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Method and Application of Surface Molecularly Imprinted Microspheres Prepared by Pickering Emulsion Polymerization Based on Hydrophobic Hydroxyapatite Nano-stabilized Particles

A technology of hydrophobic hydroxyapatite and surface molecular imprinting is applied in the preparation of microspheres, chemical instruments and methods, microcapsule preparations, etc. Simple, Bioactive Effects

Active Publication Date: 2018-09-11
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since various tissues and cells of organisms contain a variety of different proteins, it is not easy to obtain pure proteins. Therefore, the separation and purification of proteins is a complex and challenging task. The rapid development of bioengineering and medical technology The development has put forward higher requirements for the separation and purification of proteins
Traditional separation and purification methods such as salting out, chromatography, precipitation, dialysis, ultrafiltration, electrophoresis, etc. are limited in large-scale production, especially in the medical field, due to high cost and complicated process

Method used

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  • Method and Application of Surface Molecularly Imprinted Microspheres Prepared by Pickering Emulsion Polymerization Based on Hydrophobic Hydroxyapatite Nano-stabilized Particles
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  • Method and Application of Surface Molecularly Imprinted Microspheres Prepared by Pickering Emulsion Polymerization Based on Hydrophobic Hydroxyapatite Nano-stabilized Particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Weigh 5.0 g of hydroxyapatite, add it into a three-necked bottle containing 150 mL of anhydrous toluene, and disperse for 30 minutes with the aid of ultrasonic waves. 2 mL of 3-(methacryloyloxy)propyltrimethoxysilane was slowly added dropwise to the system, and then reacted at 120° C. for 1 day under the protection of nitrogen. After cooling to room temperature, the unreacted silane coupling agent was washed away by centrifugation with toluene, and the obtained sample was vacuum-dried at 60° C. for 2 days to obtain hydrophobic hydroxyapatite nanoparticles.

[0038] Weigh 170 mg of hydrophobic hydroxyapatite nanoparticles, add to 10 mL of toluene, and ultrasonically disperse for 30 minutes to obtain an oil phase. Weigh 20 mg of bovine hemoglobin, add it into 4 mL of PBS buffer (pH=7.4), oscillate to fully dissolve, then add 100 mg of dopamine hydrochloride, oscillate to fully dissolve, this is the water phase. The water phase and the oil phase were placed in the refrige...

Embodiment 2

[0050] Weigh 120 mg of hydrophobic hydroxyapatite nanoparticles (prepared in Example 1), add into 8 mL of acetonitrile, and ultrasonically disperse for 20 minutes to obtain an oil phase. Weigh 15 mg of bovine hemoglobin, add it into 4 mL of PBS buffer (pH=8.5), oscillate to fully dissolve, then add 100 mg of dopamine hydrochloride, oscillate to fully dissolve, this is the water phase. The water phase and the oil phase were placed in the refrigerator for 20 minutes respectively, then mixed and shaken vigorously to form a stable Pickering emulsion. The obtained emulsion was polymerized at room temperature for 5 hours, the product was washed with acetonitrile and pure water, and then the template molecule was removed with an eluent (SDS: 5%, acetic acid: 10%) to obtain a molecular imprint with a specific site Polymer (MIP), non-molecularly imprinted and polymeric (NIP) were prepared without adding bovine hemoglobin, and the other steps were exactly the same. The adsorption test ...

Embodiment 3

[0052] Weighed 200 mg of hydrophobic hydroxyapatite nanoparticles (prepared in Example 1), added into 15 mL of toluene, and ultrasonically dispersed for 50 minutes to obtain an oil phase. Weigh 20 mg of bovine serum albumin, add it to 7 mL of PBS buffer (pH=10.0), oscillate to fully dissolve, then add 100 mg of dopamine hydrochloride, oscillate to fully dissolve, this is the water phase. The water phase and the oil phase were placed in the refrigerator for 30 minutes, then mixed and shaken vigorously to form a stable Pickering emulsion. The obtained emulsion was polymerized at room temperature for 8 hours, the product was washed with toluene and purified water, and then the template molecule was removed with an eluent (SDS: 10%, acetic acid: 6%) to obtain a molecular imprint with a specific site Polymer (MIP), non-molecularly imprinted and polymer (NIP) were prepared without adding bovine serum albumin, and the other steps were exactly the same. The adsorption test was the sa...

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Abstract

The invention discloses a method for preparing surface molecular imprinting microspheres through polymerization of Pickering emulsion based on hydrophobic hydroxylapatite nanometer stable particles and application. The method for preparing the surface molecular imprinting microspheres comprises the steps that the hydrophobic hydroxylapatite nanometer particles are used as stable particles, protein is used as template molecules, polymeric microspheres are prepared through self-assembly of dopamine monomers in an aqueous phase, the template molecules are further removed through eluent, and the surface molecular imprinting microspheres can be obtained. Print sites are arranged on the surfaces of the surface molecular imprinting microspheres, the surface molecular imprinting microspheres can be in full contact with target molecules in a solution, efficient specific adsorption on the target molecules can be achieved, and the surface molecular imprinting microspheres can be widely applied to specific adsorption and separation of protein in a biosystem. According to the method, preparation is easy to grasp, preparation is easy and convenient, cost is low, and large-scale industrial production is greatly facilitated.

Description

technical field [0001] The present invention relates to a preparation method and application of surface molecularly imprinted microspheres, in particular to a method for preparing surface molecularly imprinted microspheres based on Pickering emulsion polymerization of hydroxyapatite nano-stabilized particles and its application in a specific adsorption solution system In particular, it relates to a kind of stable particle with hydrophobically modified hydroxyapatite, with dopamine as self-polymerization functional monomer, and bovine hemoglobin as template molecule, which is prepared by self-assembly polymerization of dopamine in Pickering emulsion The invention discloses a method for surface molecularly imprinted microspheres; it belongs to the fields of functional polymer preparation and bioseparation engineering. Background technique [0002] As an important basic component of organisms, protein plays an irreplaceable role in the entire life process of organisms. Studyin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G73/06C08K9/06C08K3/32C08J9/26B01J20/26B01J20/28B01J20/30B01J13/14
CPCB01J13/14B01J20/048B01J20/26B01J20/28021C08G73/0672C08J9/26C08J2201/0424C08K3/32C08K9/06C08K2003/325
Inventor 钟世安孙燕华
Owner CENT SOUTH UNIV
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