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Method for extracting and separating eugenol and syringic acid by using aqueous two-phase system

A two-phase aqueous system, eugenol technology, applied in chemical instruments and methods, ether separation/purification, carboxylate preparation, etc., can solve the problems of poor biodegradability of ionic liquids, large-scale application limitations, and difficult post-processing, etc. Achieve good extraction and separation effects, avoid denaturation and inactivation, and avoid post-processing difficulties

Active Publication Date: 2021-09-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, inorganic salts may cause ion exchange between the two phases, resulting in difficulties in post-processing. The biodegradability of ionic liquids is poor. They are not really green solvents, and they are expensive, which limits their large-scale applications.

Method used

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  • Method for extracting and separating eugenol and syringic acid by using aqueous two-phase system
  • Method for extracting and separating eugenol and syringic acid by using aqueous two-phase system
  • Method for extracting and separating eugenol and syringic acid by using aqueous two-phase system

Examples

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Effect test

Embodiment 1

[0035] A method for extracting and separating eugenol and syringic acid using a two-phase system, comprising the following steps:

[0036] 1) Mix 1 g of betaine and 5.7269 g of sucrose, and heat at 60°C for 2 hours to obtain a natural deep eutectic solvent;

[0037] 2) Add 1.5g of an aqueous solution containing eugenol and syringic acid to an extraction solvent composed of 1.3g of natural deep eutectic solvent and 2.2g of tert-butanol, shake at room temperature, and then place it in a constant temperature bath at 25°C for 4h , the system is completely phase-separated, the upper phase is tert-butanol phase containing eugenol, the lower phase is natural deep eutectic solvent phase containing syringic acid, and then pipette the separated upper and lower phase solutions, and add 1mL deionized Dilute with water, record the two-phase volume after mixing evenly, then analyze the content of eugenol and syringic acid with the high-performance liquid chromatography with ultraviolet dete...

Embodiment 2

[0047] A method for extracting and separating eugenol and syringic acid using a two-phase system, comprising the following steps:

[0048] 1) Mix 1g of betaine and 2.5975g of xylitol, and heat at 60°C for 2h to obtain a natural deep eutectic solvent;

[0049] 2) Add 1.5g of an aqueous solution containing eugenol and syringic acid to an extraction solvent composed of 1.3g of natural deep eutectic solvent and 2.2g of tert-butanol, shake at room temperature, and then place it in a constant temperature bath at 25°C for 4h , the system is completely phase-separated, the upper phase is tert-butanol phase containing eugenol, the lower phase is natural deep eutectic solvent phase containing syringic acid, and then pipette the separated upper and lower phase solutions, and add 1mL deionized After diluting with water, record the volume of the two phases after mixing evenly, and analyze the content of eugenol and syringic acid by high performance liquid chromatography with ultraviolet dete...

Embodiment 3

[0052] A method for extracting and separating eugenol and syringic acid using a two-phase system, comprising the following steps:

[0053] 1) Mix 1 g of betaine and 1.0147 g of urea, heat at 60° C. for 2 hours to obtain a natural deep eutectic solvent;

[0054] 2) Add 1.5g of an aqueous solution containing eugenol and syringic acid to an extraction solvent composed of 1.3g of natural deep eutectic solvent and 2.2g of tert-butanol, shake at room temperature, and then place it in a constant temperature bath at 25°C for 4h , the system is completely phase-separated, the upper phase is tert-butanol phase containing eugenol, the lower phase is natural deep eutectic solvent phase containing syringic acid, and then pipette the separated upper and lower phase solutions, and add 1mL deionized After diluting with water, record the volume of the two phases after mixing evenly, and analyze the content of eugenol and syringic acid by high performance liquid chromatography with ultraviolet ...

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Abstract

The invention discloses a method for extracting and separating eugenol and syringic acid by using an aqueous two-phase system, which comprises the following steps: adding an aqueous solution containing eugenol and syringic acid into an extracting solvent consisting of a natural eutectic solvent and tert-butyl alcohol, and carrying out aqueous two-phase extraction to respectively obtain eugenol and syringic acid. The method disclosed by the invention is simple to operate, low in cost, green and efficient, eugenol and syringic acid can be enriched and recovered from the aqueous solution, and the problems of complicated operation, high cost, high equipment requirement, difficulty in recovery of bioactive substances and high toxicity and high volatility of an extraction solvent in a traditional extraction and separation method are solved.

Description

technical field [0001] The invention relates to the technical field of extraction and separation of phenolic compounds, in particular to a method for extracting and separating eugenol and syringic acid using a two-phase system. Background technique [0002] Natural deep eutectic solvents (NADES) are composed of naturally occurring hydrogen bond acceptors (such as choline, choline derivatives, etc.) and hydrogen bond donors (such as sugars, sugar alcohols, organic acids, amides, etc.) etc.), is a new type of green solvent that has only been developed in recent years. NADES contains large asymmetric ions with low lattice energy, and the melting point of the mixture can be much lower than that of the individual components through the hydrogen bond interaction between hydrogen bond acceptors and hydrogen bond donors. Compared with traditional organic solvents, NADES has the advantages of non-toxic, non-volatile, green and environmental protection, and its biocompatibility is ou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C41/38C07C43/23C07C67/58C07C69/92
CPCC07C41/38C07C67/58C07C43/23C07C69/92
Inventor 严宗诚尹康玲陈砺刘凤
Owner SOUTH CHINA UNIV OF TECH
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