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Sericin hydrogel, preparation method and application thereof
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A technology of sericin and hydrogel, applied in the field of biomedical composite materials
Active Publication Date: 2017-01-18
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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[0004] So far, there has been no report that sericin extracted by high temperature or alkaline extraction has been prepared into injectable hydrogel by self-crosslinking
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Embodiment 1
[0107] Embodiment 1 Preparation of sericin hydrogel provided by the present invention
[0108] Selection of cocoons:
[0109] Wild-type silkworm cocoons (provided by the Institute of Sericulture, Chinese Academy of Agricultural Sciences) are selected, and the main chemical components are: sericin and silk fibroin.
[0110] Step 1), extract sericin and make it into powder
[0111] 1. Weigh wild-type silkworm cocoons (100g and cut into 1cm 2 The fragments were placed in a clean beaker, washed 3 times with ultrapure water, and centrifuged at 3500rpm for 5 minutes to remove water;
[0112] 2. Add 3 to 4 L of Na with a molar concentration of 0.02 mol / L to the silkworm cocoon fragments obtained in step 1 2 CO 3 solution or 3-4L of deionized water, put the beaker into a constant temperature water bath at 100°C for 1 hour to dissolve the sericin protein and obtain a sericin protein solution;
[0113] 3. Transfer the solution obtained in step 2 into a centrifuge tube and centrifug...
Embodiment 2
[0137] Example 2 Preparation of sericin hydrogel provided by the present invention
[0138] In the present embodiment, the preparation method of sericin solution is the same as that of Example 1, except that step 4) and step 5) are carried out as follows:
[0139] 1. Dissolve hydrazide-modified silk protein powder in ddH 2 In O, the concentration is adjusted to about 200g / L to obtain the hydrazide-modified sericin solution;
[0140] 2. Dissolve oxidized dextran powder in ddH 2 O, the concentration was adjusted to about 200g / L to obtain an oxidized dextran solution;
[0141] 3. Add 5mL oxidized dextran solution to every 1mL sericin solution;
[0142] 4. After fully stirring and mixing, place at 4°C, 25°C, and 37°C respectively to obtain sericin hydrogel SDH-2.
Embodiment 3
[0143] Example 3 Preparation of sericin hydrogel provided by the present invention
[0144] The difference between the preparation method of sericin hydrogel in this example and the preparation method of Example 1 is that step 4) and step 5) are carried out as follows:
[0145] 1. Dissolve hydrazide-modified silk protein powder in ddH 2 In O, the concentration is adjusted to about 200g / L to obtain the hydrazide-modified sericin solution;
[0146] 2. Dissolve oxidized dextran powder in ddH 2 O, the concentration was adjusted to about 350g / L to obtain an oxidized dextran solution;
[0147] 3. Add 1mL oxidized dextran solution to every 1mL sericin solution;
[0148] 4. After fully stirring and mixing, place at 4°C, 25°C, and 37°C respectively to obtain sericin hydrogel SDH-3.
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Abstract
The invention provides a sericin hydrogel, a preparation method and an application thereof. The preparation method comprises the following steps: 1) extracting sericin and preparing the sericin to powder, 2) preparing hydrazide-modified sericin and preparing the sericin to powder, 3) preparing oxidized glucan and preparing the glucan to powder, 4) preparing a hydrazide-modified sericin solution and an oxidative glucan solution, and 5) mixing the hydrazide-modified sericin solution and the oxidative glucan solution to prepare the sericin hydrogel. The sericin hydrogel has good biodegradability and biocompatibility, a plurality of macromolecular and micromolecular drugs are carried by taking sericin hydrogel as a drug carrier, and the sericin hydrogel can be injected into body for releasing the drugs through hypodermic injection or intramuscular injection. The hydrogel has fluorescence characteristic, can be degraded through fluorescence monitoring gel and can be realized. The sericin hydrogel can be used for skin restoration. The sericin hydrogel scaffold can be obtained through freeze drying, and can be used as a biomedical material.
Description
technical field [0001] The invention relates to the field of biomedical composite materials, in particular to a sericin hydrogel and its preparation method and application. Background technique [0002] Silk Sericin is a natural macromolecular protein wrapped on the surface of silk fibre. For a long time, due to the lack of understanding of sericin and the limitations of research, about 50,000 tons of sericin are treated as waste in the silk reeling industry every year, wasting a lot of valuable natural resources and causing serious damage to the environment. pollution. If the recycling of waste sericin is used as a green material in the field of tissue engineering and regenerative medicine, it may bring huge social, economic and environmental benefits. [0003] In recent years, in the field of tissue engineering, sericin has attracted more and more attention due to its good biological activities, such as biodegradability, low immunogenicity, antioxidant activity, and cell...
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