Method for collecting urine protein in large scale from human urine

A urine protein and large-scale technology, which is applied in the field of large-scale collection of urine protein, can solve the problems affecting the market development of urine protein products, the difficulty of urine collection, and the high cost of collection and transportation, so as to increase the single production batch and reduce the Effects of transportation cost and expansion of collection range

Inactive Publication Date: 2017-05-24
GUANGDONG TECHPOOL BIO-PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collecting urine in public toilets in cities with high population density, the collection and transportation costs are relatively high, and it is difficult to avoid urine spilling during the collection and transportation process, which has a certain impact on urban sanitation
[0005] At present, in the production of urine protein at home and abroad, it is necessary to collect urine first, and then extract urine protein, which is costly and has an impact on the environment. In addition to the creation of civilized and hygienic cities and urban sanitation, it is becoming more and more difficult to collect urine , which has seriously affected the market development of urinary protein products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Method for large-scale collection of human urine protein:

[0045] a) Place the pre-packed adsorbent for absorbing urine protein in the place where the urine flows through to absorb the urine protein in the urine;

[0046] b) Collect the adsorbent for adsorbing urinary protein, and add ice to cool when the ambient temperature is higher than 20°C during the process of collecting the adsorbent;

[0047] c) Before storing the adsorbent that absorbs urinary protein, perform pretreatment. The pretreatment method is to wash the adsorbent with high-pressure water, soak it in clean water, and dehydrate it, and dehydrate it with a centrifuge or a dehydrator; store the adsorbent in cold storage after dehydration ; Used to remove residual urine, microorganisms and urine scale from the adsorbent. It is used to prevent the denaturation and inactivation of urinary protein in the adsorbent, or even degrade it, and reduce the growth of microorganisms. According to the situation, the a...

Embodiment 2

[0051] On the basis of Example 1, when the preferred adsorbent device is an anion exchange resin, a urine filter pad is made. The urine filter pad is made of a nylon filter cloth with good permeability and waterproof, which is sewn into a bag and anion exchange resin in the bag. The preparation method is as follows: the suitable sieve size of the filter cloth is 300 mesh or less, and the pore size is more than 50 mesh, preferably 60 mesh pore size. The shape and size of the filter pad are compatible with the bottom of the urinal or trough, and can be square, round, polygonal, etc.; the side length or diameter of the filter pad used for the urinal is preferably 20-30cm; The filter pad used in the urinal is laid flat on the bottom of the small urinal, and its size should be able to fully cover the bottom of the urinal; the urine flows through the filter pad before flowing out of the urinal.

[0052] Take a macroporous anion exchange resin, for example, the ligand is {-N+(CH 3 ...

Embodiment 3

[0057] On the basis of Example 1, a 30cm×30cm size filter bag is sewn with a pore size of 80 mesh filter cloth, and the ligand used is -N(CH 3 ) 3 2 kilograms of the macroporous anion exchange resin styrene-diethylene copolymer of OH, through 2% NaOH treatment, water flushes to neutrality, packs in the filter cloth bag, makes urine filter pad.

[0058] Place the urine filter pad in the urine funnel, replace the filter pad for 24 hours, collect the resin, and desorb according to the method described in Example 1. The elution solution contained 1.28 grams of urinary protein.

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PUM

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Abstract

The invention relates to a method for collecting urine protein in a large scale from human urine. The method is especially suitable for large-scale use, and comprises the following steps: manufacturing a urine filtering device by use of an anion exchanger, and placing the urine filtering device in a urinal funnel or a urinal channel for carrying the urine flowing through the filtering device, wherein the shape and size of the urine filtering device are adaptive to those of the urinal funnel or the urinal channel; and recycling the urine filtering device at fixed time, desorbing and extracting the anion exchanger in concentration, extracting the component containing the urine protein, and performing the regeneration treatment on the anion exchanger so that the anion exchanger can be further used. The urine protein with various indexes achieving the pharmacopoeia requirements can be prepared through the subsequent purification treatment, and the urine protein can be used as the substitute goods of the human plasma source protein in multiple fields. The more important is that the corresponding pretreatment is performed before performing the treatment on the adsorbent absorbing the urine protein, so that the activity of the urine protein is guaranteed, the degradation of the urine protein and the propagation of microorganisms are reduced, and the quality of the urine protein product is improved.

Description

[0001] technology field or [0002] The invention relates to a method for large-scale collection of urinary protein from human urine, which is mainly suitable for the production of crude urine protein raw materials such as human urinary protein, human urinary trypsin inhibitor, human urinary kininogenase, and human urokinase. Background technique [0003] Human protein in human urine has the same molecular structure and biological properties as human albumin, and is the same substance (Poortmars, J. et al., Biochemica et Biophysica Acta, 43(1966), 380-389). Although the concentration of albumin in urine is very low, only about 10-20mg / L, the huge amount of urine sources makes the albumin content in it a huge amount. [0004] The practice of extracting protein from human urine has a long history in the medical field, and the most representative urine protein products are urokinase and human urinary kallikreinase. The traditional method of extracting urinary protein such as uro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/76C07K14/81C12N9/64C12N9/72C07K1/18C07K1/16C07K1/30
CPCC07K14/81C07K14/76C12N9/6445C12N9/6462C12Y304/21008C12Y304/21073
Inventor 王旭李溪亭何建国
Owner GUANGDONG TECHPOOL BIO-PHARMA CO LTD
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