A kind of sericin hydrogel and its preparation method and application

A technology of sericin and hydrogel, applied in the field of biomedical composite materials

Active Publication Date: 2019-05-21
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there has been no report that sericin extracted by high temperature or alkaline extraction has been prepared into injectable hydrogel by self-crosslinking

Method used

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  • A kind of sericin hydrogel and its preparation method and application
  • A kind of sericin hydrogel and its preparation method and application
  • A kind of sericin hydrogel and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] Embodiment 1 Preparation of sericin hydrogel provided by the present invention

[0108] Selection of cocoons:

[0109] Wild-type silkworm cocoons (provided by the Institute of Sericulture, Chinese Academy of Agricultural Sciences) are selected, and the main chemical components are: sericin and silk fibroin.

[0110] Step 1), extract sericin and make it into powder

[0111] 1. Weigh wild-type silkworm cocoons (100g and cut into 1cm 2 The fragments were placed in a clean beaker, washed 3 times with ultrapure water, and centrifuged at 3500rpm for 5 minutes to remove water;

[0112] 2. Add 3 to 4 L of Na with a molar concentration of 0.02 mol / L to the silkworm cocoon fragments obtained in step 1 2 CO 3 solution or 3-4L of deionized water, put the beaker into a constant temperature water bath at 100°C for 1 hour to dissolve the sericin protein and obtain a sericin protein solution;

[0113] 3. Transfer the solution obtained in step 2 into a centrifuge tube and centrifug...

Embodiment 2

[0137] Example 2 Preparation of sericin hydrogel provided by the present invention

[0138] In the present embodiment, the preparation method of sericin solution is the same as that of Example 1, except that step 4) and step 5) are carried out as follows:

[0139] 1. Dissolve hydrazide-modified silk protein powder in ddH 2 In O, the concentration is adjusted to about 200g / L to obtain the hydrazide-modified sericin solution;

[0140] 2. Dissolve oxidized dextran powder in ddH 2 O, the concentration was adjusted to about 200g / L to obtain an oxidized dextran solution;

[0141] 3. Add 5mL oxidized dextran solution to every 1mL sericin solution;

[0142] 4. After fully stirring and mixing, place at 4°C, 25°C, and 37°C respectively to obtain sericin hydrogel SDH-2.

Embodiment 3

[0143] Example 3 Preparation of sericin hydrogel provided by the present invention

[0144] The difference between the preparation method of sericin hydrogel in this example and the preparation method of Example 1 is that step 4) and step 5) are carried out as follows:

[0145] 1. Dissolve hydrazide-modified silk protein powder in ddH 2 In O, the concentration is adjusted to about 200g / L to obtain the hydrazide-modified sericin solution;

[0146] 2. Dissolve oxidized dextran powder in ddH 2 O, the concentration was adjusted to about 350g / L to obtain an oxidized dextran solution;

[0147] 3. Add 1mL oxidized dextran solution to every 1mL sericin solution;

[0148] 4. After fully stirring and mixing, place at 4°C, 25°C, and 37°C respectively to obtain sericin hydrogel SDH-3.

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Abstract

The present invention provides a sericin hydrogel and its preparation method and application. The preparation method comprises 1) extracting sericin, making it into powder, 2) preparing hydrazide-modified sericin, and making it 3) prepare oxidized dextran, and make it into powder, 4) prepare hydrazide-modified sericin solution and oxidized dextran solution, 5) mix hydrazide-modified sericin solution and oxidized dextran sugar solution to prepare sericin hydrogels. The sericin hydrogel prepared by the scheme of the present invention has good biodegradability and biocompatibility, and can be used as a drug carrier to carry a variety of macromolecular and small molecular drugs, and can be injected into the body by subcutaneous injection or intramuscular injection to release drugs . The hydrogel has fluorescence properties, and the degradation of the gel and the release of drugs can be monitored through fluorescence. And the sericin hydrogel can also be applied to skin repair. The sericin hydrogel scaffold is obtained through freeze-drying, which can also be used as a biomedical material.

Description

technical field [0001] The invention relates to the field of biomedical composite materials, in particular to a sericin hydrogel and its preparation method and application. Background technique [0002] Silk Sericin is a natural macromolecular protein wrapped on the surface of silk fibre. For a long time, due to the lack of understanding of sericin and the limitations of research, about 50,000 tons of sericin are treated as waste in the silk reeling industry every year, wasting a lot of valuable natural resources and causing serious damage to the environment. pollution. If the recycling of waste sericin is used as a green material in the field of tissue engineering and regenerative medicine, it may bring huge social, economic and environmental benefits. [0003] In recent years, in the field of tissue engineering, sericin has attracted more and more attention due to its good biological activities, such as biodegradability, low immunogenicity, antioxidant activity, and cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K47/36A61K9/06A61K49/00A61P35/00A61L27/26A61L27/52C07K1/14C07K1/107A61K31/704
Inventor 王琳王征刘佳乞超徐鲁明
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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