Preparation method and application of sericin hydrogel

A sericin and hydrogel technology, applied in the field of biomedical composite materials, can solve the problems of fragile physical properties, high water solubility, and small molecular weight of sericin, achieve good biocompatibility, and the preparation method is simple and easy Effect

Active Publication Date: 2014-07-30
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biomaterials made purely with sericin are still limited to fragile two-dimensional sericin membranes, and true three-dimensional pure sericin hydrogels are still difficult to achieve.
The main reason is that the traditional sericin separation methods (such as high temperature method, alkali method) easily lead to the degradation of sericin protein, and the degraded sericin protein has small molecular weight, fragile physical properties, high water solubility, and is difficult to produce (including cross-linking) Hydrogels
Greatly restricted the application of sericin in tissue engineering

Method used

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  • Preparation method and application of sericin hydrogel

Examples

Experimental program
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Effect test

Embodiment 1

[0074] The preparation method of sericin hydrogel comprises the following steps:

[0075] 1. Selection of silkworm cocoons:

[0076] The cocoons of silkworm silk fibroin-deficient variety (purchased from the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, and the resources of such varieties are preserved in the National Silkworm Resource Preservation Center of the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences) are used as raw materials. The main chemical composition is: sericin

[0077] 2. Extraction and separation of sericin

[0078] 1) Weigh 1g of silkworm cocoons (purchased from Sericulture Research Institute of Chinese Academy of Agricultural Sciences) and cut into 1cm 2 The fragments were placed in a clean beaker, washed 3 times with ultrapure water, and centrifuged at 3500rpm for 5 minutes to remove water;

[0079] 2) Add 30-60 mL of LiBr aqueous solution with a concentration of 6 mol / L to the silkworm c...

Embodiment 2

[0126] The preparation method of the sericin aqueous solution in this embodiment is the same as that of Example 1, the difference is,

[0127] 1. Add 100 μL of 25% glutaraldehyde to every 1 mL of sericin aqueous solution;

[0128] 2. Mix thoroughly and place at 37° C. for 0.5 hour to obtain sericin hydrogel.

Embodiment 3

[0130] The preparation method of the sericin aqueous solution in this embodiment is the same as that of Example 1, the difference is,

[0131] 1. Add 2 μL of 25% glutaraldehyde to every 1 mL of sericin aqueous solution;

[0132] 2. After fully stirring and mixing, place at 37° C. for 0.5 hour to obtain sericin hydrogel.

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Abstract

The invention discloses a preparation method of sericin hydrogel, the method is as follows: first, weighting domestic silkworm fibroin deletion form mutation variety silkworm cocoon for LiBr or LiCl extraction and dialysis and purification to obtain a non-degradable sericin water solution with a mass percentage concentration of 0.1-4%; concentrating the sericin water solution to 1.5-10%, adding a cross-linking agent to the concentrated sericin water solution (wherein 2-500muL of the cross-linking agent is added into each L of the sericin water solution), fully mixing, and placing at 4-45 DEG C for 5 seconds to 36 hours to obtain the sericin hydrogel. The sericin hydrogel has biological activity and multiple functions, can be used as carrying growth factors, drugs and cell carriers treatment, and can be applied in repair of a variety of soft tissue injuries, including but not limited to skin damages, muscle damages, injuries of blood vessel, nerve injuries, myocardial damages, and the like, and treatment of diseases.

Description

technical field [0001] The invention relates to the field of biomedical composite materials, in particular to a preparation method and application of sericin hydrogel. The hydrogel has biological activity and multifunctionality, and can be used as a carrier of growth factors, drugs and cells, and can be applied to the repair of various soft tissue injuries and the treatment of diseases, including but not limited to skin injuries, muscle injuries, blood vessel injuries, Nerve damage, myocardial damage, etc. Background technique [0002] Hydrogels are three-dimensional network polymers that can swell in water and retain large amounts of water. As an important class of biomaterials, hydrogels have the characteristics of swelling and retaining a large amount of water, and have been widely used in the fields of biomedicine and tissue engineering. One type of hydrogel is prepared from natural biomaterials (such as collagen, alginate, and chitosan, etc.). This type of hydrogel is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/00C08J3/24A61L27/22A61L27/52A61L27/54A61L31/04A61L31/16A61L31/14
CPCC08H1/00A61L27/227A61L27/3604A61L27/3687A61L27/52A61L15/40A61L15/60C08L89/00C08J3/075C08J2389/00A61P17/00A61P21/00A61P25/00A61P43/00A61P9/00A61K38/1767A61K49/0056
Inventor 王琳张业顺黄雷刘佳王征李永奎杨文乞超
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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