has no damage to cells, improves cell permeability, maintains stable osmotic pressure inside and outside cells, and helps cells maintain activity. The combination of bilobalide and sodium pyrophosphate is added to prevent the denaturation and inactivation of cell protein molecules, and help cells quickly recover their activity after long-term freezing. The cryopreservation solution of the present invention is also added with alfalfa saponin and lipoic acid to remove free radicals generated during the cryopreservation process and has an anti-oxidation effect. The invention also provides a cryopreservation method of adipose-derived mesenchymal stem cells, which is simple and easy to operate.
Owner:GUANGDONG CELL BIOTECHNOLOGY CO LTD
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